Restriction map for
pF1KE2395
(Circular) (Six-base) MAPSORT of: pF1KE2395.seq Check: 1384 from: 1 to: 6500
pF1KE2395 6500 bp
With 182 enzymes: SgfI *
January 26, 2012 13:40 ..
AccI GT'mk_AC
Cuts at: 976 3538 976
Size: 2562 3938
Acc65I G'GTAC_C
Cuts at: 2014 3516 2014
Size: 1502 4998
AclI AA'CG_TT
Cuts at: 6340 6340
Size: 6500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1190 2030 3305 3362 4325 4757 5504 5772
Size: 816 840 1275 57 963 432 747 268
Cuts at: 5772 374
Size: 1102
Fragments arranged by size:
1275 1102 963 840 816 747 432 268
57
AflIII A'CryG_T
Cuts at: 613 3360 3852 4956 5933 613
Size: 2747 492 1104 977 1180
Fragments arranged by size:
2747 1180 1104 977 492
AgeI A'CCGG_T
Cuts at: 4888 4888
Size: 6500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1514 3044 1514
Size: 1530 4970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2752 2784 2752
Size: 32 6468
AlwNI CAG_nnn'CTG
Cuts at: 3805 5372 3805
Size: 1567 4933
ApaLI G'TGCA_C
Cuts at: 5270 5270
Size: 6500
ApoI r'AATT_y
Cuts at: 599 1943 2440 2487 2781 2889 3054 3420 3504
Size: 1344 497 47 294 108 165 366 84
Cuts at: 3504 5853 6109 599
Size: 2349 256 990
Fragments arranged by size:
2349 1344 990 497 366 294 256 165
108 84 47
AseI AT'TA_AT
Cuts at: 20 260 857 2524 20
Size: 240 597 1667 3996
Fragments arranged by size:
3996 1667 597 240
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6500
AvaI C'yCGr_G
Cuts at: 3520 3520
Size: 6500
BamHI G'GATC_C
Cuts at: 3525 3525
Size: 6500
BanI G'GyrC_C
Cuts at: 2014 3516 3930 4178 4213 2014
Size: 1502 414 248 35 4301
Fragments arranged by size:
4301 1502 414 248 35
BanII G_rGCy'C
Cuts at: 3514 4544 3514
Size: 1030 5470
BbeI G_GCGC'C
Cuts at: 3934 4182 3934
Size: 248 6252
BbsI GAAGACnn'nnnn_
Cuts at: 236 1196 1282 236
Size: 960 86 5454
Fragments arranged by size:
5454 960 86
BbvCI CC'TCA_GC
Cuts at: 2702 2702
Size: 6500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6466
BciVI GTATCCnnnnn_n'
Cuts at: 2155 4398 5159 6500 2155
Size: 2243 761 1341 2155
Fragments arranged by size:
2243 2155 1341 761
BclI T'GATC_A
Cuts at: 2124 4020 2124
Size: 1896 4604
BglII A'GATC_T
Cuts at: 1578 4015 6052 1578
Size: 2437 2037 2026
Fragments arranged by size:
2437 2037 2026
BlpI GC'TnA_GC
Cuts at: 3612 3612
Size: 6500
Bme1580I G_kGCm'C
Cuts at: 1761 4125 4218 5274 1761
Size: 2364 93 1056 2987
Fragments arranged by size:
2987 2364 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1019 2142 3712 3793 4128 6272 1019
Size: 1123 1570 81 335 2144 1247
Fragments arranged by size:
2144 1570 1247 1123 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4867 4867
Size: 6500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 2452
Size: 6500
Bpu10I CC'TnA_GC
Cuts at: 2702 3017 2702
Size: 315 6185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4555 5047 5345 3676
Size: 879 492 298 4831
Fragments arranged by size:
4831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 827 6204 827
Size: 5377 1123
BsaAI yAC'GTr
Cuts at: 2028 2078 3853 2028
Size: 50 1775 4675
Fragments arranged by size:
4675 1775 50
BsaBI GATnn'nnATC
Cuts at: 6 245 1892 4014 6051 6
Size: 239 1647 2122 2037 455
Fragments arranged by size:
2122 2037 1647 455 239
BsaHI Gr'CG_yC
Cuts at: 3931 4179 4881 6377 3931
Size: 248 702 1496 4054
Fragments arranged by size:
4054 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3689 4210 4888 5162 5309 5720 3689
Size: 521 678 274 147 411 4469
Fragments arranged by size:
4469 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 807 837 2067 2097 807
Size: 30 1230 30 5210
Fragments arranged by size:
5210 1230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1315 1318 2299 3125 1315
Size: 3 981 826 4690
Fragments arranged by size:
4690 981 826 3
BseYI C'CCAG_C
Cuts at: 1923 2159 3120 3925 4683 5260 5756 1923
Size: 236 961 805 758 577 496 2667
Fragments arranged by size:
2667 961 805 758 577 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2831 2933 1633
Size: 1198 102 5200
Fragments arranged by size:
5200 1198 102
BsiEI CG_ry'CG
Cuts at: 104 4042 4088 5296 104
Size: 3938 46 1208 1308
Fragments arranged by size:
3938 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2108 2365 3514 4292 4482 5274 2108
Size: 257 1149 778 190 792 3334
Fragments arranged by size:
3334 1149 792 778 257 190
BsmI GAATG_Cn'
Cuts at: 1328 2057 2174 2637 1328
Size: 729 117 463 5191
Fragments arranged by size:
5191 729 463 117
Bsp1286I G_dGCh'C
Cuts at: 1761 2108 2365 3514 4125 4218 4292 4482 4544
Size: 347 257 1149 611 93 74 190 62
Cuts at: 4544 5274 1761
Size: 730 2987
Fragments arranged by size:
2987 1149 730 611 347 257 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5720 5720
Size: 6500
BspHI T'CATG_A
Cuts at: 1196 5676 1196
Size: 4480 2020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 3550 3764 4066 4447 838
Size: 2712 214 302 381 2891
Fragments arranged by size:
2891 2712 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4792 4846 6330 6495 50
Size: 4742 54 1484 165 55
Fragments arranged by size:
4742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4412 4412
Size: 6500
BsrFI r'CCGG_y
Cuts at: 4498 4679 4888 4498
Size: 181 209 6110
Fragments arranged by size:
6110 209 181
BssSI C'ACGA_G
Cuts at: 4771 5129 4771
Size: 358 6142
BstAPI GCAn_nnn'nTGC
Cuts at: 3877 3877
Size: 6500
BstBI TT'CG_AA
Cuts at: 2049 4861 2049
Size: 2812 3688
BstEII G'GTnAC_C
Cuts at: 1954 1954
Size: 6500
BstXI CCAn_nnnn'nTGG
Cuts at: 1509 1509
Size: 6500
BstYI r'GATC_y
Cuts at: 1 442 580 1142 1578 3525 3992 4015 4350
Size: 441 138 562 436 1947 467 23 335
Cuts at: 4350 4596 5608 5694 5706 6052 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1947 1012 562 467 449 441 436 346
335 246 138 86 23 12
BstZ17I GTA'TAC
Cuts at: 977 977
Size: 6500
Bsu36I CC'TnA_GG
Cuts at: 545 545
Size: 6500
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4450 4631 4794 4450
Size: 181 163 6156
Fragments arranged by size:
6156 181 163
BtsI GCAGTG_nn'
Cuts at: 311 631 1641 2106 2945 3743 311
Size: 320 1010 465 839 798 3068
Fragments arranged by size:
3068 1010 839 798 465 320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 386 421 704 739 2570 2605 386
Size: 35 283 35 1831 35 4281
Fragments arranged by size:
4281 1831 283 35 35 35
DraI TTT'AAA
Cuts at: 3499 3499
Size: 6500
DrdI GACnn_nn'nnGTC
Cuts at: 1226 4206 5064 1226
Size: 2980 858 2662
Fragments arranged by size:
2980 2662 858
EaeI y'GGCC_r
Cuts at: 628 4085 4259 4650 4677 6022 628
Size: 3457 174 391 27 1345 1106
Fragments arranged by size:
3457 1345 1106 391 174 27
EagI C'GGCC_G
Cuts at: 4085 4085
Size: 6500
EarI CTCTTCn'nnn_
Cuts at: 289 744 1967 2351 2588 4523 4733 289
Size: 455 1223 384 237 1935 210 2056
Fragments arranged by size:
2056 1935 1223 455 384 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4708 5018 5164 6310 4708
Size: 310 146 1146 4898
Fragments arranged by size:
4898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3512 3512
Size: 6500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1190 2030 2452 3305 3362 4325 4757 5504
Size: 816 840 422 853 57 963 432 747
Cuts at: 5504 5772 374
Size: 268 1102
Fragments arranged by size:
1102 963 853 840 816 747 432 422
268 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 2183
Size: 6500
EcoO109I rG'GnC_Cy
Cuts at: 3639 3639
Size: 6500
EcoRI G'AATT_C
Cuts at: 2440 2889 3504 2440
Size: 449 615 5436
Fragments arranged by size:
5436 615 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 773 3112 4519 570
Size: 203 2339 1407 2551
Fragments arranged by size:
2551 2339 1407 203
FspI TGC'GCA
Cuts at: 4281 4281
Size: 6500
HaeII r_GCGC'y
Cuts at: 3934 4182 5204 6183 3934
Size: 248 1022 979 4251
Fragments arranged by size:
4251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1237 1269 4342 4372 4374 4404 1237
Size: 32 3073 30 2 30 3333
Fragments arranged by size:
3333 3073 32 30 30 2
HincII GTy'rAC
Cuts at: 3281 3539 3281
Size: 258 6242
HindIII A'AGCT_T
Cuts at: 1636 2242 1636
Size: 606 5894
KasI G'GCGC_C
Cuts at: 3930 4178 3930
Size: 248 6252
KpnI G_GTAC'C
Cuts at: 2018 3520 2018
Size: 1502 4998
MluI A'CGCG_T
Cuts at: 5933 5933
Size: 6500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 246 5171 5355 246
Size: 4925 184 1391
Fragments arranged by size:
4925 1391 184
MscI TGG'CCA
Cuts at: 630 4261 630
Size: 3631 2869
MslI CAynn'nnrTG
Cuts at: 282 1507 2884 4489 4616 282
Size: 1225 1377 1605 127 2166
Fragments arranged by size:
2166 1605 1377 1225 127
MspA1I CmG'CkG
Cuts at: 2008 3611 3925 4285 5298 5543 2008
Size: 1603 314 360 1013 245 2965
Fragments arranged by size:
2965 1603 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4681 4681
Size: 6500
NarI GG'CG_CC
Cuts at: 3931 4179 3931
Size: 248 6252
NdeI CA'TA_TG
Cuts at: 1720 1720
Size: 6500
NgoMIV G'CCGG_C
Cuts at: 4679 4679
Size: 6500
NspI r_CATG'y
Cuts at: 617 1610 3553 4053 4960 617
Size: 993 1943 500 907 2157
Fragments arranged by size:
2157 1943 993 907 500
PciI A'CATG_T
Cuts at: 613 4956 613
Size: 4343 2157
PflMI CCAn_nnn'nTGG
Cuts at: 700 700
Size: 6500
PmeI GTTT'AAAC
Cuts at: 3499 3499
Size: 6500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 5639
Size: 32 6468
PsiI TTA'TAA
Cuts at: 1076 1168 2989 1076
Size: 92 1821 4587
Fragments arranged by size:
4587 1821 92
PstI C_TGCA'G
Cuts at: 318 679 849 3033 3547 4232 318
Size: 361 170 2184 514 685 2586
Fragments arranged by size:
2586 2184 685 514 361 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6500
PvuII CAG'CTG
Cuts at: 2008 3925 4285 2008
Size: 1917 360 4223
Fragments arranged by size:
4223 1917 360
RsrII CG'GwC_CG
Cuts at: 4695 4695
Size: 6500
SacI G_AGCT'C
Cuts at: 3514 3514
Size: 6500
SalI G'TCGA_C
Cuts at: 3537 3537
Size: 6500
SapI GCTCTTCn'nnn_
Cuts at: 4523 4733 4523
Size: 210 6290
SbfI CC_TGCA'GG
Cuts at: 3547 3547
Size: 6500
ScaI AGT'ACT
Cuts at: 6017 6017
Size: 6500
SexAI A'CCwGG_T
Cuts at: 817 817
Size: 6500
SfcI C'TryA_G
Cuts at: 33 314 675 845 2555 2654 3029 3543 4228
Size: 281 361 170 1710 99 375 514 685
Cuts at: 4228 5221 5412 33
Size: 993 191 1121
Fragments arranged by size:
1710 1121 993 685 514 375 361 281
191 170 99
SfoI GGC'GCC
Cuts at: 3932 4180 3932
Size: 248 6252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6500
SmaI CCC'GGG
Cuts at: 3522 3522
Size: 6500
SmlI C'TyrA_G
Cuts at: 3655 4570 5062 5324 3655
Size: 915 492 262 4831
Fragments arranged by size:
4831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1753 67
Size: 1686 4814
SphI G_CATG'C
Cuts at: 3553 4053 3553
Size: 500 6000
SspI AAT'ATT
Cuts at: 838 2910 3204 838
Size: 2072 294 4134
Fragments arranged by size:
4134 2072 294
StyI C'CwwG_G
Cuts at: 1902 2671 2829 3634 3988 1902
Size: 769 158 805 354 4414
Fragments arranged by size:
4414 805 769 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1717 2124 4083 4216 4675 4884 1717
Size: 407 1959 133 459 209 3333
Fragments arranged by size:
3333 1959 459 407 209 133
TatI w'GTAC_w
Cuts at: 2776 6015 2776
Size: 3239 3261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1811 1843 6017 6049 1811
Size: 32 4174 32 2262
Fragments arranged by size:
4174 2262 32 32
Tth111I GACn'n_nGTC
Cuts at: 4297 4297
Size: 6500
XbaI T'CTAG_A
Cuts at: 1005 3531 1005
Size: 2526 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 897 3995 897
Size: 3098 3402
XmaI C'CCGG_G
Cuts at: 3520 3520
Size: 6500
XmnI GAAnn'nnTTC
Cuts at: 359 359
Size: 6500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BtgI ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI