(Circular) (Six-base) MAPSORT of: pF1KE2407.seq Check: 751 from: 1 to: 7685 pF1KE2407 7685 bp With 182 enzymes: SgfI * May 11, 2012 13:46 .. AccI GT'mk_AC Cuts at: 282 3597 4723 282 Size: 3315 1126 3244 Fragments arranged by size: 3315 3244 1126 Acc65I G'GTAC_C Cuts at: 4701 4701 Size: 7685 AclI AA'CG_TT Cuts at: 3609 7525 3609 Size: 3916 3769 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 325 811 897 1586 1673 2225 2531 2843 3590 Size: 486 86 689 87 552 306 312 747 Cuts at: 3590 4125 5510 5942 6689 6957 325 Size: 535 1385 432 747 268 1053 Fragments arranged by size: 1385 1053 747 747 689 552 535 486 432 312 306 268 87 86 AflII C'TTAA_G Cuts at: 1327 2035 1327 Size: 708 6977 AflIII A'CryG_T Cuts at: 561 5037 6141 7118 561 Size: 4476 1104 977 1128 Fragments arranged by size: 4476 1128 1104 977 AgeI A'CCGG_T Cuts at: 6073 6073 Size: 7685 AhdI GACnn_n'nnGTC Cuts at: 4274 4618 4274 Size: 344 7341 AleI CACnn'nnGTG Cuts at: 1822 1822 Size: 7685 AlwNI CAG_nnn'CTG Cuts at: 1696 4990 6557 1696 Size: 3294 1567 2824 Fragments arranged by size: 3294 2824 1567 ApaLI G'TGCA_C Cuts at: 2801 6455 2801 Size: 3654 4031 ApoI r'AATT_y Cuts at: 416 2009 2097 2275 2830 4689 7038 7294 416 Size: 1593 88 178 555 1859 2349 256 807 Fragments arranged by size: 2349 1859 1593 807 555 256 178 88 AseI AT'TA_AT Cuts at: 20 20 Size: 7685 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7685 AvaI C'yCGr_G Cuts at: 4705 4705 Size: 7685 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 280 313 280 Size: 33 7652 BamHI G'GATC_C Cuts at: 4710 4710 Size: 7685 BanI G'GyrC_C Cuts at: 4701 5115 5363 5398 4701 Size: 414 248 35 6988 Fragments arranged by size: 6988 414 248 35 BanII G_rGCy'C Cuts at: 2378 4699 5729 2378 Size: 2321 1030 4334 Fragments arranged by size: 4334 2321 1030 BbeI G_GCGC'C Cuts at: 5119 5367 5119 Size: 248 7437 BbsI GAAGACnn'nnnn_ Cuts at: 2519 3116 3624 3674 3810 2519 Size: 597 508 50 136 6394 Fragments arranged by size: 6394 597 508 136 50 BbvCI CC'TCA_GC Cuts at: 2852 2852 Size: 7685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 7651 BciVI GTATCCnnnnn_n' Cuts at: 2586 5583 6344 7685 2586 Size: 2997 761 1341 2586 Fragments arranged by size: 2997 2586 1341 761 BclI T'GATC_A Cuts at: 5205 5205 Size: 7685 BfrBI ATG'CAT Cuts at: 1417 2899 1417 Size: 1482 6203 BglII A'GATC_T Cuts at: 203 2887 4177 5200 7237 203 Size: 2684 1290 1023 2037 651 Fragments arranged by size: 2684 2037 1290 1023 651 BlpI GC'TnA_GC Cuts at: 4797 4797 Size: 7685 Bme1580I G_kGCm'C Cuts at: 2805 5310 5403 6459 2805 Size: 2505 93 1056 4031 Fragments arranged by size: 4031 2505 1056 93 BmgBI CAC'GTC Cuts at: 4620 4620 Size: 7685 BmrI ACTGGGnnnn_n' Cuts at: 1787 4282 4622 4897 4978 5313 7457 1787 Size: 2495 340 275 81 335 2144 2015 Fragments arranged by size: 2495 2144 2015 340 335 275 81 BmtI G_CTAG'C Cuts at: 2812 2812 Size: 7685 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3385 6052 3383 Size: 2 2667 5016 Fragments arranged by size: 5016 2667 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 3048 4235 2609 Size: 439 1187 6059 Fragments arranged by size: 6059 1187 439 Bpu10I CC'TnA_GC Cuts at: 2852 2852 Size: 7685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 331 2546 3541 3752 3843 4861 5740 6232 6530 Size: 2215 995 211 91 1018 879 492 298 Cuts at: 6530 331 Size: 1486 Fragments arranged by size: 2215 1486 1018 995 879 492 298 211 91 BsaI GGTCTCn'nnnn_ Cuts at: 933 3630 7389 933 Size: 2697 3759 1229 Fragments arranged by size: 3759 2697 1229 BsaAI yAC'GTr Cuts at: 1252 2638 5038 1252 Size: 1386 2400 3899 Fragments arranged by size: 3899 2400 1386 BsaBI GATnn'nnATC Cuts at: 6 5199 7236 6 Size: 5193 2037 455 Fragments arranged by size: 5193 2037 455 BsaHI Gr'CG_yC Cuts at: 5116 5364 6066 7562 5116 Size: 248 702 1496 5239 Fragments arranged by size: 5239 1496 702 248 BsaWI w'CCGG_w Cuts at: 4874 5395 6073 6347 6494 6905 4874 Size: 521 678 274 147 411 5654 Fragments arranged by size: 5654 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 3171 3201 3721 3751 4190 4220 3171 Size: 30 520 30 439 30 6636 Fragments arranged by size: 6636 520 439 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1754 3615 3742 3965 4316 1754 Size: 1861 127 223 351 5123 Fragments arranged by size: 5123 1861 351 223 127 BseYI C'CCAG_C Cuts at: 5110 5868 6445 6941 5110 Size: 758 577 496 5854 Fragments arranged by size: 5854 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1635 1660 2671 2942 1635 Size: 25 1011 271 6378 Fragments arranged by size: 6378 1011 271 25 BsiEI CG_ry'CG Cuts at: 104 2436 5227 5273 6481 104 Size: 2332 2791 46 1208 1308 Fragments arranged by size: 2791 2332 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2378 2805 3916 4699 5477 5667 6459 2378 Size: 427 1111 783 778 190 792 3604 Fragments arranged by size: 3604 1111 792 783 778 427 190 BsmI GAATG_Cn' Cuts at: 3459 3459 Size: 7685 Bsp1286I G_dGCh'C Cuts at: 2378 2805 3916 4699 5310 5403 5477 5667 5729 Size: 427 1111 783 611 93 74 190 62 Cuts at: 5729 6459 2378 Size: 730 3604 Fragments arranged by size: 3604 1111 783 730 611 427 190 93 74 62 BspEI T'CCGG_A Cuts at: 6905 6905 Size: 7685 BspHI T'CATG_A Cuts at: 4308 6861 4308 Size: 2553 5132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 167 4735 4949 5251 5632 167 Size: 4568 214 302 381 2220 Fragments arranged by size: 4568 2220 381 302 214 BsrBI CCG'CTC Cuts at: 50 3288 5977 6031 7515 7680 50 Size: 3238 2689 54 1484 165 55 Fragments arranged by size: 3238 2689 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3260 4148 4430 5597 3260 Size: 888 282 1167 5348 Fragments arranged by size: 5348 1167 888 282 BsrFI r'CCGG_y Cuts at: 4656 5683 5864 6073 4656 Size: 1027 181 209 6268 Fragments arranged by size: 6268 1027 209 181 BsrGI T'GTAC_A Cuts at: 2553 2553 Size: 7685 BssSI C'ACGA_G Cuts at: 767 5956 6314 767 Size: 5189 358 2138 Fragments arranged by size: 5189 2138 358 BstAPI GCAn_nnn'nTGC Cuts at: 5062 5062 Size: 7685 BstBI TT'CG_AA Cuts at: 4519 6046 4519 Size: 1527 6158 BstXI CCAn_nnnn'nTGG Cuts at: 2684 4030 4635 2684 Size: 1346 605 5734 Fragments arranged by size: 5734 1346 605 BstYI r'GATC_y Cuts at: 1 203 1638 2565 2887 4177 4710 5177 5200 Size: 202 1435 927 322 1290 533 467 23 Cuts at: 5200 5535 5781 6793 6879 6891 7237 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1435 1290 1012 927 533 467 449 346 335 322 246 202 86 23 12 Bsu36I CC'TnA_GG Cuts at: 2570 2570 Size: 7685 BtgI C'CryG_G Cuts at: 152 152 Size: 7685 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3500 3719 4244 5635 5816 5979 3500 Size: 219 525 1391 181 163 5206 Fragments arranged by size: 5206 1391 525 219 181 163 BtsI GCAGTG_nn' Cuts at: 511 3240 4036 4928 511 Size: 2729 796 892 3268 Fragments arranged by size: 3268 2729 892 796 ClaI AT'CG_AT Cuts at: 450 450 Size: 7685 DraI TTT'AAA Cuts at: 2271 4684 2271 Size: 2413 5272 DraIII CAC_nnn'GTG Cuts at: 1519 1519 Size: 7685 DrdI GACnn_nn'nnGTC Cuts at: 5391 6249 5391 Size: 858 6827 EaeI y'GGCC_r Cuts at: 3867 4290 5270 5444 5835 5862 7207 3867 Size: 423 980 174 391 27 1345 4345 Fragments arranged by size: 4345 1345 980 423 391 174 27 EagI C'GGCC_G Cuts at: 5270 5270 Size: 7685 EarI CTCTTCn'nnn_ Cuts at: 927 1175 1568 2483 2819 3113 3728 3767 3855 Size: 248 393 915 336 294 615 39 88 Cuts at: 3855 5708 5918 927 Size: 1853 210 2694 Fragments arranged by size: 2694 1853 915 615 393 336 294 248 210 88 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 199 5893 6203 6349 7495 199 Size: 5694 310 146 1146 389 Fragments arranged by size: 5694 1146 389 310 146 EcoICRI GAG'CTC Cuts at: 2376 4697 2376 Size: 2321 5364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 325 811 897 1586 1673 2225 2531 2609 2843 Size: 486 86 689 87 552 306 78 234 Cuts at: 2843 3048 3590 4125 4235 5510 5942 6689 6957 Size: 205 542 535 110 1275 432 747 268 Cuts at: 6957 325 Size: 1053 Fragments arranged by size: 1275 1053 747 689 552 542 535 486 432 306 268 234 205 110 87 86 78 EcoO109I rG'GnC_Cy Cuts at: 4398 4824 4398 Size: 426 7259 EcoRI G'AATT_C Cuts at: 2009 4689 2009 Size: 2680 5005 EcoRV GAT'ATC Cuts at: 429 1729 429 Size: 1300 6385 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 647 1231 2050 3181 3325 3418 3911 5704 647 Size: 584 819 1131 144 93 493 1793 2628 Fragments arranged by size: 2628 1793 1131 819 584 493 144 93 FspI TGC'GCA Cuts at: 5466 5466 Size: 7685 HaeII r_GCGC'y Cuts at: 5119 5367 6389 7368 5119 Size: 248 1022 979 5436 Fragments arranged by size: 5436 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 443 475 1664 1690 1696 1722 2209 2241 3539 Size: 32 1189 26 6 26 487 32 1298 Cuts at: 3539 3571 4204 4236 4260 4292 4604 4636 5527 Size: 32 633 32 24 32 312 32 891 Cuts at: 5527 5557 5559 5589 443 Size: 30 2 30 2539 Fragments arranged by size: 2539 1298 1189 891 633 487 312 32 32 32 32 32 32 30 30 26 26 24 6 2 HincII GTy'rAC Cuts at: 567 3598 4724 567 Size: 3031 1126 3528 Fragments arranged by size: 3528 3031 1126 HindIII A'AGCT_T Cuts at: 781 4606 781 Size: 3825 3860 HpaI GTT'AAC Cuts at: 567 567 Size: 7685 KasI G'GCGC_C Cuts at: 5115 5363 5115 Size: 248 7437 KpnI G_GTAC'C Cuts at: 4705 4705 Size: 7685 MfeI C'AATT_G Cuts at: 659 659 Size: 7685 MluI A'CGCG_T Cuts at: 7118 7118 Size: 7685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2642 3337 6356 6540 2642 Size: 695 3019 184 3787 Fragments arranged by size: 3787 3019 695 184 MscI TGG'CCA Cuts at: 3869 5446 3869 Size: 1577 6108 MslI CAynn'nnrTG Cuts at: 218 542 1822 4028 5674 5801 218 Size: 324 1280 2206 1646 127 2102 Fragments arranged by size: 2206 2102 1646 1280 324 127 MspA1I CmG'CkG Cuts at: 114 154 2002 2655 4364 4796 5110 5470 6483 Size: 40 1848 653 1709 432 314 360 1013 Cuts at: 6483 6728 114 Size: 245 1071 Fragments arranged by size: 1848 1709 1071 1013 653 432 360 314 245 40 NaeI GCC'GGC Cuts at: 5866 5866 Size: 7685 NarI GG'CG_CC Cuts at: 5116 5364 5116 Size: 248 7437 NdeI CA'TA_TG Cuts at: 438 2901 438 Size: 2463 5222 NgoMIV G'CCGG_C Cuts at: 5864 5864 Size: 7685 NheI G'CTAG_C Cuts at: 2808 2808 Size: 7685 NruI TCG'CGA Cuts at: 2432 2432 Size: 7685 NsiI A_TGCA'T Cuts at: 1419 2901 1419 Size: 1482 6203 NspI r_CATG'y Cuts at: 565 2442 2520 4738 5238 6145 565 Size: 1877 78 2218 500 907 2105 Fragments arranged by size: 2218 2105 1877 907 500 78 PciI A'CATG_T Cuts at: 561 6141 561 Size: 5580 2105 PflMI CCAn_nnn'nTGG Cuts at: 814 814 Size: 7685 PfoI T'CCnGG_A Cuts at: 1955 2472 1955 Size: 517 7168 PmeI GTTT'AAAC Cuts at: 4684 4684 Size: 7685 PmlI CAC'GTG Cuts at: 2638 2638 Size: 7685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6824 6856 6824 Size: 32 7653 PpuMI rG'GwC_Cy Cuts at: 4398 4398 Size: 7685 PshAI GACnn'nnGTC Cuts at: 2630 2630 Size: 7685 PsiI TTA'TAA Cuts at: 3338 3338 Size: 7685 PstI C_TGCA'G Cuts at: 1624 4732 5417 1624 Size: 3108 685 3892 Fragments arranged by size: 3892 3108 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 7685 PvuII CAG'CTG Cuts at: 2002 2655 4364 5110 5470 2002 Size: 653 1709 746 360 4217 Fragments arranged by size: 4217 1709 746 653 360 RsrII CG'GwC_CG Cuts at: 5880 5880 Size: 7685 SacI G_AGCT'C Cuts at: 2378 4699 2378 Size: 2321 5364 SacII CC_GC'GG Cuts at: 155 155 Size: 7685 SalI G'TCGA_C Cuts at: 3596 4722 3596 Size: 1126 6559 SapI GCTCTTCn'nnn_ Cuts at: 3855 5708 5918 3855 Size: 1853 210 5622 Fragments arranged by size: 5622 1853 210 SbfI CC_TGCA'GG Cuts at: 4732 4732 Size: 7685 ScaI AGT'ACT Cuts at: 3195 7202 3195 Size: 4007 3678 SfcI C'TryA_G Cuts at: 33 1620 1754 3980 4728 5413 6406 6597 33 Size: 1587 134 2226 748 685 993 191 1121 Fragments arranged by size: 2226 1587 1121 993 748 685 191 134 SfoI GGC'GCC Cuts at: 5117 5365 5117 Size: 248 7437 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7685 SmaI CCC'GGG Cuts at: 4707 4707 Size: 7685 SmlI C'TyrA_G Cuts at: 310 1327 2035 2561 3556 3767 3858 4840 5755 Size: 1017 708 526 995 211 91 982 915 Cuts at: 5755 6247 6509 310 Size: 492 262 1486 Fragments arranged by size: 1486 1017 995 982 915 708 526 492 262 211 91 SpeI A'CTAG_T Cuts at: 67 67 Size: 7685 SphI G_CATG'C Cuts at: 4738 5238 4738 Size: 500 7185 SspI AAT'ATT Cuts at: 4188 4188 Size: 7685 StyI C'CwwG_G Cuts at: 2423 2628 2985 3416 3644 4819 5173 2423 Size: 205 357 431 228 1175 354 4935 Fragments arranged by size: 4935 1175 431 357 354 228 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 915 3933 4641 5268 5401 5860 6069 915 Size: 3018 708 627 133 459 209 2531 Fragments arranged by size: 3018 2531 708 627 459 209 133 TatI w'GTAC_w Cuts at: 2553 3193 3214 7200 2553 Size: 640 21 3986 3038 Fragments arranged by size: 3986 3038 640 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 477 509 7202 7234 477 Size: 32 6693 32 928 Fragments arranged by size: 6693 928 32 32 Tth111I GACn'n_nGTC Cuts at: 5482 5482 Size: 7685 XbaI T'CTAG_A Cuts at: 2740 3613 3628 4716 2740 Size: 873 15 1088 5709 Fragments arranged by size: 5709 1088 873 15 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1870 5180 1870 Size: 3310 4375 XmaI C'CCGG_G Cuts at: 4705 4705 Size: 7685 XmnI GAAnn'nnTTC Cuts at: 2889 4284 2889 Size: 1395 6290 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NruI NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AlfI AloI ApaI AscI AvrII BglI BsiWI BsmBI BssHII BstEII BstZ17I CspCI EcoNI FseI FspAI NcoI NotI PacI PasI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI