Restriction map for
pF1KE2443
(Circular) (Six-base) MAPSORT of: pF1KE2443.seq Check: 6206 from: 1 to: 4076
pF1KE2443 4076 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 648 1114 648
Size: 466 3610
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 4076
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 727
Size: 32 4044
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaLI G'TGCA_C
Cuts at: 400 2846 400
Size: 2446 1630
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 1096 1096
Size: 4076
BamHI G'GATC_C
Cuts at: 179 209 1101 179
Size: 30 892 3154
Fragments arranged by size:
3154 892 30
BanI G'GyrC_C
Cuts at: 1092 1506 1754 1789 1092
Size: 414 248 35 3379
Fragments arranged by size:
3379 414 248 35
BanII G_rGCy'C
Cuts at: 969 1090 2120 969
Size: 121 1030 2925
Fragments arranged by size:
2925 1030 121
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 339 435 862 339
Size: 96 427 3553
Fragments arranged by size:
3553 427 96
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 404 1701 1794 2850 404
Size: 1297 93 1056 1630
Fragments arranged by size:
1630 1297 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 351 368 506 1288 1369 1704 3848 351
Size: 17 138 782 81 335 2144 579
Fragments arranged by size:
2144 782 579 335 138 81 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 509
Size: 4076
Bpu10I CC'TnA_GC
Cuts at: 968 968
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2623 2921 1252
Size: 879 492 298 2407
Fragments arranged by size:
2407 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 455 3780 455
Size: 3325 751
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 206 1265 1786 2464 2738 2885 3296 206
Size: 1059 521 678 274 147 411 986
Fragments arranged by size:
1059 986 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 226
Size: 30 4046
BseYI C'CCAG_C
Cuts at: 1501 2259 2836 3332 1501
Size: 758 577 496 2245
Fragments arranged by size:
2245 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 4076
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 404 1090 1868 2058 2850 404
Size: 686 778 190 792 1630
Fragments arranged by size:
1630 792 778 686 190
BsmBI CGTCTCn'nnnn_
Cuts at: 229 229
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 404 969 1090 1701 1794 1868 2058 2120 2850
Size: 565 121 611 93 74 190 62 730
Cuts at: 2850 404
Size: 1630
Fragments arranged by size:
1630 730 611 565 190 121 93 74
62
BspEI T'CCGG_A
Cuts at: 206 3296 206
Size: 3090 986
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1019 1126 1340 1642 2023 1019
Size: 107 214 302 381 3072
Fragments arranged by size:
3072 381 302 214 107
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1988 1988
Size: 4076
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstYI r'GATC_y
Cuts at: 1 179 209 1101 1568 1591 1926 2172 3184
Size: 178 30 892 467 23 335 246 1012
Cuts at: 3184 3270 3282 3628 1
Size: 86 12 346 449
Fragments arranged by size:
1012 892 467 449 346 335 246 178
86 30 23 12
BtgI C'CryG_G
Cuts at: 107 146 107
Size: 39 4037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 516 653 1319 516
Size: 137 666 3273
Fragments arranged by size:
3273 666 137
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 221 753 844 1661 1835 2226 2253 3598 221
Size: 532 91 817 174 391 27 1345 699
Fragments arranged by size:
1345 817 699 532 391 174 91 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 399 2284 2594 2740 3886 399
Size: 1885 310 146 1146 589
Fragments arranged by size:
1885 1146 589 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1901 2333 3080 3348 509
Size: 1392 432 747 268 1237
Fragments arranged by size:
1392 1237 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 484 1008 1215 484
Size: 524 207 3345
Fragments arranged by size:
3345 524 207
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 879 2095 879
Size: 1216 2860
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 1510 1758 2780 3759 1510
Size: 248 1022 979 1827
Fragments arranged by size:
1827 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 188 1115 188
Size: 927 3149
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 223 755 1837 223
Size: 532 1082 2462
Fragments arranged by size:
2462 1082 532
MslI CAynn'nnrTG
Cuts at: 265 361 2065 2192 265
Size: 96 1704 127 2149
Fragments arranged by size:
2149 1704 127 96
MspA1I CmG'CkG
Cuts at: 148 1187 1501 1861 2874 3119 148
Size: 1039 314 360 1013 245 1105
Fragments arranged by size:
1105 1039 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 107 107
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 1129 1629 2536 1129
Size: 500 907 2669
Fragments arranged by size:
2669 907 500
PasI CC'CwG_GG
Cuts at: 784 784
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PfoI T'CCnGG_A
Cuts at: 486 486
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PpuMI rG'GwC_Cy
Cuts at: 484 1008 484
Size: 524 3552
PstI C_TGCA'G
Cuts at: 284 572 1123 1808 284
Size: 288 551 685 2552
Fragments arranged by size:
2552 685 551 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SacII CC_GC'GG
Cuts at: 149 149
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 280 568 1119 1804 2797 2988 33
Size: 247 288 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 288 247 191
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 1231 2146 2638 2900 1231
Size: 915 492 262 2407
Fragments arranged by size:
2407 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
SspI AAT'ATT
Cuts at: 760 760
Size: 4076
StuI AGG'CCT
Cuts at: 301 301
Size: 4076
StyI C'CwwG_G
Cuts at: 107 1015 1210 1564 107
Size: 908 195 354 2619
Fragments arranged by size:
2619 908 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 653 1659 1792 2251 2460 653
Size: 1006 133 459 209 2269
Fragments arranged by size:
2269 1006 459 209 133
TatI w'GTAC_w
Cuts at: 903 3591 903
Size: 2688 1388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1047 1873 1047
Size: 826 3250
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI