Restriction map for
pF1KE2464
(Circular) (Six-base) MAPSORT of: pF1KE2464.seq Check: 6305 from: 1 to: 5402
pF1KE2464 5402 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2073 2073
Size: 5402
AccI GT'mk_AC
Cuts at: 2440 2440
Size: 5402
Acc65I G'GTAC_C
Cuts at: 2418 2418
Size: 5402
AclI AA'CG_TT
Cuts at: 5242 5242
Size: 5402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 340 478 859 1093 1235 1462 1525 1738
Size: 161 138 381 234 142 227 63 213
Cuts at: 1738 1921 3227 3659 4406 4674 179
Size: 183 1306 432 747 268 907
Fragments arranged by size:
1306 907 747 432 381 268 234 227
213 183 161 142 138 63
AflIII A'CryG_T
Cuts at: 1179 2327 2754 3858 4835 1179
Size: 1148 427 1104 977 1746
Fragments arranged by size:
1746 1148 1104 977 427
AgeI A'CCGG_T
Cuts at: 3790 3790
Size: 5402
AlwNI CAG_nnn'CTG
Cuts at: 687 916 1503 2707 4274 687
Size: 229 587 1204 1567 1815
Fragments arranged by size:
1815 1567 1204 587 229
ApaLI G'TGCA_C
Cuts at: 4172 4172
Size: 5402
ApoI r'AATT_y
Cuts at: 2406 4755 5011 2406
Size: 2349 256 2797
Fragments arranged by size:
2797 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5402
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
AvaI C'yCGr_G
Cuts at: 1489 2422 1489
Size: 933 4469
BamHI G'GATC_C
Cuts at: 2427 2427
Size: 5402
BanI G'GyrC_C
Cuts at: 2418 2832 3080 3115 2418
Size: 414 248 35 4705
Fragments arranged by size:
4705 414 248 35
BanII G_rGCy'C
Cuts at: 872 1134 2416 3446 872
Size: 262 1282 1030 2828
Fragments arranged by size:
2828 1282 1030 262
BbeI G_GCGC'C
Cuts at: 2836 3084 2836
Size: 248 5154
BbsI GAAGACnn'nnnn_
Cuts at: 1370 1570 1370
Size: 200 5202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1147 1181 1803 1837 1147
Size: 34 622 34 4712
Fragments arranged by size:
4712 622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 591 3300 4061 5402 591
Size: 2709 761 1341 591
Fragments arranged by size:
2709 1341 761 591
BclI T'GATC_A
Cuts at: 2922 2922
Size: 5402
BglII A'GATC_T
Cuts at: 1320 2917 4954 1320
Size: 1597 2037 1768
Fragments arranged by size:
2037 1768 1597
BlpI GC'TnA_GC
Cuts at: 688 1169 2161 2514 688
Size: 481 992 353 3576
Fragments arranged by size:
3576 992 481 353
Bme1580I G_kGCm'C
Cuts at: 1944 3027 3120 4176 1944
Size: 1083 93 1056 3170
Fragments arranged by size:
3170 1083 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2614 2695 3030 5174 2614
Size: 81 335 2144 2842
Fragments arranged by size:
2842 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 828 3769 828
Size: 2941 2461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 394 415 1039 1260 1366 1447 1864 1936
Size: 198 21 624 221 106 81 417 72
Cuts at: 1936 1948 2032 2278 196
Size: 12 84 246 3320
Fragments arranged by size:
3320 624 417 246 221 198 106 84
81 72 21 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1303 2170 2578 3457 3949 4247 884
Size: 419 867 408 879 492 298 2039
Fragments arranged by size:
2039 879 867 492 419 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 213 697 716 1088 1271 2146 5106 213
Size: 484 19 372 183 875 2960 509
Fragments arranged by size:
2960 875 509 484 372 183 19
BsaAI yAC'GTr
Cuts at: 1182 2755 1182
Size: 1573 3829
BsaBI GATnn'nnATC
Cuts at: 6 2916 4953 6
Size: 2910 2037 455
Fragments arranged by size:
2910 2037 455
BsaHI Gr'CG_yC
Cuts at: 2833 3081 3783 5279 2833
Size: 248 702 1496 2956
Fragments arranged by size:
2956 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2591 3112 3790 4064 4211 4622 2591
Size: 521 678 274 147 411 3371
Fragments arranged by size:
3371 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 2095 2125 2352 2382 508
Size: 30 1557 30 227 30 3528
Fragments arranged by size:
3528 1557 227 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 487 715 1174 1257 1363 1996 2029 2091
Size: 75 228 459 83 106 633 33 62
Cuts at: 2091 2378 412
Size: 287 3436
Fragments arranged by size:
3436 633 459 287 228 106 83 75
62 33
BseYI C'CCAG_C
Cuts at: 1595 2000 2156 2827 3585 4162 4658 1595
Size: 405 156 671 758 577 496 2339
Fragments arranged by size:
2339 758 671 577 496 405 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 621
Size: 5402
BsiEI CG_ry'CG
Cuts at: 104 2944 2990 4198 104
Size: 2840 46 1208 1308
Fragments arranged by size:
2840 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 694 786 872 2105 2275 2416 3194 3384
Size: 263 92 86 1233 170 141 778 190
Cuts at: 3384 4176 431
Size: 792 1657
Fragments arranged by size:
1657 1233 792 778 263 190 170 141
92 86
BsmI GAATG_Cn'
Cuts at: 309 1417 309
Size: 1108 4294
Bsp1286I G_dGCh'C
Cuts at: 431 694 786 872 1134 1944 2105 2275 2416
Size: 263 92 86 262 810 161 170 141
Cuts at: 2416 3027 3120 3194 3384 3446 4176 431
Size: 611 93 74 190 62 730 1657
Fragments arranged by size:
1657 810 730 611 263 262 190 170
161 141 93 92 86 74 62
BspEI T'CCGG_A
Cuts at: 4622 4622
Size: 5402
BspHI T'CATG_A
Cuts at: 4578 4578
Size: 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 570 643 1703 1817 2073 2452 2666 2968 3349
Size: 73 1060 114 256 379 214 302 381
Cuts at: 3349 570
Size: 2623
Fragments arranged by size:
2623 1060 381 379 302 256 214 114
73
BsrBI CCG'CTC
Cuts at: 50 1773 2378 3694 3748 5232 5397 50
Size: 1723 605 1316 54 1484 165 55
Fragments arranged by size:
1723 1484 1316 605 165 55 54
BsrDI GCAATG_nn'
Cuts at: 168 3314 168
Size: 3146 2256
BsrFI r'CCGG_y
Cuts at: 2231 3400 3581 3790 2231
Size: 1169 181 209 3843
Fragments arranged by size:
3843 1169 209 181
BssHII G'CGCG_C
Cuts at: 823 823
Size: 5402
BssSI C'ACGA_G
Cuts at: 430 3673 4031 430
Size: 3243 358 1801
Fragments arranged by size:
3243 1801 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2779 2779
Size: 5402
BstBI TT'CG_AA
Cuts at: 3763 3763
Size: 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 543 543
Size: 5402
BstYI r'GATC_y
Cuts at: 1 1200 1320 1625 2427 2894 2917 3252 3498
Size: 1199 120 305 802 467 23 335 246
Cuts at: 3498 4510 4596 4608 4954 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1199 1012 802 467 449 346 335 305
246 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 730 730
Size: 5402
BtgI C'CryG_G
Cuts at: 932 932
Size: 5402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3352 3533 3696 3352
Size: 181 163 5058
Fragments arranged by size:
5058 181 163
BtsI GCAGTG_nn'
Cuts at: 741 784 2645 741
Size: 43 1861 3498
Fragments arranged by size:
3498 1861 43
DraI TTT'AAA
Cuts at: 2401 2401
Size: 5402
DrdI GACnn_nn'nnGTC
Cuts at: 1394 3108 3966 1394
Size: 1714 858 2830
Fragments arranged by size:
2830 1714 858
EaeI y'GGCC_r
Cuts at: 359 2987 3161 3552 3579 4924 359
Size: 2628 174 391 27 1345 837
Fragments arranged by size:
2628 1345 837 391 174 27
EagI C'GGCC_G
Cuts at: 2987 2987
Size: 5402
EarI CTCTTCn'nnn_
Cuts at: 383 860 1147 3425 3635 383
Size: 477 287 2278 210 2150
Fragments arranged by size:
2278 2150 477 287 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 565 817 2211 3610 3920 4066 5212 150
Size: 415 252 1394 1399 310 146 1146 340
Fragments arranged by size:
1399 1394 1146 415 340 310 252 146
EcoICRI GAG'CTC
Cuts at: 870 2414 870
Size: 1544 3858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 196 340 394 415 478 859 1039 1093
Size: 17 144 54 21 63 381 180 54
Cuts at: 1093 1235 1260 1366 1447 1462 1525 1738 1864
Size: 142 25 106 81 15 63 213 126
Cuts at: 1864 1921 1936 1948 2032 2278 3227 3659 4406
Size: 57 15 12 84 246 949 432 747
Cuts at: 4406 4674 179
Size: 268 907
Fragments arranged by size:
949 907 747 432 381 268 246 213
180 144 142 126 106 84 81 63
63 57 54 54 25 21 17 15
15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 511 511
Size: 5402
EcoO109I rG'GnC_Cy
Cuts at: 2036 2541 2036
Size: 505 4897
EcoRI G'AATT_C
Cuts at: 2406 2406
Size: 5402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 889 1467 3421 889
Size: 578 1954 2870
Fragments arranged by size:
2870 1954 578
FspI TGC'GCA
Cuts at: 1813 3183 1813
Size: 1370 4032
HaeII r_GCGC'y
Cuts at: 354 2836 3084 4106 5085 354
Size: 2482 248 1022 979 671
Fragments arranged by size:
2482 1022 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 279 311 999 1031 2352 2384 3244
Size: 32 12 32 688 32 1321 32 860
Cuts at: 3244 3274 3276 3306 235
Size: 30 2 30 2331
Fragments arranged by size:
2331 1321 860 688 32 32 32 32
30 30 12 2
HincII GTy'rAC
Cuts at: 2441 2441
Size: 5402
HindIII A'AGCT_T
Cuts at: 1217 1217
Size: 5402
KasI G'GCGC_C
Cuts at: 2832 3080 2832
Size: 248 5154
KpnI G_GTAC'C
Cuts at: 2422 2422
Size: 5402
MluI A'CGCG_T
Cuts at: 4835 4835
Size: 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2033 4073 4257 2033
Size: 2040 184 3178
Fragments arranged by size:
3178 2040 184
MscI TGG'CCA
Cuts at: 361 3163 361
Size: 2802 2600
MslI CAynn'nnrTG
Cuts at: 2028 3391 3518 2028
Size: 1363 127 3912
Fragments arranged by size:
3912 1363 127
MspA1I CmG'CkG
Cuts at: 1028 1071 1503 1686 1893 2004 2160 2190 2513
Size: 43 432 183 207 111 156 30 323
Cuts at: 2513 2827 3187 4200 4445 1028
Size: 314 360 1013 245 1985
Fragments arranged by size:
1985 1013 432 360 323 314 245 207
183 156 111 43 30
NaeI GCC'GGC
Cuts at: 2233 3583 2233
Size: 1350 4052
NarI GG'CG_CC
Cuts at: 2833 3081 2833
Size: 248 5154
NcoI C'CATG_G
Cuts at: 932 932
Size: 5402
NgoMIV G'CCGG_C
Cuts at: 2231 3581 2231
Size: 1350 4052
NspI r_CATG'y
Cuts at: 1005 2289 2455 2955 3862 1005
Size: 1284 166 500 907 2545
Fragments arranged by size:
2545 1284 907 500 166
PciI A'CATG_T
Cuts at: 3858 3858
Size: 5402
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 5402
PfoI T'CCnGG_A
Cuts at: 371 1237 2141 371
Size: 866 904 3632
Fragments arranged by size:
3632 904 866
PmeI GTTT'AAAC
Cuts at: 2401 2401
Size: 5402
PmlI CAC'GTG
Cuts at: 1182 1182
Size: 5402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 4541
Size: 32 5370
PpuMI rG'GwC_Cy
Cuts at: 2036 2036
Size: 5402
PshAI GACnn'nnGTC
Cuts at: 254 254
Size: 5402
PspXI vC'TCGA_Gb
Cuts at: 1489 1489
Size: 5402
PstI C_TGCA'G
Cuts at: 683 1700 1763 2449 3134 683
Size: 1017 63 686 685 2951
Fragments arranged by size:
2951 1017 686 685 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5402
PvuII CAG'CTG
Cuts at: 1071 1503 1686 1893 2004 2160 2190 2827 3187
Size: 432 183 207 111 156 30 637 360
Cuts at: 3187 1071
Size: 3286
Fragments arranged by size:
3286 637 432 360 207 183 156 111
30
RsrII CG'GwC_CG
Cuts at: 2214 3597 2214
Size: 1383 4019
SacI G_AGCT'C
Cuts at: 872 2416 872
Size: 1544 3858
SalI G'TCGA_C
Cuts at: 2439 2439
Size: 5402
SanDI GG'GwC_CC
Cuts at: 2036 2036
Size: 5402
SapI GCTCTTCn'nnn_
Cuts at: 383 860 1147 3425 3635 383
Size: 477 287 2278 210 2150
Fragments arranged by size:
2278 2150 477 287 210
SbfI CC_TGCA'GG
Cuts at: 2449 2449
Size: 5402
ScaI AGT'ACT
Cuts at: 4919 4919
Size: 5402
SfcI C'TryA_G
Cuts at: 33 236 679 1696 1759 1960 2445 3130 4123
Size: 203 443 1017 63 201 485 685 993
Cuts at: 4123 4314 33
Size: 191 1121
Fragments arranged by size:
1121 1017 993 685 485 443 203 201
191 63
SfoI GGC'GCC
Cuts at: 2834 3082 2834
Size: 248 5154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
SmaI CCC'GGG
Cuts at: 2424 2424
Size: 5402
SmlI C'TyrA_G
Cuts at: 899 1282 1489 2149 2557 3472 3964 4226 899
Size: 383 207 660 408 915 492 262 2075
Fragments arranged by size:
2075 915 660 492 408 383 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5402
SphI G_CATG'C
Cuts at: 1005 2289 2455 2955 1005
Size: 1284 166 500 3452
Fragments arranged by size:
3452 1284 500 166
StuI AGG'CCT
Cuts at: 1510 1510
Size: 5402
StyI C'CwwG_G
Cuts at: 932 2096 2114 2536 2890 932
Size: 1164 18 422 354 3444
Fragments arranged by size:
3444 1164 422 354 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 959 1112 2985 3118 3577 3786 959
Size: 153 1873 133 459 209 2575
Fragments arranged by size:
2575 1873 459 209 153 133
TatI w'GTAC_w
Cuts at: 4917 4917
Size: 5402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 2095 2127 4919 4951 508
Size: 32 1555 32 2792 32 959
Fragments arranged by size:
2792 1555 959 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3199 3199
Size: 5402
XbaI T'CTAG_A
Cuts at: 2433 2433
Size: 5402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 582 2897 369
Size: 213 2315 2874
Fragments arranged by size:
2874 2315 213
XhoI C'TCGA_G
Cuts at: 1489 1489
Size: 5402
XmaI C'CCGG_G
Cuts at: 2422 2422
Size: 5402
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 5402
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI