Restriction map for
pF1KE2456
(Circular) (Six-base) MAPSORT of: pF1KE2456.seq Check: 1622 from: 1 to: 8240
pF1KE2456 8240 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 3684 3820 4501 5278 3684
Size: 136 681 777 6646
Fragments arranged by size:
6646 777 681 136
Acc65I G'GTAC_C
Cuts at: 5256 5256
Size: 8240
AclI AA'CG_TT
Cuts at: 8080 8080
Size: 8240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 626 1525 2767 3047 3068 3644 4169 4244
Size: 453 899 1242 280 21 576 525 75
Cuts at: 4244 4337 4346 6065 6497 7244 7512 173
Size: 93 9 1719 432 747 268 901
Fragments arranged by size:
1719 1242 901 899 747 576 525 453
432 280 268 93 75 21 9
AflIII A'CryG_T
Cuts at: 5592 6696 7673 5592
Size: 1104 977 6159
Fragments arranged by size:
6159 1104 977
AgeI A'CCGG_T
Cuts at: 5036 6628 5036
Size: 1592 6648
AhdI GACnn_n'nnGTC
Cuts at: 5037 5037
Size: 8240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4037 4037
Size: 8240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 878
Size: 32 8208
AlwNI CAG_nnn'CTG
Cuts at: 196 298 604 646 862 1318 2962 5545 7112
Size: 102 306 42 216 456 1644 2583 1567
Cuts at: 7112 196
Size: 1324
Fragments arranged by size:
2583 1644 1567 1324 456 306 216 102
42
ApaLI G'TGCA_C
Cuts at: 7010 7010
Size: 8240
ApoI r'AATT_y
Cuts at: 916 1751 1861 1889 2049 2422 3222 3305 3526
Size: 835 110 28 160 373 800 83 221
Cuts at: 3526 4567 4959 5244 7593 7849 916
Size: 1041 392 285 2349 256 1307
Fragments arranged by size:
2349 1307 1041 835 800 392 373 285
256 221 160 110 83 28
AseI AT'TA_AT
Cuts at: 20 2497 2720 3769 20
Size: 2477 223 1049 4491
Fragments arranged by size:
4491 2477 1049 223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8240
AvaI C'yCGr_G
Cuts at: 314 3464 3563 5260 314
Size: 3150 99 1697 3294
Fragments arranged by size:
3294 3150 1697 99
AvrII C'CTAG_G
Cuts at: 5131 5131
Size: 8240
BamHI G'GATC_C
Cuts at: 3942 5265 3942
Size: 1323 6917
BanI G'GyrC_C
Cuts at: 5256 5670 5918 5953 5256
Size: 414 248 35 7543
Fragments arranged by size:
7543 414 248 35
BanII G_rGCy'C
Cuts at: 1389 2066 2802 3068 5254 6284 1389
Size: 677 736 266 2186 1030 3345
Fragments arranged by size:
3345 2186 1030 736 677 266
BbeI G_GCGC'C
Cuts at: 5674 5922 5674
Size: 248 7992
BbsI GAAGACnn'nnnn_
Cuts at: 2947 3270 3453 4348 2947
Size: 323 183 895 6839
Fragments arranged by size:
6839 895 323 183
BbvCI CC'TCA_GC
Cuts at: 2317 2663 2317
Size: 346 7894
BciVI GTATCCnnnnn_n'
Cuts at: 6138 6899 8240 6138
Size: 761 1341 6138
Fragments arranged by size:
6138 1341 761
BclI T'GATC_A
Cuts at: 4641 4770 4932 4995 5760 4641
Size: 129 162 63 765 7121
Fragments arranged by size:
7121 765 162 129 63
BfrBI ATG'CAT
Cuts at: 1415 1415
Size: 8240
BglII A'GATC_T
Cuts at: 400 694 4053 4984 5152 5167 5755 7792 400
Size: 294 3359 931 168 15 588 2037 848
Fragments arranged by size:
3359 2037 931 848 588 294 168 15
BlpI GC'TnA_GC
Cuts at: 5352 5352
Size: 8240
Bme1580I G_kGCm'C
Cuts at: 5865 5958 7014 5865
Size: 93 1056 7091
Fragments arranged by size:
7091 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2945 3153 3619 3873 5452 5533 5868 8012 2945
Size: 208 466 254 1579 81 335 2144 3173
Fragments arranged by size:
3173 2144 1579 466 335 254 208 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 501 6607 501
Size: 6106 2134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 2726 4259 5148 5238 1193
Size: 1533 1533 889 90 4195
Fragments arranged by size:
4195 1533 1533 889 90
Bpu10I CC'TnA_GC
Cuts at: 2317 2663 2317
Size: 346 7894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1105 2197 5416 6295 6787 7085 397
Size: 708 1092 3219 879 492 298 1552
Fragments arranged by size:
3219 1552 1092 879 708 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4466 7944 4466
Size: 3478 4762
BsaAI yAC'GTr
Cuts at: 4668 5593 4668
Size: 925 7315
BsaBI GATnn'nnATC
Cuts at: 6 276 576 2765 4871 5754 7791 6
Size: 270 300 2189 2106 883 2037 455
Fragments arranged by size:
2189 2106 2037 883 455 300 270
BsaHI Gr'CG_yC
Cuts at: 5671 5919 6621 8117 5671
Size: 248 702 1496 5794
Fragments arranged by size:
5794 1496 702 248
BsaWI w'CCGG_w
Cuts at: 268 5036 5205 5429 5950 6628 6902 7049 7460
Size: 4768 169 224 521 678 274 147 411
Cuts at: 7460 268
Size: 1048
Fragments arranged by size:
4768 1048 678 521 411 274 224 169
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 878 908 2022 2052 3360 3390 3605
Size: 30 598 30 1114 30 1308 30 215
Cuts at: 3605 3635 3716 3746 250
Size: 30 81 30 4744
Fragments arranged by size:
4744 1308 1114 598 215 81 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 2030 4113 4166 772
Size: 1258 2083 53 4846
Fragments arranged by size:
4846 2083 1258 53
BseYI C'CCAG_C
Cuts at: 1373 3923 5665 6423 7000 7496 1373
Size: 2550 1742 758 577 496 2117
Fragments arranged by size:
2550 2117 1742 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1389 4043 1389
Size: 2654 5586
BsiEI CG_ry'CG
Cuts at: 104 5782 5828 7036 104
Size: 5678 46 1208 1308
Fragments arranged by size:
5678 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1389 2066 5254 6032 6222 7014 1389
Size: 677 3188 778 190 792 2615
Fragments arranged by size:
3188 2615 792 778 677 190
BsmI GAATG_Cn'
Cuts at: 1782 1909 3741 1782
Size: 127 1832 6281
Fragments arranged by size:
6281 1832 127
BsmBI CGTCTCn'nnnn_
Cuts at: 2026 2026
Size: 8240
Bsp1286I G_dGCh'C
Cuts at: 1389 2066 2802 3068 5254 5865 5958 6032 6222
Size: 677 736 266 2186 611 93 74 190
Cuts at: 6222 6284 7014 1389
Size: 62 730 2615
Fragments arranged by size:
2615 2186 736 730 677 611 266 190
93 74 62
BspEI T'CCGG_A
Cuts at: 268 7460 268
Size: 7192 1048
BspHI T'CATG_A
Cuts at: 1680 1827 3111 7416 1680
Size: 147 1284 4305 2504
Fragments arranged by size:
4305 2504 1284 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5290 5504 5806 6187 5290
Size: 214 302 381 7343
Fragments arranged by size:
7343 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1722 6532 6586 8070 8235 50
Size: 1672 4810 54 1484 165 55
Fragments arranged by size:
4810 1672 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2388 4136 4531 6152 2388
Size: 1748 395 1621 4476
Fragments arranged by size:
4476 1748 1621 395
BsrFI r'CCGG_y
Cuts at: 5036 6238 6419 6628 5036
Size: 1202 181 209 6648
Fragments arranged by size:
6648 1202 209 181
BssSI C'ACGA_G
Cuts at: 6511 6869 6511
Size: 358 7882
BstAPI GCAn_nnn'nTGC
Cuts at: 5617 5617
Size: 8240
BstBI TT'CG_AA
Cuts at: 6601 6601
Size: 8240
BstEII G'GTnAC_C
Cuts at: 1009 5135 1009
Size: 4126 4114
BstXI CCAn_nnnn'nTGG
Cuts at: 2809 2809
Size: 8240
BstYI r'GATC_y
Cuts at: 1 199 271 400 571 694 3397 3942 4053
Size: 198 72 129 171 123 2703 545 111
Cuts at: 4053 4246 4984 5152 5167 5265 5732 5755 6090
Size: 193 738 168 15 98 467 23 335
Cuts at: 6090 6336 7348 7434 7446 7792 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2703 1012 738 545 467 449 346 335
246 198 193 171 168 129 123 111
98 86 72 23 15 12
BstZ17I GTA'TAC
Cuts at: 3821 4502 3821
Size: 681 7559
Bsu36I CC'TnA_GG
Cuts at: 905 2343 905
Size: 1438 6802
BtgI C'CryG_G
Cuts at: 3451 3451
Size: 8240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6190 6371 6534 6190
Size: 181 163 7896
Fragments arranged by size:
7896 181 163
BtsI GCAGTG_nn'
Cuts at: 261 498 5130 5483 261
Size: 237 4632 353 3018
Fragments arranged by size:
4632 3018 353 237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1081 1116 1081
Size: 35 8205
DraI TTT'AAA
Cuts at: 1461 1574 2007 3014 4976 5239 1461
Size: 113 433 1007 1962 263 4462
Fragments arranged by size:
4462 1962 1007 433 263 113
DrdI GACnn_nn'nnGTC
Cuts at: 5946 6804 5946
Size: 858 7382
EaeI y'GGCC_r
Cuts at: 389 5825 5999 6390 6417 7762 389
Size: 5436 174 391 27 1345 867
Fragments arranged by size:
5436 1345 867 391 174 27
EagI C'GGCC_G
Cuts at: 5825 5825
Size: 8240
EarI CTCTTCn'nnn_
Cuts at: 470 605 905 2228 2990 3091 4322 6263 6473
Size: 135 300 1323 762 101 1231 1941 210
Cuts at: 6473 470
Size: 2237
Fragments arranged by size:
2237 1941 1323 1231 762 300 210 135
101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6448 6758 6904 8050 6448
Size: 310 146 1146 6638
Fragments arranged by size:
6638 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1387 2064 5252 1387
Size: 677 3188 4375
Fragments arranged by size:
4375 3188 677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 626 1193 1525 2726 2767 3047 3068 3644
Size: 453 567 332 1201 41 280 21 576
Cuts at: 3644 4169 4244 4259 4337 4346 5148 5238 6065
Size: 525 75 15 78 9 802 90 827
Cuts at: 6065 6497 7244 7512 173
Size: 432 747 268 901
Fragments arranged by size:
1201 901 827 802 747 576 567 525
453 432 332 280 268 90 78 75
41 21 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1743 2167 4412 1743
Size: 424 2245 5571
Fragments arranged by size:
5571 2245 424
EcoO109I rG'GnC_Cy
Cuts at: 1350 3127 5379 1350
Size: 1777 2252 4211
Fragments arranged by size:
4211 2252 1777
EcoRI G'AATT_C
Cuts at: 2049 2422 5244 2049
Size: 373 2822 5045
Fragments arranged by size:
5045 2822 373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 4090 6259 2890
Size: 1200 2169 4871
Fragments arranged by size:
4871 2169 1200
FspI TGC'GCA
Cuts at: 6021 6021
Size: 8240
HaeII r_GCGC'y
Cuts at: 5674 5922 6944 7923 5674
Size: 248 1022 979 5991
Fragments arranged by size:
5991 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 250 256 282 286 288 318 392
Size: 32 52 6 26 4 2 30 74
Cuts at: 392 424 496 528 586 618 1809 1827 1841
Size: 32 72 32 58 32 1191 18 14
Cuts at: 1841 1859 1993 2025 2053 2085 3400 3432 3447
Size: 18 134 32 28 32 1315 32 15
Cuts at: 3447 3479 4463 4495 4662 4694 4932 4939 4964
Size: 32 984 32 167 32 238 7 25
Cuts at: 4964 4971 5023 5055 5137 5169 6082 6112 6114
Size: 7 52 32 82 32 913 30 2
Cuts at: 6114 6144 166
Size: 30 2262
Fragments arranged by size:
2262 1315 1191 984 913 238 167 134
82 74 72 58 52 52 32 32
32 32 32 32 32 32 32 32
32 32 30 30 30 28 26 25
18 18 15 14 7 7 6 4
2 2
HincII GTy'rAC
Cuts at: 1877 5279 1877
Size: 3402 4838
HpaI GTT'AAC
Cuts at: 1877 1877
Size: 8240
KasI G'GCGC_C
Cuts at: 5670 5918 5670
Size: 248 7992
KpnI G_GTAC'C
Cuts at: 5260 5260
Size: 8240
MfeI C'AATT_G
Cuts at: 3557 3557
Size: 8240
MluI A'CGCG_T
Cuts at: 7673 7673
Size: 8240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 146 928 3188 3730 5206 6911 7095 146
Size: 782 2260 542 1476 1705 184 1291
Fragments arranged by size:
2260 1705 1476 1291 782 542 184
MscI TGG'CCA
Cuts at: 6001 6001
Size: 8240
MslI CAynn'nnrTG
Cuts at: 122 542 2362 2657 3116 6229 6356 122
Size: 420 1820 295 459 3113 127 2006
Fragments arranged by size:
3113 2006 1820 459 420 295 127
MspA1I CmG'CkG
Cuts at: 1318 1373 5351 5665 6025 7038 7283 1318
Size: 55 3978 314 360 1013 245 2275
Fragments arranged by size:
3978 2275 1013 360 314 245 55
NaeI GCC'GGC
Cuts at: 6421 6421
Size: 8240
NarI GG'CG_CC
Cuts at: 5671 5919 5671
Size: 248 7992
NgoMIV G'CCGG_C
Cuts at: 6419 6419
Size: 8240
NsiI A_TGCA'T
Cuts at: 1417 1417
Size: 8240
NspI r_CATG'y
Cuts at: 1415 4606 5293 5793 6700 1415
Size: 3191 687 500 907 2955
Fragments arranged by size:
3191 2955 907 687 500
PacI TTA_AT'TAA
Cuts at: 1067 1067
Size: 8240
PciI A'CATG_T
Cuts at: 6696 6696
Size: 8240
PflMI CCAn_nnn'nTGG
Cuts at: 3075 3075
Size: 8240
PfoI T'CCnGG_A
Cuts at: 4819 4819
Size: 8240
PmeI GTTT'AAAC
Cuts at: 5239 5239
Size: 8240
PmlI CAC'GTG
Cuts at: 4668 4668
Size: 8240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 878 910 7379 7411 878
Size: 32 6469 32 1707
Fragments arranged by size:
6469 1707 32 32
PpuMI rG'GwC_Cy
Cuts at: 3127 3127
Size: 8240
PshAI GACnn'nnGTC
Cuts at: 3953 3953
Size: 8240
PsiI TTA'TAA
Cuts at: 1163 1229 1163
Size: 66 8174
PstI C_TGCA'G
Cuts at: 5287 5972 5287
Size: 685 7555
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8240
PvuII CAG'CTG
Cuts at: 1318 1373 5665 6025 1318
Size: 55 4292 360 3533
Fragments arranged by size:
4292 3533 360 55
RsrII CG'GwC_CG
Cuts at: 6435 6435
Size: 8240
SacI G_AGCT'C
Cuts at: 1389 2066 5254 1389
Size: 677 3188 4375
Fragments arranged by size:
4375 3188 677
SalI G'TCGA_C
Cuts at: 5277 5277
Size: 8240
SapI GCTCTTCn'nnn_
Cuts at: 4322 6263 6473 4322
Size: 1941 210 6089
Fragments arranged by size:
6089 1941 210
SbfI CC_TGCA'GG
Cuts at: 5287 5287
Size: 8240
ScaI AGT'ACT
Cuts at: 2948 7757 2948
Size: 4809 3431
SexAI A'CCwGG_T
Cuts at: 3123 3123
Size: 8240
SfcI C'TryA_G
Cuts at: 33 2044 3137 5283 5968 6961 7152 33
Size: 2011 1093 2146 685 993 191 1121
Fragments arranged by size:
2146 2011 1121 1093 993 685 191
SfoI GGC'GCC
Cuts at: 5672 5920 5672
Size: 248 7992
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8240
SmaI CCC'GGG
Cuts at: 5262 5262
Size: 8240
SmlI C'TyrA_G
Cuts at: 412 1120 2176 3563 5395 6310 6802 7064 412
Size: 708 1056 1387 1832 915 492 262 1588
Fragments arranged by size:
1832 1588 1387 1056 915 708 492 262
SpeI A'CTAG_T
Cuts at: 67 3744 67
Size: 3677 4563
SphI G_CATG'C
Cuts at: 1415 4606 5293 5793 1415
Size: 3191 687 500 3862
Fragments arranged by size:
3862 3191 687 500
SspI AAT'ATT
Cuts at: 1225 2281 2575 2832 3474 1225
Size: 1056 294 257 642 5991
Fragments arranged by size:
5991 1056 642 294 257
StuI AGG'CCT
Cuts at: 2443 4374 4407 4418 2443
Size: 1931 33 11 6265
Fragments arranged by size:
6265 1931 33 11
StyI C'CwwG_G
Cuts at: 1489 1635 1914 2231 2330 5131 5374 5728 1489
Size: 146 279 317 99 2801 243 354 4001
Fragments arranged by size:
4001 2801 354 317 279 243 146 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4799 5823 5956 6415 6624 4799
Size: 1024 133 459 209 6415
Fragments arranged by size:
6415 1024 459 209 133
TatI w'GTAC_w
Cuts at: 2946 3726 5200 7755 2946
Size: 780 1474 2555 3431
Fragments arranged by size:
3431 2555 1474 780
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 3716 3748 5101 5133 7757 7789 2022
Size: 32 1662 32 1353 32 2624 32 2473
Fragments arranged by size:
2624 2473 1662 1353 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6037 6037
Size: 8240
XbaI T'CTAG_A
Cuts at: 2646 5271 2646
Size: 2625 5615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5735 5735
Size: 8240
XhoI C'TCGA_G
Cuts at: 3563 3563
Size: 8240
XmaI C'CCGG_G
Cuts at: 5260 5260
Size: 8240
XmnI GAAnn'nnTTC
Cuts at: 2870 3245 4304 4345 2870
Size: 375 1059 41 6765
Fragments arranged by size:
6765 1059 375 41
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI ApaI AscI BaeI
BcgI BglI BmgBI BmtI BsiWI BsrGI BssHII ClaI
DraIII EcoRV FseI FspAI HindIII NcoI NdeI NheI
NotI NruI PasI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI