Restriction map for
pF1KE2463
(Circular) (Six-base) MAPSORT of: pF1KE2463.seq Check: 7572 from: 1 to: 3752
pF1KE2463 3752 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 132
Size: 3752
AccI GT'mk_AC
Cuts at: 209 790 209
Size: 581 3171
Acc65I G'GTAC_C
Cuts at: 522 768 522
Size: 246 3506
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 334 1577 2009 2756 3024 138
Size: 196 1243 432 747 268 866
Fragments arranged by size:
1243 866 747 432 268 196
AflIII A'CryG_T
Cuts at: 692 1104 2208 3185 692
Size: 412 1104 977 1259
Fragments arranged by size:
1259 1104 977 412
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 720 1057 2624 720
Size: 337 1567 1848
Fragments arranged by size:
1848 1567 337
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 772 772
Size: 3752
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 522 768 1182 1430 1465 522
Size: 246 414 248 35 2809
Fragments arranged by size:
2809 414 248 246 35
BanII G_rGCy'C
Cuts at: 134 766 1796 134
Size: 632 1030 2090
Fragments arranged by size:
2090 1030 632
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 431 1272 431
Size: 841 2911
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 1377 1470 2526 1377
Size: 93 1056 2603
Fragments arranged by size:
2603 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 428 2119 426
Size: 2 1691 2059
Fragments arranged by size:
2059 1691 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 292
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 928 1807 2299 2597 574
Size: 354 879 492 298 1729
Fragments arranged by size:
1729 879 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 117 720 1177 1935 2512 3008 117
Size: 603 457 758 577 496 861
Fragments arranged by size:
861 758 603 577 496 457
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 134 766 1377 1470 1544 1734 1796 2526 134
Size: 632 611 93 74 190 62 730 1360
Fragments arranged by size:
1360 730 632 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 802 1016 1318 1699 132
Size: 670 214 302 381 2185
Fragments arranged by size:
2185 670 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958
Size: 776 467 23 335 246 1012 86 12
Cuts at: 2958 3304 1
Size: 346 449
Fragments arranged by size:
1012 776 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 210 210
Size: 3752
BtgI C'CryG_G
Cuts at: 107 107
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 162 496 1702 1883 2046 162
Size: 334 1206 181 163 1868
Fragments arranged by size:
1868 1206 334 181 163
BtsI GCAGTG_nn'
Cuts at: 128 995 128
Size: 867 2885
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 642 697 1458 2316 642
Size: 55 761 858 2078
Fragments arranged by size:
2078 858 761 55
EaeI y'GGCC_r
Cuts at: 110 1337 1511 1902 1929 3274 110
Size: 1227 174 391 27 1345 588
Fragments arranged by size:
1345 1227 588 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 292 334 1577 2009 2756 3024 138
Size: 154 42 1243 432 747 268 866
Fragments arranged by size:
1243 866 747 432 268 154 42
EcoO109I rG'GnC_Cy
Cuts at: 891 891
Size: 3752
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 670 1533 670
Size: 863 2889
FspAI rTGC'GCAy
Cuts at: 670 670
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 495 527 1594 1624 1626 1656 495
Size: 32 1067 30 2 30 2591
Fragments arranged by size:
2591 1067 32 30 30 2
HincII GTy'rAC
Cuts at: 690 791 690
Size: 101 3651
HindIII A'AGCT_T
Cuts at: 184 184
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 526 772 526
Size: 246 3506
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 370 1741 1868 370
Size: 1371 127 2254
Fragments arranged by size:
2254 1371 127
MspA1I CmG'CkG
Cuts at: 863 1177 1537 2550 2795 863
Size: 314 360 1013 245 1820
Fragments arranged by size:
1820 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 696 805 1305 2212 696
Size: 109 500 907 2236
Fragments arranged by size:
2236 907 500 109
PciI A'CATG_T
Cuts at: 692 2208 692
Size: 1516 2236
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 589 907 1822 2314 2576 589
Size: 318 915 492 262 1765
Fragments arranged by size:
1765 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StuI AGG'CCT
Cuts at: 195 195
Size: 3752
StyI C'CwwG_G
Cuts at: 107 471 886 1240 107
Size: 364 415 354 2619
Fragments arranged by size:
2619 415 364 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 1335 1468 1927 2136 643
Size: 692 133 459 209 2259
Fragments arranged by size:
2259 692 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 650 682 3269 3301 650
Size: 32 2587 32 1101
Fragments arranged by size:
2587 1101 32 32
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI