Restriction map for
pF1KE2342
(Circular) (Six-base) MAPSORT of: pF1KE2342.seq Check: 6213 from: 1 to: 4382
pF1KE2342 4382 bp
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1171 1171
Size: 4382
AatII G_ACGT'C
Cuts at: 719 719
Size: 4382
AccI GT'mk_AC
Cuts at: 926 1127 1420 926
Size: 201 293 3888
Fragments arranged by size:
3888 293 201
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 2207 2639 3386 3654 262
Size: 1945 432 747 268 990
Fragments arranged by size:
1945 990 747 432 268
AflIII A'CryG_T
Cuts at: 1091 1734 2838 3815 1091
Size: 643 1104 977 1658
Fragments arranged by size:
1658 1104 977 643
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 391 619 1687 3254 391
Size: 228 1068 1567 1519
Fragments arranged by size:
1567 1519 1068 228
ApaI G_GGCC'C
Cuts at: 195 195
Size: 4382
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 343 1386 3735 3991 343
Size: 1043 2349 256 734
Fragments arranged by size:
2349 1043 734 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 455 764 1402 455
Size: 309 638 3435
Fragments arranged by size:
3435 638 309
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 487 1398 1812 2060 2095 487
Size: 911 414 248 35 2774
Fragments arranged by size:
2774 911 414 248 35
BanII G_rGCy'C
Cuts at: 195 479 591 608 850 989 1396 2426 195
Size: 284 112 17 242 139 407 1030 2151
Fragments arranged by size:
2151 1030 407 284 242 139 112 17
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 881 881
Size: 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 108 142 108
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 566 1902 566
Size: 1336 3046
BglI GCCn_nnn'nGGC
Cuts at: 298 984 298
Size: 686 3696
BglII A'GATC_T
Cuts at: 741 1897 3934 741
Size: 1156 2037 1189
Fragments arranged by size:
2037 1189 1156
BlpI GC'TnA_GC
Cuts at: 232 1494 232
Size: 1262 3120
Bme1580I G_kGCm'C
Cuts at: 195 492 2007 2100 3156 195
Size: 297 1515 93 1056 1421
Fragments arranged by size:
1515 1421 1056 297 93
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 445 973 171
Size: 274 528 3580
Fragments arranged by size:
3580 528 274
Bpu10I CC'TnA_GC
Cuts at: 1169 1169
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 930 1029 1735 930
Size: 99 706 3577
Fragments arranged by size:
3577 706 99
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 716 1813 2061 2763 4259 716
Size: 1097 248 702 1496 839
Fragments arranged by size:
1496 1097 839 702 248
BsaWI w'CCGG_w
Cuts at: 463 1571 2092 2770 3044 3191 3602 463
Size: 1108 521 678 274 147 411 1243
Fragments arranged by size:
1243 1108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 460 253
Size: 207 4175
BseYI C'CCAG_C
Cuts at: 129 185 357 834 1318 1807 2565 3142 3638
Size: 56 172 477 484 489 758 577 496
Cuts at: 3638 129
Size: 873
Fragments arranged by size:
873 758 577 496 489 484 477 172
56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1199 1009
Size: 190 4192
BsiEI CG_ry'CG
Cuts at: 104 163 315 1924 1970 3178 104
Size: 59 152 1609 46 1208 1308
Fragments arranged by size:
1609 1308 1208 152 59 46
BsiHKAI G_wGCw'C
Cuts at: 287 1396 2174 2364 3156 287
Size: 1109 778 190 792 1513
Fragments arranged by size:
1513 1109 792 778 190
BsmI GAATG_Cn'
Cuts at: 1232 1232
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 195 287 479 492 591 608 850 989 1396
Size: 92 192 13 99 17 242 139 407
Cuts at: 1396 2007 2100 2174 2364 2426 3156 195
Size: 611 93 74 190 62 730 1421
Fragments arranged by size:
1421 730 611 407 242 192 190 139
99 93 92 74 62 17 13
BspEI T'CCGG_A
Cuts at: 463 3602 463
Size: 3139 1243
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 1031 1171 1432 1646 1948 2329 344
Size: 687 140 261 214 302 381 2397
Fragments arranged by size:
2397 687 381 302 261 214 140
BsrBI CCG'CTC
Cuts at: 50 957 2674 2728 4212 4377 50
Size: 907 1717 54 1484 165 55
Fragments arranged by size:
1717 1484 907 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 531 1158 1257 2294 327
Size: 204 627 99 1037 2415
Fragments arranged by size:
2415 1037 627 204 99
BsrFI r'CCGG_y
Cuts at: 172 2380 2561 2770 172
Size: 2208 181 209 1784
Fragments arranged by size:
2208 1784 209 181
BsrGI T'GTAC_A
Cuts at: 1094 1094
Size: 4382
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 903 903
Size: 4382
BstYI r'GATC_y
Cuts at: 1 741 1407 1874 1897 2232 2478 3490 3576
Size: 740 666 467 23 335 246 1012 86
Cuts at: 3576 3588 3934 1
Size: 12 346 449
Fragments arranged by size:
1012 740 666 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 209 263 1274 107
Size: 102 54 1011 3215
Fragments arranged by size:
3215 1011 102 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1163 2332 2513 2676 1163
Size: 1169 181 163 2869
Fragments arranged by size:
2869 1169 181 163
BtsI GCAGTG_nn'
Cuts at: 476 1163 1625 476
Size: 687 462 3233
Fragments arranged by size:
3233 687 462
ClaI AT'CG_AT
Cuts at: 311 311
Size: 4382
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 160 593 968 1044 1056 1967 2141 2532 2559
Size: 433 375 76 12 911 174 391 27
Cuts at: 2559 3904 160
Size: 1345 638
Fragments arranged by size:
1345 911 638 433 391 375 174 76
27 12
EagI C'GGCC_G
Cuts at: 160 1967 160
Size: 1807 2575
EarI CTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 262 445 973 2207 2639 3386 3654 171
Size: 91 183 528 1234 432 747 268 899
Fragments arranged by size:
1234 899 747 528 432 268 183 91
EcoO109I rG'GnC_Cy
Cuts at: 976 1521 976
Size: 545 3837
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
EcoRV GAT'ATC
Cuts at: 452 452
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 436 468 613 645 2224 2254 2256
Size: 32 299 32 145 32 1579 30 2
Cuts at: 2256 2286 105
Size: 30 2201
Fragments arranged by size:
2201 1579 299 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 3053 3237 521
Size: 2532 184 1666
Fragments arranged by size:
2532 1666 184
MscI TGG'CCA
Cuts at: 595 1046 2143 595
Size: 451 1097 2834
Fragments arranged by size:
2834 1097 451
MslI CAynn'nnrTG
Cuts at: 2371 2498 2371
Size: 127 4255
MspA1I CmG'CkG
Cuts at: 189 357 1201 1493 1807 2167 3180 3425 189
Size: 168 844 292 314 360 1013 245 1146
Fragments arranged by size:
1146 1013 844 360 314 292 245 168
NaeI GCC'GGC
Cuts at: 174 2563 174
Size: 2389 1993
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 263 1274 107
Size: 156 1011 3215
Fragments arranged by size:
3215 1011 156
NgoMIV G'CCGG_C
Cuts at: 172 2561 172
Size: 2389 1993
NotI GC'GGCC_GC
Cuts at: 160 160
Size: 4382
NspI r_CATG'y
Cuts at: 433 1095 1435 1935 2842 433
Size: 662 340 500 907 1973
Fragments arranged by size:
1973 907 662 500 340
PciI A'CATG_T
Cuts at: 1091 2838 1091
Size: 1747 2635
PfoI T'CCnGG_A
Cuts at: 1001 1001
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 1029 1029
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PspOMI G'GGCC_C
Cuts at: 191 191
Size: 4382
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 315 104
Size: 211 4171
PvuII CAG'CTG
Cuts at: 357 1201 1807 2167 357
Size: 844 606 360 2572
Fragments arranged by size:
2572 844 606 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 298 298
Size: 4382
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 1537 2452 2944 3206 1537
Size: 915 492 262 2713
Fragments arranged by size:
2713 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StuI AGG'CCT
Cuts at: 1144 1144
Size: 4382
StyI C'CwwG_G
Cuts at: 107 263 296 1139 1274 1516 1870 107
Size: 156 33 843 135 242 354 2619
Fragments arranged by size:
2619 843 354 242 156 135 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1965 2098 2557 2766 1965
Size: 133 459 209 3581
Fragments arranged by size:
3581 459 209 133
TatI w'GTAC_w
Cuts at: 578 1094 3897 578
Size: 516 2803 1063
Fragments arranged by size:
2803 1063 516
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 1877 741
Size: 1136 3246
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
ZraI GAC'GTC
Cuts at: 717 717
Size: 4382
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PciI PfoI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PasI PflMI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI