Restriction map for
pF1KE2428
(Circular) (Six-base) MAPSORT of: pF1KE2428.seq Check: 5743 from: 1 to: 4160
pF1KE2428 4160 bp
With 182 enzymes: SgfI *
November 16, 2012 10:47 ..
AccI GT'mk_AC
Cuts at: 473 1055 1198 473
Size: 582 143 3435
Fragments arranged by size:
3435 582 143
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 514 1985 2417 3164 3432 454
Size: 60 1471 432 747 268 1182
Fragments arranged by size:
1471 1182 747 432 268 60
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AleI CACnn'nnGTG
Cuts at: 676 676
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 996 1465 3032 996
Size: 469 1567 2124
Fragments arranged by size:
2124 1567 469
ApaI G_GGCC'C
Cuts at: 484 1007 484
Size: 523 3637
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 1117 1164 3513 3769 1117
Size: 47 2349 256 1508
Fragments arranged by size:
2349 1508 256 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 1006 1016 1180 1006
Size: 10 164 3986
Fragments arranged by size:
3986 164 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 651 684 651
Size: 33 4127
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 384 1176 1590 1838 1873 384
Size: 792 414 248 35 2671
Fragments arranged by size:
2671 792 414 248 35
BanII G_rGCy'C
Cuts at: 154 484 1007 1174 2204 154
Size: 330 523 167 1030 2110
Fragments arranged by size:
2110 1030 523 330 167
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4160
BbvCI CC'TCA_GC
Cuts at: 929 929
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 890 1680 890
Size: 790 3370
BglI GCCn_nnn'nGGC
Cuts at: 193 736 193
Size: 543 3617
BglII A'GATC_T
Cuts at: 407 701 1675 3712 407
Size: 294 974 2037 855
Fragments arranged by size:
2037 974 855 294
BlpI GC'TnA_GC
Cuts at: 153 1272 153
Size: 1119 3041
Bme1580I G_kGCm'C
Cuts at: 308 389 484 1007 1785 1878 2934 308
Size: 81 95 523 778 93 1056 1534
Fragments arranged by size:
1534 1056 778 523 95 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 156 1372 1453 1788 3932 156
Size: 1216 81 335 2144 384
Fragments arranged by size:
2144 1216 384 335 81
BmtI G_CTAG'C
Cuts at: 116 116
Size: 4160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1021 988
Size: 33 4127
Bpu10I CC'TnA_GC
Cuts at: 929 929
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1336 2215 2707 3005 406
Size: 930 879 492 298 1561
Fragments arranged by size:
1561 930 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 917 1349 1870 2548 2822 2969 3380 917
Size: 432 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 432 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 961 991 961
Size: 30 4130
BseYI C'CCAG_C
Cuts at: 188 795 1585 2343 2920 3416 188
Size: 607 790 758 577 496 932
Fragments arranged by size:
932 790 758 607 577 496
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 154 308 389 484 1007 1174 1785 1878 1952
Size: 154 81 95 523 167 611 93 74
Cuts at: 1952 2142 2204 2934 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 730 611 523 190 167 154 95
93 81 74 62
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 907 1210 1424 1726 2107 305
Size: 602 303 214 302 381 2358
Fragments arranged by size:
2358 602 381 303 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 560 2072 186
Size: 374 1512 2274
Fragments arranged by size:
2274 1512 374
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BsrGI T'GTAC_A
Cuts at: 1082 1082
Size: 4160
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 312 361 996 1537 312
Size: 49 635 541 2935
Fragments arranged by size:
2935 635 541 49
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstEII G'GTnAC_C
Cuts at: 213 213
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 171 573 864 171
Size: 402 291 3467
Fragments arranged by size:
3467 402 291
BstYI r'GATC_y
Cuts at: 1 407 701 1185 1652 1675 2010 2256 3268
Size: 406 294 484 467 23 335 246 1012
Cuts at: 3268 3354 3366 3712 1
Size: 86 12 346 449
Fragments arranged by size:
1012 484 467 449 406 346 335 294
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 208 731 107
Size: 101 523 3536
Fragments arranged by size:
3536 523 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 2110 2291 2454 144
Size: 1966 181 163 1850
Fragments arranged by size:
1966 1850 181 163
BtsI GCAGTG_nn'
Cuts at: 791 843 1125 1403 791
Size: 52 282 278 3548
Fragments arranged by size:
3548 282 278 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 308 343 308
Size: 35 4125
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 728 1745 1919 2310 2337 3682 728
Size: 1017 174 391 27 1345 1206
Fragments arranged by size:
1345 1206 1017 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 835 2183 2393 835
Size: 1348 210 2602
Fragments arranged by size:
2602 1348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 927 2368 2678 2824 3970 927
Size: 1441 310 146 1146 1117
Fragments arranged by size:
1441 1146 1117 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 514 988 1021 1985 2417 3164 3432 454
Size: 60 474 33 964 432 747 268 1182
Fragments arranged by size:
1182 964 747 474 432 268 60 33
EcoO109I rG'GnC_Cy
Cuts at: 480 481 615 1003 1047 1299 480
Size: 1 134 388 44 252 3341
Fragments arranged by size:
3341 388 252 134 44 1
EcoRI G'AATT_C
Cuts at: 1117 1164 1117
Size: 47 4113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1083 2179 1083
Size: 1096 3064
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 525 1594 1842 2864 3843 525
Size: 1069 248 1022 979 842
Fragments arranged by size:
1069 1022 979 842 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2002 2032 2034 2064 2002
Size: 30 2 30 4098
Fragments arranged by size:
4098 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3015 2831
Size: 184 3976
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 284 535 541 676 808 1078 2149 2276 284
Size: 251 6 135 132 270 1071 127 2168
Fragments arranged by size:
2168 1071 270 251 135 132 127 6
MspA1I CmG'CkG
Cuts at: 192 966 1271 1585 1945 2958 3203 192
Size: 774 305 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 774 360 314 305 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 107
Size: 4160
NdeI CA'TA_TG
Cuts at: 1151 1151
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NheI G'CTAG_C
Cuts at: 112 112
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 2620 1213
Size: 500 907 2753
Fragments arranged by size:
2753 907 500
PasI CC'CwG_GG
Cuts at: 624 624
Size: 4160
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 1110 1110
Size: 4160
PfoI T'CCnGG_A
Cuts at: 139 139
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 136 168 3299 3331 136
Size: 32 3131 32 965
Fragments arranged by size:
3131 965 32 32
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 4160
PspOMI G'GGCC_C
Cuts at: 480 1003 480
Size: 523 3637
PstI C_TGCA'G
Cuts at: 357 838 1001 1136 1207 1892 357
Size: 481 163 135 71 685 2625
Fragments arranged by size:
2625 685 481 163 135 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 192 966 1585 1945 192
Size: 774 619 360 2407
Fragments arranged by size:
2407 774 619 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 835 2183 2393 835
Size: 1348 210 2602
Fragments arranged by size:
2602 1348 210
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 353 834 997 1132 1203 1888 2881 3072
Size: 320 481 163 135 71 685 993 191
Cuts at: 3072 33
Size: 1121
Fragments arranged by size:
1121 993 685 481 320 191 163 135
71
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1018 1182 1018
Size: 164 3996
SmlI C'TyrA_G
Cuts at: 421 1315 2230 2722 2984 421
Size: 894 915 492 262 1597
Fragments arranged by size:
1597 915 894 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StuI AGG'CCT
Cuts at: 934 934
Size: 4160
StyI C'CwwG_G
Cuts at: 107 776 1294 1648 107
Size: 669 518 354 2619
Fragments arranged by size:
2619 669 518 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 1102 1743 1876 2335 2544 163
Size: 939 641 133 459 209 1779
Fragments arranged by size:
1779 939 641 459 209 133
TatI w'GTAC_w
Cuts at: 1082 3675 1082
Size: 2593 1567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 647 679 3677 3709 647
Size: 32 2998 32 1098
Fragments arranged by size:
2998 1098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 963 1655 963
Size: 692 3468
XmaI C'CCGG_G
Cuts at: 1016 1180 1016
Size: 164 3996
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BcgI BfrBI BmgBI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI