Restriction map for
pF1KE2446
(Circular) (Six-base) MAPSORT of: pF1KE2446.seq Check: 3187 from: 1 to: 5258
pF1KE2446 5258 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 2296 2296
Size: 5258
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 5098 5098
Size: 5258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 878 1021 1303 1834 2049 3083 3515 4262
Size: 78 143 282 531 215 1034 432 747
Cuts at: 4262 4530 800
Size: 268 1528
Fragments arranged by size:
1528 1034 747 531 432 282 268 215
143 78
AflIII A'CryG_T
Cuts at: 1757 1759 2610 3714 4691 1757
Size: 2 851 1104 977 2324
Fragments arranged by size:
2324 1104 977 851 2
AgeI A'CCGG_T
Cuts at: 3646 3646
Size: 5258
AlwNI CAG_nnn'CTG
Cuts at: 885 2563 4130 885
Size: 1678 1567 2013
Fragments arranged by size:
2013 1678 1567
ApaI G_GGCC'C
Cuts at: 1290 1290
Size: 5258
ApaLI G'TGCA_C
Cuts at: 4028 4028
Size: 5258
ApoI r'AATT_y
Cuts at: 271 616 721 1383 1408 2262 4611 4867 271
Size: 345 105 662 25 854 2349 256 662
Fragments arranged by size:
2349 854 662 662 345 256 105 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 5258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 160 2278 160
Size: 2118 3140
BamHI G'GATC_C
Cuts at: 645 2283 645
Size: 1638 3620
BanI G'GyrC_C
Cuts at: 142 2222 2274 2688 2936 2971 142
Size: 2080 52 414 248 35 2429
Fragments arranged by size:
2429 2080 414 248 52 35
BanII G_rGCy'C
Cuts at: 1290 1784 2272 3302 1290
Size: 494 488 1030 3246
Fragments arranged by size:
3246 1030 494 488
BbeI G_GCGC'C
Cuts at: 2692 2940 2692
Size: 248 5010
BbsI GAAGACnn'nnnn_
Cuts at: 2014 2014
Size: 5258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1495 1529 1495
Size: 34 5224
BciVI GTATCCnnnnn_n'
Cuts at: 1926 3156 3917 5258 1926
Size: 1230 761 1341 1926
Fragments arranged by size:
1926 1341 1230 761
BclI T'GATC_A
Cuts at: 2778 2778
Size: 5258
BglI GCCn_nnn'nGGC
Cuts at: 1795 1795
Size: 5258
BglII A'GATC_T
Cuts at: 932 1851 2773 4810 932
Size: 919 922 2037 1380
Fragments arranged by size:
2037 1380 922 919
BlpI GC'TnA_GC
Cuts at: 2370 2370
Size: 5258
Bme1580I G_kGCm'C
Cuts at: 1290 2883 2976 4032 1290
Size: 1593 93 1056 2516
Fragments arranged by size:
2516 1593 1056 93
BmgBI CAC'GTC
Cuts at: 1485 1485
Size: 5258
BmrI ACTGGGnnnn_n'
Cuts at: 1139 2219 2470 2551 2886 5030 1139
Size: 1080 251 81 335 2144 1367
Fragments arranged by size:
2144 1367 1080 335 251 81
BmtI G_CTAG'C
Cuts at: 791 791
Size: 5258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3625 3625
Size: 5258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 1360 1379 1618 1669 1897 1264
Size: 96 19 239 51 228 4625
Fragments arranged by size:
4625 239 228 96 51 19
Bpu10I CC'TnA_GC
Cuts at: 1952 1952
Size: 5258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2434 3313 3805 4103 1540
Size: 894 879 492 298 2695
Fragments arranged by size:
2695 894 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 695 1209 1862 4962 695
Size: 514 653 3100 991
Fragments arranged by size:
3100 991 653 514
BsaAI yAC'GTr
Cuts at: 1760 2611 1760
Size: 851 4407
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 2689 2937 3639 5135 2689
Size: 248 702 1496 2812
Fragments arranged by size:
2812 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1196 2447 2968 3646 3920 4067 4478 1196
Size: 1251 521 678 274 147 411 1976
Fragments arranged by size:
1976 1251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1246 1276 2062 2092 1246
Size: 30 786 30 4412
Fragments arranged by size:
4412 786 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 1068 1615 1666 1669 586
Size: 482 547 51 3 4175
Fragments arranged by size:
4175 547 482 51 3
BseYI C'CCAG_C
Cuts at: 138 1208 1643 2683 3441 4018 4514 138
Size: 1070 435 1040 758 577 496 882
Fragments arranged by size:
1070 1040 882 758 577 496 435
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 105
Size: 5258
BsiEI CG_ry'CG
Cuts at: 104 2800 2846 4054 104
Size: 2696 46 1208 1308
Fragments arranged by size:
2696 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 285 1784 2272 3050 3240 4032 285
Size: 1499 488 778 190 792 1511
Fragments arranged by size:
1511 1499 792 778 488 190
Bsp1286I G_dGCh'C
Cuts at: 285 1290 1784 2272 2883 2976 3050 3240 3302
Size: 1005 494 488 611 93 74 190 62
Cuts at: 3302 4032 285
Size: 730 1511
Fragments arranged by size:
1511 1005 730 611 494 488 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4478 4478
Size: 5258
BspHI T'CATG_A
Cuts at: 803 4434 803
Size: 3631 1627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 713 893 954 2308 2522 2824 3205 713
Size: 180 61 1354 214 302 381 2766
Fragments arranged by size:
2766 1354 381 302 214 180 61
BsrBI CCG'CTC
Cuts at: 50 3550 3604 5088 5253 50
Size: 3500 54 1484 165 55
Fragments arranged by size:
3500 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1533 1550 3170 1533
Size: 17 1620 3621
Fragments arranged by size:
3621 1620 17
BsrFI r'CCGG_y
Cuts at: 3256 3437 3646 3256
Size: 181 209 4868
Fragments arranged by size:
4868 209 181
BssSI C'ACGA_G
Cuts at: 3529 3887 3529
Size: 358 4900
BstAPI GCAn_nnn'nTGC
Cuts at: 2635 2635
Size: 5258
BstBI TT'CG_AA
Cuts at: 3619 3619
Size: 5258
BstEII G'GTnAC_C
Cuts at: 1992 1992
Size: 5258
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5258
BstYI r'GATC_y
Cuts at: 1 645 932 1851 2283 2750 2773 3108 3354
Size: 644 287 919 432 467 23 335 246
Cuts at: 3354 4366 4452 4464 4810 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 919 644 467 449 432 346 335
287 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1108 1108
Size: 5258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3208 3389 3552 3208
Size: 181 163 4914
Fragments arranged by size:
4914 181 163
BtsI GCAGTG_nn'
Cuts at: 2501 2501
Size: 5258
DraI TTT'AAA
Cuts at: 692 2257 692
Size: 1565 3693
DrdI GACnn_nn'nnGTC
Cuts at: 1201 2964 3822 1201
Size: 1763 858 2637
Fragments arranged by size:
2637 1763 858
EaeI y'GGCC_r
Cuts at: 957 1796 2843 3017 3408 3435 4780 957
Size: 839 1047 174 391 27 1345 1435
Fragments arranged by size:
1435 1345 1047 839 391 174 27
EagI C'GGCC_G
Cuts at: 2843 2843
Size: 5258
EarI CTCTTCn'nnn_
Cuts at: 746 854 1017 1021 1322 2120 3281 3491 746
Size: 108 163 4 301 798 1161 210 2513
Fragments arranged by size:
2513 1161 798 301 210 163 108 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3466 3776 3922 5068 3466
Size: 310 146 1146 3656
Fragments arranged by size:
3656 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1782 2270 1782
Size: 488 4770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 878 1021 1264 1303 1360 1379 1618 1669
Size: 78 143 243 39 57 19 239 51
Cuts at: 1669 1834 1897 2049 3083 3515 4262 4530 800
Size: 165 63 152 1034 432 747 268 1528
Fragments arranged by size:
1528 1034 747 432 268 243 239 165
152 143 78 63 57 51 39 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1083 1083
Size: 5258
EcoO109I rG'GnC_Cy
Cuts at: 882 1286 2397 882
Size: 404 1111 3743
Fragments arranged by size:
3743 1111 404
EcoRI G'AATT_C
Cuts at: 721 2262 721
Size: 1541 3717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 931 3277 931
Size: 2346 2912
FspI TGC'GCA
Cuts at: 709 3039 709
Size: 2330 2928
HaeII r_GCGC'y
Cuts at: 160 2692 2940 3962 4941 160
Size: 2532 248 1022 979 477
Fragments arranged by size:
2532 1022 979 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 655 687 785 817 2062 2094 3100
Size: 32 331 32 98 32 1245 32 1006
Cuts at: 3100 3130 3132 3162 292
Size: 30 2 30 2388
Fragments arranged by size:
2388 1245 1006 331 98 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 825 2297 825
Size: 1472 3786
HindIII A'AGCT_T
Cuts at: 694 694
Size: 5258
KasI G'GCGC_C
Cuts at: 2688 2936 2688
Size: 248 5010
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MluI A'CGCG_T
Cuts at: 4691 4691
Size: 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1289 1421 3929 4113 1001
Size: 288 132 2508 184 2146
Fragments arranged by size:
2508 2146 288 184 132
MscI TGG'CCA
Cuts at: 959 3019 959
Size: 2060 3198
MslI CAynn'nnrTG
Cuts at: 3247 3374 3247
Size: 127 5131
MspA1I CmG'CkG
Cuts at: 1329 1509 1647 2055 2369 2683 3043 4056 4301
Size: 180 138 408 314 314 360 1013 245
Cuts at: 4301 1329
Size: 2286
Fragments arranged by size:
2286 1013 408 360 314 314 245 180
138
NaeI GCC'GGC
Cuts at: 3439 3439
Size: 5258
NarI GG'CG_CC
Cuts at: 2689 2937 2689
Size: 248 5010
NgoMIV G'CCGG_C
Cuts at: 3437 3437
Size: 5258
NheI G'CTAG_C
Cuts at: 787 787
Size: 5258
NspI r_CATG'y
Cuts at: 1476 2311 2811 3718 1476
Size: 835 500 907 3016
Fragments arranged by size:
3016 907 835 500
PciI A'CATG_T
Cuts at: 3714 3714
Size: 5258
PflMI CCAn_nnn'nTGG
Cuts at: 774 774
Size: 5258
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PmlI CAC'GTG
Cuts at: 1760 1760
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 404 436 4397 4429 404
Size: 32 3961 32 1233
Fragments arranged by size:
3961 1233 32 32
PpuMI rG'GwC_Cy
Cuts at: 882 882
Size: 5258
PspOMI G'GGCC_C
Cuts at: 1286 1286
Size: 5258
PstI C_TGCA'G
Cuts at: 1745 2305 2990 1745
Size: 560 685 4013
Fragments arranged by size:
4013 685 560
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 1329 1509 1647 2055 2683 3043 1329
Size: 180 138 408 628 360 3544
Fragments arranged by size:
3544 628 408 360 180 138
RsrII CG'GwC_CG
Cuts at: 3453 3453
Size: 5258
SacI G_AGCT'C
Cuts at: 1784 2272 1784
Size: 488 4770
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 1017 2120 3281 3491 1017
Size: 1103 1161 210 2784
Fragments arranged by size:
2784 1161 1103 210
SbfI CC_TGCA'GG
Cuts at: 2305 2305
Size: 5258
ScaI AGT'ACT
Cuts at: 1075 4775 1075
Size: 3700 1558
SfcI C'TryA_G
Cuts at: 33 896 1741 2301 2986 3979 4170 33
Size: 863 845 560 685 993 191 1121
Fragments arranged by size:
1121 993 863 845 685 560 191
SfoI GGC'GCC
Cuts at: 2690 2938 2690
Size: 248 5010
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 2280 2280
Size: 5258
SmlI C'TyrA_G
Cuts at: 1519 2413 3328 3820 4082 1519
Size: 894 915 492 262 2695
Fragments arranged by size:
2695 915 894 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2311 2811 2311
Size: 500 4758
StyI C'CwwG_G
Cuts at: 1676 2392 2746 1676
Size: 716 354 4188
Fragments arranged by size:
4188 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 2240 2841 2974 3433 3642 130
Size: 2110 601 133 459 209 1746
Fragments arranged by size:
2110 1746 601 459 209 133
TatI w'GTAC_w
Cuts at: 853 1073 4773 853
Size: 220 3700 1338
Fragments arranged by size:
3700 1338 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4775 4807 4775
Size: 32 5226
Tth111I GACn'n_nGTC
Cuts at: 1131 3055 1131
Size: 1924 3334
XbaI T'CTAG_A
Cuts at: 2289 2289
Size: 5258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1872 2753 1872
Size: 881 4377
XmaI C'CCGG_G
Cuts at: 2278 2278
Size: 5258
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI