Restriction map for pF1KE2407
 (Circular) (Six-base) MAPSORT of: pF1KE2407.seq  Check: 751  from: 1  to: 7685



pF1KE2407  7685 bp

 With 182 enzymes: SgfI * 

                        May 11, 2012 13:46  ..

AccI GT'mk_AC

Cuts at:    282    3597    4723     282 
   Size:       3315    1126    3244

  Fragments arranged by size: 

                3315    3244    1126
 
Acc65I G'GTAC_C

Cuts at:   4701    4701 
   Size:       7685
 
AclI AA'CG_TT

Cuts at:   3609    7525    3609 
   Size:       3916    3769
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     811     897    1586    1673    2225    2531    2843    3590 
   Size:        486      86     689      87     552     306     312     747

Cuts at:   3590    4125    5510    5942    6689    6957     325 
   Size:        535    1385     432     747     268    1053

  Fragments arranged by size: 

                1385    1053     747     747     689     552     535     486
                 432     312     306     268      87      86
 
AflII C'TTAA_G

Cuts at:   1327    2035    1327 
   Size:        708    6977
 
AflIII A'CryG_T

Cuts at:    561    5037    6141    7118     561 
   Size:       4476    1104     977    1128

  Fragments arranged by size: 

                4476    1128    1104     977
 
AgeI A'CCGG_T

Cuts at:   6073    6073 
   Size:       7685
 
AhdI GACnn_n'nnGTC

Cuts at:   4274    4618    4274 
   Size:        344    7341
 
AleI CACnn'nnGTG

Cuts at:   1822    1822 
   Size:       7685
 
AlwNI CAG_nnn'CTG

Cuts at:   1696    4990    6557    1696 
   Size:       3294    1567    2824

  Fragments arranged by size: 

                3294    2824    1567
 
ApaLI G'TGCA_C

Cuts at:   2801    6455    2801 
   Size:       3654    4031
 
ApoI r'AATT_y

Cuts at:    416    2009    2097    2275    2830    4689    7038    7294     416 
   Size:       1593      88     178     555    1859    2349     256     807

  Fragments arranged by size: 

                2349    1859    1593     807     555     256     178      88
            
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7685
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7685
 
AvaI C'yCGr_G

Cuts at:   4705    4705 
   Size:       7685
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    280     313     280 
   Size:         33    7652
 
BamHI G'GATC_C

Cuts at:   4710    4710 
   Size:       7685
 
BanI G'GyrC_C

Cuts at:   4701    5115    5363    5398    4701 
   Size:        414     248      35    6988

  Fragments arranged by size: 

                6988     414     248      35
 
BanII G_rGCy'C

Cuts at:   2378    4699    5729    2378 
   Size:       2321    1030    4334

  Fragments arranged by size: 

                4334    2321    1030
 
BbeI G_GCGC'C

Cuts at:   5119    5367    5119 
   Size:        248    7437
 
BbsI GAAGACnn'nnnn_

Cuts at:   2519    3116    3624    3674    3810    2519 
   Size:        597     508      50     136    6394

  Fragments arranged by size: 

                6394     597     508     136      50
 
BbvCI CC'TCA_GC

Cuts at:   2852    2852 
   Size:       7685
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    7651
 
BciVI GTATCCnnnnn_n'

Cuts at:   2586    5583    6344    7685    2586 
   Size:       2997     761    1341    2586

  Fragments arranged by size: 

                2997    2586    1341     761
 
BclI T'GATC_A

Cuts at:   5205    5205 
   Size:       7685
 
BfrBI ATG'CAT

Cuts at:   1417    2899    1417 
   Size:       1482    6203
 
BglII A'GATC_T

Cuts at:    203    2887    4177    5200    7237     203 
   Size:       2684    1290    1023    2037     651

  Fragments arranged by size: 

                2684    2037    1290    1023     651
 
BlpI GC'TnA_GC

Cuts at:   4797    4797 
   Size:       7685
 
Bme1580I G_kGCm'C

Cuts at:   2805    5310    5403    6459    2805 
   Size:       2505      93    1056    4031

  Fragments arranged by size: 

                4031    2505    1056      93
 
BmgBI CAC'GTC

Cuts at:   4620    4620 
   Size:       7685
 
BmrI ACTGGGnnnn_n'

Cuts at:   1787    4282    4622    4897    4978    5313    7457    1787 
   Size:       2495     340     275      81     335    2144    2015

  Fragments arranged by size: 

                2495    2144    2015     340     335     275      81
 
BmtI G_CTAG'C

Cuts at:   2812    2812 
   Size:       7685
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3383    3385    6052    3383 
   Size:          2    2667    5016

  Fragments arranged by size: 

                5016    2667       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2609    3048    4235    2609 
   Size:        439    1187    6059

  Fragments arranged by size: 

                6059    1187     439
 
Bpu10I CC'TnA_GC

Cuts at:   2852    2852 
   Size:       7685
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    331    2546    3541    3752    3843    4861    5740    6232    6530 
   Size:       2215     995     211      91    1018     879     492     298

Cuts at:   6530     331 
   Size:       1486

  Fragments arranged by size: 

                2215    1486    1018     995     879     492     298     211
                  91
 
BsaI GGTCTCn'nnnn_

Cuts at:    933    3630    7389     933 
   Size:       2697    3759    1229

  Fragments arranged by size: 

                3759    2697    1229
 
BsaAI yAC'GTr

Cuts at:   1252    2638    5038    1252 
   Size:       1386    2400    3899

  Fragments arranged by size: 

                3899    2400    1386
 
BsaBI GATnn'nnATC

Cuts at:      6    5199    7236       6 
   Size:       5193    2037     455

  Fragments arranged by size: 

                5193    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   5116    5364    6066    7562    5116 
   Size:        248     702    1496    5239

  Fragments arranged by size: 

                5239    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   4874    5395    6073    6347    6494    6905    4874 
   Size:        521     678     274     147     411    5654

  Fragments arranged by size: 

                5654     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   3171    3201    3721    3751    4190    4220    3171 
   Size:         30     520      30     439      30    6636

  Fragments arranged by size: 

                6636     520     439      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1754    3615    3742    3965    4316    1754 
   Size:       1861     127     223     351    5123

  Fragments arranged by size: 

                5123    1861     351     223     127
 
BseYI C'CCAG_C

Cuts at:   5110    5868    6445    6941    5110 
   Size:        758     577     496    5854

  Fragments arranged by size: 

                5854     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1635    1660    2671    2942    1635 
   Size:         25    1011     271    6378

  Fragments arranged by size: 

                6378    1011     271      25
 
BsiEI CG_ry'CG

Cuts at:    104    2436    5227    5273    6481     104 
   Size:       2332    2791      46    1208    1308

  Fragments arranged by size: 

                2791    2332    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2378    2805    3916    4699    5477    5667    6459    2378 
   Size:        427    1111     783     778     190     792    3604

  Fragments arranged by size: 

                3604    1111     792     783     778     427     190
 
BsmI GAATG_Cn'

Cuts at:   3459    3459 
   Size:       7685
 
Bsp1286I G_dGCh'C

Cuts at:   2378    2805    3916    4699    5310    5403    5477    5667    5729 
   Size:        427    1111     783     611      93      74     190      62

Cuts at:   5729    6459    2378 
   Size:        730    3604

  Fragments arranged by size: 

                3604    1111     783     730     611     427     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   6905    6905 
   Size:       7685
 
BspHI T'CATG_A

Cuts at:   4308    6861    4308 
   Size:       2553    5132
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    167    4735    4949    5251    5632     167 
   Size:       4568     214     302     381    2220

  Fragments arranged by size: 

                4568    2220     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3288    5977    6031    7515    7680      50 
   Size:       3238    2689      54    1484     165      55

  Fragments arranged by size: 

                3238    2689    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3260    4148    4430    5597    3260 
   Size:        888     282    1167    5348

  Fragments arranged by size: 

                5348    1167     888     282
 
BsrFI r'CCGG_y

Cuts at:   4656    5683    5864    6073    4656 
   Size:       1027     181     209    6268

  Fragments arranged by size: 

                6268    1027     209     181
 
BsrGI T'GTAC_A

Cuts at:   2553    2553 
   Size:       7685
 
BssSI C'ACGA_G

Cuts at:    767    5956    6314     767 
   Size:       5189     358    2138

  Fragments arranged by size: 

                5189    2138     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   5062    5062 
   Size:       7685
 
BstBI TT'CG_AA

Cuts at:   4519    6046    4519 
   Size:       1527    6158
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2684    4030    4635    2684 
   Size:       1346     605    5734

  Fragments arranged by size: 

                5734    1346     605
 
BstYI r'GATC_y

Cuts at:      1     203    1638    2565    2887    4177    4710    5177    5200 
   Size:        202    1435     927     322    1290     533     467      23

Cuts at:   5200    5535    5781    6793    6879    6891    7237       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1435    1290    1012     927     533     467     449     346
                 335     322     246     202      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2570    2570 
   Size:       7685
 
BtgI C'CryG_G

Cuts at:    152     152 
   Size:       7685
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3500    3719    4244    5635    5816    5979    3500 
   Size:        219     525    1391     181     163    5206

  Fragments arranged by size: 

                5206    1391     525     219     181     163
 
BtsI GCAGTG_nn'

Cuts at:    511    3240    4036    4928     511 
   Size:       2729     796     892    3268

  Fragments arranged by size: 

                3268    2729     892     796
 
ClaI AT'CG_AT

Cuts at:    450     450 
   Size:       7685
 
DraI TTT'AAA

Cuts at:   2271    4684    2271 
   Size:       2413    5272
 
DraIII CAC_nnn'GTG

Cuts at:   1519    1519 
   Size:       7685
 
DrdI GACnn_nn'nnGTC

Cuts at:   5391    6249    5391 
   Size:        858    6827
 
EaeI y'GGCC_r

Cuts at:   3867    4290    5270    5444    5835    5862    7207    3867 
   Size:        423     980     174     391      27    1345    4345

  Fragments arranged by size: 

                4345    1345     980     423     391     174      27
 
EagI C'GGCC_G

Cuts at:   5270    5270 
   Size:       7685
 
EarI CTCTTCn'nnn_

Cuts at:    927    1175    1568    2483    2819    3113    3728    3767    3855 
   Size:        248     393     915     336     294     615      39      88

Cuts at:   3855    5708    5918     927 
   Size:       1853     210    2694

  Fragments arranged by size: 

                2694    1853     915     615     393     336     294     248
                 210      88      39
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    199    5893    6203    6349    7495     199 
   Size:       5694     310     146    1146     389

  Fragments arranged by size: 

                5694    1146     389     310     146
 
EcoICRI GAG'CTC

Cuts at:   2376    4697    2376 
   Size:       2321    5364
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    325     811     897    1586    1673    2225    2531    2609    2843 
   Size:        486      86     689      87     552     306      78     234

Cuts at:   2843    3048    3590    4125    4235    5510    5942    6689    6957 
   Size:        205     542     535     110    1275     432     747     268

Cuts at:   6957     325 
   Size:       1053

  Fragments arranged by size: 

                1275    1053     747     689     552     542     535     486
                 432     306     268     234     205     110      87      86
                  78
 
EcoO109I rG'GnC_Cy

Cuts at:   4398    4824    4398 
   Size:        426    7259
 
EcoRI G'AATT_C

Cuts at:   2009    4689    2009 
   Size:       2680    5005
 
EcoRV GAT'ATC

Cuts at:    429    1729     429 
   Size:       1300    6385
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    647    1231    2050    3181    3325    3418    3911    5704     647 
   Size:        584     819    1131     144      93     493    1793    2628

  Fragments arranged by size: 

                2628    1793    1131     819     584     493     144      93
            
 
FspI TGC'GCA

Cuts at:   5466    5466 
   Size:       7685
 
HaeII r_GCGC'y

Cuts at:   5119    5367    6389    7368    5119 
   Size:        248    1022     979    5436

  Fragments arranged by size: 

                5436    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    443     475    1664    1690    1696    1722    2209    2241    3539 
   Size:         32    1189      26       6      26     487      32    1298

Cuts at:   3539    3571    4204    4236    4260    4292    4604    4636    5527 
   Size:         32     633      32      24      32     312      32     891

Cuts at:   5527    5557    5559    5589     443 
   Size:         30       2      30    2539

  Fragments arranged by size: 

                2539    1298    1189     891     633     487     312      32
                  32      32      32      32      32      30      30      26
                  26      24       6       2
 
HincII GTy'rAC

Cuts at:    567    3598    4724     567 
   Size:       3031    1126    3528

  Fragments arranged by size: 

                3528    3031    1126
 
HindIII A'AGCT_T

Cuts at:    781    4606     781 
   Size:       3825    3860
 
HpaI GTT'AAC

Cuts at:    567     567 
   Size:       7685
 
KasI G'GCGC_C

Cuts at:   5115    5363    5115 
   Size:        248    7437
 
KpnI G_GTAC'C

Cuts at:   4705    4705 
   Size:       7685
 
MfeI C'AATT_G

Cuts at:    659     659 
   Size:       7685
 
MluI A'CGCG_T

Cuts at:   7118    7118 
   Size:       7685
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2642    3337    6356    6540    2642 
   Size:        695    3019     184    3787

  Fragments arranged by size: 

                3787    3019     695     184
 
MscI TGG'CCA

Cuts at:   3869    5446    3869 
   Size:       1577    6108
 
MslI CAynn'nnrTG

Cuts at:    218     542    1822    4028    5674    5801     218 
   Size:        324    1280    2206    1646     127    2102

  Fragments arranged by size: 

                2206    2102    1646    1280     324     127
 
MspA1I CmG'CkG

Cuts at:    114     154    2002    2655    4364    4796    5110    5470    6483 
   Size:         40    1848     653    1709     432     314     360    1013

Cuts at:   6483    6728     114 
   Size:        245    1071

  Fragments arranged by size: 

                1848    1709    1071    1013     653     432     360     314
                 245      40
 
NaeI GCC'GGC

Cuts at:   5866    5866 
   Size:       7685
 
NarI GG'CG_CC

Cuts at:   5116    5364    5116 
   Size:        248    7437
 
NdeI CA'TA_TG

Cuts at:    438    2901     438 
   Size:       2463    5222
 
NgoMIV G'CCGG_C

Cuts at:   5864    5864 
   Size:       7685
 
NheI G'CTAG_C

Cuts at:   2808    2808 
   Size:       7685
 
NruI TCG'CGA

Cuts at:   2432    2432 
   Size:       7685
 
NsiI A_TGCA'T

Cuts at:   1419    2901    1419 
   Size:       1482    6203
 
NspI r_CATG'y

Cuts at:    565    2442    2520    4738    5238    6145     565 
   Size:       1877      78    2218     500     907    2105

  Fragments arranged by size: 

                2218    2105    1877     907     500      78
 
PciI A'CATG_T

Cuts at:    561    6141     561 
   Size:       5580    2105
 
PflMI CCAn_nnn'nTGG

Cuts at:    814     814 
   Size:       7685
 
PfoI T'CCnGG_A

Cuts at:   1955    2472    1955 
   Size:        517    7168
 
PmeI GTTT'AAAC

Cuts at:   4684    4684 
   Size:       7685
 
PmlI CAC'GTG

Cuts at:   2638    2638 
   Size:       7685
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6824    6856    6824 
   Size:         32    7653
 
PpuMI rG'GwC_Cy

Cuts at:   4398    4398 
   Size:       7685
 
PshAI GACnn'nnGTC

Cuts at:   2630    2630 
   Size:       7685
 
PsiI TTA'TAA

Cuts at:   3338    3338 
   Size:       7685
 
PstI C_TGCA'G

Cuts at:   1624    4732    5417    1624 
   Size:       3108     685    3892

  Fragments arranged by size: 

                3892    3108     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7685
 
PvuII CAG'CTG

Cuts at:   2002    2655    4364    5110    5470    2002 
   Size:        653    1709     746     360    4217

  Fragments arranged by size: 

                4217    1709     746     653     360
 
RsrII CG'GwC_CG

Cuts at:   5880    5880 
   Size:       7685
 
SacI G_AGCT'C

Cuts at:   2378    4699    2378 
   Size:       2321    5364
 
SacII CC_GC'GG

Cuts at:    155     155 
   Size:       7685
 
SalI G'TCGA_C

Cuts at:   3596    4722    3596 
   Size:       1126    6559
 
SapI GCTCTTCn'nnn_

Cuts at:   3855    5708    5918    3855 
   Size:       1853     210    5622

  Fragments arranged by size: 

                5622    1853     210
 
SbfI CC_TGCA'GG

Cuts at:   4732    4732 
   Size:       7685
 
ScaI AGT'ACT

Cuts at:   3195    7202    3195 
   Size:       4007    3678
 
SfcI C'TryA_G

Cuts at:     33    1620    1754    3980    4728    5413    6406    6597      33 
   Size:       1587     134    2226     748     685     993     191    1121

  Fragments arranged by size: 

                2226    1587    1121     993     748     685     191     134
            
 
SfoI GGC'GCC

Cuts at:   5117    5365    5117 
   Size:        248    7437
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7685
 
SmaI CCC'GGG

Cuts at:   4707    4707 
   Size:       7685
 
SmlI C'TyrA_G

Cuts at:    310    1327    2035    2561    3556    3767    3858    4840    5755 
   Size:       1017     708     526     995     211      91     982     915

Cuts at:   5755    6247    6509     310 
   Size:        492     262    1486

  Fragments arranged by size: 

                1486    1017     995     982     915     708     526     492
                 262     211      91
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7685
 
SphI G_CATG'C

Cuts at:   4738    5238    4738 
   Size:        500    7185
 
SspI AAT'ATT

Cuts at:   4188    4188 
   Size:       7685
 
StyI C'CwwG_G

Cuts at:   2423    2628    2985    3416    3644    4819    5173    2423 
   Size:        205     357     431     228    1175     354    4935

  Fragments arranged by size: 

                4935    1175     431     357     354     228     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    915    3933    4641    5268    5401    5860    6069     915 
   Size:       3018     708     627     133     459     209    2531

  Fragments arranged by size: 

                3018    2531     708     627     459     209     133
 
TatI w'GTAC_w

Cuts at:   2553    3193    3214    7200    2553 
   Size:        640      21    3986    3038

  Fragments arranged by size: 

                3986    3038     640      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    477     509    7202    7234     477 
   Size:         32    6693      32     928

  Fragments arranged by size: 

                6693     928      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   5482    5482 
   Size:       7685
 
XbaI T'CTAG_A

Cuts at:   2740    3613    3628    4716    2740 
   Size:        873      15    1088    5709

  Fragments arranged by size: 

                5709    1088     873      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1870    5180    1870 
   Size:       3310    4375
 
XmaI C'CCGG_G

Cuts at:   4705    4705 
   Size:       7685
 
XmnI GAAnn'nnTTC

Cuts at:   2889    4284    2889 
   Size:       1395    6290
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NruI     NsiI     NspI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AlfI     AloI     ApaI     AscI    AvrII
     BglI    BsiWI    BsmBI   BssHII   BstEII  BstZ17I    CspCI    EcoNI
     FseI    FspAI     NcoI     NotI     PacI     PasI   PspOMI    PspXI
     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     ZraI
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