Restriction map for
pF1KE2439
(Circular) (Six-base) MAPSORT of: pF1KE2439.seq Check: 5366 from: 1 to: 3542
pF1KE2439 3542 bp
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 295 295
Size: 3542
AccI GT'mk_AC
Cuts at: 580 580
Size: 3542
Acc65I G'GTAC_C
Cuts at: 558 558
Size: 3542
AclI AA'CG_TT
Cuts at: 3382 3382
Size: 3542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1367 1799 2546 2814 448
Size: 919 432 747 268 1176
Fragments arranged by size:
1176 919 747 432 268
AflIII A'CryG_T
Cuts at: 894 1998 2975 894
Size: 1104 977 1461
Fragments arranged by size:
1461 1104 977
AgeI A'CCGG_T
Cuts at: 1930 1930
Size: 3542
AlwNI CAG_nnn'CTG
Cuts at: 847 2414 847
Size: 1567 1975
ApaI G_GGCC'C
Cuts at: 239 297 239
Size: 58 3484
ApaLI G'TGCA_C
Cuts at: 2312 2312
Size: 3542
ApoI r'AATT_y
Cuts at: 546 2895 3151 546
Size: 2349 256 937
Fragments arranged by size:
2349 937 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3542
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3542
AvaI C'yCGr_G
Cuts at: 204 562 204
Size: 358 3184
BamHI G'GATC_C
Cuts at: 567 567
Size: 3542
BanI G'GyrC_C
Cuts at: 283 321 403 421 558 972 1220 1255 283
Size: 38 82 18 137 414 248 35 2570
Fragments arranged by size:
2570 414 248 137 82 38 35 18
BanII G_rGCy'C
Cuts at: 239 297 556 1586 239
Size: 58 259 1030 2195
Fragments arranged by size:
2195 1030 259 58
BbeI G_GCGC'C
Cuts at: 976 1224 976
Size: 248 3294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3508
BciVI GTATCCnnnnn_n'
Cuts at: 1440 2201 3542 1440
Size: 761 1341 1440
Fragments arranged by size:
1440 1341 761
BclI T'GATC_A
Cuts at: 1062 1062
Size: 3542
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 3542
BglII A'GATC_T
Cuts at: 1057 3094 1057
Size: 2037 1505
BlpI GC'TnA_GC
Cuts at: 654 654
Size: 3542
Bme1580I G_kGCm'C
Cuts at: 239 286 297 326 400 1167 1260 2316 239
Size: 47 11 29 74 767 93 1056 1465
Fragments arranged by size:
1465 1056 767 93 74 47 29 11
BmrI ACTGGGnnnn_n'
Cuts at: 387 754 835 1170 3314 387
Size: 367 81 335 2144 615
Fragments arranged by size:
2144 615 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 1909 142
Size: 1767 1775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1597 2089 2387 718
Size: 879 492 298 1873
Fragments arranged by size:
1873 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 454 3246 454
Size: 2792 750
BsaAI yAC'GTr
Cuts at: 895 895
Size: 3542
BsaBI GATnn'nnATC
Cuts at: 6 1056 3093 6
Size: 1050 2037 455
Fragments arranged by size:
2037 1050 455
BsaHI Gr'CG_yC
Cuts at: 973 1221 1923 3419 973
Size: 248 702 1496 1096
Fragments arranged by size:
1496 1096 702 248
BsaWI w'CCGG_w
Cuts at: 311 731 1252 1930 2204 2351 2762 311
Size: 420 521 678 274 147 411 1091
Fragments arranged by size:
1091 678 521 420 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 121 130 118
Size: 3 9 3530
Fragments arranged by size:
3530 9 3
BseYI C'CCAG_C
Cuts at: 247 302 967 1725 2302 2798 247
Size: 55 665 758 577 496 991
Fragments arranged by size:
991 758 665 577 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 336 468 225
Size: 111 132 3299
Fragments arranged by size:
3299 132 111
BsiEI CG_ry'CG
Cuts at: 104 170 1084 1130 2338 104
Size: 66 914 46 1208 1308
Fragments arranged by size:
1308 1208 914 66 46
BsiHKAI G_wGCw'C
Cuts at: 556 1334 1524 2316 556
Size: 778 190 792 1782
Fragments arranged by size:
1782 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 239 286 297 326 400 556 1167 1260 1334
Size: 47 11 29 74 156 611 93 74
Cuts at: 1334 1524 1586 2316 239
Size: 190 62 730 1465
Fragments arranged by size:
1465 730 611 190 156 93 74 74
62 47 29 11
BspEI T'CCGG_A
Cuts at: 2762 2762
Size: 3542
BspHI T'CATG_A
Cuts at: 2718 2718
Size: 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 592 806 1108 1489 295
Size: 297 214 302 381 2348
Fragments arranged by size:
2348 381 302 297 214
BsrBI CCG'CTC
Cuts at: 50 1834 1888 3372 3537 50
Size: 1784 54 1484 165 55
Fragments arranged by size:
1784 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1454 1454
Size: 3542
BsrFI r'CCGG_y
Cuts at: 1540 1721 1930 1540
Size: 181 209 3152
Fragments arranged by size:
3152 209 181
BssHII G'CGCG_C
Cuts at: 436 436
Size: 3542
BssSI C'ACGA_G
Cuts at: 1813 2171 1813
Size: 358 3184
BstAPI GCAn_nnn'nTGC
Cuts at: 260 314 919 260
Size: 54 605 2883
Fragments arranged by size:
2883 605 54
BstBI TT'CG_AA
Cuts at: 1903 1903
Size: 3542
BstYI r'GATC_y
Cuts at: 1 567 1034 1057 1392 1638 2650 2736 2748
Size: 566 467 23 335 246 1012 86 12
Cuts at: 2748 3094 1
Size: 346 449
Fragments arranged by size:
1012 566 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 164 164
Size: 3542
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1492 1673 1836 1492
Size: 181 163 3198
Fragments arranged by size:
3198 181 163
BtsI GCAGTG_nn'
Cuts at: 785 785
Size: 3542
DraI TTT'AAA
Cuts at: 541 541
Size: 3542
DrdI GACnn_nn'nnGTC
Cuts at: 1248 2106 1248
Size: 858 2684
EaeI y'GGCC_r
Cuts at: 1127 1301 1692 1719 3064 1127
Size: 174 391 27 1345 1605
Fragments arranged by size:
1605 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1127 1127
Size: 3542
EarI CTCTTCn'nnn_
Cuts at: 1565 1775 1565
Size: 210 3332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 403 1750 2060 2206 3352 403
Size: 1347 310 146 1146 593
Fragments arranged by size:
1347 1146 593 310 146
EcoICRI GAG'CTC
Cuts at: 554 554
Size: 3542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1367 1799 2546 2814 448
Size: 919 432 747 268 1176
Fragments arranged by size:
1176 919 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 430 430
Size: 3542
EcoO109I rG'GnC_Cy
Cuts at: 235 236 293 681 235
Size: 1 57 388 3096
Fragments arranged by size:
3096 388 57 1
EcoRI G'AATT_C
Cuts at: 546 546
Size: 3542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1561 1561
Size: 3542
FspI TGC'GCA
Cuts at: 1323 1323
Size: 3542
HaeII r_GCGC'y
Cuts at: 157 976 1224 2246 3225 157
Size: 819 248 1022 979 474
Fragments arranged by size:
1022 979 819 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1384 1414 1416 1446 1384
Size: 30 2 30 3480
Fragments arranged by size:
3480 30 30 2
HincII GTy'rAC
Cuts at: 581 581
Size: 3542
KasI G'GCGC_C
Cuts at: 972 1220 972
Size: 248 3294
KpnI G_GTAC'C
Cuts at: 562 562
Size: 3542
MluI A'CGCG_T
Cuts at: 2975 2975
Size: 3542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2397 2213
Size: 184 3358
MscI TGG'CCA
Cuts at: 1303 1303
Size: 3542
MslI CAynn'nnrTG
Cuts at: 1531 1658 1531
Size: 127 3415
MspA1I CmG'CkG
Cuts at: 166 653 967 1327 2340 2585 166
Size: 487 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 487 360 314 245
NaeI GCC'GGC
Cuts at: 1723 1723
Size: 3542
NarI GG'CG_CC
Cuts at: 973 1221 973
Size: 248 3294
NgoMIV G'CCGG_C
Cuts at: 1721 1721
Size: 3542
NspI r_CATG'y
Cuts at: 595 1095 2002 595
Size: 500 907 2135
Fragments arranged by size:
2135 907 500
PciI A'CATG_T
Cuts at: 1998 1998
Size: 3542
PmeI GTTT'AAAC
Cuts at: 541 541
Size: 3542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 2681
Size: 32 3510
PspOMI G'GGCC_C
Cuts at: 235 293 235
Size: 58 3484
PstI C_TGCA'G
Cuts at: 589 1274 589
Size: 685 2857
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3542
PvuII CAG'CTG
Cuts at: 967 1327 967
Size: 360 3182
RsrII CG'GwC_CG
Cuts at: 1737 1737
Size: 3542
SacI G_AGCT'C
Cuts at: 556 556
Size: 3542
SacII CC_GC'GG
Cuts at: 167 167
Size: 3542
SalI G'TCGA_C
Cuts at: 579 579
Size: 3542
SapI GCTCTTCn'nnn_
Cuts at: 1565 1775 1565
Size: 210 3332
SbfI CC_TGCA'GG
Cuts at: 589 589
Size: 3542
ScaI AGT'ACT
Cuts at: 3059 3059
Size: 3542
SfcI C'TryA_G
Cuts at: 33 585 1270 2263 2454 33
Size: 552 685 993 191 1121
Fragments arranged by size:
1121 993 685 552 191
SfoI GGC'GCC
Cuts at: 974 1222 974
Size: 248 3294
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3542
SmaI CCC'GGG
Cuts at: 206 564 206
Size: 358 3184
SmlI C'TyrA_G
Cuts at: 697 1612 2104 2366 697
Size: 915 492 262 1873
Fragments arranged by size:
1873 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3542
SphI G_CATG'C
Cuts at: 595 1095 595
Size: 500 3042
StyI C'CwwG_G
Cuts at: 417 676 1030 417
Size: 259 354 2929
Fragments arranged by size:
2929 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1125 1258 1717 1926 1125
Size: 133 459 209 2741
Fragments arranged by size:
2741 459 209 133
TatI w'GTAC_w
Cuts at: 362 3057 362
Size: 2695 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3510
Tth111I GACn'n_nGTC
Cuts at: 1339 1339
Size: 3542
XbaI T'CTAG_A
Cuts at: 573 573
Size: 3542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1037 1037
Size: 3542
XmaI C'CCGG_G
Cuts at: 204 562 204
Size: 358 3184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI