Restriction map for
pF1KE2349
(Circular) (Six-base) MAPSORT of: pF1KE2349.seq Check: 9767 from: 1 to: 4055
pF1KE2349 4055 bp
With 182 enzymes: SgfI *
May 20, 2011 15:42 ..
AccI GT'mk_AC
Cuts at: 299 1093 299
Size: 794 3261
Acc65I G'GTAC_C
Cuts at: 913 1071 913
Size: 158 3897
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1880 2312 3059 3327 604
Size: 1276 432 747 268 1332
Fragments arranged by size:
1332 1276 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 230 376 482 230
Size: 146 106 3803
Fragments arranged by size:
3803 146 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 123 728 750 820 1360 2927 123
Size: 605 22 70 540 1567 1251
Fragments arranged by size:
1567 1251 605 540 70 22
ApaI G_GGCC'C
Cuts at: 137 137
Size: 4055
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 1059 3408 3664 1059
Size: 2349 256 1450
Fragments arranged by size:
2349 1450 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 129 1075 129
Size: 946 3109
AvrII C'CTAG_G
Cuts at: 642 642
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 446 464 553 787 913 1071 1485 1733 1768
Size: 18 89 234 126 158 414 248 35
Cuts at: 1768 446
Size: 2733
Fragments arranged by size:
2733 414 248 234 158 126 89 35
18
BanII G_rGCy'C
Cuts at: 115 137 247 951 1069 2099 115
Size: 22 110 704 118 1030 2071
Fragments arranged by size:
2071 1030 704 118 110 22
BbeI G_GCGC'C
Cuts at: 450 1489 1737 450
Size: 1039 248 2768
Fragments arranged by size:
2768 1039 248
BbvCI CC'TCA_GC
Cuts at: 698 698
Size: 4055
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 132 132
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 721 1167 721
Size: 446 3609
Bme1580I G_kGCm'C
Cuts at: 137 237 1680 1773 2829 137
Size: 100 1443 93 1056 1363
Fragments arranged by size:
1443 1363 1056 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 988 1267 1348 1683 3827 988
Size: 279 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 613 665 914 965 259
Size: 354 52 249 51 3349
Fragments arranged by size:
3349 354 249 52 51
Bpu10I CC'TnA_GC
Cuts at: 698 698
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 732 3759 732
Size: 3027 1028
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 396 447 1486 1734 2436 3932 396
Size: 51 1039 248 702 1496 519
Fragments arranged by size:
1496 1039 702 519 248 51
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 264 292 294 322 264
Size: 28 2 28 3997
Fragments arranged by size:
3997 28 28 2
BseYI C'CCAG_C
Cuts at: 188 528 892 1480 2238 2815 3311 188
Size: 340 364 588 758 577 496 932
Fragments arranged by size:
932 758 588 577 496 364 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4055
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 373 546 1069 1847 2037 2829 373
Size: 173 523 778 190 792 1599
Fragments arranged by size:
1599 792 778 523 190 173
Bsp1286I G_dGCh'C
Cuts at: 115 137 237 247 373 546 951 1069 1680
Size: 22 100 10 126 173 405 118 611
Cuts at: 1680 1773 1847 2037 2099 2829 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 405 190 173 126 118
100 93 74 62 22 10
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 1105 1319 1621 2002 434
Size: 671 214 302 381 2487
Fragments arranged by size:
2487 671 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 123 1432 123
Size: 1309 2746
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 484 484
Size: 4055
BstXI CCAn_nnnn'nTGG
Cuts at: 461 546 461
Size: 85 3970
BstYI r'GATC_y
Cuts at: 1 921 1080 1547 1570 1905 2151 3163 3249
Size: 920 159 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 159
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4055
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 575 857 1298 575
Size: 282 441 3332
Fragments arranged by size:
3332 441 282
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DraIII CAC_nnn'GTG
Cuts at: 292 292
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 419 869 989 1034 1640 1814 2205 2232 3577
Size: 450 120 45 606 174 391 27 1345
Cuts at: 3577 419
Size: 897
Fragments arranged by size:
1345 897 606 450 391 174 120 45
27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 625 2078 2288 625
Size: 1453 210 2392
Fragments arranged by size:
2392 1453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 604 613 665 914 965 1880 2312 3059
Size: 345 9 52 249 51 915 432 747
Cuts at: 3059 3327 259
Size: 268 987
Fragments arranged by size:
987 915 747 432 345 268 249 52
51 9
EcoNI CCTnn'n_nnAGG
Cuts at: 822 822
Size: 4055
EcoO109I rG'GnC_Cy
Cuts at: 120 133 588 747 939 1194 120
Size: 13 455 159 192 255 2981
Fragments arranged by size:
2981 455 255 192 159 13
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 450 1489 1737 2759 3738 450
Size: 1039 248 1022 979 767
Fragments arranged by size:
1039 1022 979 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 348 380 1897 1927 1929 1959 292
Size: 32 24 32 1517 30 2 30 2388
Fragments arranged by size:
2388 1517 32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 446 1485 1733 446
Size: 1039 248 2768
Fragments arranged by size:
2768 1039 248
KpnI G_GTAC'C
Cuts at: 917 1075 917
Size: 158 3897
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 421 871 991 1036 1816 421
Size: 450 120 45 780 2660
Fragments arranged by size:
2660 780 450 120 45
MslI CAynn'nnrTG
Cuts at: 230 376 482 2044 2171 230
Size: 146 106 1562 127 2114
Fragments arranged by size:
2114 1562 146 127 106
MspA1I CmG'CkG
Cuts at: 376 1166 1480 1840 2853 3098 376
Size: 790 314 360 1013 245 1333
Fragments arranged by size:
1333 1013 790 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 447 1486 1734 447
Size: 1039 248 2768
Fragments arranged by size:
2768 1039 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PpuMI rG'GwC_Cy
Cuts at: 939 939
Size: 4055
PspOMI G'GGCC_C
Cuts at: 133 133
Size: 4055
PstI C_TGCA'G
Cuts at: 340 348 1102 1787 340
Size: 8 754 685 2608
Fragments arranged by size:
2608 754 685 8
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 376 1480 1840 376
Size: 1104 360 2591
Fragments arranged by size:
2591 1104 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 625 2078 2288 625
Size: 1453 210 2392
Fragments arranged by size:
2392 1453 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 336 344 636 1098 1783 2776 2967 33
Size: 303 8 292 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 303 292 191 8
SfoI GGC'GCC
Cuts at: 448 1487 1735 448
Size: 1039 248 2768
Fragments arranged by size:
2768 1039 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 131 1077 131
Size: 946 3109
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 886 67
Size: 819 3236
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StuI AGG'CCT
Cuts at: 334 780 334
Size: 446 3609
StyI C'CwwG_G
Cuts at: 107 194 625 642 897 917 1001 1189 1543
Size: 87 431 17 255 20 84 188 354
Cuts at: 1543 107
Size: 2619
Fragments arranged by size:
2619 431 354 255 188 87 84 20
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 1638 1771 2230 2439 750
Size: 888 133 459 209 2366
Fragments arranged by size:
2366 888 459 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 3572 3604 264
Size: 32 3276 32 715
Fragments arranged by size:
3276 715 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 864 999 1550 864
Size: 135 551 3369
Fragments arranged by size:
3369 551 135
XmaI C'CCGG_G
Cuts at: 129 1075 129
Size: 946 3109
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BbsI BcgI BfrBI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI