Restriction map for
pF1KE2430
(Circular) (Six-base) MAPSORT of: pF1KE2430.seq Check: 4237 from: 1 to: 4712
pF1KE2430 4712 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AatII G_ACGT'C
Cuts at: 287 287
Size: 4712
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1138 1571 2537 2969 3716 3984 826
Size: 312 433 966 432 747 268 1554
Fragments arranged by size:
1554 966 747 433 432 312 268
AflIII A'CryG_T
Cuts at: 1232 2064 3168 4145 1232
Size: 832 1104 977 1799
Fragments arranged by size:
1799 1104 977 832
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AleI CACnn'nnGTG
Cuts at: 1687 1687
Size: 4712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 920 920
Size: 4712
AlwNI CAG_nnn'CTG
Cuts at: 375 2017 3584 375
Size: 1642 1567 1503
Fragments arranged by size:
1642 1567 1503
ApaI G_GGCC'C
Cuts at: 1699 1699
Size: 4712
ApaLI G'TGCA_C
Cuts at: 378 3482 378
Size: 3104 1608
ApoI r'AATT_y
Cuts at: 1716 4065 4321 1716
Size: 2349 256 2107
Fragments arranged by size:
2349 2107 256
AseI AT'TA_AT
Cuts at: 20 655 20
Size: 635 4077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 137 216 1732 137
Size: 79 1516 3117
Fragments arranged by size:
3117 1516 79
AvrII C'CTAG_G
Cuts at: 270 270
Size: 4712
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 204 220 545 1728 2142 2390 2425 204
Size: 16 325 1183 414 248 35 2491
Fragments arranged by size:
2491 1183 414 325 248 35 16
BanII G_rGCy'C
Cuts at: 217 839 974 1148 1699 1726 2756 217
Size: 622 135 174 551 27 1030 2173
Fragments arranged by size:
2173 1030 622 551 174 135 27
BbeI G_GCGC'C
Cuts at: 208 224 2146 2394 208
Size: 16 1922 248 2526
Fragments arranged by size:
2526 1922 248 16
BbsI GAAGACnn'nnnn_
Cuts at: 243 243
Size: 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 728 694
Size: 34 4678
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglII A'GATC_T
Cuts at: 2227 4264 2227
Size: 2037 2675
BlpI GC'TnA_GC
Cuts at: 1824 1824
Size: 4712
Bme1580I G_kGCm'C
Cuts at: 382 1699 2337 2430 3486 382
Size: 1317 638 93 1056 1608
Fragments arranged by size:
1608 1317 1056 638 93
BmgBI CAC'GTC
Cuts at: 1233 1233
Size: 4712
BmrI ACTGGGnnnn_n'
Cuts at: 1694 1924 2005 2340 4484 1694
Size: 230 81 335 2144 1922
Fragments arranged by size:
2144 1922 335 230 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1512 3079 1512
Size: 1567 3145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1325 805
Size: 520 4192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2767 3259 3557 1888
Size: 879 492 298 3043
Fragments arranged by size:
3043 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4416 4416
Size: 4712
BsaAI yAC'GTr
Cuts at: 2065 2065
Size: 4712
BsaBI GATnn'nnATC
Cuts at: 6 1538 2226 4263 6
Size: 1532 688 2037 455
Fragments arranged by size:
2037 1532 688 455
BsaHI Gr'CG_yC
Cuts at: 205 221 284 1082 2143 2391 3093 4589 205
Size: 16 63 798 1061 248 702 1496 328
Fragments arranged by size:
1496 1061 798 702 328 248 63 16
BsaWI w'CCGG_w
Cuts at: 560 1901 2422 3100 3374 3521 3932 560
Size: 1341 521 678 274 147 411 1340
Fragments arranged by size:
1341 1340 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 1417 154
Size: 1263 3449
BseYI C'CCAG_C
Cuts at: 463 497 2137 2895 3472 3968 463
Size: 34 1640 758 577 496 1207
Fragments arranged by size:
1640 1207 758 577 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 1637
Size: 4712
BsiEI CG_ry'CG
Cuts at: 104 238 338 2254 2300 3508 104
Size: 134 100 1916 46 1208 1308
Fragments arranged by size:
1916 1308 1208 134 100 46
BsiHKAI G_wGCw'C
Cuts at: 350 382 687 1148 1726 2504 2694 3486 350
Size: 32 305 461 578 778 190 792 1576
Fragments arranged by size:
1576 792 778 578 461 305 190 32
BsmI GAATG_Cn'
Cuts at: 574 574
Size: 4712
BsmBI CGTCTCn'nnnn_
Cuts at: 1475 1475
Size: 4712
Bsp1286I G_dGCh'C
Cuts at: 217 350 382 687 839 974 1148 1699 1726
Size: 133 32 305 152 135 174 551 27
Cuts at: 1726 2337 2430 2504 2694 2756 3486 217
Size: 611 93 74 190 62 730 1443
Fragments arranged by size:
1443 730 611 551 305 190 174 152
135 133 93 74 62 32 27
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 1178 3888 1178
Size: 2710 2002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 505 1762 1976 2278 2659 505
Size: 1257 214 302 381 2558
Fragments arranged by size:
2558 1257 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3004 3058 4542 4707 50
Size: 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2624 2624
Size: 4712
BsrFI r'CCGG_y
Cuts at: 233 337 2710 2891 3100 233
Size: 104 2373 181 209 1845
Fragments arranged by size:
2373 1845 209 181 104
BsrGI T'GTAC_A
Cuts at: 649 649
Size: 4712
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 2089 2089
Size: 4712
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstYI r'GATC_y
Cuts at: 1 419 1737 2204 2227 2562 2808 3820 3906
Size: 418 1318 467 23 335 246 1012 86
Cuts at: 3906 3918 4264 1
Size: 12 346 449
Fragments arranged by size:
1318 1012 467 449 418 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 851 851
Size: 4712
BtgI C'CryG_G
Cuts at: 107 294 107
Size: 187 4525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 947 972 1955 947
Size: 25 983 3704
Fragments arranged by size:
3704 983 25
DraI TTT'AAA
Cuts at: 959 1711 959
Size: 752 3960
DraIII CAC_nnn'GTG
Cuts at: 682 682
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3276 2418
Size: 858 3854
EaeI y'GGCC_r
Cuts at: 235 335 408 1187 2297 2471 2862 2889 4234
Size: 100 73 779 1110 174 391 27 1345
Cuts at: 4234 235
Size: 713
Fragments arranged by size:
1345 1110 779 713 391 174 100 73
27
EagI C'GGCC_G
Cuts at: 235 335 2297 235
Size: 100 1962 2650
Fragments arranged by size:
2650 1962 100
EarI CTCTTCn'nnn_
Cuts at: 1317 1362 1367 2735 2945 1317
Size: 45 5 1368 210 3084
Fragments arranged by size:
3084 1368 210 45 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2920 3230 3376 4522 2920
Size: 310 146 1146 3110
Fragments arranged by size:
3110 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1146 1724 1146
Size: 578 4134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 826 1138 1325 1571 2537 2969 3716 3984
Size: 21 312 187 246 966 432 747 268
Cuts at: 3984 805
Size: 1533
Fragments arranged by size:
1533 966 747 432 312 268 246 187
21
EcoO109I rG'GnC_Cy
Cuts at: 142 846 1545 1659 1695 1696 1851 142
Size: 704 699 114 36 1 155 3003
Fragments arranged by size:
3003 704 699 155 114 36 1
EcoRI G'AATT_C
Cuts at: 1716 1716
Size: 4712
EcoRV GAT'ATC
Cuts at: 610 610
Size: 4712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 589 2731 589
Size: 2142 2570
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 208 224 2146 2394 3416 4395 208
Size: 16 1922 248 1022 979 525
Fragments arranged by size:
1922 1022 979 525 248 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2554 2584 2586 2616 2554
Size: 30 2 30 4650
Fragments arranged by size:
4650 30 30 2
HincII GTy'rAC
Cuts at: 1751 1751
Size: 4712
KasI G'GCGC_C
Cuts at: 204 220 2142 2390 204
Size: 16 1922 248 2526
Fragments arranged by size:
2526 1922 248 16
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 516 601 3383 3567 516
Size: 85 2782 184 1661
Fragments arranged by size:
2782 1661 184 85
MscI TGG'CCA
Cuts at: 1189 2473 1189
Size: 1284 3428
MslI CAynn'nnrTG
Cuts at: 902 1202 1687 2701 2828 902
Size: 300 485 1014 127 2786
Fragments arranged by size:
2786 1014 485 300 127
MspA1I CmG'CkG
Cuts at: 183 375 399 467 1667 1823 2137 2497 3510
Size: 192 24 68 1200 156 314 360 1013
Cuts at: 3510 3755 183
Size: 245 1140
Fragments arranged by size:
1200 1140 1013 360 314 245 192 156
68 24
NaeI GCC'GGC
Cuts at: 235 339 2893 235
Size: 104 2554 2054
Fragments arranged by size:
2554 2054 104
NarI GG'CG_CC
Cuts at: 205 221 2143 2391 205
Size: 16 1922 248 2526
Fragments arranged by size:
2526 1922 248 16
NcoI C'CATG_G
Cuts at: 107 107
Size: 4712
NdeI CA'TA_TG
Cuts at: 530 870 530
Size: 340 4372
NgoMIV G'CCGG_C
Cuts at: 233 337 2891 233
Size: 104 2554 2054
Fragments arranged by size:
2554 2054 104
NspI r_CATG'y
Cuts at: 1765 2265 3172 1765
Size: 500 907 3305
Fragments arranged by size:
3305 907 500
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4712
PflMI CCAn_nnn'nTGG
Cuts at: 1447 1447
Size: 4712
PfoI T'CCnGG_A
Cuts at: 350 350
Size: 4712
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 846 1545 1659 846
Size: 699 114 3899
Fragments arranged by size:
3899 699 114
PspOMI G'GGCC_C
Cuts at: 1695 1695
Size: 4712
PstI C_TGCA'G
Cuts at: 954 1759 2444 954
Size: 805 685 3222
Fragments arranged by size:
3222 805 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 399 467 2137 2497 399
Size: 68 1670 360 2614
Fragments arranged by size:
2614 1670 360 68
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 1148 1726 1148
Size: 578 4134
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SanDI GG'GwC_CC
Cuts at: 1545 1545
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 4229 4229
Size: 4712
SexAI A'CCwGG_T
Cuts at: 245 245
Size: 4712
SfcI C'TryA_G
Cuts at: 33 764 950 1001 1612 1755 2440 3433 3624
Size: 731 186 51 611 143 685 993 191
Cuts at: 3624 33
Size: 1121
Fragments arranged by size:
1121 993 731 685 611 191 186 143
51
SfoI GGC'GCC
Cuts at: 206 222 2144 2392 206
Size: 16 1922 248 2526
Fragments arranged by size:
2526 1922 248 16
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1734 1734
Size: 4712
SmlI C'TyrA_G
Cuts at: 1867 2782 3274 3536 1867
Size: 915 492 262 3043
Fragments arranged by size:
3043 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
SspI AAT'ATT
Cuts at: 1290 1372 1290
Size: 82 4630
StyI C'CwwG_G
Cuts at: 107 270 1676 1699 1846 2200 107
Size: 163 1406 23 147 354 2619
Fragments arranged by size:
2619 1406 354 163 147 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2295 2428 2887 3096 2295
Size: 133 459 209 3911
Fragments arranged by size:
3911 459 209 133
TatI w'GTAC_w
Cuts at: 649 1643 4227 649
Size: 994 2584 1134
Fragments arranged by size:
2584 1134 994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 4680
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 403 1332 2207 403
Size: 929 875 2908
Fragments arranged by size:
2908 929 875
XmaI C'CCGG_G
Cuts at: 1732 1732
Size: 4712
XmnI GAAnn'nnTTC
Cuts at: 572 572
Size: 4712
ZraI GAC'GTC
Cuts at: 285 285
Size: 4712
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI BaeI BbvCI
BfrBI BglI BmtI Bpu10I BsaXI BsiWI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI