Restriction map for
pF1KE2472
(Circular) (Six-base) MAPSORT of: pF1KE2472.seq Check: 4679 from: 1 to: 7502
pF1KE2472 7502 bp
With 182 enzymes: SgfI *
June 18, 2013 11:58 ..
AccI GT'mk_AC
Cuts at: 4540 4540
Size: 7502
Acc65I G'GTAC_C
Cuts at: 2534 4518 2534
Size: 1984 5518
AclI AA'CG_TT
Cuts at: 7342 7342
Size: 7502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1267 1460 1831 2213 3104 3302 4208 4374
Size: 317 193 371 382 891 198 906 166
Cuts at: 4374 5327 5759 6506 6774 950
Size: 953 432 747 268 1678
Fragments arranged by size:
1678 953 906 891 747 432 382 371
317 268 198 193 166
AfeI AGC'GCT
Cuts at: 2702 2702
Size: 7502
AflIII A'CryG_T
Cuts at: 2977 4854 5958 6935 2977
Size: 1877 1104 977 3544
Fragments arranged by size:
3544 1877 1104 977
AgeI A'CCGG_T
Cuts at: 5890 5890
Size: 7502
AhdI GACnn_n'nnGTC
Cuts at: 2112 2112
Size: 7502
AleI CACnn'nnGTG
Cuts at: 520 3166 520
Size: 2646 4856
AlwNI CAG_nnn'CTG
Cuts at: 2122 4807 6374 2122
Size: 2685 1567 3250
Fragments arranged by size:
3250 2685 1567
ApaLI G'TGCA_C
Cuts at: 6272 6272
Size: 7502
ApoI r'AATT_y
Cuts at: 384 936 1068 1442 2209 2253 2281 2677 2761
Size: 552 132 374 767 44 28 396 84
Cuts at: 2761 3874 3940 4506 6855 7111 384
Size: 1113 66 566 2349 256 775
Fragments arranged by size:
2349 1113 775 767 566 552 396 374
256 132 84 66 44 28
AseI AT'TA_AT
Cuts at: 20 1345 3494 20
Size: 1325 2149 4028
Fragments arranged by size:
4028 2149 1325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
AvaI C'yCGr_G
Cuts at: 193 1900 4522 193
Size: 1707 2622 3173
Fragments arranged by size:
3173 2622 1707
BamHI G'GATC_C
Cuts at: 4181 4527 4181
Size: 346 7156
BanI G'GyrC_C
Cuts at: 150 2534 3676 3793 4518 4932 5180 5215 150
Size: 2384 1142 117 725 414 248 35 2437
Fragments arranged by size:
2437 2384 1142 725 414 248 117 35
BanII G_rGCy'C
Cuts at: 1060 1511 2220 2270 4516 5546 1060
Size: 451 709 50 2246 1030 3016
Fragments arranged by size:
3016 2246 1030 709 451 50
BbeI G_GCGC'C
Cuts at: 154 4936 5184 154
Size: 4782 248 2472
Fragments arranged by size:
4782 2472 248
BbsI GAAGACnn'nnnn_
Cuts at: 1010 3368 1010
Size: 2358 5144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 257 291 90
Size: 34 133 34 7301
Fragments arranged by size:
7301 133 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 5400 6161 7502 3455
Size: 1945 761 1341 3455
Fragments arranged by size:
3455 1945 1341 761
BclI T'GATC_A
Cuts at: 470 1191 1799 5022 470
Size: 721 608 3223 2950
Fragments arranged by size:
3223 2950 721 608
BfrBI ATG'CAT
Cuts at: 260 1408 3997 260
Size: 1148 2589 3765
Fragments arranged by size:
3765 2589 1148
BglI GCCn_nnn'nGGC
Cuts at: 1890 1890
Size: 7502
BglII A'GATC_T
Cuts at: 5017 7054 5017
Size: 2037 5465
BlpI GC'TnA_GC
Cuts at: 4614 4614
Size: 7502
Bme1580I G_kGCm'C
Cuts at: 5127 5220 6276 5127
Size: 93 1056 6353
Fragments arranged by size:
6353 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2118 2811 4131 4714 4795 5130 7274 2118
Size: 693 1320 583 81 335 2144 2346
Fragments arranged by size:
2346 2144 1320 693 583 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3732 3864 5869 3732
Size: 132 2005 5365
Fragments arranged by size:
5365 2005 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 310 1149 2790 226
Size: 84 839 1641 4938
Fragments arranged by size:
4938 1641 839 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1525 3303 4197 4501 4678 5557 6049 6347
Size: 1290 1778 894 304 177 879 492 298
Cuts at: 6347 235
Size: 1390
Fragments arranged by size:
1778 1390 1290 894 879 492 304 298
177
BsaI GGTCTCn'nnnn_
Cuts at: 3245 7206 3245
Size: 3961 3541
BsaAI yAC'GTr
Cuts at: 4855 4855
Size: 7502
BsaBI GATnn'nnATC
Cuts at: 6 3443 5016 7053 6
Size: 3437 1573 2037 455
Fragments arranged by size:
3437 2037 1573 455
BsaHI Gr'CG_yC
Cuts at: 151 4933 5181 5883 7379 151
Size: 4782 248 702 1496 274
Fragments arranged by size:
4782 1496 702 274 248
BsaWI w'CCGG_w
Cuts at: 4691 5212 5890 6164 6311 6722 4691
Size: 521 678 274 147 411 5471
Fragments arranged by size:
5471 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1146 3724 1146
Size: 2578 4924
BseYI C'CCAG_C
Cuts at: 144 1991 4927 5685 6262 6758 144
Size: 1847 2936 758 577 496 888
Fragments arranged by size:
2936 1847 888 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1733 1930 2620 2648 1733
Size: 197 690 28 6587
Fragments arranged by size:
6587 690 197 28
BsiEI CG_ry'CG
Cuts at: 104 5044 5090 6298 104
Size: 4940 46 1208 1308
Fragments arranged by size:
4940 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 953 2220 2270 3850 4516 5294 5484 6276 953
Size: 1267 50 1580 666 778 190 792 2179
Fragments arranged by size:
2179 1580 1267 792 778 666 190 50
BsmI GAATG_Cn'
Cuts at: 260 1317 2346 4059 4127 260
Size: 1057 1029 1713 68 3635
Fragments arranged by size:
3635 1713 1057 1029 68
Bsp1286I G_dGCh'C
Cuts at: 953 1060 1511 2220 2270 3850 4516 5127 5220
Size: 107 451 709 50 1580 666 611 93
Cuts at: 5220 5294 5484 5546 6276 953
Size: 74 190 62 730 2179
Fragments arranged by size:
2179 1580 730 709 666 611 451 190
107 93 74 62 50
BspEI T'CCGG_A
Cuts at: 6722 6722
Size: 7502
BspHI T'CATG_A
Cuts at: 675 1194 3936 6678 675
Size: 519 2742 2742 1499
Fragments arranged by size:
2742 2742 1499 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 259 854 3538 4552 4766 5068 5449 259
Size: 595 2684 1014 214 302 381 2312
Fragments arranged by size:
2684 2312 1014 595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 198 1038 5794 5848 7332 7497 50
Size: 148 840 4756 54 1484 165 55
Fragments arranged by size:
4756 1484 840 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2452 5414 2030
Size: 422 2962 4118
Fragments arranged by size:
4118 2962 422
BsrFI r'CCGG_y
Cuts at: 1905 2344 3605 5500 5681 5890 1905
Size: 439 1261 1895 181 209 3517
Fragments arranged by size:
3517 1895 1261 439 209 181
BssSI C'ACGA_G
Cuts at: 5773 6131 5773
Size: 358 7144
BstAPI GCAn_nnn'nTGC
Cuts at: 371 2233 3773 4879 371
Size: 1862 1540 1106 2994
Fragments arranged by size:
2994 1862 1540 1106
BstBI TT'CG_AA
Cuts at: 1707 5863 1707
Size: 4156 3346
BstXI CCAn_nnnn'nTGG
Cuts at: 1127 2848 3047 3628 1127
Size: 1721 199 581 5001
Fragments arranged by size:
5001 1721 581 199
BstYI r'GATC_y
Cuts at: 1 4181 4527 4994 5017 5352 5598 6610 6696
Size: 4180 346 467 23 335 246 1012 86
Cuts at: 6696 6708 7054 1
Size: 12 346 449
Fragments arranged by size:
4180 1012 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 154 342 627 154
Size: 188 285 7029
Fragments arranged by size:
7029 285 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5452 5633 5796 5452
Size: 181 163 7158
Fragments arranged by size:
7158 181 163
BtsI GCAGTG_nn'
Cuts at: 1024 4259 4745 1024
Size: 3235 486 3781
Fragments arranged by size:
3781 3235 486
DraI TTT'AAA
Cuts at: 3879 4501 3879
Size: 622 6880
DrdI GACnn_nn'nnGTC
Cuts at: 1477 5208 6066 1477
Size: 3731 858 2913
Fragments arranged by size:
3731 2913 858
EaeI y'GGCC_r
Cuts at: 2129 3153 4340 5087 5261 5652 5679 7024 2129
Size: 1024 1187 747 174 391 27 1345 2607
Fragments arranged by size:
2607 1345 1187 1024 747 391 174 27
EagI C'GGCC_G
Cuts at: 5087 5087
Size: 7502
EarI CTCTTCn'nnn_
Cuts at: 1479 1850 2069 2243 2388 2808 2882 4004 4480
Size: 371 219 174 145 420 74 1122 476
Cuts at: 4480 5525 5735 1479
Size: 1045 210 3246
Fragments arranged by size:
3246 1122 1045 476 420 371 219 210
174 145 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 381 5710 6020 6166 7312 381
Size: 5329 310 146 1146 571
Fragments arranged by size:
5329 1146 571 310 146
EcoICRI GAG'CTC
Cuts at: 2218 2268 4514 2218
Size: 50 2246 5206
Fragments arranged by size:
5206 2246 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 310 950 1149 1267 1460 1831 2213 2790
Size: 84 640 199 118 193 371 382 577
Cuts at: 2790 3104 3302 4208 4374 5327 5759 6506 6774
Size: 314 198 906 166 953 432 747 268
Cuts at: 6774 226
Size: 954
Fragments arranged by size:
954 953 906 747 640 577 432 382
371 314 268 199 198 193 166 118
84
EcoO109I rG'GnC_Cy
Cuts at: 813 3095 4641 813
Size: 2282 1546 3674
Fragments arranged by size:
3674 2282 1546
EcoRI G'AATT_C
Cuts at: 2677 4506 2677
Size: 1829 5673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 5521 1612
Size: 3909 3593
FspI TGC'GCA
Cuts at: 5283 5283
Size: 7502
HaeII r_GCGC'y
Cuts at: 154 187 456 2704 4273 4936 5184 6206 7185
Size: 33 269 2248 1569 663 248 1022 979
Cuts at: 7185 154
Size: 471
Fragments arranged by size:
2248 1569 1022 979 663 471 269 248
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 1132 1164 1342 1374 1627 1659 2098
Size: 32 340 32 178 32 253 32 439
Cuts at: 2098 2130 3051 3083 3697 3729 5344 5374 5376
Size: 32 921 32 614 32 1615 30 2
Cuts at: 5376 5406 760
Size: 30 2856
Fragments arranged by size:
2856 1615 921 614 439 340 253 178
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4541 4541
Size: 7502
HindIII A'AGCT_T
Cuts at: 411 1710 1721 2229 3128 411
Size: 1299 11 508 899 4785
Fragments arranged by size:
4785 1299 899 508 11
KasI G'GCGC_C
Cuts at: 150 4932 5180 150
Size: 4782 248 2472
Fragments arranged by size:
4782 2472 248
KpnI G_GTAC'C
Cuts at: 2538 4522 2538
Size: 1984 5518
MluI A'CGCG_T
Cuts at: 6935 6935
Size: 7502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3028 6173 6357 3028
Size: 3145 184 4173
Fragments arranged by size:
4173 3145 184
MscI TGG'CCA
Cuts at: 2131 3155 4342 5263 2131
Size: 1024 1187 921 4370
Fragments arranged by size:
4370 1187 1024 921
MslI CAynn'nnrTG
Cuts at: 520 1164 2355 3077 3139 3166 4166 4379 5491
Size: 644 1191 722 62 27 1000 213 1112
Cuts at: 5491 5618 520
Size: 127 2404
Fragments arranged by size:
2404 1191 1112 1000 722 644 213 127
62 27
MspA1I CmG'CkG
Cuts at: 156 368 483 996 1536 1730 4613 4927 5287
Size: 212 115 513 540 194 2883 314 360
Cuts at: 5287 6300 6545 156
Size: 1013 245 1113
Fragments arranged by size:
2883 1113 1013 540 513 360 314 245
212 194 115
NaeI GCC'GGC
Cuts at: 2346 5683 2346
Size: 3337 4165
NarI GG'CG_CC
Cuts at: 151 4933 5181 151
Size: 4782 248 2472
Fragments arranged by size:
4782 2472 248
NcoI C'CATG_G
Cuts at: 342 342
Size: 7502
NgoMIV G'CCGG_C
Cuts at: 2344 5681 2344
Size: 3337 4165
NsiI A_TGCA'T
Cuts at: 262 1410 3999 262
Size: 1148 2589 3765
Fragments arranged by size:
3765 2589 1148
NspI r_CATG'y
Cuts at: 321 3067 4555 5055 5962 321
Size: 2746 1488 500 907 1861
Fragments arranged by size:
2746 1861 1488 907 500
PciI A'CATG_T
Cuts at: 5958 5958
Size: 7502
PflMI CCAn_nnn'nTGG
Cuts at: 2990 3170 4088 2990
Size: 180 918 6404
Fragments arranged by size:
6404 918 180
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 7502
PmeI GTTT'AAAC
Cuts at: 4501 4501
Size: 7502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6641 6673 6641
Size: 32 7470
PpuMI rG'GwC_Cy
Cuts at: 813 3095 813
Size: 2282 5220
PshAI GACnn'nnGTC
Cuts at: 3100 3100
Size: 7502
PsiI TTA'TAA
Cuts at: 2438 2438
Size: 7502
PstI C_TGCA'G
Cuts at: 488 783 4266 4549 5234 488
Size: 295 3483 283 685 2756
Fragments arranged by size:
3483 2756 685 295 283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7502
PvuII CAG'CTG
Cuts at: 996 1536 4927 5287 996
Size: 540 3391 360 3211
Fragments arranged by size:
3391 3211 540 360
RsrII CG'GwC_CG
Cuts at: 5697 5697
Size: 7502
SacI G_AGCT'C
Cuts at: 2220 2270 4516 2220
Size: 50 2246 5206
Fragments arranged by size:
5206 2246 50
SacII CC_GC'GG
Cuts at: 157 157
Size: 7502
SalI G'TCGA_C
Cuts at: 4539 4539
Size: 7502
SanDI GG'GwC_CC
Cuts at: 3095 3095
Size: 7502
SapI GCTCTTCn'nnn_
Cuts at: 1850 4004 5525 5735 1850
Size: 2154 1521 210 3617
Fragments arranged by size:
3617 2154 1521 210
SbfI CC_TGCA'GG
Cuts at: 4549 4549
Size: 7502
ScaI AGT'ACT
Cuts at: 490 7019 490
Size: 6529 973
SfcI C'TryA_G
Cuts at: 33 484 779 1944 2625 3891 3902 4262 4545
Size: 451 295 1165 681 1266 11 360 283
Cuts at: 4545 5230 6223 6414 33
Size: 685 993 191 1121
Fragments arranged by size:
1266 1165 1121 993 685 681 451 360
295 283 191 11
SfoI GGC'GCC
Cuts at: 152 4934 5182 152
Size: 4782 248 2472
Fragments arranged by size:
4782 2472 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7502
SmaI CCC'GGG
Cuts at: 4524 4524
Size: 7502
SmlI C'TyrA_G
Cuts at: 250 1504 3318 4176 4480 4657 5572 6064 6326
Size: 1254 1814 858 304 177 915 492 262
Cuts at: 6326 250
Size: 1426
Fragments arranged by size:
1814 1426 1254 915 858 492 304 262
177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7502
SphI G_CATG'C
Cuts at: 3067 4555 5055 3067
Size: 1488 500 5514
Fragments arranged by size:
5514 1488 500
SspI AAT'ATT
Cuts at: 227 1225 3261 4464 227
Size: 998 2036 1203 3265
Fragments arranged by size:
3265 2036 1203 998
StyI C'CwwG_G
Cuts at: 342 1392 3723 4636 4990 342
Size: 1050 2331 913 354 2854
Fragments arranged by size:
2854 2331 1050 913 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 969 3851 5085 5218 5677 5886 640
Size: 329 2882 1234 133 459 209 2256
Fragments arranged by size:
2882 2256 1234 459 329 209 133
TatI w'GTAC_w
Cuts at: 488 1913 4317 4441 7017 488
Size: 1425 2404 124 2576 973
Fragments arranged by size:
2576 2404 1425 973 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 2771 2803 3206 3238 7019 7051 2458
Size: 32 281 32 403 32 3781 32 2909
Fragments arranged by size:
3781 2909 403 281 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1699 4289 5299 1699
Size: 2590 1010 3902
Fragments arranged by size:
3902 2590 1010
XbaI T'CTAG_A
Cuts at: 4533 4533
Size: 7502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1243 1988 4997 1243
Size: 745 3009 3748
Fragments arranged by size:
3748 3009 745
XmaI C'CCGG_G
Cuts at: 4522 4522
Size: 7502
XmnI GAAnn'nnTTC
Cuts at: 2162 4463 2162
Size: 2301 5201
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PmlI PspOMI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI