Restriction map for
pF1KE2438
(Circular) (Six-base) MAPSORT of: pF1KE2438.seq Check: 7606 from: 1 to: 8936
pF1KE2438 8936 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 1409 5974 1409
Size: 4565 4371
Acc65I G'GTAC_C
Cuts at: 5952 5952
Size: 8936
AclI AA'CG_TT
Cuts at: 432 8776 432
Size: 8344 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2339 2825 3581 4639 4729 4903 5146 5452
Size: 321 486 756 1058 90 174 243 306
Cuts at: 5452 5560 5722 6761 7193 7940 8208 2018
Size: 108 162 1039 432 747 268 2746
Fragments arranged by size:
2746 1058 1039 756 747 486 432 321
306 268 243 174 162 108 90
AfeI AGC'GCT
Cuts at: 3532 3532
Size: 8936
AflII C'TTAA_G
Cuts at: 946 946
Size: 8936
AflIII A'CryG_T
Cuts at: 1592 4332 6288 7392 8369 1592
Size: 2740 1956 1104 977 2159
Fragments arranged by size:
2740 2159 1956 1104 977
AgeI A'CCGG_T
Cuts at: 7324 7324
Size: 8936
AleI CACnn'nnGTG
Cuts at: 4142 4142
Size: 8936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 422 3451 422
Size: 3029 5907
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1717 1749 3619 3651 5225 5257 1717
Size: 32 1870 32 1574 32 5396
Fragments arranged by size:
5396 1870 1574 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 712 1284 1455 1785 2068 2659 3144 3774 3930
Size: 572 171 330 283 591 485 630 156
Cuts at: 3930 4314 4386 5271 5568 6241 7808 712
Size: 384 72 885 297 673 1567 1840
Fragments arranged by size:
1840 1567 885 673 630 591 572 485
384 330 297 283 171 156 72
ApaLI G'TGCA_C
Cuts at: 7706 7706
Size: 8936
ApoI r'AATT_y
Cuts at: 853 1204 2085 2395 2689 5809 5940 8289 8545
Size: 351 881 310 294 3120 131 2349 256
Cuts at: 8545 853
Size: 1244
Fragments arranged by size:
3120 2349 1244 881 351 310 294 256
131
AseI AT'TA_AT
Cuts at: 20 20
Size: 8936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8936
AvaI C'yCGr_G
Cuts at: 517 3509 3545 5956 517
Size: 2992 36 2411 3497
Fragments arranged by size:
3497 2992 2411 36
BamHI G'GATC_C
Cuts at: 5961 5961
Size: 8936
BanI G'GyrC_C
Cuts at: 2170 3959 5952 6366 6614 6649 2170
Size: 1789 1993 414 248 35 4457
Fragments arranged by size:
4457 1993 1789 414 248 35
BanII G_rGCy'C
Cuts at: 236 1685 1989 2050 2441 5243 5950 6980 236
Size: 1449 304 61 391 2802 707 1030 2192
Fragments arranged by size:
2802 2192 1449 1030 707 391 304 61
BbeI G_GCGC'C
Cuts at: 3963 6370 6618 3963
Size: 2407 248 6281
Fragments arranged by size:
6281 2407 248
BbsI GAAGACnn'nnnn_
Cuts at: 301 330 667 2422 4121 301
Size: 29 337 1755 1699 5116
Fragments arranged by size:
5116 1755 1699 337 29
BbvCI CC'TCA_GC
Cuts at: 2203 2903 4325 5036 5423 5627 5771 5780 2203
Size: 700 1422 711 387 204 144 9 5359
Fragments arranged by size:
5359 1422 711 700 387 204 144 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5208 5242 5208
Size: 34 8902
BciVI GTATCCnnnnn_n'
Cuts at: 2575 4702 5073 6834 7595 8936 2575
Size: 2127 371 1761 761 1341 2575
Fragments arranged by size:
2575 2127 1761 1341 761 371
BclI T'GATC_A
Cuts at: 330 635 1089 3932 5294 6456 330
Size: 305 454 2843 1362 1162 2810
Fragments arranged by size:
2843 2810 1362 1162 454 305
BfrBI ATG'CAT
Cuts at: 1188 1188
Size: 8936
BglI GCCn_nnn'nGGC
Cuts at: 3511 3589 3691 5395 5419 3511
Size: 78 102 1704 24 7028
Fragments arranged by size:
7028 1704 102 78 24
BglII A'GATC_T
Cuts at: 1296 6451 8488 1296
Size: 5155 2037 1744
Fragments arranged by size:
5155 2037 1744
BlpI GC'TnA_GC
Cuts at: 1970 2927 3095 4520 5525 6048 1970
Size: 957 168 1425 1005 523 4858
Fragments arranged by size:
4858 1425 1005 957 523 168
Bme1580I G_kGCm'C
Cuts at: 2405 6561 6654 7710 2405
Size: 4156 93 1056 3631
Fragments arranged by size:
4156 3631 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2382 6148 6229 6564 8708 2382
Size: 3766 81 335 2144 2610
Fragments arranged by size:
3766 2610 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3522 7303 3522
Size: 3781 5155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 817 1261 1555 1624 1918 2974 3106 3499
Size: 155 444 294 69 294 1056 132 393
Cuts at: 3499 3574 3679 3796 4303 4723 5335 5344 5482
Size: 75 105 117 507 420 612 9 138
Cuts at: 5482 5590 5806 5854 662
Size: 108 216 48 3744
Fragments arranged by size:
3744 1056 612 507 444 420 393 294
294 216 155 138 132 117 108 105
75 69 48 9
Bpu10I CC'TnA_GC
Cuts at: 2203 2903 4325 5036 5423 5627 5771 5780 2203
Size: 700 1422 711 387 204 144 9 5359
Fragments arranged by size:
5359 1422 711 700 387 204 144 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2959 3016 3310 4546 4834 4951 5059 6112 6991
Size: 57 294 1236 288 117 108 1053 879
Cuts at: 6991 7483 7781 2959
Size: 492 298 4114
Fragments arranged by size:
4114 1236 1053 879 492 298 294 288
117 108 57
BsaI GGTCTCn'nnnn_
Cuts at: 472 1161 1918 3843 4979 8640 472
Size: 689 757 1925 1136 3661 768
Fragments arranged by size:
3661 1925 1136 768 757 689
BsaAI yAC'GTr
Cuts at: 6289 6289
Size: 8936
BsaBI GATnn'nnATC
Cuts at: 6 6450 8487 6
Size: 6444 2037 455
Fragments arranged by size:
6444 2037 455
BsaHI Gr'CG_yC
Cuts at: 3960 4259 6367 6615 7317 8813 3960
Size: 299 2108 248 702 1496 4083
Fragments arranged by size:
4083 2108 1496 702 299 248
BsaWI w'CCGG_w
Cuts at: 5108 6125 6646 7324 7598 7745 8156 5108
Size: 1017 521 678 274 147 411 5888
Fragments arranged by size:
5888 1017 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 269 299 559 589 1528 1558 1717 1747 2148
Size: 30 260 30 939 30 159 30 401
Cuts at: 2148 2178 3619 3649 5225 5255 269
Size: 30 1441 30 1576 30 3950
Fragments arranged by size:
3950 1576 1441 939 401 260 159 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 320 828 1240 1627 1738 2461 3178 3487 3496
Size: 508 412 387 111 723 717 309 9
Cuts at: 3496 3571 3676 3931 4102 4183 4186 4291 4300
Size: 75 105 255 171 81 3 105 9
Cuts at: 4300 5197 5229 5440 5788 5851 320
Size: 897 32 211 348 63 3405
Fragments arranged by size:
3405 897 723 717 508 412 387 348
309 255 211 171 111 105 105 81
75 63 32 9 9 3
BseYI C'CCAG_C
Cuts at: 2471 4307 6361 7119 7696 8192 2471
Size: 1836 2054 758 577 496 3215
Fragments arranged by size:
3215 2054 1836 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 2520 2672 5257 674
Size: 1846 152 2585 4353
Fragments arranged by size:
4353 2585 1846 152
BsiEI CG_ry'CG
Cuts at: 104 6478 6524 7732 104
Size: 6374 46 1208 1308
Fragments arranged by size:
6374 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1395 1685 4206 4924 5243 5950 6728 6918 7710
Size: 290 2521 718 319 707 778 190 792
Cuts at: 7710 1395
Size: 2621
Fragments arranged by size:
2621 2521 792 778 718 707 319 290
190
BsmI GAATG_Cn'
Cuts at: 3428 4587 3428
Size: 1159 7777
BsmBI CGTCTCn'nnnn_
Cuts at: 4246 4246
Size: 8936
Bsp1286I G_dGCh'C
Cuts at: 236 1395 1685 1989 2050 2405 2441 4206 4924
Size: 1159 290 304 61 355 36 1765 718
Cuts at: 4924 5243 5950 6561 6654 6728 6918 6980 7710
Size: 319 707 611 93 74 190 62 730
Cuts at: 7710 236
Size: 1462
Fragments arranged by size:
1765 1462 1159 730 718 707 611 355
319 304 290 190 93 74 62 61
36
BspEI T'CCGG_A
Cuts at: 5108 8156 5108
Size: 3048 5888
BspHI T'CATG_A
Cuts at: 2588 8112 2588
Size: 5524 3412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3064 3161 3761 4157 5171 5986 6200 6502 6883
Size: 97 600 396 1014 815 214 302 381
Cuts at: 6883 3064
Size: 5117
Fragments arranged by size:
5117 1014 815 600 396 381 302 214
97
BsrBI CCG'CTC
Cuts at: 50 3501 5466 7228 7282 8766 8931 50
Size: 3451 1965 1762 54 1484 165 55
Fragments arranged by size:
3451 1965 1762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1344 1499 1961 3089 5379 5858 6848 1344
Size: 155 462 1128 2290 479 990 3432
Fragments arranged by size:
3432 2290 1128 990 479 462 155
BsrFI r'CCGG_y
Cuts at: 3583 6934 7115 7324 3583
Size: 3351 181 209 5195
Fragments arranged by size:
5195 3351 209 181
BsrGI T'GTAC_A
Cuts at: 416 416
Size: 8936
BssSI C'ACGA_G
Cuts at: 7207 7565 7207
Size: 358 8578
BstAPI GCAn_nnn'nTGC
Cuts at: 703 1390 4122 4386 6313 703
Size: 687 2732 264 1927 3326
Fragments arranged by size:
3326 2732 1927 687 264
BstBI TT'CG_AA
Cuts at: 7297 7297
Size: 8936
BstXI CCAn_nnnn'nTGG
Cuts at: 5028 5028
Size: 8936
BstYI r'GATC_y
Cuts at: 1 1296 2581 4478 4796 5087 5499 5558 5961
Size: 1295 1285 1897 318 291 412 59 403
Cuts at: 5961 6428 6451 6786 7032 8044 8130 8142 8488
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8488 1
Size: 449
Fragments arranged by size:
1897 1295 1285 1012 467 449 412 403
346 335 318 291 246 86 59 23
12
Bsu36I CC'TnA_GG
Cuts at: 1739 2110 3700 5044 1739
Size: 371 1590 1344 5631
Fragments arranged by size:
5631 1590 1344 371
BtgI C'CryG_G
Cuts at: 1928 2174 1928
Size: 246 8690
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6886 7067 7230 6886
Size: 181 163 8592
Fragments arranged by size:
8592 181 163
BtsI GCAGTG_nn'
Cuts at: 6179 6179
Size: 8936
ClaI AT'CG_AT
Cuts at: 1085 1085
Size: 8936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2013 2048 2013
Size: 35 8901
DraI TTT'AAA
Cuts at: 5935 5935
Size: 8936
DraIII CAC_nnn'GTG
Cuts at: 2133 2133
Size: 8936
DrdI GACnn_nn'nnGTC
Cuts at: 6642 7500 6642
Size: 858 8078
EaeI y'GGCC_r
Cuts at: 380 1076 1106 1397 2672 3008 3692 3725 6521
Size: 696 30 291 1275 336 684 33 2796
Cuts at: 6521 6695 7086 7113 8458 380
Size: 174 391 27 1345 858
Fragments arranged by size:
2796 1345 1275 858 696 684 391 336
291 174 33 30 27
EagI C'GGCC_G
Cuts at: 6521 6521
Size: 8936
EarI CTCTTCn'nnn_
Cuts at: 475 1715 1981 2055 2626 2641 2909 2938 3059
Size: 1240 266 74 571 15 268 29 121
Cuts at: 3059 3781 3908 4079 4781 4879 5887 5921 6959
Size: 722 127 171 702 98 1008 34 1038
Cuts at: 6959 7169 475
Size: 210 2242
Fragments arranged by size:
2242 1240 1038 1008 722 702 571 268
266 210 171 127 121 98 74 34
29 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 244 7144 7454 7600 8746 244
Size: 6900 310 146 1146 434
Fragments arranged by size:
6900 1146 434 310 146
EcoICRI GAG'CTC
Cuts at: 1683 5241 5948 1683
Size: 3558 707 4671
Fragments arranged by size:
4671 3558 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 817 1261 1555 1624 1918 2018 2339 2825
Size: 155 444 294 69 294 100 321 486
Cuts at: 2825 2974 3106 3499 3574 3581 3679 3796 4303
Size: 149 132 393 75 7 98 117 507
Cuts at: 4303 4639 4723 4729 4903 5146 5335 5344 5452
Size: 336 84 6 174 243 189 9 108
Cuts at: 5452 5482 5560 5590 5722 5806 5854 6761 7193
Size: 30 78 30 132 84 48 907 432
Cuts at: 7193 7940 8208 662
Size: 747 268 1390
Fragments arranged by size:
1390 907 747 507 486 444 432 393
336 321 294 294 268 243 189 174
155 149 132 132 117 108 100 98
84 84 78 75 69 48 30 30
9 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 796 1720 3661 5167 796
Size: 924 1941 1506 4565
Fragments arranged by size:
4565 1941 1506 924
EcoO109I rG'GnC_Cy
Cuts at: 197 558 1911 3233 3654 4404 5040 5121 6075
Size: 361 1353 1322 421 750 636 81 954
Cuts at: 6075 197
Size: 3058
Fragments arranged by size:
3058 1353 1322 954 750 636 421 361
81
EcoRI G'AATT_C
Cuts at: 5940 5940
Size: 8936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2728 6955 2446
Size: 282 4227 4427
Fragments arranged by size:
4427 4227 282
FspI TGC'GCA
Cuts at: 3649 3950 4804 5647 6717 3649
Size: 301 854 843 1070 5868
Fragments arranged by size:
5868 1070 854 843 301
HaeII r_GCGC'y
Cuts at: 552 3534 3963 5456 6370 6618 7640 8619 552
Size: 2982 429 1493 914 248 1022 979 869
Fragments arranged by size:
2982 1493 1022 979 914 869 429 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 610 642 823 855 948 980 2106
Size: 32 200 32 181 32 93 32 1126
Cuts at: 2106 2138 2148 2180 2622 2654 3060 3092 4165
Size: 32 10 32 442 32 406 32 1073
Cuts at: 4165 4197 5773 5805 6778 6808 6810 6840 378
Size: 32 1576 32 973 30 2 30 2474
Fragments arranged by size:
2474 1576 1126 1073 973 442 406 200
181 93 32 32 32 32 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 486 3177 5975 486
Size: 2691 2798 3447
Fragments arranged by size:
3447 2798 2691
KasI G'GCGC_C
Cuts at: 3959 6366 6614 3959
Size: 2407 248 6281
Fragments arranged by size:
6281 2407 248
KpnI G_GTAC'C
Cuts at: 5956 5956
Size: 8936
MfeI C'AATT_G
Cuts at: 701 3433 701
Size: 2732 6204
MluI A'CGCG_T
Cuts at: 8369 8369
Size: 8936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1403 1914 5816 7607 7791 1403
Size: 511 3902 1791 184 2548
Fragments arranged by size:
3902 2548 1791 511 184
MscI TGG'CCA
Cuts at: 382 1078 1108 1399 2674 3010 6697 382
Size: 696 30 291 1275 336 3687 2621
Fragments arranged by size:
3687 2621 1275 696 336 291 30
MslI CAynn'nnrTG
Cuts at: 398 1597 4142 5096 6925 7052 398
Size: 1199 2545 954 1829 127 2282
Fragments arranged by size:
2545 2282 1829 1199 954 127
MspA1I CmG'CkG
Cuts at: 1455 1542 2202 2475 3691 3927 4311 4324 4383
Size: 87 660 273 1216 236 384 13 59
Cuts at: 4383 4881 5010 5136 5532 6047 6361 6721 7734
Size: 498 129 126 396 515 314 360 1013
Cuts at: 7734 7979 1455
Size: 245 2412
Fragments arranged by size:
2412 1216 1013 660 515 498 396 384
360 314 273 245 236 129 126 87
59 13
NaeI GCC'GGC
Cuts at: 7117 7117
Size: 8936
NarI GG'CG_CC
Cuts at: 3960 6367 6615 3960
Size: 2407 248 6281
Fragments arranged by size:
6281 2407 248
NcoI C'CATG_G
Cuts at: 2174 2174
Size: 8936
NdeI CA'TA_TG
Cuts at: 1038 1038
Size: 8936
NgoMIV G'CCGG_C
Cuts at: 7115 7115
Size: 8936
NsiI A_TGCA'T
Cuts at: 1190 1190
Size: 8936
NspI r_CATG'y
Cuts at: 1596 1734 3034 3715 4120 4336 4519 5989 6489
Size: 138 1300 681 405 216 183 1470 500
Cuts at: 6489 7396 1596
Size: 907 3136
Fragments arranged by size:
3136 1470 1300 907 681 500 405 216
183 138
PasI CC'CwG_GG
Cuts at: 1322 1322
Size: 8936
PciI A'CATG_T
Cuts at: 1592 4332 7392 1592
Size: 2740 3060 3136
Fragments arranged by size:
3136 3060 2740
PflMI CCAn_nnn'nTGG
Cuts at: 1455 1785 2535 4701 5568 1455
Size: 330 750 2166 867 4823
Fragments arranged by size:
4823 2166 867 750 330
PfoI T'CCnGG_A
Cuts at: 1715 3122 5243 5360 1715
Size: 1407 2121 117 5291
Fragments arranged by size:
5291 2121 1407 117
PmeI GTTT'AAAC
Cuts at: 5935 5935
Size: 8936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1405 1437 1627 1659 1717 1749 3619 3651 5225
Size: 32 190 32 58 32 1870 32 1574
Cuts at: 5225 5257 8075 8107 1405
Size: 32 2818 32 2234
Fragments arranged by size:
2818 2234 1870 1574 190 58 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1911 3233 3654 4404 5121 1911
Size: 1322 421 750 717 5726
Fragments arranged by size:
5726 1322 750 717 421
PsiI TTA'TAA
Cuts at: 5759 5759
Size: 8936
PstI C_TGCA'G
Cuts at: 1296 3158 3758 3968 4127 4199 4319 4391 5171
Size: 1862 600 210 159 72 120 72 780
Cuts at: 5171 5507 5983 6668 1296
Size: 336 476 685 3564
Fragments arranged by size:
3564 1862 780 685 600 476 336 210
159 120 72 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8936
PvuII CAG'CTG
Cuts at: 1455 1542 2202 2475 4324 4881 5010 5136 5532
Size: 87 660 273 1849 557 129 126 396
Cuts at: 5532 6361 6721 1455
Size: 829 360 3670
Fragments arranged by size:
3670 1849 829 660 557 396 360 273
129 126 87
RsrII CG'GwC_CG
Cuts at: 7131 7131
Size: 8936
SacI G_AGCT'C
Cuts at: 1685 5243 5950 1685
Size: 3558 707 4671
Fragments arranged by size:
4671 3558 707
SalI G'TCGA_C
Cuts at: 5973 5973
Size: 8936
SapI GCTCTTCn'nnn_
Cuts at: 1981 2055 5887 6959 7169 1981
Size: 74 3832 1072 210 3748
Fragments arranged by size:
3832 3748 1072 210 74
SbfI CC_TGCA'GG
Cuts at: 3158 5983 3158
Size: 2825 6111
ScaI AGT'ACT
Cuts at: 691 1480 8453 691
Size: 789 6973 1174
Fragments arranged by size:
6973 1174 789
SfcI C'TryA_G
Cuts at: 33 1292 1364 2238 3154 3673 3754 3964 4123
Size: 1259 72 874 916 519 81 210 159
Cuts at: 4123 4195 4315 4387 5052 5167 5503 5979 6664
Size: 72 120 72 665 115 336 476 685
Cuts at: 6664 7657 7848 33
Size: 993 191 1121
Fragments arranged by size:
1259 1121 993 916 874 685 665 519
476 336 210 191 159 120 115 81
72 72 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 3511 5395 3511
Size: 1884 7052
SfoI GGC'GCC
Cuts at: 3961 6368 6616 3961
Size: 2407 248 6281
Fragments arranged by size:
6281 2407 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8936
SmaI CCC'GGG
Cuts at: 3511 3547 5958 3511
Size: 36 2411 6489
Fragments arranged by size:
6489 2411 36
SmlI C'TyrA_G
Cuts at: 946 2974 2995 3289 4525 4813 4966 5074 6091
Size: 2028 21 294 1236 288 153 108 1017
Cuts at: 6091 7006 7498 7760 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 2028 1236 1017 915 492 294 288
262 153 108 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8936
SphI G_CATG'C
Cuts at: 3715 4120 4519 5989 6489 3715
Size: 405 399 1470 500 6162
Fragments arranged by size:
6162 1470 500 405 399
SspI AAT'ATT
Cuts at: 5706 5706
Size: 8936
StuI AGG'CCT
Cuts at: 802 2347 4531 802
Size: 1545 2184 5207
Fragments arranged by size:
5207 2184 1545
StyI C'CwwG_G
Cuts at: 1814 2174 2408 3011 3104 3236 3848 4184 4211
Size: 360 234 603 93 132 612 336 27
Cuts at: 4211 4554 5064 6070 6424 1814
Size: 343 510 1006 354 4326
Fragments arranged by size:
4326 1006 612 603 510 360 354 343
336 234 132 93 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 629 2401 5077 5293 6519 6652 7111 7320 629
Size: 1772 2676 216 1226 133 459 209 2245
Fragments arranged by size:
2676 2245 1772 1226 459 216 209 133
TatI w'GTAC_w
Cuts at: 416 689 1478 1616 2570 8451 416
Size: 273 789 138 954 5881 901
Fragments arranged by size:
5881 954 901 789 273 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 269 301 559 591 1528 1560 1711 1743 8453
Size: 32 258 32 937 32 151 32 6710
Cuts at: 8453 8485 269
Size: 32 720
Fragments arranged by size:
6710 937 720 258 151 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2064 3172 6733 2064
Size: 1108 3561 4267
Fragments arranged by size:
4267 3561 1108
XbaI T'CTAG_A
Cuts at: 928 3879 5967 928
Size: 2951 2088 3897
Fragments arranged by size:
3897 2951 2088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 792 2395 4107 4299 5310 5457 6431 792
Size: 1603 1712 192 1011 147 974 3297
Fragments arranged by size:
3297 1712 1603 1011 974 192 147
XmaI C'CCGG_G
Cuts at: 3509 3545 5956 3509
Size: 36 2411 6489
Fragments arranged by size:
6489 2411 36
XmnI GAAnn'nnTTC
Cuts at: 1016 1420 2620 2690 1016
Size: 404 1200 70 7262
Fragments arranged by size:
7262 1200 404 70
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI ApaI AscI AvrII BaeI BmgBI
BmtI BsiWI BssHII BstEII BstZ17I EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI PacI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SwaI XhoI ZraI