Restriction map for
pF1KE2435
(Circular) (Six-base) MAPSORT of: pF1KE2435.seq Check: 639 from: 1 to: 4112
pF1KE2435 4112 bp
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1937 2369 3116 3384 1084
Size: 853 432 747 268 1812
Fragments arranged by size:
1812 853 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AleI CACnn'nnGTG
Cuts at: 622 622
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 255 1417 2984 255
Size: 1162 1567 1383
Fragments arranged by size:
1567 1383 1162
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 225 1116 3465 3721 225
Size: 891 2349 256 616
Fragments arranged by size:
2349 891 616 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 1128 1542 1790 1825 1128
Size: 414 248 35 3415
Fragments arranged by size:
3415 414 248 35
BanII G_rGCy'C
Cuts at: 1053 1126 2156 1053
Size: 73 1030 3009
Fragments arranged by size:
3009 1030 73
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbvCI CC'TCA_GC
Cuts at: 445 445
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglII A'GATC_T
Cuts at: 524 669 1627 3664 524
Size: 145 958 2037 972
Fragments arranged by size:
2037 972 958 145
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 911 1737 1830 2886 911
Size: 826 93 1056 2137
Fragments arranged by size:
2137 1056 826 93
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 501 844 199
Size: 302 343 3467
Fragments arranged by size:
3467 343 302
Bpu10I CC'TnA_GC
Cuts at: 445 445
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1288 2167 2659 2957 829
Size: 459 879 492 298 1984
Fragments arranged by size:
1984 879 492 459 298
BsaI GGTCTCn'nnnn_
Cuts at: 519 3816 519
Size: 3297 815
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1035 1035
Size: 4112
BseYI C'CCAG_C
Cuts at: 509 817 1537 2295 2872 3368 509
Size: 308 720 758 577 496 1253
Fragments arranged by size:
1253 758 720 577 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 552
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 911 1053 1126 1737 1830 1904 2094 2156 2886
Size: 142 73 611 93 74 190 62 730
Cuts at: 2886 911
Size: 2137
Fragments arranged by size:
2137 730 611 190 142 93 74 73
62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 632 3288 632
Size: 2656 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1014 2024 1014
Size: 1010 3102
BsrFI r'CCGG_y
Cuts at: 296 2110 2291 2500 296
Size: 1814 181 209 1908
Fragments arranged by size:
1908 1814 209 181
BssSI C'ACGA_G
Cuts at: 809 2383 2741 809
Size: 1574 358 2180
Fragments arranged by size:
2180 1574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 315 1489 315
Size: 1174 2938
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstYI r'GATC_y
Cuts at: 1 524 669 1137 1604 1627 1962 2208 3220
Size: 523 145 468 467 23 335 246 1012
Cuts at: 3220 3306 3318 3664 1
Size: 86 12 346 449
Fragments arranged by size:
1012 523 468 467 449 346 335 246
145 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 194 1355 194
Size: 1161 2951
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 1697 1871 2262 2289 3634 1697
Size: 174 391 27 1345 2175
Fragments arranged by size:
2175 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 238 700 2135 2345 238
Size: 462 1435 210 2005
Fragments arranged by size:
2005 1435 462 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 501 844 1084 1937 2369 3116 3384 199
Size: 302 343 240 853 432 747 268 927
Fragments arranged by size:
927 853 747 432 343 302 268 240
EcoO109I rG'GnC_Cy
Cuts at: 1251 1251
Size: 4112
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
EcoRV GAT'ATC
Cuts at: 881 881
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 654 686 1954 1984 1986 2016 105
Size: 32 517 32 1268 30 2 30 2201
Fragments arranged by size:
2201 1268 517 32 32 30 30 2
HincII GTy'rAC
Cuts at: 113 1151 113
Size: 1038 3074
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 394 622 904 2101 2228 394
Size: 228 282 1197 127 2278
Fragments arranged by size:
2278 1197 282 228 127
MspA1I CmG'CkG
Cuts at: 1223 1537 1897 2910 3155 1223
Size: 314 360 1013 245 2180
Fragments arranged by size:
2180 1013 360 314 245
NaeI GCC'GGC
Cuts at: 298 2293 298
Size: 1995 2117
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NgoMIV G'CCGG_C
Cuts at: 296 2291 296
Size: 1995 2117
NspI r_CATG'y
Cuts at: 988 1165 1665 2572 988
Size: 177 500 907 2528
Fragments arranged by size:
2528 907 500 177
PasI CC'CwG_GG
Cuts at: 765 765
Size: 4112
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 428 428
Size: 4112
PfoI T'CCnGG_A
Cuts at: 176 517 1093 176
Size: 341 576 3195
Fragments arranged by size:
3195 576 341
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 364 396 364
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 1155 1840 2833 3024 33
Size: 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 844 1267 2182 2674 2936 844
Size: 423 915 492 262 2020
Fragments arranged by size:
2020 915 492 423 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 988 1165 1665 988
Size: 177 500 3435
Fragments arranged by size:
3435 500 177
StyI C'CwwG_G
Cuts at: 1246 1600 1246
Size: 354 3758
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 154 3627 154
Size: 3473 639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI