Restriction map for
pF1KE2381
(Circular) (Six-base) MAPSORT of: pF1KE2381.seq Check: 8296 from: 1 to: 7583
pF1KE2381 7583 bp
With 182 enzymes: SgfI *
October 25, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4061 4061
Size: 7583
AatII G_ACGT'C
Cuts at: 3255 3255
Size: 7583
AccI GT'mk_AC
Cuts at: 3051 4621 3051
Size: 1570 6013
Acc65I G'GTAC_C
Cuts at: 2807 4599 2807
Size: 1792 5791
AclI AA'CG_TT
Cuts at: 1984 2841 7423 1984
Size: 857 4582 2144
Fragments arranged by size:
4582 2144 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1517 2392 2786 3662 4052 4390 5408 5840
Size: 820 875 394 876 390 338 1018 432
Cuts at: 5840 6587 6855 697
Size: 747 268 1425
Fragments arranged by size:
1425 1018 876 875 820 747 432 394
390 338 268
AflIII A'CryG_T
Cuts at: 2092 4935 6039 7016 2092
Size: 2843 1104 977 2659
Fragments arranged by size:
2843 2659 1104 977
AgeI A'CCGG_T
Cuts at: 5971 5971
Size: 7583
AleI CACnn'nnGTG
Cuts at: 2459 2459
Size: 7583
AlwNI CAG_nnn'CTG
Cuts at: 1333 1486 3555 4215 4888 6455 1333
Size: 153 2069 660 673 1567 2461
Fragments arranged by size:
2461 2069 1567 673 660 153
ApaI G_GGCC'C
Cuts at: 3830 4492 3830
Size: 662 6921
ApaLI G'TGCA_C
Cuts at: 2593 3132 6353 2593
Size: 539 3221 3823
Fragments arranged by size:
3823 3221 539
ApoI r'AATT_y
Cuts at: 1527 1905 2216 2667 3061 4587 6936 7192 1527
Size: 378 311 451 394 1526 2349 256 1918
Fragments arranged by size:
2349 1918 1526 451 394 378 311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7583
AvaI C'yCGr_G
Cuts at: 464 819 873 915 4603 464
Size: 355 54 42 3688 3444
Fragments arranged by size:
3688 3444 355 54 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1928 1961 1928
Size: 33 7550
BamHI G'GATC_C
Cuts at: 4608 4608
Size: 7583
BanI G'GyrC_C
Cuts at: 2527 2807 4599 5013 5261 5296 2527
Size: 280 1792 414 248 35 4814
Fragments arranged by size:
4814 1792 414 280 248 35
BanII G_rGCy'C
Cuts at: 775 988 2132 3830 3914 4492 4597 5627 775
Size: 213 1144 1698 84 578 105 1030 2731
Fragments arranged by size:
2731 1698 1144 1030 578 213 105 84
BbeI G_GCGC'C
Cuts at: 5017 5265 5017
Size: 248 7335
BbsI GAAGACnn'nnnn_
Cuts at: 661 1942 2250 4493 661
Size: 1281 308 2243 3751
Fragments arranged by size:
3751 2243 1281 308
BbvCI CC'TCA_GC
Cuts at: 2189 3368 4376 2189
Size: 1179 1008 5396
Fragments arranged by size:
5396 1179 1008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 421 455 90
Size: 34 297 34 7218
Fragments arranged by size:
7218 297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5481 6242 7583 5481
Size: 761 1341 5481
Fragments arranged by size:
5481 1341 761
BclI T'GATC_A
Cuts at: 2948 5103 2948
Size: 2155 5428
BglI GCCn_nnn'nGGC
Cuts at: 3825 3825
Size: 7583
BglII A'GATC_T
Cuts at: 1006 2142 5098 7135 1006
Size: 1136 2956 2037 1454
Fragments arranged by size:
2956 2037 1454 1136
BlpI GC'TnA_GC
Cuts at: 2936 4695 2936
Size: 1759 5824
Bme1580I G_kGCm'C
Cuts at: 301 689 2597 3136 3585 3830 4282 4492 5208
Size: 388 1908 539 449 245 452 210 716
Cuts at: 5208 5301 6357 301
Size: 93 1056 1527
Fragments arranged by size:
1908 1527 1056 716 539 452 449 388
245 210 93
BmgBI CAC'GTC
Cuts at: 2095 2095
Size: 7583
BmrI ACTGGGnnnn_n'
Cuts at: 1073 1145 2271 3658 4460 4491 4795 4876 5211
Size: 72 1126 1387 802 31 304 81 335
Cuts at: 5211 7355 1073
Size: 2144 1301
Fragments arranged by size:
2144 1387 1301 1126 802 335 304 81
72 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1318 3518 5950 1318
Size: 2200 2432 2951
Fragments arranged by size:
2951 2432 2200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 2039 2108 2147 2801 3182 3808 501
Size: 1538 69 39 654 381 626 4276
Fragments arranged by size:
4276 1538 654 626 381 69 39
Bpu10I CC'TnA_GC
Cuts at: 1117 1418 2189 3368 3493 3908 4335 4376 4464
Size: 301 771 1179 125 415 427 41 88
Cuts at: 4464 1117
Size: 4236
Fragments arranged by size:
4236 1179 771 427 415 301 125 88
41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1925 3768 4095 4759 5638 6130 6428 1925
Size: 1843 327 664 879 492 298 3080
Fragments arranged by size:
3080 1843 879 664 492 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 1133 1672 2202 3613 4071 4402 7287 1133
Size: 539 530 1411 458 331 2885 1429
Fragments arranged by size:
2885 1429 1411 539 530 458 331
BsaAI yAC'GTr
Cuts at: 4936 4936
Size: 7583
BsaBI GATnn'nnATC
Cuts at: 6 5097 7134 6
Size: 5091 2037 455
Fragments arranged by size:
5091 2037 455
BsaHI Gr'CG_yC
Cuts at: 3048 3252 5014 5262 5964 7460 3048
Size: 204 1762 248 702 1496 3171
Fragments arranged by size:
3171 1762 1496 702 248 204
BsaWI w'CCGG_w
Cuts at: 542 695 4772 5293 5971 6245 6392 6803 542
Size: 153 4077 521 678 274 147 411 1322
Fragments arranged by size:
4077 1322 678 521 411 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 664 690 694 720 1722 1752 1974
Size: 30 294 26 4 26 1002 30 222
Cuts at: 1974 2004 2348 2378 3514 3544 340
Size: 30 344 30 1136 30 4379
Fragments arranged by size:
4379 1136 1002 344 294 222 30 30
30 30 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 167 589 1651 3185 3649 3866 3871 4580 167
Size: 422 1062 1534 464 217 5 709 3170
Fragments arranged by size:
3170 1534 1062 709 464 422 217 5
BseYI C'CCAG_C
Cuts at: 3357 3400 3602 3990 4025 4434 5008 5766 6343
Size: 43 202 388 35 409 574 758 577
Cuts at: 6343 6839 3357
Size: 496 4101
Fragments arranged by size:
4101 758 577 574 496 409 388 202
43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 4312 415
Size: 3897 3686
BsiEI CG_ry'CG
Cuts at: 104 426 1036 5125 5171 6379 104
Size: 322 610 4089 46 1208 1308
Fragments arranged by size:
4089 1308 1208 610 322 46
BsiHKAI G_wGCw'C
Cuts at: 2132 2597 3136 4067 4597 5375 5565 6357 2132
Size: 465 539 931 530 778 190 792 3358
Fragments arranged by size:
3358 931 792 778 539 530 465 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1023 1023
Size: 7583
Bsp1286I G_dGCh'C
Cuts at: 301 689 775 988 2132 2597 3136 3585 3830
Size: 388 86 213 1144 465 539 449 245
Cuts at: 3830 3914 4067 4282 4492 4597 5208 5301 5375
Size: 84 153 215 210 105 611 93 74
Cuts at: 5375 5565 5627 6357 301
Size: 190 62 730 1527
Fragments arranged by size:
1527 1144 730 611 539 465 449 388
245 215 213 210 190 153 105 93
86 84 74 62
BspEI T'CCGG_A
Cuts at: 6803 6803
Size: 7583
BspHI T'CATG_A
Cuts at: 2624 6759 2624
Size: 4135 3448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 3479 4061 4633 4847 5149 5530 591
Size: 2888 582 572 214 302 381 2644
Fragments arranged by size:
2888 2644 582 572 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5875 5929 7413 7578 50
Size: 5825 54 1484 165 55
Fragments arranged by size:
5825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5495 5495
Size: 7583
BsrFI r'CCGG_y
Cuts at: 5581 5762 5971 5581
Size: 181 209 7193
Fragments arranged by size:
7193 209 181
BsrGI T'GTAC_A
Cuts at: 1920 1920
Size: 7583
BssSI C'ACGA_G
Cuts at: 5854 6212 5854
Size: 358 7225
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 3658 4960 1333
Size: 2325 1302 3956
Fragments arranged by size:
3956 2325 1302
BstBI TT'CG_AA
Cuts at: 568 5944 568
Size: 5376 2207
BstEII G'GTnAC_C
Cuts at: 273 977 273
Size: 704 6879
BstXI CCAn_nnnn'nTGG
Cuts at: 264 2984 264
Size: 2720 4863
BstYI r'GATC_y
Cuts at: 1 763 1006 1228 2142 3117 4608 5075 5098
Size: 762 243 222 914 975 1491 467 23
Cuts at: 5098 5433 5679 6691 6777 6789 7135 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1491 1012 975 914 762 467 449 346
335 246 243 222 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3321 3321
Size: 7583
BtgI C'CryG_G
Cuts at: 3623 3623
Size: 7583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5533 5714 5877 5533
Size: 181 163 7239
Fragments arranged by size:
7239 181 163
BtsI GCAGTG_nn'
Cuts at: 3464 4826 3464
Size: 1362 6221
ClaI AT'CG_AT
Cuts at: 532 532
Size: 7583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3795 3830 3795
Size: 35 7548
DraI TTT'AAA
Cuts at: 4582 4582
Size: 7583
DraIII CAC_nnn'GTG
Cuts at: 4350 4350
Size: 7583
DrdI GACnn_nn'nnGTC
Cuts at: 5289 6147 5289
Size: 858 6725
EaeI y'GGCC_r
Cuts at: 834 2322 4047 5168 5342 5733 5760 7105 834
Size: 1488 1725 1121 174 391 27 1345 1312
Fragments arranged by size:
1725 1488 1345 1312 1121 391 174 27
EagI C'GGCC_G
Cuts at: 5168 5168
Size: 7583
EarI CTCTTCn'nnn_
Cuts at: 1219 1697 1790 2057 2762 3275 3892 4551 5606
Size: 478 93 267 705 513 617 659 1055
Cuts at: 5606 5816 1219
Size: 210 2986
Fragments arranged by size:
2986 1055 705 659 617 513 478 267
210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5791 6101 6247 7393 5791
Size: 310 146 1146 5981
Fragments arranged by size:
5981 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2130 4595 2130
Size: 2465 5118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 697 1517 2039 2108 2147 2392 2786 2801
Size: 196 820 522 69 39 245 394 15
Cuts at: 2801 3182 3662 3808 4052 4390 5408 5840 6587
Size: 381 480 146 244 338 1018 432 747
Cuts at: 6587 6855 501
Size: 268 1229
Fragments arranged by size:
1229 1018 820 747 522 480 432 394
381 338 268 245 244 196 146 69
39 15
EcoNI CCTnn'n_nnAGG
Cuts at: 918 993 1023 4142 918
Size: 75 30 3119 4359
Fragments arranged by size:
4359 3119 75 30
EcoO109I rG'GnC_Cy
Cuts at: 3826 3827 3902 4229 4311 4329 4488 4528 4722
Size: 1 75 327 82 18 159 40 194
Cuts at: 4722 3826
Size: 6687
Fragments arranged by size:
6687 327 194 159 82 75 40 18
1
EcoRI G'AATT_C
Cuts at: 3061 4587 3061
Size: 1526 6057
EcoRV GAT'ATC
Cuts at: 333 548 333
Size: 215 7368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1076 2068 3979 5602 1076
Size: 992 1911 1623 3057
Fragments arranged by size:
3057 1911 1623 992
FspI TGC'GCA
Cuts at: 5364 5364
Size: 7583
HaeII r_GCGC'y
Cuts at: 3974 5017 5265 6287 7266 3974
Size: 1043 248 1022 979 4291
Fragments arranged by size:
4291 1043 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 340 354 372 532 564 664 690 696
Size: 18 14 18 160 32 100 26 6
Cuts at: 696 722 802 834 1125 1157 1549 1581 1632
Size: 26 80 32 291 32 392 32 51
Cuts at: 1632 1664 2079 2111 3102 3134 5425 5455 5457
Size: 32 415 32 991 32 2291 30 2
Cuts at: 5457 5487 322
Size: 30 2418
Fragments arranged by size:
2418 2291 991 415 392 291 160 100
80 51 32 32 32 32 32 32
32 30 30 26 26 18 18 14
6 2
HincII GTy'rAC
Cuts at: 2469 4622 2469
Size: 2153 5430
HindIII A'AGCT_T
Cuts at: 3701 3701
Size: 7583
HpaI GTT'AAC
Cuts at: 2469 2469
Size: 7583
KasI G'GCGC_C
Cuts at: 5013 5261 5013
Size: 248 7335
KpnI G_GTAC'C
Cuts at: 2811 4603 2811
Size: 1792 5791
MfeI C'AATT_G
Cuts at: 1700 1700
Size: 7583
MluI A'CGCG_T
Cuts at: 7016 7016
Size: 7583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 1117 1535 3557 6254 6438 371
Size: 746 418 2022 2697 184 1516
Fragments arranged by size:
2697 2022 1516 746 418 184
MscI TGG'CCA
Cuts at: 836 2324 4049 5344 836
Size: 1488 1725 1295 3075
Fragments arranged by size:
3075 1725 1488 1295
MslI CAynn'nnrTG
Cuts at: 2459 5572 5699 2459
Size: 3113 127 4343
Fragments arranged by size:
4343 3113 127
MspA1I CmG'CkG
Cuts at: 1067 1330 1957 2980 3139 3438 3516 3555 3718
Size: 263 627 1023 159 299 78 39 163
Cuts at: 3718 3756 4694 5008 5368 6381 6626 1067
Size: 38 938 314 360 1013 245 2024
Fragments arranged by size:
2024 1023 1013 938 627 360 314 299
263 245 163 159 78 39 38
NaeI GCC'GGC
Cuts at: 5764 5764
Size: 7583
NarI GG'CG_CC
Cuts at: 5014 5262 5014
Size: 248 7335
NcoI C'CATG_G
Cuts at: 3623 3623
Size: 7583
NgoMIV G'CCGG_C
Cuts at: 5762 5762
Size: 7583
NspI r_CATG'y
Cuts at: 3108 3726 3872 4405 4636 5136 6043 3108
Size: 618 146 533 231 500 907 4648
Fragments arranged by size:
4648 907 618 533 500 231 146
PasI CC'CwG_GG
Cuts at: 294 294
Size: 7583
PciI A'CATG_T
Cuts at: 6039 6039
Size: 7583
PflMI CCAn_nnn'nTGG
Cuts at: 4215 4215
Size: 7583
PfoI T'CCnGG_A
Cuts at: 766 903 766
Size: 137 7446
PmeI GTTT'AAAC
Cuts at: 4582 4582
Size: 7583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1218 1250 6722 6754 1218
Size: 32 5472 32 2047
Fragments arranged by size:
5472 2047 32 32
PpuMI rG'GwC_Cy
Cuts at: 4229 4311 4329 4229
Size: 82 18 7483
Fragments arranged by size:
7483 82 18
PshAI GACnn'nnGTC
Cuts at: 401 851 1035 401
Size: 450 184 6949
Fragments arranged by size:
6949 450 184
PspOMI G'GGCC_C
Cuts at: 3826 4488 3826
Size: 662 6921
PspXI vC'TCGA_Gb
Cuts at: 873 873
Size: 7583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 7551
PstI C_TGCA'G
Cuts at: 1344 3084 3479 4630 5315 1344
Size: 1740 395 1151 685 3612
Fragments arranged by size:
3612 1740 1151 685 395
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7583
PvuII CAG'CTG
Cuts at: 1330 1957 2980 3139 3438 3516 3555 3718 3756
Size: 627 1023 159 299 78 39 163 38
Cuts at: 3756 5008 5368 1330
Size: 1252 360 3545
Fragments arranged by size:
3545 1252 1023 627 360 299 163 159
78 39 38
RsrII CG'GwC_CG
Cuts at: 5778 5778
Size: 7583
SacI G_AGCT'C
Cuts at: 2132 4597 2132
Size: 2465 5118
SalI G'TCGA_C
Cuts at: 4620 4620
Size: 7583
SanDI GG'GwC_CC
Cuts at: 4329 4329
Size: 7583
SapI GCTCTTCn'nnn_
Cuts at: 1790 3275 5606 5816 1790
Size: 1485 2331 210 3557
Fragments arranged by size:
3557 2331 1485 210
SbfI CC_TGCA'GG
Cuts at: 4630 4630
Size: 7583
ScaI AGT'ACT
Cuts at: 1848 3333 4090 7100 1848
Size: 1485 757 3010 2331
Fragments arranged by size:
3010 2331 1485 757
SfcI C'TryA_G
Cuts at: 33 990 1020 1340 3080 3151 3475 4626 5311
Size: 957 30 320 1740 71 324 1151 685
Cuts at: 5311 6304 6495 33
Size: 993 191 1121
Fragments arranged by size:
1740 1151 1121 993 957 685 324 320
191 71 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 3825 3825
Size: 7583
SfoI GGC'GCC
Cuts at: 5015 5263 5015
Size: 248 7335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7583
SmaI CCC'GGG
Cuts at: 466 4605 466
Size: 4139 3444
SmlI C'TyrA_G
Cuts at: 873 915 1940 3747 4074 4738 5653 6145 6407
Size: 42 1025 1807 327 664 915 492 262
Cuts at: 6407 873
Size: 2049
Fragments arranged by size:
2049 1807 1025 915 664 492 327 262
42
SpeI A'CTAG_T
Cuts at: 67 2361 67
Size: 2294 5289
SphI G_CATG'C
Cuts at: 4636 5136 4636
Size: 500 7083
StuI AGG'CCT
Cuts at: 3416 4072 4475 3416
Size: 656 403 6524
Fragments arranged by size:
6524 656 403
StyI C'CwwG_G
Cuts at: 1367 2339 3623 4202 4254 4717 5071 1367
Size: 972 1284 579 52 463 354 3879
Fragments arranged by size:
3879 1284 972 579 463 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 398 505 741 918 1092 5166 5299 5758 5967
Size: 107 236 177 174 4074 133 459 209
Cuts at: 5967 398
Size: 2014
Fragments arranged by size:
4074 2014 459 236 209 177 174 133
107
TatI w'GTAC_w
Cuts at: 1846 1920 3331 4088 7098 1846
Size: 74 1411 757 3010 2331
Fragments arranged by size:
3010 2331 1411 757 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 2097 2129 2259 2291 2348 2380 2674
Size: 32 1620 32 130 32 57 32 294
Cuts at: 2674 2706 7100 7132 445
Size: 32 4394 32 896
Fragments arranged by size:
4394 1620 896 294 130 57 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4103 5380 4103
Size: 1277 6306
XbaI T'CTAG_A
Cuts at: 4614 4614
Size: 7583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2928 5078 2928
Size: 2150 5433
XhoI C'TCGA_G
Cuts at: 873 915 873
Size: 42 7541
XmaI C'CCGG_G
Cuts at: 464 4603 464
Size: 4139 3444
XmnI GAAnn'nnTTC
Cuts at: 2051 2051
Size: 7583
ZraI GAC'GTC
Cuts at: 3253 3253
Size: 7583
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BfrBI
BmtI BsiWI BsmI BssHII BstZ17I FseI FspAI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI SacII
SexAI SgrAI SnaBI SrfI SspI SwaI