Restriction map for
pF1KE2364
(Circular) (Six-base) MAPSORT of: pF1KE2364.seq Check: 8444 from: 1 to: 3818
pF1KE2364 3818 bp
With 182 enzymes: SgfI *
August 4, 2011 11:52 ..
AatII G_ACGT'C
Cuts at: 262 262
Size: 3818
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 834 834
Size: 3818
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 465 1643 2075 2822 3090 250
Size: 215 1178 432 747 268 978
Fragments arranged by size:
1178 978 747 432 268 215
AflIII A'CryG_T
Cuts at: 744 1170 2274 3251 744
Size: 426 1104 977 1311
Fragments arranged by size:
1311 1104 977 426
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 243 576 1123 2690 243
Size: 333 547 1567 1371
Fragments arranged by size:
1567 1371 547 333
ApaLI G'TGCA_C
Cuts at: 622 2588 622
Size: 1966 1852
ApoI r'AATT_y
Cuts at: 822 3171 3427 822
Size: 2349 256 1213
Fragments arranged by size:
2349 1213 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 375 838 375
Size: 463 3355
BamHI G'GATC_C
Cuts at: 843 843
Size: 3818
BanI G'GyrC_C
Cuts at: 292 552 666 834 1248 1496 1531 292
Size: 260 114 168 414 248 35 2579
Fragments arranged by size:
2579 414 260 248 168 114 35
BanII G_rGCy'C
Cuts at: 832 1862 832
Size: 1030 2788
BbeI G_GCGC'C
Cuts at: 296 556 670 1252 1500 296
Size: 260 114 582 248 2614
Fragments arranged by size:
2614 582 260 248 114
BbsI GAAGACnn'nnnn_
Cuts at: 492 747 492
Size: 255 3563
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglII A'GATC_T
Cuts at: 1333 3370 1333
Size: 2037 1781
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 626 1443 1536 2592 626
Size: 817 93 1056 1852
Fragments arranged by size:
1852 1056 817 93
BmgBI CAC'GTC
Cuts at: 745 745
Size: 3818
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
Bpu10I CC'TnA_GC
Cuts at: 412 460 412
Size: 48 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3522 3522
Size: 3818
BsaAI yAC'GTr
Cuts at: 1171 1171
Size: 3818
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 259 293 526 553 667 1249 1497 2199 3695
Size: 34 233 27 114 582 248 702 1496
Cuts at: 3695 259
Size: 382
Fragments arranged by size:
1496 702 582 382 248 233 114 34
27
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 3788
BseYI C'CCAG_C
Cuts at: 365 790 1243 2001 2578 3074 365
Size: 425 453 758 577 496 1109
Fragments arranged by size:
1109 758 577 496 453 425
BsiEI CG_ry'CG
Cuts at: 104 350 1360 1406 2614 104
Size: 246 1010 46 1208 1308
Fragments arranged by size:
1308 1208 1010 246 46
BsiHKAI G_wGCw'C
Cuts at: 626 832 1610 1800 2592 626
Size: 206 778 190 792 1852
Fragments arranged by size:
1852 792 778 206 190
Bsp1286I G_dGCh'C
Cuts at: 626 832 1443 1536 1610 1800 1862 2592 626
Size: 206 611 93 74 190 62 730 1852
Fragments arranged by size:
1852 730 611 206 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1082 1384 1765 868
Size: 214 302 381 2921
Fragments arranged by size:
2921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2110 2164 3648 3813 50
Size: 2060 54 1484 165 55
Fragments arranged by size:
2060 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1730
Size: 3818
BsrFI r'CCGG_y
Cuts at: 495 549 599 1816 1997 2206 495
Size: 54 50 1217 181 209 2107
Fragments arranged by size:
2107 1217 209 181 54 50
BssSI C'ACGA_G
Cuts at: 270 627 2089 2447 270
Size: 357 1462 358 1641
Fragments arranged by size:
1641 1462 358 357
BstAPI GCAn_nnn'nTGC
Cuts at: 1195 1195
Size: 3818
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstYI r'GATC_y
Cuts at: 1 843 1310 1333 1668 1914 2926 3012 3024
Size: 842 467 23 335 246 1012 86 12
Cuts at: 3024 3370 1
Size: 346 449
Fragments arranged by size:
1012 842 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 546 1768 1949 2112 546
Size: 1222 181 163 2252
Fragments arranged by size:
2252 1222 181 163
BtsI GCAGTG_nn'
Cuts at: 626 1061 626
Size: 435 3383
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 400 1524 2382 400
Size: 1124 858 1836
Fragments arranged by size:
1836 1124 858
EaeI y'GGCC_r
Cuts at: 347 601 674 1403 1577 1968 1995 3340 347
Size: 254 73 729 174 391 27 1345 825
Fragments arranged by size:
1345 825 729 391 254 174 73 27
EagI C'GGCC_G
Cuts at: 347 1403 347
Size: 1056 2762
EarI CTCTTCn'nnn_
Cuts at: 283 1841 2051 283
Size: 1558 210 2050
Fragments arranged by size:
2050 1558 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 412 2026 2336 2482 3628 412
Size: 1614 310 146 1146 602
Fragments arranged by size:
1614 1146 602 310 146
EcoICRI GAG'CTC
Cuts at: 830 830
Size: 3818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 465 1643 2075 2822 3090 250
Size: 215 1178 432 747 268 978
Fragments arranged by size:
1178 978 747 432 268 215
EcoO109I rG'GnC_Cy
Cuts at: 690 957 690
Size: 267 3551
EcoRI G'AATT_C
Cuts at: 822 822
Size: 3818
EcoRV GAT'ATC
Cuts at: 654 654
Size: 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 296 535 556 670 1252 1500 2522 3501 296
Size: 239 21 114 582 248 1022 979 613
Fragments arranged by size:
1022 979 613 582 248 239 114 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1660 1690 1692 1722 1660
Size: 30 2 30 3756
Fragments arranged by size:
3756 30 30 2
HincII GTy'rAC
Cuts at: 857 857
Size: 3818
KasI G'GCGC_C
Cuts at: 292 552 666 1248 1496 292
Size: 260 114 582 248 2614
Fragments arranged by size:
2614 582 260 248 114
KpnI G_GTAC'C
Cuts at: 838 838
Size: 3818
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 2489 2673 275
Size: 2214 184 1420
Fragments arranged by size:
2214 1420 184
MscI TGG'CCA
Cuts at: 1579 1579
Size: 3818
MslI CAynn'nnrTG
Cuts at: 1807 1934 1807
Size: 127 3691
MspA1I CmG'CkG
Cuts at: 141 192 369 607 929 1243 1603 2616 2861
Size: 51 177 238 322 314 360 1013 245
Cuts at: 2861 141
Size: 1098
Fragments arranged by size:
1098 1013 360 322 314 245 238 177
51
NaeI GCC'GGC
Cuts at: 601 1999 601
Size: 1398 2420
NarI GG'CG_CC
Cuts at: 293 553 667 1249 1497 293
Size: 260 114 582 248 2614
Fragments arranged by size:
2614 582 260 248 114
NcoI C'CATG_G
Cuts at: 107 107
Size: 3818
NgoMIV G'CCGG_C
Cuts at: 599 1997 599
Size: 1398 2420
NspI r_CATG'y
Cuts at: 871 1371 2278 871
Size: 500 907 2411
Fragments arranged by size:
2411 907 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PpuMI rG'GwC_Cy
Cuts at: 690 690
Size: 3818
PstI C_TGCA'G
Cuts at: 865 1550 865
Size: 685 3133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 832 832
Size: 3818
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 283 1841 2051 283
Size: 1558 210 2050
Fragments arranged by size:
2050 1558 210
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 861 1546 2539 2730 33
Size: 828 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 191
SfoI GGC'GCC
Cuts at: 294 554 668 1250 1498 294
Size: 260 114 582 248 2614
Fragments arranged by size:
2614 582 260 248 114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SgrAI Cr'CCGG_yG
Cuts at: 549 549
Size: 3818
SmaI CCC'GGG
Cuts at: 840 840
Size: 3818
SmlI C'TyrA_G
Cuts at: 973 1888 2380 2642 973
Size: 915 492 262 2149
Fragments arranged by size:
2149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
StyI C'CwwG_G
Cuts at: 107 341 671 952 1306 107
Size: 234 330 281 354 2619
Fragments arranged by size:
2619 354 330 281 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 379 1401 1534 1993 2202 379
Size: 1022 133 459 209 1995
Fragments arranged by size:
1995 1022 459 209 133
TatI w'GTAC_w
Cuts at: 476 3333 476
Size: 2857 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 217 249 3335 3367 217
Size: 32 3086 32 668
Fragments arranged by size:
3086 668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XmaI C'CCGG_G
Cuts at: 838 838
Size: 3818
ZraI GAC'GTC
Cuts at: 260 260
Size: 3818
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI