Restriction map for
pF1KE2398
(Circular) (Six-base) MAPSORT of: pF1KE2398.seq Check: 6002 from: 1 to: 4127
pF1KE2398 4127 bp
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AatII G_ACGT'C
Cuts at: 878 878
Size: 4127
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AfeI AGC'GCT
Cuts at: 616 616
Size: 4127
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 641 2515 641
Size: 1874 2253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 672 672
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaI G_GGCC'C
Cuts at: 846 846
Size: 4127
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 1131 3480 3736 1131
Size: 2349 256 1522
Fragments arranged by size:
2349 1522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 1147 1147
Size: 4127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 348 381 348
Size: 33 4094
BamHI G'GATC_C
Cuts at: 275 1152 275
Size: 877 3250
BanI G'GyrC_C
Cuts at: 1143 1557 1805 1840 1143
Size: 414 248 35 3430
Fragments arranged by size:
3430 414 248 35
BanII G_rGCy'C
Cuts at: 378 846 1116 1141 2171 378
Size: 468 270 25 1030 2334
Fragments arranged by size:
2334 1030 468 270 25
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbsI GAAGACnn'nnnn_
Cuts at: 322 322
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 437 1647 437
Size: 1210 2917
BglI GCCn_nnn'nGGC
Cuts at: 176 995 176
Size: 819 3308
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 846 1752 1845 2901 846
Size: 906 93 1056 2072
Fragments arranged by size:
2072 1056 906 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 1339 1420 1755 3899 348
Size: 991 81 335 2144 576
Fragments arranged by size:
2144 991 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 429 301
Size: 128 3999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1303 2182 2674 2972 364
Size: 939 879 492 298 1519
Fragments arranged by size:
1519 939 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3831 3831
Size: 4127
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 194 425 875 1558 1806 2508 4004 194
Size: 231 450 683 248 702 1496 317
Fragments arranged by size:
1496 702 683 450 317 248 231
BsaWI w'CCGG_w
Cuts at: 641 662 701 794 965 1316 1837 2515 2789
Size: 21 39 93 171 351 521 678 274
Cuts at: 2789 2936 3347 641
Size: 147 411 1421
Fragments arranged by size:
1421 678 521 411 351 274 171 147
93 39 21
BseYI C'CCAG_C
Cuts at: 553 1552 2310 2887 3383 553
Size: 999 758 577 496 1297
Fragments arranged by size:
1297 999 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 467
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 857 939 1669 1715 2923 104
Size: 753 82 730 46 1208 1308
Fragments arranged by size:
1308 1208 753 730 82 46
BsiHKAI G_wGCw'C
Cuts at: 287 1116 1141 1919 2109 2901 287
Size: 829 25 778 190 792 1513
Fragments arranged by size:
1513 829 792 778 190 25
BsmI GAATG_Cn'
Cuts at: 329 329
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 287 378 846 1116 1141 1752 1845 1919 2109
Size: 91 468 270 25 611 93 74 190
Cuts at: 2109 2171 2901 287
Size: 62 730 1513
Fragments arranged by size:
1513 730 611 468 270 190 93 91
74 62 25
BspEI T'CCGG_A
Cuts at: 662 965 3347 662
Size: 303 2382 1442
Fragments arranged by size:
2382 1442 303
BspHI T'CATG_A
Cuts at: 434 3303 434
Size: 2869 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 771 1177 1391 1693 2074 230
Size: 541 406 214 302 381 2283
Fragments arranged by size:
2283 541 406 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 641 938 994 2125 2306 2515 641
Size: 297 56 1131 181 209 2253
Fragments arranged by size:
2253 1131 297 209 181 56
BsrGI T'GTAC_A
Cuts at: 626 626
Size: 4127
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 618 1504 618
Size: 886 3241
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstYI r'GATC_y
Cuts at: 1 275 342 1152 1619 1642 1977 2223 3235
Size: 274 67 810 467 23 335 246 1012
Cuts at: 3235 3321 3333 3679 1
Size: 86 12 346 449
Fragments arranged by size:
1012 810 467 449 346 335 274 246
86 67 23 12
BtgI C'CryG_G
Cuts at: 107 851 107
Size: 744 3383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 280 2077 2258 2421 280
Size: 1797 181 163 1986
Fragments arranged by size:
1986 1797 181 163
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 503 1126 503
Size: 623 3504
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 269 563 836 854 923 936 953 992 1712
Size: 294 273 18 69 13 17 39 720
Cuts at: 1712 1886 2277 2304 3649 269
Size: 174 391 27 1345 747
Fragments arranged by size:
1345 747 720 391 294 273 174 69
39 27 18 17 13
EagI C'GGCC_G
Cuts at: 854 936 1712 854
Size: 82 776 3269
Fragments arranged by size:
3269 776 82
EarI CTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1114 1139 1114
Size: 25 4102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 429 1952 2384 3131 3399 301
Size: 128 1523 432 747 268 1029
Fragments arranged by size:
1523 1029 747 432 268 128
EcoO109I rG'GnC_Cy
Cuts at: 413 692 842 843 1266 413
Size: 279 150 1 423 3274
Fragments arranged by size:
3274 423 279 150 1
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 2146 633
Size: 1513 2614
FspI TGC'GCA
Cuts at: 118 1908 118
Size: 1790 2337
HaeII r_GCGC'y
Cuts at: 236 618 1098 1561 1809 2831 3810 236
Size: 382 480 463 248 1022 979 553
Fragments arranged by size:
1022 979 553 480 463 382 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 327 353 359 1969 1999 2001 2031 321
Size: 6 26 6 1610 30 2 30 2417
Fragments arranged by size:
2417 1610 30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 429 786 1166 429
Size: 357 380 3390
Fragments arranged by size:
3390 380 357
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 2798 2982 185
Size: 2613 184 1330
Fragments arranged by size:
2613 1330 184
MscI TGG'CCA
Cuts at: 565 925 955 1888 565
Size: 360 30 933 2804
Fragments arranged by size:
2804 933 360 30
MslI CAynn'nnrTG
Cuts at: 250 439 2116 2243 250
Size: 189 1677 127 2134
Fragments arranged by size:
2134 1677 189 127
MspA1I CmG'CkG
Cuts at: 122 656 720 853 935 1101 1238 1552 1912
Size: 534 64 133 82 166 137 314 360
Cuts at: 1912 2925 3170 122
Size: 1013 245 1079
Fragments arranged by size:
1079 1013 534 360 314 245 166 137
133 82 64
NaeI GCC'GGC
Cuts at: 940 996 2308 940
Size: 56 1312 2759
Fragments arranged by size:
2759 1312 56
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NcoI C'CATG_G
Cuts at: 107 107
Size: 4127
NgoMIV G'CCGG_C
Cuts at: 938 994 2306 938
Size: 56 1312 2759
Fragments arranged by size:
2759 1312 56
NotI GC'GGCC_GC
Cuts at: 854 854
Size: 4127
NspI r_CATG'y
Cuts at: 1180 1680 2587 1180
Size: 500 907 2720
Fragments arranged by size:
2720 907 500
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PflMI CCAn_nnn'nTGG
Cuts at: 144 1055 144
Size: 911 3216
PfoI T'CCnGG_A
Cuts at: 278 278
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PspOMI G'GGCC_C
Cuts at: 842 842
Size: 4127
PstI C_TGCA'G
Cuts at: 776 1174 1859 776
Size: 398 685 3044
Fragments arranged by size:
3044 685 398
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 656 720 1101 1552 1912 656
Size: 64 381 451 360 2871
Fragments arranged by size:
2871 451 381 360 64
RsrII CG'GwC_CG
Cuts at: 395 2322 395
Size: 1927 2200
SacI G_AGCT'C
Cuts at: 1116 1141 1116
Size: 25 4102
SacII CC_GC'GG
Cuts at: 854 854
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 776 1174 776
Size: 398 3729
ScaI AGT'ACT
Cuts at: 1081 3644 1081
Size: 2563 1564
SfcI C'TryA_G
Cuts at: 33 772 1170 1855 2848 3039 33
Size: 739 398 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 398 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 379 1282 2197 2689 2951 379
Size: 903 915 492 262 1555
Fragments arranged by size:
1555 915 903 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
StyI C'CwwG_G
Cuts at: 107 522 1261 1615 107
Size: 415 739 354 2619
Fragments arranged by size:
2619 739 415 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 279 412 433 694 1710 1843 2302 2511
Size: 17 133 21 261 1016 133 459 209
Cuts at: 2511 262
Size: 1878
Fragments arranged by size:
1878 1016 459 261 209 133 133 21
17
TatI w'GTAC_w
Cuts at: 356 626 1079 3642 356
Size: 270 453 2563 841
Fragments arranged by size:
2563 841 453 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4095
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 867 1098 1622 867
Size: 231 524 3372
Fragments arranged by size:
3372 524 231
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
ZraI GAC'GTC
Cuts at: 876 876
Size: 4127
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI AscI AvrII BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI