Restriction map for
pF1KE2467
(Circular) (Six-base) MAPSORT of: pF1KE2467.seq Check: 2388 from: 1 to: 3806
pF1KE2467 3806 bp
With 182 enzymes: SgfI *
March 29, 2013 15:36 ..
AccI GT'mk_AC
Cuts at: 389 844 389
Size: 455 3351
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2063 2810 3078 1631
Size: 432 747 268 2359
Fragments arranged by size:
2359 747 432 268
AflIII A'CryG_T
Cuts at: 1158 2262 3239 1158
Size: 1104 977 1725
Fragments arranged by size:
1725 1104 977
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AlwNI CAG_nnn'CTG
Cuts at: 1111 2678 1111
Size: 1567 2239
ApaLI G'TGCA_C
Cuts at: 2576 2576
Size: 3806
ApoI r'AATT_y
Cuts at: 129 628 810 3159 3415 129
Size: 499 182 2349 256 520
Fragments arranged by size:
2349 520 499 256 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 826 826
Size: 3806
BamHI G'GATC_C
Cuts at: 831 831
Size: 3806
BanI G'GyrC_C
Cuts at: 822 1236 1484 1519 822
Size: 414 248 35 3109
Fragments arranged by size:
3109 414 248 35
BanII G_rGCy'C
Cuts at: 709 820 1850 709
Size: 111 1030 2665
Fragments arranged by size:
2665 1030 111
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2465 3806 1704
Size: 761 1341 1704
Fragments arranged by size:
1704 1341 761
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BglII A'GATC_T
Cuts at: 1321 3358 1321
Size: 2037 1769
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 780 1431 1524 2580 780
Size: 651 93 1056 2006
Fragments arranged by size:
2006 1056 651 93
BmrI ACTGGGnnnn_n'
Cuts at: 190 1018 1099 1434 3578 190
Size: 828 81 335 2144 418
Fragments arranged by size:
2144 828 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 3806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 982 1861 2353 2651 218
Size: 764 879 492 298 1373
Fragments arranged by size:
1373 879 764 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 429 3510 429
Size: 3081 725
BsaAI yAC'GTr
Cuts at: 387 1159 387
Size: 772 3034
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 995 1516 2194 2468 2615 3026 995
Size: 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1231 1989 2566 3062 1231
Size: 758 577 496 1975
Fragments arranged by size:
1975 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 784 1348 1394 2602 104
Size: 680 564 46 1208 1308
Fragments arranged by size:
1308 1208 680 564 46
BsiHKAI G_wGCw'C
Cuts at: 820 1598 1788 2580 820
Size: 778 190 792 2046
Fragments arranged by size:
2046 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 709 780 820 1431 1524 1598 1788 1850 2580
Size: 71 40 611 93 74 190 62 730
Cuts at: 2580 709
Size: 1935
Fragments arranged by size:
1935 730 611 190 93 74 71 62
40
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 856 1070 1372 1753 856
Size: 214 302 381 2909
Fragments arranged by size:
2909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 192 1718 192
Size: 1526 2280
BsrFI r'CCGG_y
Cuts at: 783 1804 1985 2194 783
Size: 1021 181 209 2395
Fragments arranged by size:
2395 1021 209 181
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 1183 1183
Size: 3806
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstYI r'GATC_y
Cuts at: 1 519 831 1298 1321 1656 1902 2914 3000
Size: 518 312 467 23 335 246 1012 86
Cuts at: 3000 3012 3358 1
Size: 12 346 449
Fragments arranged by size:
1012 518 467 449 346 335 312 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 772 772
Size: 3806
BtgI C'CryG_G
Cuts at: 107 107
Size: 3806
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 254 1049 254
Size: 795 3011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 660 695 660
Size: 35 3771
DraI TTT'AAA
Cuts at: 805 805
Size: 3806
DrdI GACnn_nn'nnGTC
Cuts at: 1512 2370 1512
Size: 858 2948
EaeI y'GGCC_r
Cuts at: 531 781 1391 1565 1956 1983 3328 531
Size: 250 610 174 391 27 1345 1009
Fragments arranged by size:
1345 1009 610 391 250 174 27
EagI C'GGCC_G
Cuts at: 781 1391 781
Size: 610 3196
EarI CTCTTCn'nnn_
Cuts at: 199 427 1829 2039 199
Size: 228 1402 210 1966
Fragments arranged by size:
1966 1402 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 818 818
Size: 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2063 2810 3078 1631
Size: 432 747 268 2359
Fragments arranged by size:
2359 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 945 945
Size: 3806
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 1240 1488 2510 3489 1240
Size: 248 1022 979 1557
Fragments arranged by size:
1557 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1648 1678 1680 1710 1648
Size: 30 2 30 3744
Fragments arranged by size:
3744 30 30 2
HincII GTy'rAC
Cuts at: 465 669 845 465
Size: 204 176 3426
Fragments arranged by size:
3426 204 176
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2661 2477
Size: 184 3622
MscI TGG'CCA
Cuts at: 533 1567 533
Size: 1034 2772
MslI CAynn'nnrTG
Cuts at: 760 1795 1922 760
Size: 1035 127 2644
Fragments arranged by size:
2644 1035 127
MspA1I CmG'CkG
Cuts at: 917 1231 1591 2604 2849 917
Size: 314 360 1013 245 1874
Fragments arranged by size:
1874 1013 360 314 245
NaeI GCC'GGC
Cuts at: 785 1987 785
Size: 1202 2604
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NcoI C'CATG_G
Cuts at: 107 107
Size: 3806
NgoMIV G'CCGG_C
Cuts at: 783 1985 783
Size: 1202 2604
NspI r_CATG'y
Cuts at: 692 859 1359 2266 692
Size: 167 500 907 2232
Fragments arranged by size:
2232 907 500 167
PciI A'CATG_T
Cuts at: 2262 2262
Size: 3806
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PstI C_TGCA'G
Cuts at: 853 1538 853
Size: 685 3121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 1231 1591 1231
Size: 360 3446
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 820 820
Size: 3806
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 1829 2039 1829
Size: 210 3596
SbfI CC_TGCA'GG
Cuts at: 853 853
Size: 3806
ScaI AGT'ACT
Cuts at: 3323 3323
Size: 3806
SfcI C'TryA_G
Cuts at: 33 410 849 1534 2527 2718 33
Size: 377 439 685 993 191 1121
Fragments arranged by size:
1121 993 685 439 377 191
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SmaI CCC'GGG
Cuts at: 828 828
Size: 3806
SmlI C'TyrA_G
Cuts at: 233 961 1876 2368 2630 233
Size: 728 915 492 262 1409
Fragments arranged by size:
1409 915 728 492 262
SnaBI TAC'GTA
Cuts at: 387 387
Size: 3806
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 859 1359 859
Size: 500 3306
StyI C'CwwG_G
Cuts at: 107 940 1294 107
Size: 833 354 2619
Fragments arranged by size:
2619 833 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 1389 1522 1981 2190 229
Size: 1160 133 459 209 1845
Fragments arranged by size:
1845 1160 459 209 133
TatI w'GTAC_w
Cuts at: 3321 3321
Size: 3806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 585 1301 585
Size: 716 3090
XmaI C'CCGG_G
Cuts at: 826 826
Size: 3806
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI