Restriction map for
pF1KE2407
(Circular) (Six-base) MAPSORT of: pF1KE2407.seq Check: 751 from: 1 to: 7685
pF1KE2407 7685 bp
With 182 enzymes: SgfI *
May 11, 2012 13:46 ..
AccI GT'mk_AC
Cuts at: 282 3597 4723 282
Size: 3315 1126 3244
Fragments arranged by size:
3315 3244 1126
Acc65I G'GTAC_C
Cuts at: 4701 4701
Size: 7685
AclI AA'CG_TT
Cuts at: 3609 7525 3609
Size: 3916 3769
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 811 897 1586 1673 2225 2531 2843 3590
Size: 486 86 689 87 552 306 312 747
Cuts at: 3590 4125 5510 5942 6689 6957 325
Size: 535 1385 432 747 268 1053
Fragments arranged by size:
1385 1053 747 747 689 552 535 486
432 312 306 268 87 86
AflII C'TTAA_G
Cuts at: 1327 2035 1327
Size: 708 6977
AflIII A'CryG_T
Cuts at: 561 5037 6141 7118 561
Size: 4476 1104 977 1128
Fragments arranged by size:
4476 1128 1104 977
AgeI A'CCGG_T
Cuts at: 6073 6073
Size: 7685
AhdI GACnn_n'nnGTC
Cuts at: 4274 4618 4274
Size: 344 7341
AleI CACnn'nnGTG
Cuts at: 1822 1822
Size: 7685
AlwNI CAG_nnn'CTG
Cuts at: 1696 4990 6557 1696
Size: 3294 1567 2824
Fragments arranged by size:
3294 2824 1567
ApaLI G'TGCA_C
Cuts at: 2801 6455 2801
Size: 3654 4031
ApoI r'AATT_y
Cuts at: 416 2009 2097 2275 2830 4689 7038 7294 416
Size: 1593 88 178 555 1859 2349 256 807
Fragments arranged by size:
2349 1859 1593 807 555 256 178 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 7685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7685
AvaI C'yCGr_G
Cuts at: 4705 4705
Size: 7685
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 280 313 280
Size: 33 7652
BamHI G'GATC_C
Cuts at: 4710 4710
Size: 7685
BanI G'GyrC_C
Cuts at: 4701 5115 5363 5398 4701
Size: 414 248 35 6988
Fragments arranged by size:
6988 414 248 35
BanII G_rGCy'C
Cuts at: 2378 4699 5729 2378
Size: 2321 1030 4334
Fragments arranged by size:
4334 2321 1030
BbeI G_GCGC'C
Cuts at: 5119 5367 5119
Size: 248 7437
BbsI GAAGACnn'nnnn_
Cuts at: 2519 3116 3624 3674 3810 2519
Size: 597 508 50 136 6394
Fragments arranged by size:
6394 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 2852 2852
Size: 7685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7651
BciVI GTATCCnnnnn_n'
Cuts at: 2586 5583 6344 7685 2586
Size: 2997 761 1341 2586
Fragments arranged by size:
2997 2586 1341 761
BclI T'GATC_A
Cuts at: 5205 5205
Size: 7685
BfrBI ATG'CAT
Cuts at: 1417 2899 1417
Size: 1482 6203
BglII A'GATC_T
Cuts at: 203 2887 4177 5200 7237 203
Size: 2684 1290 1023 2037 651
Fragments arranged by size:
2684 2037 1290 1023 651
BlpI GC'TnA_GC
Cuts at: 4797 4797
Size: 7685
Bme1580I G_kGCm'C
Cuts at: 2805 5310 5403 6459 2805
Size: 2505 93 1056 4031
Fragments arranged by size:
4031 2505 1056 93
BmgBI CAC'GTC
Cuts at: 4620 4620
Size: 7685
BmrI ACTGGGnnnn_n'
Cuts at: 1787 4282 4622 4897 4978 5313 7457 1787
Size: 2495 340 275 81 335 2144 2015
Fragments arranged by size:
2495 2144 2015 340 335 275 81
BmtI G_CTAG'C
Cuts at: 2812 2812
Size: 7685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3385 6052 3383
Size: 2 2667 5016
Fragments arranged by size:
5016 2667 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3048 4235 2609
Size: 439 1187 6059
Fragments arranged by size:
6059 1187 439
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 7685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 2546 3541 3752 3843 4861 5740 6232 6530
Size: 2215 995 211 91 1018 879 492 298
Cuts at: 6530 331
Size: 1486
Fragments arranged by size:
2215 1486 1018 995 879 492 298 211
91
BsaI GGTCTCn'nnnn_
Cuts at: 933 3630 7389 933
Size: 2697 3759 1229
Fragments arranged by size:
3759 2697 1229
BsaAI yAC'GTr
Cuts at: 1252 2638 5038 1252
Size: 1386 2400 3899
Fragments arranged by size:
3899 2400 1386
BsaBI GATnn'nnATC
Cuts at: 6 5199 7236 6
Size: 5193 2037 455
Fragments arranged by size:
5193 2037 455
BsaHI Gr'CG_yC
Cuts at: 5116 5364 6066 7562 5116
Size: 248 702 1496 5239
Fragments arranged by size:
5239 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4874 5395 6073 6347 6494 6905 4874
Size: 521 678 274 147 411 5654
Fragments arranged by size:
5654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3171 3201 3721 3751 4190 4220 3171
Size: 30 520 30 439 30 6636
Fragments arranged by size:
6636 520 439 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1754 3615 3742 3965 4316 1754
Size: 1861 127 223 351 5123
Fragments arranged by size:
5123 1861 351 223 127
BseYI C'CCAG_C
Cuts at: 5110 5868 6445 6941 5110
Size: 758 577 496 5854
Fragments arranged by size:
5854 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1635 1660 2671 2942 1635
Size: 25 1011 271 6378
Fragments arranged by size:
6378 1011 271 25
BsiEI CG_ry'CG
Cuts at: 104 2436 5227 5273 6481 104
Size: 2332 2791 46 1208 1308
Fragments arranged by size:
2791 2332 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2378 2805 3916 4699 5477 5667 6459 2378
Size: 427 1111 783 778 190 792 3604
Fragments arranged by size:
3604 1111 792 783 778 427 190
BsmI GAATG_Cn'
Cuts at: 3459 3459
Size: 7685
Bsp1286I G_dGCh'C
Cuts at: 2378 2805 3916 4699 5310 5403 5477 5667 5729
Size: 427 1111 783 611 93 74 190 62
Cuts at: 5729 6459 2378
Size: 730 3604
Fragments arranged by size:
3604 1111 783 730 611 427 190 93
74 62
BspEI T'CCGG_A
Cuts at: 6905 6905
Size: 7685
BspHI T'CATG_A
Cuts at: 4308 6861 4308
Size: 2553 5132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 167 4735 4949 5251 5632 167
Size: 4568 214 302 381 2220
Fragments arranged by size:
4568 2220 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3288 5977 6031 7515 7680 50
Size: 3238 2689 54 1484 165 55
Fragments arranged by size:
3238 2689 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3260 4148 4430 5597 3260
Size: 888 282 1167 5348
Fragments arranged by size:
5348 1167 888 282
BsrFI r'CCGG_y
Cuts at: 4656 5683 5864 6073 4656
Size: 1027 181 209 6268
Fragments arranged by size:
6268 1027 209 181
BsrGI T'GTAC_A
Cuts at: 2553 2553
Size: 7685
BssSI C'ACGA_G
Cuts at: 767 5956 6314 767
Size: 5189 358 2138
Fragments arranged by size:
5189 2138 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5062 5062
Size: 7685
BstBI TT'CG_AA
Cuts at: 4519 6046 4519
Size: 1527 6158
BstXI CCAn_nnnn'nTGG
Cuts at: 2684 4030 4635 2684
Size: 1346 605 5734
Fragments arranged by size:
5734 1346 605
BstYI r'GATC_y
Cuts at: 1 203 1638 2565 2887 4177 4710 5177 5200
Size: 202 1435 927 322 1290 533 467 23
Cuts at: 5200 5535 5781 6793 6879 6891 7237 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1435 1290 1012 927 533 467 449 346
335 322 246 202 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2570 2570
Size: 7685
BtgI C'CryG_G
Cuts at: 152 152
Size: 7685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3500 3719 4244 5635 5816 5979 3500
Size: 219 525 1391 181 163 5206
Fragments arranged by size:
5206 1391 525 219 181 163
BtsI GCAGTG_nn'
Cuts at: 511 3240 4036 4928 511
Size: 2729 796 892 3268
Fragments arranged by size:
3268 2729 892 796
ClaI AT'CG_AT
Cuts at: 450 450
Size: 7685
DraI TTT'AAA
Cuts at: 2271 4684 2271
Size: 2413 5272
DraIII CAC_nnn'GTG
Cuts at: 1519 1519
Size: 7685
DrdI GACnn_nn'nnGTC
Cuts at: 5391 6249 5391
Size: 858 6827
EaeI y'GGCC_r
Cuts at: 3867 4290 5270 5444 5835 5862 7207 3867
Size: 423 980 174 391 27 1345 4345
Fragments arranged by size:
4345 1345 980 423 391 174 27
EagI C'GGCC_G
Cuts at: 5270 5270
Size: 7685
EarI CTCTTCn'nnn_
Cuts at: 927 1175 1568 2483 2819 3113 3728 3767 3855
Size: 248 393 915 336 294 615 39 88
Cuts at: 3855 5708 5918 927
Size: 1853 210 2694
Fragments arranged by size:
2694 1853 915 615 393 336 294 248
210 88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 5893 6203 6349 7495 199
Size: 5694 310 146 1146 389
Fragments arranged by size:
5694 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 2376 4697 2376
Size: 2321 5364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 811 897 1586 1673 2225 2531 2609 2843
Size: 486 86 689 87 552 306 78 234
Cuts at: 2843 3048 3590 4125 4235 5510 5942 6689 6957
Size: 205 542 535 110 1275 432 747 268
Cuts at: 6957 325
Size: 1053
Fragments arranged by size:
1275 1053 747 689 552 542 535 486
432 306 268 234 205 110 87 86
78
EcoO109I rG'GnC_Cy
Cuts at: 4398 4824 4398
Size: 426 7259
EcoRI G'AATT_C
Cuts at: 2009 4689 2009
Size: 2680 5005
EcoRV GAT'ATC
Cuts at: 429 1729 429
Size: 1300 6385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 647 1231 2050 3181 3325 3418 3911 5704 647
Size: 584 819 1131 144 93 493 1793 2628
Fragments arranged by size:
2628 1793 1131 819 584 493 144 93
FspI TGC'GCA
Cuts at: 5466 5466
Size: 7685
HaeII r_GCGC'y
Cuts at: 5119 5367 6389 7368 5119
Size: 248 1022 979 5436
Fragments arranged by size:
5436 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 1664 1690 1696 1722 2209 2241 3539
Size: 32 1189 26 6 26 487 32 1298
Cuts at: 3539 3571 4204 4236 4260 4292 4604 4636 5527
Size: 32 633 32 24 32 312 32 891
Cuts at: 5527 5557 5559 5589 443
Size: 30 2 30 2539
Fragments arranged by size:
2539 1298 1189 891 633 487 312 32
32 32 32 32 32 30 30 26
26 24 6 2
HincII GTy'rAC
Cuts at: 567 3598 4724 567
Size: 3031 1126 3528
Fragments arranged by size:
3528 3031 1126
HindIII A'AGCT_T
Cuts at: 781 4606 781
Size: 3825 3860
HpaI GTT'AAC
Cuts at: 567 567
Size: 7685
KasI G'GCGC_C
Cuts at: 5115 5363 5115
Size: 248 7437
KpnI G_GTAC'C
Cuts at: 4705 4705
Size: 7685
MfeI C'AATT_G
Cuts at: 659 659
Size: 7685
MluI A'CGCG_T
Cuts at: 7118 7118
Size: 7685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3337 6356 6540 2642
Size: 695 3019 184 3787
Fragments arranged by size:
3787 3019 695 184
MscI TGG'CCA
Cuts at: 3869 5446 3869
Size: 1577 6108
MslI CAynn'nnrTG
Cuts at: 218 542 1822 4028 5674 5801 218
Size: 324 1280 2206 1646 127 2102
Fragments arranged by size:
2206 2102 1646 1280 324 127
MspA1I CmG'CkG
Cuts at: 114 154 2002 2655 4364 4796 5110 5470 6483
Size: 40 1848 653 1709 432 314 360 1013
Cuts at: 6483 6728 114
Size: 245 1071
Fragments arranged by size:
1848 1709 1071 1013 653 432 360 314
245 40
NaeI GCC'GGC
Cuts at: 5866 5866
Size: 7685
NarI GG'CG_CC
Cuts at: 5116 5364 5116
Size: 248 7437
NdeI CA'TA_TG
Cuts at: 438 2901 438
Size: 2463 5222
NgoMIV G'CCGG_C
Cuts at: 5864 5864
Size: 7685
NheI G'CTAG_C
Cuts at: 2808 2808
Size: 7685
NruI TCG'CGA
Cuts at: 2432 2432
Size: 7685
NsiI A_TGCA'T
Cuts at: 1419 2901 1419
Size: 1482 6203
NspI r_CATG'y
Cuts at: 565 2442 2520 4738 5238 6145 565
Size: 1877 78 2218 500 907 2105
Fragments arranged by size:
2218 2105 1877 907 500 78
PciI A'CATG_T
Cuts at: 561 6141 561
Size: 5580 2105
PflMI CCAn_nnn'nTGG
Cuts at: 814 814
Size: 7685
PfoI T'CCnGG_A
Cuts at: 1955 2472 1955
Size: 517 7168
PmeI GTTT'AAAC
Cuts at: 4684 4684
Size: 7685
PmlI CAC'GTG
Cuts at: 2638 2638
Size: 7685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6824 6856 6824
Size: 32 7653
PpuMI rG'GwC_Cy
Cuts at: 4398 4398
Size: 7685
PshAI GACnn'nnGTC
Cuts at: 2630 2630
Size: 7685
PsiI TTA'TAA
Cuts at: 3338 3338
Size: 7685
PstI C_TGCA'G
Cuts at: 1624 4732 5417 1624
Size: 3108 685 3892
Fragments arranged by size:
3892 3108 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7685
PvuII CAG'CTG
Cuts at: 2002 2655 4364 5110 5470 2002
Size: 653 1709 746 360 4217
Fragments arranged by size:
4217 1709 746 653 360
RsrII CG'GwC_CG
Cuts at: 5880 5880
Size: 7685
SacI G_AGCT'C
Cuts at: 2378 4699 2378
Size: 2321 5364
SacII CC_GC'GG
Cuts at: 155 155
Size: 7685
SalI G'TCGA_C
Cuts at: 3596 4722 3596
Size: 1126 6559
SapI GCTCTTCn'nnn_
Cuts at: 3855 5708 5918 3855
Size: 1853 210 5622
Fragments arranged by size:
5622 1853 210
SbfI CC_TGCA'GG
Cuts at: 4732 4732
Size: 7685
ScaI AGT'ACT
Cuts at: 3195 7202 3195
Size: 4007 3678
SfcI C'TryA_G
Cuts at: 33 1620 1754 3980 4728 5413 6406 6597 33
Size: 1587 134 2226 748 685 993 191 1121
Fragments arranged by size:
2226 1587 1121 993 748 685 191 134
SfoI GGC'GCC
Cuts at: 5117 5365 5117
Size: 248 7437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7685
SmaI CCC'GGG
Cuts at: 4707 4707
Size: 7685
SmlI C'TyrA_G
Cuts at: 310 1327 2035 2561 3556 3767 3858 4840 5755
Size: 1017 708 526 995 211 91 982 915
Cuts at: 5755 6247 6509 310
Size: 492 262 1486
Fragments arranged by size:
1486 1017 995 982 915 708 526 492
262 211 91
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7685
SphI G_CATG'C
Cuts at: 4738 5238 4738
Size: 500 7185
SspI AAT'ATT
Cuts at: 4188 4188
Size: 7685
StyI C'CwwG_G
Cuts at: 2423 2628 2985 3416 3644 4819 5173 2423
Size: 205 357 431 228 1175 354 4935
Fragments arranged by size:
4935 1175 431 357 354 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 3933 4641 5268 5401 5860 6069 915
Size: 3018 708 627 133 459 209 2531
Fragments arranged by size:
3018 2531 708 627 459 209 133
TatI w'GTAC_w
Cuts at: 2553 3193 3214 7200 2553
Size: 640 21 3986 3038
Fragments arranged by size:
3986 3038 640 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 7202 7234 477
Size: 32 6693 32 928
Fragments arranged by size:
6693 928 32 32
Tth111I GACn'n_nGTC
Cuts at: 5482 5482
Size: 7685
XbaI T'CTAG_A
Cuts at: 2740 3613 3628 4716 2740
Size: 873 15 1088 5709
Fragments arranged by size:
5709 1088 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1870 5180 1870
Size: 3310 4375
XmaI C'CCGG_G
Cuts at: 4705 4705
Size: 7685
XmnI GAAnn'nnTTC
Cuts at: 2889 4284 2889
Size: 1395 6290
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BglI BsiWI BsmBI BssHII BstEII BstZ17I CspCI EcoNI
FseI FspAI NcoI NotI PacI PasI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI