Restriction map for
pF1KE2365
(Circular) (Six-base) MAPSORT of: pF1KE2365.seq Check: 6504 from: 1 to: 3842
pF1KE2365 3842 bp
With 182 enzymes: SgfI *
August 4, 2011 11:52 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1667 2099 2846 3114 1667
Size: 432 747 268 2395
Fragments arranged by size:
2395 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AleI CACnn'nnGTG
Cuts at: 421 421
Size: 3842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 200 200
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 810 1147 2714 810
Size: 337 1567 1938
Fragments arranged by size:
1938 1567 337
ApaI G_GGCC'C
Cuts at: 604 604
Size: 3842
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 472 862 472
Size: 390 3452
BamHI G'GATC_C
Cuts at: 824 867 824
Size: 43 3799
BanI G'GyrC_C
Cuts at: 285 858 1272 1520 1555 285
Size: 573 414 248 35 2572
Fragments arranged by size:
2572 573 414 248 35
BanII G_rGCy'C
Cuts at: 472 604 708 856 1886 472
Size: 132 104 148 1030 2428
Fragments arranged by size:
2428 1030 148 132 104
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbsI GAAGACnn'nnnn_
Cuts at: 440 440
Size: 3842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 444 478 90
Size: 34 320 34 3454
Fragments arranged by size:
3454 320 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 772 1740 2501 3842 772
Size: 968 761 1341 772
Fragments arranged by size:
1341 968 772 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 604 1467 1560 2616 604
Size: 863 93 1056 1830
Fragments arranged by size:
1830 1056 863 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 3842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 716 1018 1897 2389 2687 541
Size: 175 302 879 492 298 1696
Fragments arranged by size:
1696 879 492 302 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 583 3546 583
Size: 2963 879
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 644 1273 1521 2223 3719 644
Size: 629 248 702 1496 767
Fragments arranged by size:
1496 767 702 629 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 714 118
Size: 596 3246
BseYI C'CCAG_C
Cuts at: 533 1267 2025 2602 3098 533
Size: 734 758 577 496 1277
Fragments arranged by size:
1277 758 734 577 496
BsiEI CG_ry'CG
Cuts at: 104 115 1384 1430 2638 104
Size: 11 1269 46 1208 1308
Fragments arranged by size:
1308 1269 1208 46 11
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 472 604 708 856 1467 1560 1634 1824 1886
Size: 132 104 148 611 93 74 190 62
Cuts at: 1886 2616 472
Size: 730 1698
Fragments arranged by size:
1698 730 611 190 148 132 104 93
74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 719 3018 719
Size: 2299 1543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 398 572 692 1840 2021 2230 398
Size: 174 120 1148 181 209 2010
Fragments arranged by size:
2010 1148 209 181 174 120
BssSI C'ACGA_G
Cuts at: 307 2113 2471 307
Size: 1806 358 1678
Fragments arranged by size:
1806 1678 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstEII G'GTnAC_C
Cuts at: 575 575
Size: 3842
BstXI CCAn_nnnn'nTGG
Cuts at: 213 213
Size: 3842
BstYI r'GATC_y
Cuts at: 1 824 867 1334 1357 1692 1938 2950 3036
Size: 823 43 467 23 335 246 1012 86
Cuts at: 3036 3048 3394 1
Size: 12 346 449
Fragments arranged by size:
1012 823 467 449 346 335 246 86
43 23 12
BtgI C'CryG_G
Cuts at: 115 156 348 820 115
Size: 41 192 472 3137
Fragments arranged by size:
3137 472 192 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 300 527 1085 300
Size: 227 558 3057
Fragments arranged by size:
3057 558 227
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 112 134 674 1427 1601 1992 2019 3364 112
Size: 22 540 753 174 391 27 1345 590
Fragments arranged by size:
1345 753 590 540 391 174 27 22
EagI C'GGCC_G
Cuts at: 112 1427 112
Size: 1315 2527
EarI CTCTTCn'nnn_
Cuts at: 350 556 1865 2075 350
Size: 206 1309 210 2117
Fragments arranged by size:
2117 1309 210 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 711 2050 2360 2506 3652 711
Size: 1339 310 146 1146 901
Fragments arranged by size:
1339 1146 901 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1667 2099 2846 3114 157
Size: 1510 432 747 268 885
Fragments arranged by size:
1510 885 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 600 981 600
Size: 381 3461
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 259 1623 259
Size: 1364 2478
FspAI rTGC'GCAy
Cuts at: 259 259
Size: 3842
HaeII r_GCGC'y
Cuts at: 282 1276 1524 2546 3525 282
Size: 994 248 1022 979 599
Fragments arranged by size:
1022 994 979 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 662 694 1684 1714 1716 1746 662
Size: 32 990 30 2 30 2758
Fragments arranged by size:
2758 990 32 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 676 1603 676
Size: 927 2915
MslI CAynn'nnrTG
Cuts at: 196 421 1831 1958 196
Size: 225 1410 127 2080
Fragments arranged by size:
2080 1410 225 127
MspA1I CmG'CkG
Cuts at: 117 144 501 953 1267 1627 2640 2885 117
Size: 27 357 452 314 360 1013 245 1074
Fragments arranged by size:
1074 1013 452 360 357 314 245 27
NaeI GCC'GGC
Cuts at: 694 2023 694
Size: 1329 2513
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 156 820 156
Size: 664 3178
NgoMIV G'CCGG_C
Cuts at: 692 2021 692
Size: 1329 2513
NotI GC'GGCC_GC
Cuts at: 112 112
Size: 3842
NspI r_CATG'y
Cuts at: 258 895 1395 2302 258
Size: 637 500 907 1798
Fragments arranged by size:
1798 907 637 500
PasI CC'CwG_GG
Cuts at: 666 666
Size: 3842
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PspOMI G'GGCC_C
Cuts at: 600 600
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 144 501 1267 1627 144
Size: 357 766 360 2359
Fragments arranged by size:
2359 766 360 357
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SacII CC_GC'GG
Cuts at: 118 118
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 783 885 1570 2563 2754 33
Size: 750 102 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 191 102
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 556 731 997 1912 2404 2666 556
Size: 175 266 915 492 262 1732
Fragments arranged by size:
1732 915 492 266 262 175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 156 624 820 976 1330 156
Size: 468 196 156 354 2668
Fragments arranged by size:
2668 468 354 196 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 772 1337 772
Size: 565 3277
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI