Restriction map for
pF1KE2356
(Circular) (Six-base) MAPSORT of: pF1KE2356.seq Check: 6532 from: 1 to: 11288
pF1KE2356 11288 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4530 5854 4530
Size: 1324 9964
AatII G_ACGT'C
Cuts at: 4364 4364
Size: 11288
AccI GT'mk_AC
Cuts at: 1043 8326 1043
Size: 7283 4005
Acc65I G'GTAC_C
Cuts at: 5206 8304 5206
Size: 3098 8190
AclI AA'CG_TT
Cuts at: 11128 11128
Size: 11288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1079 2233 5363 6028 6106 9113 9545 10292
Size: 571 1154 3130 665 78 3007 432 747
Cuts at: 10292 10560 508
Size: 268 1236
Fragments arranged by size:
3130 3007 1236 1154 747 665 571 432
268 78
AflIII A'CryG_T
Cuts at: 404 5999 8640 9744 10721 404
Size: 5595 2641 1104 977 971
Fragments arranged by size:
5595 2641 1104 977 971
AgeI A'CCGG_T
Cuts at: 7666 9676 7666
Size: 2010 9278
AleI CACnn'nnGTG
Cuts at: 1906 2749 4015 4564 5998 1906
Size: 843 1266 549 1434 7196
Fragments arranged by size:
7196 1434 1266 843 549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 997 5911 997
Size: 4914 6374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1687 1719 1687
Size: 32 11256
AlwNI CAG_nnn'CTG
Cuts at: 600 2551 3880 4633 6566 7118 8593 10160 600
Size: 1951 1329 753 1933 552 1475 1567 1728
Fragments arranged by size:
1951 1933 1728 1567 1475 1329 753 552
ApaI G_GGCC'C
Cuts at: 1940 2542 2731 3212 3412 4485 4558 4643 5074
Size: 602 189 481 200 1073 73 85 431
Cuts at: 5074 5803 6962 8035 1940
Size: 729 1159 1073 5193
Fragments arranged by size:
5193 1159 1073 1073 729 602 481 431
200 189 85 73
ApaLI G'TGCA_C
Cuts at: 2026 7438 8122 10058 2026
Size: 5412 684 1936 3256
Fragments arranged by size:
5412 3256 1936 684
ApoI r'AATT_y
Cuts at: 3817 8292 10641 10897 3817
Size: 4475 2349 256 4208
Fragments arranged by size:
4475 4208 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11288
AvaI C'yCGr_G
Cuts at: 328 418 427 761 2398 3315 3570 3993 4484
Size: 90 9 334 1637 917 255 423 491
Cuts at: 4484 4849 5233 5523 5919 6060 7124 7415 7433
Size: 365 384 290 396 141 1064 291 18
Cuts at: 7433 7449 7651 7710 8027 8308 328
Size: 16 202 59 317 281 3308
Fragments arranged by size:
3308 1637 1064 917 491 423 396 384
365 334 317 291 290 281 255 202
141 90 59 18 16 9
BamHI G'GATC_C
Cuts at: 611 6802 8313 611
Size: 6191 1511 3586
Fragments arranged by size:
6191 3586 1511
BanI G'GyrC_C
Cuts at: 624 682 798 901 1474 1615 1728 1900 2278
Size: 58 116 103 573 141 113 172 378
Cuts at: 2278 2988 3246 3340 3736 3772 4036 4243 4530
Size: 710 258 94 396 36 264 207 287
Cuts at: 4530 4603 4677 5059 5206 5386 5424 5566 5842
Size: 73 74 382 147 180 38 142 276
Cuts at: 5842 5986 6424 6537 7045 7302 7411 7558 8098
Size: 144 438 113 508 257 109 147 540
Cuts at: 8098 8304 8718 8966 9001 624
Size: 206 414 248 35 2911
Fragments arranged by size:
2911 710 573 540 508 438 414 396
382 378 287 276 264 258 257 248
207 206 180 172 147 147 144 142
141 116 113 113 109 103 94 74
73 58 38 36 35
BanII G_rGCy'C
Cuts at: 311 1634 1807 1940 2365 2459 2542 2731 2971
Size: 1323 173 133 425 94 83 189 240
Cuts at: 2971 3212 3412 4123 4402 4485 4558 4643 5074
Size: 241 200 711 279 83 73 85 431
Cuts at: 5074 5177 5339 5501 5653 5803 5929 6077 6422
Size: 103 162 162 152 150 126 148 345
Cuts at: 6422 6962 7370 7652 7975 8035 8302 9332 311
Size: 540 408 282 323 60 267 1030 2267
Fragments arranged by size:
2267 1323 1030 711 540 431 425 408
345 323 282 279 267 241 240 200
189 173 162 162 152 150 148 133
126 103 94 85 83 83 73 60
BbeI G_GCGC'C
Cuts at: 686 4534 5390 8722 8970 686
Size: 3848 856 3332 248 3004
Fragments arranged by size:
3848 3332 3004 856 248
BbsI GAAGACnn'nnnn_
Cuts at: 2440 5536 2440
Size: 3096 8192
BbvCI CC'TCA_GC
Cuts at: 298 3004 3515 5452 298
Size: 2706 511 1937 6134
Fragments arranged by size:
6134 2706 1937 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 453 487 3561 3595 5007 5041 453
Size: 34 3074 34 1412 34 6700
Fragments arranged by size:
6700 3074 1412 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9186 9947 11288 9186
Size: 761 1341 9186
Fragments arranged by size:
9186 1341 761
BclI T'GATC_A
Cuts at: 5102 8808 5102
Size: 3706 7582
BglI GCCn_nnn'nGGC
Cuts at: 766 1865 2803 6102 6881 766
Size: 1099 938 3299 779 5173
Fragments arranged by size:
5173 3299 1099 938 779
BglII A'GATC_T
Cuts at: 4838 8803 10840 4838
Size: 3965 2037 5286
Fragments arranged by size:
5286 3965 2037
BlpI GC'TnA_GC
Cuts at: 337 8400 337
Size: 8063 3225
Bme1580I G_kGCm'C
Cuts at: 629 803 906 1133 1479 1663 1940 2030 2303
Size: 174 103 227 346 184 277 90 273
Cuts at: 2303 2357 2542 2731 3212 3412 3815 4115 4246
Size: 54 185 189 481 200 403 300 131
Cuts at: 4246 4485 4558 4643 5074 5803 5989 6057 6947
Size: 239 73 85 431 729 186 68 890
Cuts at: 6947 6962 7001 7050 7416 7442 7563 8035 8126
Size: 15 39 49 366 26 121 472 91
Cuts at: 8126 8913 9006 10062 629
Size: 787 93 1056 1855
Fragments arranged by size:
1855 1056 890 787 729 481 472 431
403 366 346 300 277 273 239 227
200 189 186 185 184 174 131 121
103 93 91 90 85 73 68 54
49 39 26 15
BmgBI CAC'GTC
Cuts at: 2031 2502 6000 2031
Size: 471 3498 7319
Fragments arranged by size:
7319 3498 471
BmrI ACTGGGnnnn_n'
Cuts at: 732 867 1927 5015 5412 8500 8581 8916 11060
Size: 135 1060 3088 397 3088 81 335 2144
Cuts at: 11060 732
Size: 960
Fragments arranged by size:
3088 3088 2144 1060 960 397 335 135
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1956 4293 5355 6861 9655 1956
Size: 2337 1062 1506 2794 3589
Fragments arranged by size:
3589 2794 2337 1506 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1411 2158 3172 3622 4207 4370 5789 5897
Size: 993 747 1014 450 585 163 1419 108
Cuts at: 5897 7099 7339 7959 418
Size: 1202 240 620 3747
Fragments arranged by size:
3747 1419 1202 1014 993 747 620 585
450 240 163 108
Bpu10I CC'TnA_GC
Cuts at: 298 1079 3004 3515 4452 5452 5713 298
Size: 781 1925 511 937 1000 261 5873
Fragments arranged by size:
5873 1925 1000 937 781 511 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1618 2428 3307 6565 7354 7947 8464 9343
Size: 1377 810 879 3258 789 593 517 879
Cuts at: 9343 9835 10133 241
Size: 492 298 1396
Fragments arranged by size:
3258 1396 1377 879 879 810 789 593
517 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 715 2201 3325 3876 4222 7260 10992 715
Size: 1486 1124 551 346 3038 3732 1011
Fragments arranged by size:
3732 3038 1486 1124 1011 551 346
BsaAI yAC'GTr
Cuts at: 1757 2298 3969 4898 5301 6147 6462 6996 8157
Size: 541 1671 929 403 846 315 534 1161
Cuts at: 8157 8641 1757
Size: 484 4404
Fragments arranged by size:
4404 1671 1161 929 846 541 534 484
403 315
BsaBI GATnn'nnATC
Cuts at: 6 5617 8802 10839 6
Size: 5611 3185 2037 455
Fragments arranged by size:
5611 3185 2037 455
BsaHI Gr'CG_yC
Cuts at: 134 586 683 2231 3863 4361 4531 5387 6227
Size: 452 97 1548 1632 498 170 856 840
Cuts at: 6227 7217 7529 8719 8967 9669 11165 134
Size: 990 312 1190 248 702 1496 257
Fragments arranged by size:
1632 1548 1496 1190 990 856 840 702
498 452 312 257 248 170 97
BsaWI w'CCGG_w
Cuts at: 7666 7900 8477 8998 9676 9950 10097 10508 7666
Size: 234 577 521 678 274 147 411 8446
Fragments arranged by size:
8446 678 577 521 411 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 391 421 520 550 4078 4108 7782 7812 391
Size: 30 99 30 3528 30 3674 30 3867
Fragments arranged by size:
3867 3674 3528 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 1087 1681 3217 4081 5344 5347 5363 5428
Size: 252 594 1536 864 1263 3 16 65
Cuts at: 5428 6085 6745 7477 7950 7992 8161 835
Size: 657 660 732 473 42 169 3962
Fragments arranged by size:
3962 1536 1263 864 732 660 657 594
473 252 169 65 42 16 3
BseYI C'CCAG_C
Cuts at: 629 1141 1690 4464 4551 4626 4710 7032 7138
Size: 512 549 2774 87 75 84 2322 106
Cuts at: 7138 7612 7847 8713 9471 10048 10544 629
Size: 474 235 866 758 577 496 1373
Fragments arranged by size:
2774 2322 1373 866 758 577 549 512
496 474 235 106 87 84 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1027 1404 2213 2839 3859 4069 4305 5506
Size: 519 377 809 626 1020 210 236 1201
Cuts at: 5506 5590 5832 6845 7420 7771 508
Size: 84 242 1013 575 351 4025
Fragments arranged by size:
4025 1201 1020 1013 809 626 575 519
377 351 242 236 210 84
BsiEI CG_ry'CG
Cuts at: 104 3851 6971 8830 8876 10084 104
Size: 3747 3120 1859 46 1208 1308
Fragments arranged by size:
3747 3120 1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 338 440 1166 1634 2030 3986 4453 5177
Size: 84 102 726 468 396 1956 467 724
Cuts at: 5177 5279 5501 6146 7280 7442 8126 8302 9080
Size: 102 222 645 1134 162 684 176 778
Cuts at: 9080 9270 10062 254
Size: 190 792 1480
Fragments arranged by size:
1956 1480 1134 792 778 726 724 684
645 468 467 396 222 190 176 162
102 102 84
BsmI GAATG_Cn'
Cuts at: 849 3061 7707 849
Size: 2212 4646 4430
Fragments arranged by size:
4646 4430 2212
BsmBI CGTCTCn'nnnn_
Cuts at: 5089 7224 7342 7354 7479 5089
Size: 2135 118 12 125 8898
Fragments arranged by size:
8898 2135 125 118 12
Bsp1286I G_dGCh'C
Cuts at: 254 311 338 440 629 803 906 1133 1166
Size: 57 27 102 189 174 103 227 33
Cuts at: 1166 1479 1634 1663 1807 1940 2030 2303 2357
Size: 313 155 29 144 133 90 273 54
Cuts at: 2357 2365 2459 2542 2731 2971 3212 3412 3815
Size: 8 94 83 189 240 241 200 403
Cuts at: 3815 3986 4115 4123 4246 4402 4453 4485 4558
Size: 171 129 8 123 156 51 32 73
Cuts at: 4558 4643 5074 5177 5279 5339 5501 5653 5803
Size: 85 431 103 102 60 162 152 150
Cuts at: 5803 5929 5989 6057 6077 6146 6422 6947 6962
Size: 126 60 68 20 69 276 525 15
Cuts at: 6962 7001 7050 7280 7370 7416 7442 7563 7652
Size: 39 49 230 90 46 26 121 89
Cuts at: 7652 7975 8035 8126 8302 8913 9006 9080 9270
Size: 323 60 91 176 611 93 74 190
Cuts at: 9270 9332 10062 254
Size: 62 730 1480
Fragments arranged by size:
1480 730 611 525 431 403 323 313
276 273 241 240 230 227 200 190
189 189 176 174 171 162 156 155
152 150 144 133 129 126 123 121
103 103 102 102 94 93 91 90
90 89 85 83 74 73 69 68
62 60 60 60 57 54 51 49
46 39 33 32 29 27 26 20
15 8 8
BspEI T'CCGG_A
Cuts at: 7900 10508 7900
Size: 2608 8680
BspHI T'CATG_A
Cuts at: 4716 10464 4716
Size: 5748 5540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 1440 3479 4530 4922 5854 6304 8338 8552
Size: 1147 2039 1051 392 932 450 2034 214
Cuts at: 8552 8854 9235 293
Size: 302 381 2346
Fragments arranged by size:
2346 2039 2034 1147 1051 932 450 392
381 302 214
BsrBI CCG'CTC
Cuts at: 50 2403 3169 4240 9580 9634 11118 11283 50
Size: 2353 766 1071 5340 54 1484 165 55
Fragments arranged by size:
5340 2353 1484 1071 766 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 809 1281 2069 6315 7151 7823 9200 252
Size: 557 472 788 4246 836 672 1377 2340
Fragments arranged by size:
4246 2340 1377 836 788 672 557 472
BsrFI r'CCGG_y
Cuts at: 136 1321 1364 1618 2146 2797 3131 4931 5125
Size: 1185 43 254 528 651 334 1800 194
Cuts at: 5125 6101 6493 7093 7666 7705 9286 9467 9676
Size: 976 392 600 573 39 1581 181 209
Cuts at: 9676 136
Size: 1748
Fragments arranged by size:
1800 1748 1581 1185 976 651 600 573
528 392 334 254 209 194 181 43
39
BsrGI T'GTAC_A
Cuts at: 8180 8180
Size: 11288
BssSI C'ACGA_G
Cuts at: 448 2189 3077 3977 5326 7519 9559 9917 448
Size: 1741 888 900 1349 2193 2040 358 1819
Fragments arranged by size:
2193 2040 1819 1741 1349 900 888 358
BstAPI GCAn_nnn'nTGC
Cuts at: 600 2519 3810 8665 600
Size: 1919 1291 4855 3223
Fragments arranged by size:
4855 3223 1919 1291
BstBI TT'CG_AA
Cuts at: 9649 9649
Size: 11288
BstEII G'GTnAC_C
Cuts at: 1155 1746 3238 3912 6489 6867 1155
Size: 591 1492 674 2577 378 5576
Fragments arranged by size:
5576 2577 1492 674 591 378
BstXI CCAn_nnnn'nTGG
Cuts at: 3442 3529 4566 5644 6596 7158 7525 7570 7935
Size: 87 1037 1078 952 562 367 45 365
Cuts at: 7935 8019 3442
Size: 84 6711
Fragments arranged by size:
6711 1078 1037 952 562 367 365 87
84 45
BstYI r'GATC_y
Cuts at: 1 215 611 2660 3680 3940 4838 4967 6113
Size: 214 396 2049 1020 260 898 129 1146
Cuts at: 6113 6197 6584 6802 7586 8313 8780 8803 9138
Size: 84 387 218 784 727 467 23 335
Cuts at: 9138 9384 10396 10482 10494 10840 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2049 1146 1020 1012 898 784 727 467
449 396 387 346 335 260 246 218
214 129 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 818 7775 818
Size: 6957 4331
BtgI C'CryG_G
Cuts at: 176 205 410 749 1248 1436 2003 3476 4325
Size: 29 205 339 499 188 567 1473 849
Cuts at: 4325 5049 5718 5792 5993 6984 7802 7928 8078
Size: 724 669 74 201 991 818 126 150
Cuts at: 8078 176
Size: 3386
Fragments arranged by size:
3386 1473 991 849 818 724 669 567
499 339 205 201 188 150 126 74
29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 167 303 927 1346 2000 6531 9238 9419 9582
Size: 136 624 419 654 4531 2707 181 163
Cuts at: 9582 167
Size: 1873
Fragments arranged by size:
4531 2707 1873 654 624 419 181 163
136
BtsI GCAGTG_nn'
Cuts at: 1310 2936 8531 1310
Size: 1626 5595 4067
Fragments arranged by size:
5595 4067 1626
ClaI AT'CG_AT
Cuts at: 7541 7541
Size: 11288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1281 1316 6338 6373 6966 7001 7821 7856 1281
Size: 35 5022 35 593 35 820 35 4713
Fragments arranged by size:
5022 4713 820 593 35 35 35 35
DraI TTT'AAA
Cuts at: 8287 8287
Size: 11288
DraIII CAC_nnn'GTG
Cuts at: 1155 3246 3898 6150 7056 7857 1155
Size: 2091 652 2252 906 801 4586
Fragments arranged by size:
4586 2252 2091 906 801 652
DrdI GACnn_nn'nnGTC
Cuts at: 867 4360 7252 8248 8994 9852 867
Size: 3493 2892 996 746 858 2303
Fragments arranged by size:
3493 2892 2303 996 858 746
EaeI y'GGCC_r
Cuts at: 1173 1280 1857 4524 4568 4929 5789 6987 7091
Size: 107 577 2667 44 361 860 1198 104
Cuts at: 7091 8873 9047 9438 9465 10810 1173
Size: 1782 174 391 27 1345 1651
Fragments arranged by size:
2667 1782 1651 1345 1198 860 577 391
361 174 107 104 44 27
EagI C'GGCC_G
Cuts at: 8873 8873
Size: 11288
EarI CTCTTCn'nnn_
Cuts at: 9311 9521 9311
Size: 210 11078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 208 373 3679 6543 9496 9806 9952 11098 208
Size: 165 3306 2864 2953 310 146 1146 398
Fragments arranged by size:
3306 2953 2864 1146 398 310 165 146
EcoICRI GAG'CTC
Cuts at: 1632 5175 5499 8300 1632
Size: 3543 324 2801 4620
Fragments arranged by size:
4620 3543 2801 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 508 1079 1411 2158 2233 3172 3622 4207
Size: 90 571 332 747 75 939 450 585
Cuts at: 4207 4370 5363 5789 5897 6028 6106 7099 7339
Size: 163 993 426 108 131 78 993 240
Cuts at: 7339 7959 9113 9545 10292 10560 418
Size: 620 1154 432 747 268 1146
Fragments arranged by size:
1154 1146 993 993 939 747 747 620
585 571 450 432 426 332 268 240
163 131 108 90 78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 193 3907 193
Size: 3714 7574
EcoO109I rG'GnC_Cy
Cuts at: 186 735 1053 1840 1936 1937 2539 2727 2728
Size: 549 318 787 96 1 602 188 1
Cuts at: 2728 2952 3024 3208 3209 3408 3409 3877 4089
Size: 224 72 184 1 199 1 468 212
Cuts at: 4089 4251 4273 4481 4555 4640 4704 5071 5456
Size: 162 22 208 74 85 64 367 385
Cuts at: 5456 5799 5966 6753 6958 6959 7143 7165 7617
Size: 343 167 787 205 1 184 22 452
Cuts at: 7617 7715 8031 8032 8277 8427 186
Size: 98 316 1 245 150 3047
Fragments arranged by size:
3047 787 787 602 549 468 452 385
367 343 318 316 245 224 212 208
205 199 188 184 184 167 162 150
98 96 85 74 72 64 22 22
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 8292 8292
Size: 11288
EcoRV GAT'ATC
Cuts at: 3225 3225
Size: 11288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2382 2988 4140 8088 9307 2382
Size: 606 1152 3948 1219 4363
Fragments arranged by size:
4363 3948 1219 1152 606
FspI TGC'GCA
Cuts at: 6160 9069 6160
Size: 2909 8379
HaeII r_GCGC'y
Cuts at: 276 686 4534 5233 5390 6093 6627 6798 6926
Size: 410 3848 699 157 703 534 171 128
Cuts at: 6926 7139 8722 8970 9992 10971 276
Size: 213 1583 248 1022 979 593
Fragments arranged by size:
3848 1583 1022 979 703 699 593 534
410 248 213 171 157 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 391 423 3670 3702 3925 3957 5788
Size: 32 202 32 3247 32 223 32 1831
Cuts at: 5788 5820 7726 7758 9130 9160 9162 9192 157
Size: 32 1906 32 1372 30 2 30 2253
Fragments arranged by size:
3247 2253 1906 1831 1372 223 202 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4203 4365 5730 8327 4203
Size: 162 1365 2597 7164
Fragments arranged by size:
7164 2597 1365 162
KasI G'GCGC_C
Cuts at: 682 4530 5386 8718 8966 682
Size: 3848 856 3332 248 3004
Fragments arranged by size:
3848 3332 3004 856 248
KpnI G_GTAC'C
Cuts at: 5210 8308 5210
Size: 3098 8190
MluI A'CGCG_T
Cuts at: 10721 10721
Size: 11288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1493 3668 9959 10143 1493
Size: 2175 6291 184 2638
Fragments arranged by size:
6291 2638 2175 184
MscI TGG'CCA
Cuts at: 1175 1282 1859 6989 9049 1175
Size: 107 577 5130 2060 3414
Fragments arranged by size:
5130 3414 2060 577 107
MslI CAynn'nnrTG
Cuts at: 538 544 1906 2257 2635 2638 2686 2749 2929
Size: 6 1362 351 378 3 48 63 180
Cuts at: 2929 4015 4564 5575 5693 5893 5998 7156 7523
Size: 1086 549 1011 118 200 105 1158 367
Cuts at: 7523 8104 8110 9277 9404 538
Size: 581 6 1167 127 2422
Fragments arranged by size:
2422 1362 1167 1158 1086 1011 581 549
378 367 351 200 180 127 118 105
63 48 6 6 3
MspA1I CmG'CkG
Cuts at: 122 597 633 924 1418 1625 2170 2197 2288
Size: 475 36 291 494 207 545 27 91
Cuts at: 2288 2908 2997 4742 5355 5976 6036 7032 7404
Size: 620 89 1745 613 621 60 996 372
Cuts at: 7404 7755 8399 8713 9073 10086 10331 122
Size: 351 644 314 360 1013 245 1079
Fragments arranged by size:
1745 1079 1013 996 644 621 620 613
545 494 475 372 360 351 314 291
245 207 91 89 60 36 27
NaeI GCC'GGC
Cuts at: 138 1323 1366 1620 2799 3133 7095 7707 9469
Size: 1185 43 254 1179 334 3962 612 1762
Cuts at: 9469 138
Size: 1957
Fragments arranged by size:
3962 1957 1762 1185 1179 612 334 254
43
NarI GG'CG_CC
Cuts at: 683 4531 5387 8719 8967 683
Size: 3848 856 3332 248 3004
Fragments arranged by size:
3848 3332 3004 856 248
NcoI C'CATG_G
Cuts at: 1436 5049 5718 7802 1436
Size: 3613 669 2084 4922
Fragments arranged by size:
4922 3613 2084 669
NgoMIV G'CCGG_C
Cuts at: 136 1321 1364 1618 2797 3131 7093 7705 9467
Size: 1185 43 254 1179 334 3962 612 1762
Cuts at: 9467 136
Size: 1957
Fragments arranged by size:
3962 1957 1762 1185 1179 612 334 254
43
NspI r_CATG'y
Cuts at: 354 1462 3195 5147 6178 6395 8341 8841 9748
Size: 1108 1733 1952 1031 217 1946 500 907
Cuts at: 9748 354
Size: 1894
Fragments arranged by size:
1952 1946 1894 1733 1108 1031 907 500
217
PasI CC'CwG_GG
Cuts at: 1221 2731 4393 4435 5644 8035 1221
Size: 1510 1662 42 1209 2391 4474
Fragments arranged by size:
4474 2391 1662 1510 1209 42
PciI A'CATG_T
Cuts at: 9744 9744
Size: 11288
PflMI CCAn_nnn'nTGG
Cuts at: 750 1757 3163 4842 5055 5304 5402 5724 6954
Size: 1007 1406 1679 213 249 98 322 1230
Cuts at: 6954 750
Size: 5084
Fragments arranged by size:
5084 1679 1406 1230 1007 322 249 213
98
PfoI T'CCnGG_A
Cuts at: 4347 4877 7232 7355 4347
Size: 530 2355 123 8280
Fragments arranged by size:
8280 2355 530 123
PmeI GTTT'AAAC
Cuts at: 8287 8287
Size: 11288
PmlI CAC'GTG
Cuts at: 1757 3969 5301 6147 6462 6996 1757
Size: 2212 1332 846 315 534 6049
Fragments arranged by size:
6049 2212 1332 846 534 315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10427 10459 10427
Size: 32 11256
PpuMI rG'GwC_Cy
Cuts at: 186 735 1053 1840 2952 4089 4273 4704 5966
Size: 549 318 787 1112 1137 184 431 1262
Cuts at: 5966 6753 7165 7617 8277 186
Size: 787 412 452 660 3197
Fragments arranged by size:
3197 1262 1137 1112 787 787 660 549
452 431 412 318 184
PshAI GACnn'nnGTC
Cuts at: 2240 4514 2240
Size: 2274 9014
PspOMI G'GGCC_C
Cuts at: 1936 2538 2727 3208 3408 4481 4554 4639 5070
Size: 602 189 481 200 1073 73 85 431
Cuts at: 5070 5799 6958 8031 1936
Size: 729 1159 1073 5193
Fragments arranged by size:
5193 1159 1073 1073 729 602 481 431
200 189 85 73
PspXI vC'TCGA_Gb
Cuts at: 328 418 427 7651 328
Size: 90 9 7224 3965
Fragments arranged by size:
7224 3965 90 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 346 378 8164 8196 346
Size: 32 7786 32 3438
Fragments arranged by size:
7786 3438 32 32
PstI C_TGCA'G
Cuts at: 290 989 1562 2482 2547 4516 4541 8089 8335
Size: 699 573 920 65 1969 25 3548 246
Cuts at: 8335 9020 290
Size: 685 2558
Fragments arranged by size:
3548 2558 1969 920 699 685 573 246
65 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11288
PvuII CAG'CTG
Cuts at: 633 924 2197 5355 5976 6036 7404 7755 8713
Size: 291 1273 3158 621 60 1368 351 958
Cuts at: 8713 9073 633
Size: 360 2848
Fragments arranged by size:
3158 2848 1368 1273 958 621 360 351
291 60
RsrII CG'GwC_CG
Cuts at: 9483 9483
Size: 11288
SacI G_AGCT'C
Cuts at: 1634 5177 5501 8302 1634
Size: 3543 324 2801 4620
Fragments arranged by size:
4620 3543 2801 324
SalI G'TCGA_C
Cuts at: 8325 8325
Size: 11288
SanDI GG'GwC_CC
Cuts at: 735 2952 4273 6753 7617 735
Size: 2217 1321 2480 864 4406
Fragments arranged by size:
4406 2480 2217 1321 864
SapI GCTCTTCn'nnn_
Cuts at: 9311 9521 9311
Size: 210 11078
SbfI CC_TGCA'GG
Cuts at: 4541 8335 4541
Size: 3794 7494
ScaI AGT'ACT
Cuts at: 1717 10805 1717
Size: 9088 2200
SexAI A'CCwGG_T
Cuts at: 897 5465 7239 897
Size: 4568 1774 4946
Fragments arranged by size:
4946 4568 1774
SfcI C'TryA_G
Cuts at: 33 286 985 1558 2478 2543 3753 4512 4537
Size: 253 699 573 920 65 1210 759 25
Cuts at: 4537 5883 6927 7980 8085 8331 9016 10009 10200
Size: 1346 1044 1053 105 246 685 993 191
Cuts at: 10200 33
Size: 1121
Fragments arranged by size:
1346 1210 1121 1053 1044 993 920 759
699 685 573 253 246 191 105 65
25
SfoI GGC'GCC
Cuts at: 684 4532 5388 8720 8968 684
Size: 3848 856 3332 248 3004
Fragments arranged by size:
3848 3332 3004 856 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11288
SmaI CCC'GGG
Cuts at: 763 3317 4486 5921 7126 7417 7435 8310 763
Size: 2554 1169 1435 1205 291 18 875 3741
Fragments arranged by size:
3741 2554 1435 1205 1169 875 291 18
SmlI C'TyrA_G
Cuts at: 256 328 418 427 1633 2443 3322 6580 7369
Size: 72 90 9 1206 810 879 3258 789
Cuts at: 7369 7651 7962 8443 9358 9850 10112 256
Size: 282 311 481 915 492 262 1432
Fragments arranged by size:
3258 1432 1206 915 879 810 789 492
481 311 282 262 90 72 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11288
SphI G_CATG'C
Cuts at: 8341 8841 8341
Size: 500 10788
StuI AGG'CCT
Cuts at: 730 1556 2122 2441 4184 4751 5603 5711 8050
Size: 826 566 319 1743 567 852 108 2339
Cuts at: 8050 730
Size: 3968
Fragments arranged by size:
3968 2339 1743 852 826 567 566 319
108
StyI C'CwwG_G
Cuts at: 881 1133 1436 1565 1610 2789 2837 3296 3368
Size: 252 303 129 45 1179 48 459 72
Cuts at: 3368 4254 5049 5117 5718 5807 6128 7745 7802
Size: 886 795 68 601 89 321 1617 57
Cuts at: 7802 7877 8422 8776 881
Size: 75 545 354 3393
Fragments arranged by size:
3393 1617 1179 886 795 601 545 459
354 321 303 252 129 89 75 72
68 57 48 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 2785 4156 4621 5047 5604 6754 7024 8871
Size: 2490 1371 465 426 557 1150 270 1847
Cuts at: 8871 9004 9463 9672 295
Size: 133 459 209 1911
Fragments arranged by size:
2490 1911 1847 1371 1150 557 465 459
426 270 209 133
TatI w'GTAC_w
Cuts at: 1715 2603 2900 3491 4052 4331 8180 10803 1715
Size: 888 297 591 561 279 3849 2623 2200
Fragments arranged by size:
3849 2623 2200 888 591 561 297 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 190 222 1476 1508 3884 3916 7782 7814 7909
Size: 32 1254 32 2376 32 3866 32 95
Cuts at: 7909 7941 10805 10837 190
Size: 32 2864 32 641
Fragments arranged by size:
3866 2864 2376 1254 641 95 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2956 7953 9085 2956
Size: 4997 1132 5159
Fragments arranged by size:
5159 4997 1132
XbaI T'CTAG_A
Cuts at: 8319 8319
Size: 11288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 747 3636 4782 7752 7935 8109 8783 747
Size: 2889 1146 2970 183 174 674 3252
Fragments arranged by size:
3252 2970 2889 1146 674 183 174
XhoI C'TCGA_G
Cuts at: 328 418 427 7651 328
Size: 90 9 7224 3965
Fragments arranged by size:
7224 3965 90 9
XmaI C'CCGG_G
Cuts at: 761 3315 4484 5919 7124 7415 7433 8308 761
Size: 2554 1169 1435 1205 291 18 875 3741
Fragments arranged by size:
3741 2554 1435 1205 1169 875 291 18
XmnI GAAnn'nnTTC
Cuts at: 229 8071 229
Size: 7842 3446
ZraI GAC'GTC
Cuts at: 4362 4362
Size: 11288
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BfrBI BmtI
BsiWI BssHII BstZ17I FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI