Restriction map for
pF1KE2454
(Circular) (Six-base) MAPSORT of: pF1KE2454.seq Check: 8463 from: 1 to: 5651
pF1KE2454 5651 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 2689 2689
Size: 5651
Acc65I G'GTAC_C
Cuts at: 2667 2667
Size: 5651
AclI AA'CG_TT
Cuts at: 5491 5491
Size: 5651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1741 1897 1967 3476 3908 4655 4923 1741
Size: 156 70 1509 432 747 268 2469
Fragments arranged by size:
2469 1509 747 432 268 156 70
AflIII A'CryG_T
Cuts at: 1562 3003 4107 5084 1562
Size: 1441 1104 977 2129
Fragments arranged by size:
2129 1441 1104 977
AgeI A'CCGG_T
Cuts at: 4039 4039
Size: 5651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 328
Size: 5651
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1033 1065 1673 1705 1033
Size: 32 608 32 4979
Fragments arranged by size:
4979 608 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1864 2956 4523 1864
Size: 1092 1567 2992
Fragments arranged by size:
2992 1567 1092
ApaI G_GGCC'C
Cuts at: 636 636
Size: 5651
ApaLI G'TGCA_C
Cuts at: 130 1885 4421 130
Size: 1755 2536 1360
Fragments arranged by size:
2536 1755 1360
ApoI r'AATT_y
Cuts at: 1026 1055 1138 2497 2521 2655 5004 5260 1026
Size: 29 83 1359 24 134 2349 256 1417
Fragments arranged by size:
2349 1417 1359 256 134 83 29 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 5651
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
AvaI C'yCGr_G
Cuts at: 206 635 656 689 2671 206
Size: 429 21 33 1982 3186
Fragments arranged by size:
3186 1982 429 33 21
BamHI G'GATC_C
Cuts at: 2676 2676
Size: 5651
BanI G'GyrC_C
Cuts at: 261 477 2667 3081 3329 3364 261
Size: 216 2190 414 248 35 2548
Fragments arranged by size:
2548 2190 414 248 216 35
BanII G_rGCy'C
Cuts at: 127 636 1784 1818 2665 3695 127
Size: 509 1148 34 847 1030 2083
Fragments arranged by size:
2083 1148 1030 847 509 34
BbeI G_GCGC'C
Cuts at: 3085 3333 3085
Size: 248 5403
BbsI GAAGACnn'nnnn_
Cuts at: 848 1808 848
Size: 960 4691
BbvCI CC'TCA_GC
Cuts at: 450 450
Size: 5651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 111 145 314 348 111
Size: 34 169 34 5414
Fragments arranged by size:
5414 169 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1026 3549 4310 5651 1026
Size: 2523 761 1341 1026
Fragments arranged by size:
2523 1341 1026 761
BclI T'GATC_A
Cuts at: 1905 3171 1905
Size: 1266 4385
BglI GCCn_nnn'nGGC
Cuts at: 640 649 640
Size: 9 5642
BglII A'GATC_T
Cuts at: 3166 5203 3166
Size: 2037 3614
BlpI GC'TnA_GC
Cuts at: 518 2763 518
Size: 2245 3406
Bme1580I G_kGCm'C
Cuts at: 134 636 1889 2563 3276 3369 4425 134
Size: 502 1253 674 713 93 1056 1360
Fragments arranged by size:
1360 1253 1056 713 674 502 93
BmrI ACTGGGnnnn_n'
Cuts at: 2001 2863 2944 3279 5423 2001
Size: 862 81 335 2144 2229
Fragments arranged by size:
2229 2144 862 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4018 4018
Size: 5651
Bpu10I CC'TnA_GC
Cuts at: 450 948 1448 450
Size: 498 500 4653
Fragments arranged by size:
4653 500 498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2827 3706 4198 4496 2827
Size: 879 492 298 3982
Fragments arranged by size:
3982 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 628 5355 628
Size: 4727 924
BsaAI yAC'GTr
Cuts at: 1305 3004 1305
Size: 1699 3952
BsaBI GATnn'nnATC
Cuts at: 6 3165 5202 6
Size: 3159 2037 455
Fragments arranged by size:
3159 2037 455
BsaHI Gr'CG_yC
Cuts at: 3082 3330 4032 5528 3082
Size: 248 702 1496 3205
Fragments arranged by size:
3205 1496 702 248
BsaWI w'CCGG_w
Cuts at: 242 2840 3361 4039 4313 4460 4871 242
Size: 2598 521 678 274 147 411 1022
Fragments arranged by size:
2598 1022 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 606 636 1198 1228 606
Size: 30 562 30 5029
Fragments arranged by size:
5029 562 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 265 438 523 624 627 676 2064 2209
Size: 73 173 85 101 3 49 1388 145
Cuts at: 2209 192
Size: 3634
Fragments arranged by size:
3634 1388 173 145 101 85 73 49
3
BseYI C'CCAG_C
Cuts at: 3076 3834 4411 4907 3076
Size: 758 577 496 3820
Fragments arranged by size:
3820 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 496
Size: 5651
BsiEI CG_ry'CG
Cuts at: 104 440 3193 3239 4447 104
Size: 336 2753 46 1208 1308
Fragments arranged by size:
2753 1308 1208 336 46
BsiHKAI G_wGCw'C
Cuts at: 134 1166 1818 1889 2665 3443 3633 4425 134
Size: 1032 652 71 776 778 190 792 1360
Fragments arranged by size:
1360 1032 792 778 776 652 190 71
BsmI GAATG_Cn'
Cuts at: 1979 2060 1979
Size: 81 5570
BsmBI CGTCTCn'nnnn_
Cuts at: 171 171
Size: 5651
Bsp1286I G_dGCh'C
Cuts at: 127 134 636 1166 1784 1818 1889 2563 2665
Size: 7 502 530 618 34 71 674 102
Cuts at: 2665 3276 3369 3443 3633 3695 4425 127
Size: 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 674 618 611 530 502 190
102 93 74 71 62 34 7
BspEI T'CCGG_A
Cuts at: 242 4871 242
Size: 4629 1022
BspHI T'CATG_A
Cuts at: 2223 4827 2223
Size: 2604 3047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2701 2915 3217 3598 2701
Size: 214 302 381 4754
Fragments arranged by size:
4754 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3943 3997 5481 5646 50
Size: 3893 54 1484 165 55
Fragments arranged by size:
3893 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3563 3563
Size: 5651
BsrFI r'CCGG_y
Cuts at: 321 474 643 3649 3830 4039 321
Size: 153 169 3006 181 209 1933
Fragments arranged by size:
3006 1933 209 181 169 153
BsrGI T'GTAC_A
Cuts at: 1591 1591
Size: 5651
BssSI C'ACGA_G
Cuts at: 1100 3922 4280 1100
Size: 2822 358 2471
Fragments arranged by size:
2822 2471 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3028 3028
Size: 5651
BstBI TT'CG_AA
Cuts at: 4012 4012
Size: 5651
BstXI CCAn_nnnn'nTGG
Cuts at: 1174 1174
Size: 5651
BstYI r'GATC_y
Cuts at: 1 2676 3143 3166 3501 3747 4759 4845 4857
Size: 2675 467 23 335 246 1012 86 12
Cuts at: 4857 5203 1
Size: 346 449
Fragments arranged by size:
2675 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 587 587
Size: 5651
BtgI C'CryG_G
Cuts at: 107 146 265 1600 107
Size: 39 119 1335 4158
Fragments arranged by size:
4158 1335 119 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3601 3782 3945 3601
Size: 181 163 5307
Fragments arranged by size:
5307 181 163
BtsI GCAGTG_nn'
Cuts at: 1594 2894 1594
Size: 1300 4351
DraI TTT'AAA
Cuts at: 1143 1586 2546 2650 1143
Size: 443 960 104 4144
Fragments arranged by size:
4144 960 443 104
DrdI GACnn_nn'nnGTC
Cuts at: 3357 4215 3357
Size: 858 4793
EaeI y'GGCC_r
Cuts at: 437 3236 3410 3801 3828 5173 437
Size: 2799 174 391 27 1345 915
Fragments arranged by size:
2799 1345 915 391 174 27
EagI C'GGCC_G
Cuts at: 437 3236 437
Size: 2799 2852
EarI CTCTTCn'nnn_
Cuts at: 408 1247 1565 3674 3884 408
Size: 839 318 2109 210 2175
Fragments arranged by size:
2175 2109 839 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 219 3859 4169 4315 5461 127
Size: 92 3640 310 146 1146 317
Fragments arranged by size:
3640 1146 317 310 146 92
EcoICRI GAG'CTC
Cuts at: 1816 2663 1816
Size: 847 4804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1741 1897 1967 3476 3908 4655 4923 1741
Size: 156 70 1509 432 747 268 2469
Fragments arranged by size:
2469 1509 747 432 268 156 70
EcoNI CCTnn'n_nnAGG
Cuts at: 1175 1175
Size: 5651
EcoO109I rG'GnC_Cy
Cuts at: 210 632 650 2790 210
Size: 422 18 2140 3071
Fragments arranged by size:
3071 2140 422 18
EcoRI G'AATT_C
Cuts at: 2655 2655
Size: 5651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3670 3670
Size: 5651
FspI TGC'GCA
Cuts at: 3432 3432
Size: 5651
HaeII r_GCGC'y
Cuts at: 3085 3333 4355 5334 3085
Size: 248 1022 979 3402
Fragments arranged by size:
3402 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 606 638 683 715 1800 1832 2236 2268 3493
Size: 32 45 32 1085 32 404 32 1225
Cuts at: 3493 3523 3525 3555 606
Size: 30 2 30 2702
Fragments arranged by size:
2702 1225 1085 404 45 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2690 2690
Size: 5651
HindIII A'AGCT_T
Cuts at: 1506 1506
Size: 5651
KasI G'GCGC_C
Cuts at: 3081 3329 3081
Size: 248 5403
KpnI G_GTAC'C
Cuts at: 2671 2671
Size: 5651
MluI A'CGCG_T
Cuts at: 5084 5084
Size: 5651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1547 1848 4322 4506 1106
Size: 441 301 2474 184 2251
Fragments arranged by size:
2474 2251 441 301 184
MscI TGG'CCA
Cuts at: 3412 3412
Size: 5651
MslI CAynn'nnrTG
Cuts at: 1599 3640 3767 1599
Size: 2041 127 3483
Fragments arranged by size:
3483 2041 127
MspA1I CmG'CkG
Cuts at: 148 267 293 312 430 556 684 1861 2146
Size: 119 26 19 118 126 128 1177 285
Cuts at: 2146 2762 3076 3436 4449 4694 148
Size: 616 314 360 1013 245 1105
Fragments arranged by size:
1177 1105 1013 616 360 314 285 245
128 126 119 118 26 19
NaeI GCC'GGC
Cuts at: 323 645 3832 323
Size: 322 3187 2142
Fragments arranged by size:
3187 2142 322
NarI GG'CG_CC
Cuts at: 3082 3330 3082
Size: 248 5403
NcoI C'CATG_G
Cuts at: 107 1600 107
Size: 1493 4158
NgoMIV G'CCGG_C
Cuts at: 321 643 3830 321
Size: 322 3187 2142
Fragments arranged by size:
3187 2142 322
NspI r_CATG'y
Cuts at: 1566 2704 3204 4111 1566
Size: 1138 500 907 3106
Fragments arranged by size:
3106 1138 907 500
PciI A'CATG_T
Cuts at: 1562 4107 1562
Size: 2545 3106
PflMI CCAn_nnn'nTGG
Cuts at: 1664 1664
Size: 5651
PfoI T'CCnGG_A
Cuts at: 1674 1674
Size: 5651
PmeI GTTT'AAAC
Cuts at: 2650 2650
Size: 5651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1452 1484 4790 4822 1452
Size: 32 3306 32 2281
Fragments arranged by size:
3306 2281 32 32
PpuMI rG'GwC_Cy
Cuts at: 210 210
Size: 5651
PspOMI G'GGCC_C
Cuts at: 632 632
Size: 5651
PstI C_TGCA'G
Cuts at: 317 1296 2698 3383 317
Size: 979 1402 685 2585
Fragments arranged by size:
2585 1402 979 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5651
PvuII CAG'CTG
Cuts at: 312 1861 2146 3076 3436 312
Size: 1549 285 930 360 2527
Fragments arranged by size:
2527 1549 930 360 285
RsrII CG'GwC_CG
Cuts at: 3846 3846
Size: 5651
SacI G_AGCT'C
Cuts at: 1818 2665 1818
Size: 847 4804
SacII CC_GC'GG
Cuts at: 149 268 149
Size: 119 5532
SalI G'TCGA_C
Cuts at: 2688 2688
Size: 5651
SanDI GG'GwC_CC
Cuts at: 210 210
Size: 5651
SapI GCTCTTCn'nnn_
Cuts at: 3674 3884 3674
Size: 210 5441
SbfI CC_TGCA'GG
Cuts at: 2698 2698
Size: 5651
ScaI AGT'ACT
Cuts at: 5168 5168
Size: 5651
SfcI C'TryA_G
Cuts at: 33 313 1200 1292 1856 2432 2694 3379 4372
Size: 280 887 92 564 576 262 685 993
Cuts at: 4372 4563 33
Size: 191 1121
Fragments arranged by size:
1121 993 887 685 576 564 280 262
191 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 640 649 640
Size: 9 5642
SfoI GGC'GCC
Cuts at: 3083 3331 3083
Size: 248 5403
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
SmaI CCC'GGG
Cuts at: 208 658 2673 208
Size: 450 2015 3186
Fragments arranged by size:
3186 2015 450
SmlI C'TyrA_G
Cuts at: 2806 3721 4213 4475 2806
Size: 915 492 262 3982
Fragments arranged by size:
3982 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5651
SphI G_CATG'C
Cuts at: 2704 3204 2704
Size: 500 5151
SspI AAT'ATT
Cuts at: 2418 2418
Size: 5651
StyI C'CwwG_G
Cuts at: 107 1600 2110 2309 2785 3139 107
Size: 1493 510 199 476 354 2619
Fragments arranged by size:
2619 1493 510 476 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3234 3367 3826 4035 3234
Size: 133 459 209 4850
Fragments arranged by size:
4850 459 209 133
TatI w'GTAC_w
Cuts at: 1591 5166 1591
Size: 3575 2076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 426 458 2542 2574 5168 5200 426
Size: 32 2084 32 2594 32 877
Fragments arranged by size:
2594 2084 877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1431 3448 1431
Size: 2017 3634
XbaI T'CTAG_A
Cuts at: 2682 2682
Size: 5651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3146 3146
Size: 5651
XmaI C'CCGG_G
Cuts at: 206 656 2671 206
Size: 450 2015 3186
Fragments arranged by size:
3186 2015 450
XmnI GAAnn'nnTTC
Cuts at: 1464 1964 2060 1464
Size: 500 96 5055
Fragments arranged by size:
5055 500 96
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BmgBI BmtI BpmI BsiWI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SexAI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI