Restriction map for
pF1KE2462
(Circular) (Six-base) MAPSORT of: pF1KE2462.seq Check: 4323 from: 1 to: 3467
pF1KE2462 3467 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 341 1292 1724 2471 2739 226
Size: 115 951 432 747 268 954
Fragments arranged by size:
954 951 747 432 268 115
AflIII A'CryG_T
Cuts at: 819 1923 2900 819
Size: 1104 977 1386
Fragments arranged by size:
1386 1104 977
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AleI CACnn'nnGTG
Cuts at: 322 322
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 138 141 772 2339 138
Size: 3 631 1567 1266
Fragments arranged by size:
1567 1266 631 3
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 268 471 2820 3076 268
Size: 203 2349 256 659
Fragments arranged by size:
2349 659 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 487 487
Size: 3467
BamHI G'GATC_C
Cuts at: 298 492 298
Size: 194 3273
BanI G'GyrC_C
Cuts at: 483 897 1145 1180 483
Size: 414 248 35 2770
Fragments arranged by size:
2770 414 248 35
BanII G_rGCy'C
Cuts at: 481 1511 481
Size: 1030 2437
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 987 987
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 1092 1185 2241 1092
Size: 93 1056 2318
Fragments arranged by size:
2318 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 679 760 1095 3239 679
Size: 81 335 2144 907
Fragments arranged by size:
2144 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3467
Bpu10I CC'TnA_GC
Cuts at: 227 227
Size: 3467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1522 2014 2312 643
Size: 879 492 298 1798
Fragments arranged by size:
1798 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 3171 328
Size: 2843 624
BsaAI yAC'GTr
Cuts at: 820 820
Size: 3467
BsaBI GATnn'nnATC
Cuts at: 6 352 981 3018 6
Size: 346 629 2037 455
Fragments arranged by size:
2037 629 455 346
BsaHI Gr'CG_yC
Cuts at: 898 1146 1848 3344 898
Size: 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 197 656 1177 1855 2129 2276 2687 197
Size: 459 521 678 274 147 411 977
Fragments arranged by size:
977 678 521 459 411 274 147
BseYI C'CCAG_C
Cuts at: 892 1650 2227 2723 892
Size: 758 577 496 1636
Fragments arranged by size:
1636 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 481 1092 1185 1259 1449 1511 2241 481
Size: 611 93 74 190 62 730 1707
Fragments arranged by size:
1707 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 2643 2643
Size: 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 731 1033 1414 517
Size: 214 302 381 2570
Fragments arranged by size:
2570 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 141 844 141
Size: 703 2764
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstYI r'GATC_y
Cuts at: 1 298 492 959 982 1317 1563 2575 2661
Size: 297 194 467 23 335 246 1012 86
Cuts at: 2661 2673 3019 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 297 246 194
86 23 12
BtgI C'CryG_G
Cuts at: 107 303 107
Size: 196 3271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 710 710
Size: 3467
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 427 1052 1226 1617 1644 2989 427
Size: 625 174 391 27 1345 905
Fragments arranged by size:
1345 905 625 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 341 343 1292 1724 2471 2739 226
Size: 115 2 949 432 747 268 954
Fragments arranged by size:
954 949 747 432 268 115 2
EcoO109I rG'GnC_Cy
Cuts at: 606 606
Size: 3467
EcoRI G'AATT_C
Cuts at: 268 471 268
Size: 203 3264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 901 1149 2171 3150 901
Size: 248 1022 979 1218
Fragments arranged by size:
1218 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 1309 1339 1341 1371 157
Size: 32 1120 30 2 30 2253
Fragments arranged by size:
2253 1120 32 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 322 1456 1583 322
Size: 1134 127 2206
Fragments arranged by size:
2206 1134 127
MspA1I CmG'CkG
Cuts at: 138 578 892 1252 2265 2510 138
Size: 440 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 440 360 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NcoI C'CATG_G
Cuts at: 107 107
Size: 3467
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PstI C_TGCA'G
Cuts at: 146 257 514 1199 146
Size: 111 257 685 2414
Fragments arranged by size:
2414 685 257 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 138 892 1252 138
Size: 754 360 2353
Fragments arranged by size:
2353 754 360
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 142 253 510 1195 2188 2379 33
Size: 109 111 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 257 191 111 109
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 622 1537 2029 2291 622
Size: 915 492 262 1798
Fragments arranged by size:
1798 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 107 278 601 955 107
Size: 171 323 354 2619
Fragments arranged by size:
2619 354 323 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 2982 2982
Size: 3467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3435
Tth111I GACn'n_nGTC
Cuts at: 1264 1264
Size: 3467
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
XmnI GAAnn'nnTTC
Cuts at: 268 268
Size: 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI