Restriction map for
pF1KE2450
(Circular) (Six-base) MAPSORT of: pF1KE2450.seq Check: 9311 from: 1 to: 4076
pF1KE2450 4076 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 432 1092 432
Size: 660 3416
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaLI G'TGCA_C
Cuts at: 748 2846 748
Size: 2098 1978
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 607 844 1096 607
Size: 237 252 3587
Fragments arranged by size:
3587 252 237
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 432 687 1092 1506 1754 1789 432
Size: 255 405 414 248 35 2719
Fragments arranged by size:
2719 414 405 255 248 35
BanII G_rGCy'C
Cuts at: 212 1090 2120 212
Size: 878 1030 2168
Fragments arranged by size:
2168 1030 878
BbeI G_GCGC'C
Cuts at: 691 1510 1758 691
Size: 819 248 3009
Fragments arranged by size:
3009 819 248
BbsI GAAGACnn'nnnn_
Cuts at: 378 987 378
Size: 609 3467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4042
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 467 1596 467
Size: 1129 2947
BglI GCCn_nnn'nGGC
Cuts at: 743 743
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 752 1701 1794 2850 752
Size: 949 93 1056 1978
Fragments arranged by size:
1978 1056 949 93
BmrI ACTGGGnnnn_n'
Cuts at: 441 1288 1369 1704 3848 441
Size: 847 81 335 2144 669
Fragments arranged by size:
2144 847 669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 895
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 688 1507 1755 2457 3953 688
Size: 819 248 702 1496 811
Fragments arranged by size:
1496 819 811 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1039 1069 1039
Size: 30 4046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 892 192
Size: 700 3376
BseYI C'CCAG_C
Cuts at: 1501 2259 2836 3332 1501
Size: 758 577 496 2245
Fragments arranged by size:
2245 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 993 811
Size: 182 3894
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 752 821 1090 1868 2058 2850 293
Size: 459 69 269 778 190 792 1519
Fragments arranged by size:
1519 792 778 459 269 190 69
Bsp1286I G_dGCh'C
Cuts at: 212 293 752 821 1090 1701 1794 1868 2058
Size: 81 459 69 269 611 93 74 190
Cuts at: 2058 2120 2850 212
Size: 62 730 1438
Fragments arranged by size:
1438 730 611 459 269 190 93 81
74 69 62
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 722 1126 1340 1642 2023 281
Size: 441 404 214 302 381 2334
Fragments arranged by size:
2334 441 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 1988 890
Size: 1098 2978
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 4076
BssSI C'ACGA_G
Cuts at: 397 2347 2705 397
Size: 1950 358 1768
Fragments arranged by size:
1950 1768 358
BstAPI GCAn_nnn'nTGC
Cuts at: 797 825 1453 797
Size: 28 628 3420
Fragments arranged by size:
3420 628 28
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstXI CCAn_nnnn'nTGG
Cuts at: 873 1044 873
Size: 171 3905
BstYI r'GATC_y
Cuts at: 1 869 1101 1568 1591 1926 2172 3184 3270
Size: 868 232 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 868 467 449 346 335 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 683 732 683
Size: 49 4027
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 1319 1319
Size: 4076
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 662 786 863 1661 1835 2226 2253 3598 662
Size: 124 77 798 174 391 27 1345 1140
Fragments arranged by size:
1345 1140 798 391 174 124 77 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 595 2099 2309 595
Size: 1504 210 2362
Fragments arranged by size:
2362 1504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 288 2284 2594 2740 3886 288
Size: 1996 310 146 1146 478
Fragments arranged by size:
1996 1146 478 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1901 2333 3080 3348 895
Size: 1006 432 747 268 1623
Fragments arranged by size:
1623 1006 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 877 877
Size: 4076
EcoO109I rG'GnC_Cy
Cuts at: 153 852 1215 153
Size: 699 363 3014
Fragments arranged by size:
3014 699 363
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
EcoRV GAT'ATC
Cuts at: 123 123
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 654 683 691 1510 1758 2780 3759 654
Size: 29 8 819 248 1022 979 971
Fragments arranged by size:
1022 979 971 819 248 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1017 1039 1049 1071 1918 1948 1950 1980 1017
Size: 22 10 22 847 30 2 30 3113
Fragments arranged by size:
3113 847 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 687 1506 1754 687
Size: 819 248 3009
Fragments arranged by size:
3009 819 248
KpnI G_GTAC'C
Cuts at: 436 1096 436
Size: 660 3416
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 664 865 1837 664
Size: 201 972 2903
Fragments arranged by size:
2903 972 201
MslI CAynn'nnrTG
Cuts at: 1024 2065 2192 1024
Size: 1041 127 2908
Fragments arranged by size:
2908 1041 127
MspA1I CmG'CkG
Cuts at: 648 734 908 1187 1501 1861 2874 3119 648
Size: 86 174 279 314 360 1013 245 1605
Fragments arranged by size:
1605 1013 360 314 279 245 174 86
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 688 1507 1755 688
Size: 819 248 3009
Fragments arranged by size:
3009 819 248
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 1129 1629 2536 1129
Size: 500 907 2669
Fragments arranged by size:
2669 907 500
PasI CC'CwG_GG
Cuts at: 354 354
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PfoI T'CCnGG_A
Cuts at: 872 872
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 931 963 3215 3247 931
Size: 32 2252 32 1760
Fragments arranged by size:
2252 1760 32 32
PpuMI rG'GwC_Cy
Cuts at: 153 852 153
Size: 699 3377
PshAI GACnn'nnGTC
Cuts at: 559 559
Size: 4076
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 703 735 703
Size: 32 4044
PstI C_TGCA'G
Cuts at: 333 512 658 937 1123 1808 333
Size: 179 146 279 186 685 2601
Fragments arranged by size:
2601 685 279 186 179 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SacII CC_GC'GG
Cuts at: 735 735
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SanDI GG'GwC_CC
Cuts at: 852 852
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 451 3593 451
Size: 3142 934
SfcI C'TryA_G
Cuts at: 33 329 508 654 933 1119 1804 2797 2988
Size: 296 179 146 279 186 685 993 191
Cuts at: 2988 33
Size: 1121
Fragments arranged by size:
1121 993 685 296 279 191 186 179
146
SfoI GGC'GCC
Cuts at: 689 1508 1756 689
Size: 819 248 3009
Fragments arranged by size:
3009 819 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 609 1098 609
Size: 489 3587
SmlI C'TyrA_G
Cuts at: 1231 2146 2638 2900 1231
Size: 915 492 262 2407
Fragments arranged by size:
2407 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StyI C'CwwG_G
Cuts at: 1210 1564 1210
Size: 354 3722
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 981 1659 1792 2251 2460 981
Size: 678 133 459 209 2597
Fragments arranged by size:
2597 678 459 209 133
TatI w'GTAC_w
Cuts at: 449 719 3591 449
Size: 270 2872 934
Fragments arranged by size:
2872 934 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 958 990 3593 3625 958
Size: 32 2603 32 1409
Fragments arranged by size:
2603 1409 32 32
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1571 471
Size: 1100 2976
XmaI C'CCGG_G
Cuts at: 607 1096 607
Size: 489 3587
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PspOMI PspXI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI