Restriction map for
pF1KE2468
(Circular) (Six-base) MAPSORT of: pF1KE2468.seq Check: 6640 from: 1 to: 4817
pF1KE2468 4817 bp
With 182 enzymes: SgfI *
April 17, 2013 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 327 327
Size: 4817
AccI GT'mk_AC
Cuts at: 1855 1855
Size: 4817
Acc65I G'GTAC_C
Cuts at: 1833 1833
Size: 4817
AclI AA'CG_TT
Cuts at: 4657 4657
Size: 4817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3074 3821 4089 2642
Size: 432 747 268 3370
Fragments arranged by size:
3370 747 432 268
AfeI AGC'GCT
Cuts at: 412 412
Size: 4817
AflIII A'CryG_T
Cuts at: 2169 3273 4250 2169
Size: 1104 977 2736
Fragments arranged by size:
2736 1104 977
AgeI A'CCGG_T
Cuts at: 3205 3205
Size: 4817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1357 1357
Size: 4817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 844
Size: 32 4785
AlwNI CAG_nnn'CTG
Cuts at: 324 2122 3689 324
Size: 1798 1567 1452
Fragments arranged by size:
1798 1567 1452
ApaI G_GGCC'C
Cuts at: 1040 1040
Size: 4817
ApaLI G'TGCA_C
Cuts at: 255 1491 3587 255
Size: 1236 2096 1485
Fragments arranged by size:
2096 1485 1236
ApoI r'AATT_y
Cuts at: 1821 4170 4426 1821
Size: 2349 256 2212
Fragments arranged by size:
2349 2212 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
AvaI C'yCGr_G
Cuts at: 1103 1386 1837 1103
Size: 283 451 4083
Fragments arranged by size:
4083 451 283
BamHI G'GATC_C
Cuts at: 1375 1842 1375
Size: 467 4350
BanI G'GyrC_C
Cuts at: 552 766 1661 1833 2247 2495 2530 552
Size: 214 895 172 414 248 35 2839
Fragments arranged by size:
2839 895 414 248 214 172 35
BanII G_rGCy'C
Cuts at: 1040 1424 1831 2861 1040
Size: 384 407 1030 2996
Fragments arranged by size:
2996 1030 407 384
BbeI G_GCGC'C
Cuts at: 2251 2499 2251
Size: 248 4569
BbsI GAAGACnn'nnnn_
Cuts at: 704 1710 704
Size: 1006 3811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 828 862 979 1013 828
Size: 34 117 34 4632
Fragments arranged by size:
4632 117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2715 3476 4817 2715
Size: 761 1341 2715
Fragments arranged by size:
2715 1341 761
BclI T'GATC_A
Cuts at: 2337 2337
Size: 4817
BglI GCCn_nnn'nGGC
Cuts at: 474 607 1128 1183 474
Size: 133 521 55 4108
Fragments arranged by size:
4108 521 133 55
BglII A'GATC_T
Cuts at: 2332 4369 2332
Size: 2037 2780
BlpI GC'TnA_GC
Cuts at: 121 1929 121
Size: 1808 3009
Bme1580I G_kGCm'C
Cuts at: 259 557 769 1040 1328 1439 1495 1664 2442
Size: 298 212 271 288 111 56 169 778
Cuts at: 2442 2535 3591 259
Size: 93 1056 1485
Fragments arranged by size:
1485 1056 778 298 288 271 212 169
111 93 56
BmrI ACTGGGnnnn_n'
Cuts at: 1794 2029 2110 2445 4589 1794
Size: 235 81 335 2144 2022
Fragments arranged by size:
2144 2022 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 4817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 637 907 315
Size: 322 270 4225
Fragments arranged by size:
4225 322 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1651 1993 2872 3364 3662 988
Size: 663 342 879 492 298 2143
Fragments arranged by size:
2143 879 663 492 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 1100 4521 1100
Size: 3421 1396
BsaAI yAC'GTr
Cuts at: 2170 2170
Size: 4817
BsaBI GATnn'nnATC
Cuts at: 6 2331 4368 6
Size: 2325 2037 455
Fragments arranged by size:
2325 2037 455
BsaHI Gr'CG_yC
Cuts at: 497 2248 2496 3198 4694 497
Size: 1751 248 702 1496 620
Fragments arranged by size:
1751 1496 702 620 248
BsaWI w'CCGG_w
Cuts at: 1025 2006 2527 3205 3479 3626 4037 1025
Size: 981 521 678 274 147 411 1805
Fragments arranged by size:
1805 981 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 1033 439
Size: 594 4223
BseYI C'CCAG_C
Cuts at: 483 1032 2242 3000 3577 4073 483
Size: 549 1210 758 577 496 1227
Fragments arranged by size:
1227 1210 758 577 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 455 406
Size: 49 4768
BsiEI CG_ry'CG
Cuts at: 104 2359 2405 3613 104
Size: 2255 46 1208 1308
Fragments arranged by size:
2255 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 259 806 1495 1831 2609 2799 3591 259
Size: 547 689 336 778 190 792 1485
Fragments arranged by size:
1485 792 778 689 547 336 190
Bsp1286I G_dGCh'C
Cuts at: 259 557 769 806 1040 1328 1424 1439 1495
Size: 298 212 37 234 288 96 15 56
Cuts at: 1495 1664 1831 2442 2535 2609 2799 2861 3591
Size: 169 167 611 93 74 190 62 730
Cuts at: 3591 259
Size: 1485
Fragments arranged by size:
1485 730 611 298 288 234 212 190
169 167 96 93 74 62 56 37
15
BspEI T'CCGG_A
Cuts at: 4037 4037
Size: 4817
BspHI T'CATG_A
Cuts at: 3993 3993
Size: 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 428 1867 2081 2383 2764 327
Size: 101 1439 214 302 381 2380
Fragments arranged by size:
2380 1439 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 171 708 3109 3163 4647 4812 50
Size: 121 537 2401 54 1484 165 55
Fragments arranged by size:
2401 1484 537 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 2729 2729
Size: 4817
BsrFI r'CCGG_y
Cuts at: 610 2815 2996 3205 610
Size: 2205 181 209 2222
Fragments arranged by size:
2222 2205 209 181
BsrGI T'GTAC_A
Cuts at: 1571 1571
Size: 4817
BssSI C'ACGA_G
Cuts at: 847 3088 3446 847
Size: 2241 358 2218
Fragments arranged by size:
2241 2218 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1434 2194 1434
Size: 760 4057
BstBI TT'CG_AA
Cuts at: 3178 3178
Size: 4817
BstEII G'GTnAC_C
Cuts at: 1308 1308
Size: 4817
BstXI CCAn_nnnn'nTGG
Cuts at: 891 1194 891
Size: 303 4514
BstYI r'GATC_y
Cuts at: 1 1375 1842 2309 2332 2667 2913 3925 4011
Size: 1374 467 467 23 335 246 1012 86
Cuts at: 4011 4023 4369 1
Size: 12 346 449
Fragments arranged by size:
1374 1012 467 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 398 539 107
Size: 291 141 4385
Fragments arranged by size:
4385 291 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2767 2948 3111 2767
Size: 181 163 4473
Fragments arranged by size:
4473 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 2060 1439
Size: 621 4196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1484 1519 1484
Size: 35 4782
DraI TTT'AAA
Cuts at: 951 1816 951
Size: 865 3952
DraIII CAC_nnn'GTG
Cuts at: 1434 1490 1434
Size: 56 4761
DrdI GACnn_nn'nnGTC
Cuts at: 2523 3381 2523
Size: 858 3959
EaeI y'GGCC_r
Cuts at: 242 395 542 752 1184 1196 1505 1625 2402
Size: 153 147 210 432 12 309 120 777
Cuts at: 2402 2576 2967 2994 4339 242
Size: 174 391 27 1345 720
Fragments arranged by size:
1345 777 720 432 391 309 210 174
153 147 120 27 12
EagI C'GGCC_G
Cuts at: 2402 2402
Size: 4817
EarI CTCTTCn'nnn_
Cuts at: 319 706 2840 3050 319
Size: 387 2134 210 2086
Fragments arranged by size:
2134 2086 387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1370 3025 3335 3481 4627 1370
Size: 1655 310 146 1146 1560
Fragments arranged by size:
1655 1560 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1829 1829
Size: 4817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 637 907 2642 3074 3821 4089 315
Size: 322 270 1735 432 747 268 1043
Fragments arranged by size:
1735 1043 747 432 322 270 268
EcoO109I rG'GnC_Cy
Cuts at: 676 790 843 1036 1037 1956 676
Size: 114 53 193 1 919 3537
Fragments arranged by size:
3537 919 193 114 53 1
EcoRI G'AATT_C
Cuts at: 1821 1821
Size: 4817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1257 2836 1257
Size: 1579 3238
FseI GG_CCGG'CC
Cuts at: 614 614
Size: 4817
FspI TGC'GCA
Cuts at: 442 2598 442
Size: 2156 2661
HaeII r_GCGC'y
Cuts at: 121 414 2251 2499 3521 4500 121
Size: 293 1837 248 1022 979 438
Fragments arranged by size:
1837 1022 979 438 293 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2659 2689 2691 2721 2659
Size: 30 2 30 4755
Fragments arranged by size:
4755 30 30 2
HincII GTy'rAC
Cuts at: 1740 1856 1740
Size: 116 4701
KasI G'GCGC_C
Cuts at: 2247 2495 2247
Size: 248 4569
KpnI G_GTAC'C
Cuts at: 1837 1837
Size: 4817
MluI A'CGCG_T
Cuts at: 4250 4250
Size: 4817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3488 3672 3488
Size: 184 4633
MscI TGG'CCA
Cuts at: 244 1186 1198 1507 1627 2578 244
Size: 942 12 309 120 951 2483
Fragments arranged by size:
2483 951 942 309 120 12
MslI CAynn'nnrTG
Cuts at: 196 238 367 544 550 892 1192 2806 2933
Size: 42 129 177 6 342 300 1614 127
Cuts at: 2933 196
Size: 2080
Fragments arranged by size:
2080 1614 342 300 177 129 127 42
6
MspA1I CmG'CkG
Cuts at: 1569 1928 2242 2602 3615 3860 1569
Size: 359 314 360 1013 245 2526
Fragments arranged by size:
2526 1013 360 359 314 245
NaeI GCC'GGC
Cuts at: 612 2998 612
Size: 2386 2431
NarI GG'CG_CC
Cuts at: 2248 2496 2248
Size: 248 4569
NcoI C'CATG_G
Cuts at: 107 107
Size: 4817
NgoMIV G'CCGG_C
Cuts at: 610 2996 610
Size: 2386 2431
NspI r_CATG'y
Cuts at: 372 891 1870 2370 3277 372
Size: 519 979 500 907 1912
Fragments arranged by size:
1912 979 907 519 500
PasI CC'CwG_GG
Cuts at: 1328 1328
Size: 4817
PciI A'CATG_T
Cuts at: 3273 3273
Size: 4817
PflMI CCAn_nnn'nTGG
Cuts at: 251 324 251
Size: 73 4744
PmeI GTTT'AAAC
Cuts at: 1816 1816
Size: 4817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4785
PpuMI rG'GwC_Cy
Cuts at: 790 843 790
Size: 53 4764
PspOMI G'GGCC_C
Cuts at: 1036 1036
Size: 4817
PstI C_TGCA'G
Cuts at: 1016 1864 2549 1016
Size: 848 685 3284
Fragments arranged by size:
3284 848 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4817
PvuII CAG'CTG
Cuts at: 2242 2602 2242
Size: 360 4457
RsrII CG'GwC_CG
Cuts at: 3012 3012
Size: 4817
SacI G_AGCT'C
Cuts at: 1831 1831
Size: 4817
SalI G'TCGA_C
Cuts at: 1854 1854
Size: 4817
SanDI GG'GwC_CC
Cuts at: 790 790
Size: 4817
SapI GCTCTTCn'nnn_
Cuts at: 2840 3050 2840
Size: 210 4607
SbfI CC_TGCA'GG
Cuts at: 1864 1864
Size: 4817
ScaI AGT'ACT
Cuts at: 4334 4334
Size: 4817
SfcI C'TryA_G
Cuts at: 33 1012 1860 2545 3538 3729 33
Size: 979 848 685 993 191 1121
Fragments arranged by size:
1121 993 979 848 685 191
SfoI GGC'GCC
Cuts at: 2249 2497 2249
Size: 248 4569
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
SmaI CCC'GGG
Cuts at: 1839 1839
Size: 4817
SmlI C'TyrA_G
Cuts at: 1003 1666 1972 2887 3379 3641 1003
Size: 663 306 915 492 262 2179
Fragments arranged by size:
2179 915 663 492 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4817
SphI G_CATG'C
Cuts at: 1870 2370 1870
Size: 500 4317
StuI AGG'CCT
Cuts at: 1384 1384
Size: 4817
StyI C'CwwG_G
Cuts at: 107 641 1951 2305 107
Size: 534 1310 354 2619
Fragments arranged by size:
2619 1310 534 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 1134 2400 2533 2992 3201 444
Size: 690 1266 133 459 209 2060
Fragments arranged by size:
2060 1266 690 459 209 133
TatI w'GTAC_w
Cuts at: 350 1571 4332 350
Size: 1221 2761 835
Fragments arranged by size:
2761 1221 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 782 814 4334 4366 782
Size: 32 3520 32 1233
Fragments arranged by size:
3520 1233 32 32
Tth111I GACn'n_nGTC
Cuts at: 2614 2614
Size: 4817
XbaI T'CTAG_A
Cuts at: 1848 1848
Size: 4817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 1764 2312 546
Size: 1218 548 3051
Fragments arranged by size:
3051 1218 548
XmaI C'CCGG_G
Cuts at: 1837 1837
Size: 4817
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstZ17I Bsu36I ClaI EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI