Restriction map for
pF1KE2351
(Circular) (Six-base) MAPSORT of: pF1KE2351.seq Check: 1317 from: 1 to: 4517
pF1KE2351 4517 bp
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 491 550 575 650 1007 1300 1363 1470
Size: 121 59 25 75 357 293 63 107
Cuts at: 1470 2342 2774 3521 3789 370
Size: 872 432 747 268 1098
Fragments arranged by size:
1098 872 747 432 357 293 268 121
107 75 63 59 25
AfeI AGC'GCT
Cuts at: 667 667
Size: 4517
AflII C'TTAA_G
Cuts at: 1009 1009
Size: 4517
AflIII A'CryG_T
Cuts at: 1869 2973 3950 1869
Size: 1104 977 2436
Fragments arranged by size:
2436 1104 977
AgeI A'CCGG_T
Cuts at: 2905 2905
Size: 4517
AhdI GACnn_n'nnGTC
Cuts at: 621 621
Size: 4517
AleI CACnn'nnGTG
Cuts at: 207 207
Size: 4517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 225 901 911 225
Size: 676 10 3831
Fragments arranged by size:
3831 676 10
AlwNI CAG_nnn'CTG
Cuts at: 1822 3389 1822
Size: 1567 2950
ApaLI G'TGCA_C
Cuts at: 3287 3287
Size: 4517
ApoI r'AATT_y
Cuts at: 955 977 1109 1188 1402 1454 1521 3870 4126
Size: 22 132 79 214 52 67 2349 256
Cuts at: 4126 955
Size: 1346
Fragments arranged by size:
2349 1346 256 214 132 79 67 52
22
AseI AT'TA_AT
Cuts at: 20 881 20
Size: 861 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 165 1537 165
Size: 1372 3145
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 145 1533 1947 2195 2230 145
Size: 1388 414 248 35 2432
Fragments arranged by size:
2432 1388 414 248 35
BanII G_rGCy'C
Cuts at: 1531 2561 1531
Size: 1030 3487
BbeI G_GCGC'C
Cuts at: 149 1951 2199 149
Size: 1802 248 2467
Fragments arranged by size:
2467 1802 248
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3176 4517 2415
Size: 761 1341 2415
Fragments arranged by size:
2415 1341 761
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 4517
BglII A'GATC_T
Cuts at: 1346 2032 4069 1346
Size: 686 2037 1794
Fragments arranged by size:
2037 1794 686
BlpI GC'TnA_GC
Cuts at: 160 1629 160
Size: 1469 3048
Bme1580I G_kGCm'C
Cuts at: 1336 2142 2235 3291 1336
Size: 806 93 1056 2562
Fragments arranged by size:
2562 1056 806 93
BmrI ACTGGGnnnn_n'
Cuts at: 1729 1810 2145 4289 1729
Size: 81 335 2144 1957
Fragments arranged by size:
2144 1957 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 4517
Bpu10I CC'TnA_GC
Cuts at: 662 662
Size: 4517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2572 3064 3362 1693
Size: 879 492 298 2848
Fragments arranged by size:
2848 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 829 910 4221 829
Size: 81 3311 1125
Fragments arranged by size:
3311 1125 81
BsaAI yAC'GTr
Cuts at: 1870 1870
Size: 4517
BsaBI GATnn'nnATC
Cuts at: 6 2031 4068 6
Size: 2025 2037 455
Fragments arranged by size:
2037 2025 455
BsaHI Gr'CG_yC
Cuts at: 146 1948 2196 2898 4394 146
Size: 1802 248 702 1496 269
Fragments arranged by size:
1802 1496 702 269 248
BsaWI w'CCGG_w
Cuts at: 1706 2227 2905 3179 3326 3737 1706
Size: 521 678 274 147 411 2486
Fragments arranged by size:
2486 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 193
Size: 4517
BseYI C'CCAG_C
Cuts at: 119 1201 1942 2700 3277 3773 119
Size: 1082 741 758 577 496 863
Fragments arranged by size:
1082 863 758 741 577 496
BsiEI CG_ry'CG
Cuts at: 104 2059 2105 3313 104
Size: 1955 46 1208 1308
Fragments arranged by size:
1955 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1531 2309 2499 3291 1531
Size: 778 190 792 2757
Fragments arranged by size:
2757 792 778 190
BsmI GAATG_Cn'
Cuts at: 844 844
Size: 4517
Bsp1286I G_dGCh'C
Cuts at: 1336 1531 2142 2235 2309 2499 2561 3291 1336
Size: 195 611 93 74 190 62 730 2562
Fragments arranged by size:
2562 730 611 195 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3737 3737
Size: 4517
BspHI T'CATG_A
Cuts at: 1152 3693 1152
Size: 2541 1976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1567 1781 2083 2464 1567
Size: 214 302 381 3620
Fragments arranged by size:
3620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2809 2863 4347 4512 50
Size: 2759 54 1484 165 55
Fragments arranged by size:
2759 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2429 2429
Size: 4517
BsrFI r'CCGG_y
Cuts at: 2515 2696 2905 2515
Size: 181 209 4127
Fragments arranged by size:
4127 209 181
BssSI C'ACGA_G
Cuts at: 2788 3146 2788
Size: 358 4159
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 1894
Size: 4517
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 226 695 226
Size: 469 4048
BstYI r'GATC_y
Cuts at: 1 1346 1542 2009 2032 2367 2613 3625 3711
Size: 1345 196 467 23 335 246 1012 86
Cuts at: 3711 3723 4069 1
Size: 12 346 449
Fragments arranged by size:
1345 1012 467 449 346 335 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 107 114 171 107
Size: 7 57 4453
Fragments arranged by size:
4453 57 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 2648 2811 2467
Size: 181 163 4173
Fragments arranged by size:
4173 181 163
BtsI GCAGTG_nn'
Cuts at: 530 1334 1760 530
Size: 804 426 3287
Fragments arranged by size:
3287 804 426
DraI TTT'AAA
Cuts at: 761 1293 1516 761
Size: 532 223 3762
Fragments arranged by size:
3762 532 223
DrdI GACnn_nn'nnGTC
Cuts at: 406 2223 3081 406
Size: 1817 858 1842
Fragments arranged by size:
1842 1817 858
EaeI y'GGCC_r
Cuts at: 2102 2276 2667 2694 4039 2102
Size: 174 391 27 1345 2580
Fragments arranged by size:
2580 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 346 788 2540 2750 346
Size: 442 1752 210 2113
Fragments arranged by size:
2113 1752 442 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2725 3035 3181 4327 2725
Size: 310 146 1146 2915
Fragments arranged by size:
2915 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1529 1529
Size: 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 491 550 575 650 1007 1300 1363 1470
Size: 121 59 25 75 357 293 63 107
Cuts at: 1470 2342 2774 3521 3789 370
Size: 872 432 747 268 1098
Fragments arranged by size:
1098 872 747 432 357 293 268 121
107 75 63 59 25
EcoO109I rG'GnC_Cy
Cuts at: 1656 1656
Size: 4517
EcoRI G'AATT_C
Cuts at: 1188 1402 1521 1188
Size: 214 119 4184
Fragments arranged by size:
4184 214 119
EcoRV GAT'ATC
Cuts at: 904 904
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1254 2536 1254
Size: 1282 3235
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 149 669 1080 1951 2199 3221 4200 149
Size: 520 411 871 248 1022 979 466
Fragments arranged by size:
1022 979 871 520 466 411 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 345 377 607 639 2359 2389 2391 2421 345
Size: 32 230 32 1720 30 2 30 2441
Fragments arranged by size:
2441 1720 230 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
HindIII A'AGCT_T
Cuts at: 1312 1312
Size: 4517
KasI G'GCGC_C
Cuts at: 145 1947 2195 145
Size: 1802 248 2467
Fragments arranged by size:
2467 1802 248
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3188 3372 3188
Size: 184 4333
MscI TGG'CCA
Cuts at: 2278 2278
Size: 4517
MslI CAynn'nnrTG
Cuts at: 207 883 893 1181 2506 2633 207
Size: 676 10 288 1325 127 2091
Fragments arranged by size:
2091 1325 676 288 127 10
MspA1I CmG'CkG
Cuts at: 116 199 1628 1942 2302 3315 3560 116
Size: 83 1429 314 360 1013 245 1073
Fragments arranged by size:
1429 1073 1013 360 314 245 83
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 4517
NarI GG'CG_CC
Cuts at: 146 1948 2196 146
Size: 1802 248 2467
Fragments arranged by size:
2467 1802 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4517
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 4517
NspI r_CATG'y
Cuts at: 874 1570 2070 2977 874
Size: 696 500 907 2414
Fragments arranged by size:
2414 907 696 500
PciI A'CATG_T
Cuts at: 2973 2973
Size: 4517
PflMI CCAn_nnn'nTGG
Cuts at: 1387 1387
Size: 4517
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4485
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 790 822 790
Size: 32 4485
PstI C_TGCA'G
Cuts at: 1564 2249 1564
Size: 685 3832
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 1942 2302 1942
Size: 360 4157
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 1531 1531
Size: 4517
SacII CC_GC'GG
Cuts at: 117 117
Size: 4517
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SexAI A'CCwGG_T
Cuts at: 919 919
Size: 4517
SfcI C'TryA_G
Cuts at: 33 282 849 1560 2245 3238 3429 33
Size: 249 567 711 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 567 249 191
SfoI GGC'GCC
Cuts at: 147 1949 2197 147
Size: 1802 248 2467
Fragments arranged by size:
2467 1802 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 1009 1672 2587 3079 3341 1009
Size: 663 915 492 262 2185
Fragments arranged by size:
2185 915 663 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
SspI AAT'ATT
Cuts at: 1260 1506 1260
Size: 246 4271
StyI C'CwwG_G
Cuts at: 107 1651 2005 107
Size: 1544 354 2619
Fragments arranged by size:
2619 1544 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2100 2233 2692 2901 2100
Size: 133 459 209 3716
Fragments arranged by size:
3716 459 209 133
TatI w'GTAC_w
Cuts at: 4032 4032
Size: 4517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4034 4066 4034
Size: 32 4485
Tth111I GACn'n_nGTC
Cuts at: 2314 2314
Size: 4517
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2012 2012
Size: 4517
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
XmnI GAAnn'nnTTC
Cuts at: 1148 1260 1455 1148
Size: 112 195 4210
Fragments arranged by size:
4210 195 112
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AloI ApaI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI