Restriction map for
pF1KE2341
(Circular) (Six-base) MAPSORT of: pF1KE2341.seq Check: 4190 from: 1 to: 5321
pF1KE2341 5321 bp
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AccI GT'mk_AC
Cuts at: 1060 2359 1060
Size: 1299 4022
Acc65I G'GTAC_C
Cuts at: 2337 2337
Size: 5321
AclI AA'CG_TT
Cuts at: 5161 5161
Size: 5321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 746 766 1925 1957 2269 3146 3578 4325
Size: 198 20 1159 32 312 877 432 747
Cuts at: 4325 4593 548
Size: 268 1276
Fragments arranged by size:
1276 1159 877 747 432 312 268 198
32 20
AflIII A'CryG_T
Cuts at: 2673 3777 4754 2673
Size: 1104 977 3240
Fragments arranged by size:
3240 1104 977
AgeI A'CCGG_T
Cuts at: 3709 3709
Size: 5321
AlwNI CAG_nnn'CTG
Cuts at: 2626 4193 2626
Size: 1567 3754
ApaLI G'TGCA_C
Cuts at: 4091 4091
Size: 5321
ApoI r'AATT_y
Cuts at: 410 633 1192 1286 1856 2325 4674 4930 410
Size: 223 559 94 570 469 2349 256 801
Fragments arranged by size:
2349 801 570 559 469 256 223 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 5321
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
AvaI C'yCGr_G
Cuts at: 2341 2341
Size: 5321
BamHI G'GATC_C
Cuts at: 1176 1414 2346 1176
Size: 238 932 4151
Fragments arranged by size:
4151 932 238
BanI G'GyrC_C
Cuts at: 762 2337 2751 2999 3034 762
Size: 1575 414 248 35 3049
Fragments arranged by size:
3049 1575 414 248 35
BanII G_rGCy'C
Cuts at: 115 2335 3365 115
Size: 2220 1030 2071
Fragments arranged by size:
2220 2071 1030
BbeI G_GCGC'C
Cuts at: 2755 3003 2755
Size: 248 5073
BbsI GAAGACnn'nnnn_
Cuts at: 364 455 610 364
Size: 91 155 5075
Fragments arranged by size:
5075 155 91
BbvCI CC'TCA_GC
Cuts at: 121 121
Size: 5321
BciVI GTATCCnnnnn_n'
Cuts at: 979 3219 3980 5321 979
Size: 2240 761 1341 979
Fragments arranged by size:
2240 1341 979 761
BclI T'GATC_A
Cuts at: 2841 2841
Size: 5321
BfrBI ATG'CAT
Cuts at: 1909 1909
Size: 5321
BglII A'GATC_T
Cuts at: 729 932 2836 4873 729
Size: 203 1904 2037 1177
Fragments arranged by size:
2037 1904 1177 203
BlpI GC'TnA_GC
Cuts at: 2272 2433 2272
Size: 161 5160
Bme1580I G_kGCm'C
Cuts at: 2946 3039 4095 2946
Size: 93 1056 4172
Fragments arranged by size:
4172 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 462 2533 2614 2949 5093 462
Size: 2071 81 335 2144 690
Fragments arranged by size:
2144 2071 690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3688 3688
Size: 5321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1103 1526 1907 1103
Size: 423 381 4517
Fragments arranged by size:
4517 423 381
Bpu10I CC'TnA_GC
Cuts at: 121 264 121
Size: 143 5178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 3376 3868 4166 2497
Size: 879 492 298 3652
Fragments arranged by size:
3652 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5025 5025
Size: 5321
BsaAI yAC'GTr
Cuts at: 2674 2674
Size: 5321
BsaBI GATnn'nnATC
Cuts at: 6 2835 4872 6
Size: 2829 2037 455
Fragments arranged by size:
2829 2037 455
BsaHI Gr'CG_yC
Cuts at: 2004 2752 3000 3702 5198 2004
Size: 748 248 702 1496 2127
Fragments arranged by size:
2127 1496 748 702 248
BsaWI w'CCGG_w
Cuts at: 1417 2510 3031 3709 3983 4130 4541 1417
Size: 1093 521 678 274 147 411 2197
Fragments arranged by size:
2197 1093 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 197 227 335 365 669 699 860 890 1880
Size: 30 108 30 304 30 161 30 990
Cuts at: 1880 1910 197
Size: 30 3608
Fragments arranged by size:
3608 990 304 161 108 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 814 1040 218
Size: 596 226 4499
Fragments arranged by size:
4499 596 226
BseYI C'CCAG_C
Cuts at: 851 2746 3504 4081 4577 851
Size: 1895 758 577 496 1595
Fragments arranged by size:
1895 1595 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2863 2909 4117 104
Size: 2759 46 1208 1308
Fragments arranged by size:
2759 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2335 3113 3303 4095 2335
Size: 778 190 792 3561
Fragments arranged by size:
3561 792 778 190
BsmI GAATG_Cn'
Cuts at: 954 2230 954
Size: 1276 4045
Bsp1286I G_dGCh'C
Cuts at: 115 2335 2946 3039 3113 3303 3365 4095 115
Size: 2220 611 93 74 190 62 730 1341
Fragments arranged by size:
2220 1341 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4541 4541
Size: 5321
BspHI T'CATG_A
Cuts at: 4497 4497
Size: 5321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 2371 2585 2887 3268 1669
Size: 702 214 302 381 3722
Fragments arranged by size:
3722 702 381 302 214
BsrBI CCG'CTC
Cuts at: 50 858 3613 3667 5151 5316 50
Size: 808 2755 54 1484 165 55
Fragments arranged by size:
2755 1484 808 165 55 54
BsrDI GCAATG_nn'
Cuts at: 680 3233 680
Size: 2553 2768
BsrFI r'CCGG_y
Cuts at: 151 3319 3500 3709 151
Size: 3168 181 209 1763
Fragments arranged by size:
3168 1763 209 181
BsrGI T'GTAC_A
Cuts at: 1711 1711
Size: 5321
BssSI C'ACGA_G
Cuts at: 787 3592 3950 787
Size: 2805 358 2158
Fragments arranged by size:
2805 2158 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 2152 2698 1393
Size: 759 546 4016
Fragments arranged by size:
4016 759 546
BstBI TT'CG_AA
Cuts at: 3682 3682
Size: 5321
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 5321
BstXI CCAn_nnnn'nTGG
Cuts at: 1885 1885
Size: 5321
BstYI r'GATC_y
Cuts at: 1 729 932 1176 1414 1917 2346 2813 2836
Size: 728 203 244 238 503 429 467 23
Cuts at: 2836 3171 3417 4429 4515 4527 4873 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 728 503 467 449 429 346 335
246 244 238 203 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 905 2027 905
Size: 1122 4199
BtgI C'CryG_G
Cuts at: 107 107
Size: 5321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3271 3452 3615 3271
Size: 181 163 4977
Fragments arranged by size:
4977 181 163
BtsI GCAGTG_nn'
Cuts at: 320 2564 320
Size: 2244 3077
DraI TTT'AAA
Cuts at: 2320 2320
Size: 5321
DrdI GACnn_nn'nnGTC
Cuts at: 3027 3885 3027
Size: 858 4463
EaeI y'GGCC_r
Cuts at: 317 585 1092 2906 3080 3471 3498 4843 317
Size: 268 507 1814 174 391 27 1345 795
Fragments arranged by size:
1814 1345 795 507 391 268 174 27
EagI C'GGCC_G
Cuts at: 2906 2906
Size: 5321
EarI CTCTTCn'nnn_
Cuts at: 3344 3554 3344
Size: 210 5111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2093 3529 3839 3985 5131 2093
Size: 1436 310 146 1146 2283
Fragments arranged by size:
2283 1436 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2333 2333
Size: 5321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 746 766 1103 1526 1907 1925 1957 2269
Size: 198 20 337 423 381 18 32 312
Cuts at: 2269 3146 3578 4325 4593 548
Size: 877 432 747 268 1276
Fragments arranged by size:
1276 877 747 432 423 381 337 312
268 198 32 20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 432 2147 432
Size: 1715 3606
EcoO109I rG'GnC_Cy
Cuts at: 2140 2460 2140
Size: 320 5001
EcoRI G'AATT_C
Cuts at: 410 1856 2325 410
Size: 1446 469 3406
Fragments arranged by size:
3406 1446 469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2250 3340 2250
Size: 1090 4231
FspI TGC'GCA
Cuts at: 3102 3102
Size: 5321
HaeII r_GCGC'y
Cuts at: 2755 3003 4025 5004 2755
Size: 248 1022 979 3072
Fragments arranged by size:
3072 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1154 1186 1286 1318 1399 1431 1582 1614 1880
Size: 32 100 32 81 32 151 32 266
Cuts at: 1880 1912 2249 2281 3163 3193 3195 3225 1154
Size: 32 337 32 882 30 2 30 3250
Fragments arranged by size:
3250 882 337 266 151 100 81 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 399 2360 399
Size: 1961 3360
HindIII A'AGCT_T
Cuts at: 1969 2212 1969
Size: 243 5078
KasI G'GCGC_C
Cuts at: 2751 2999 2751
Size: 248 5073
KpnI G_GTAC'C
Cuts at: 2341 2341
Size: 5321
MfeI C'AATT_G
Cuts at: 644 644
Size: 5321
MluI A'CGCG_T
Cuts at: 4754 4754
Size: 5321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 804 3992 4176 804
Size: 3188 184 1949
Fragments arranged by size:
3188 1949 184
MscI TGG'CCA
Cuts at: 319 587 1094 3082 319
Size: 268 507 1988 2558
Fragments arranged by size:
2558 1988 507 268
MslI CAynn'nnrTG
Cuts at: 2197 3310 3437 2197
Size: 1113 127 4081
Fragments arranged by size:
4081 1113 127
MspA1I CmG'CkG
Cuts at: 120 2432 2746 3106 4119 4364 120
Size: 2312 314 360 1013 245 1077
Fragments arranged by size:
2312 1077 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3502 3502
Size: 5321
NarI GG'CG_CC
Cuts at: 2752 3000 2752
Size: 248 5073
NcoI C'CATG_G
Cuts at: 107 107
Size: 5321
NdeI CA'TA_TG
Cuts at: 1911 1911
Size: 5321
NgoMIV G'CCGG_C
Cuts at: 3500 3500
Size: 5321
NsiI A_TGCA'T
Cuts at: 1911 1911
Size: 5321
NspI r_CATG'y
Cuts at: 2374 2874 3781 2374
Size: 500 907 3914
Fragments arranged by size:
3914 907 500
PciI A'CATG_T
Cuts at: 3777 3777
Size: 5321
PflMI CCAn_nnn'nTGG
Cuts at: 1933 1933
Size: 5321
PfoI T'CCnGG_A
Cuts at: 1290 1812 1290
Size: 522 4799
PmeI GTTT'AAAC
Cuts at: 2320 2320
Size: 5321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 440 4460 4492 408
Size: 32 4020 32 1237
Fragments arranged by size:
4020 1237 32 32
PstI C_TGCA'G
Cuts at: 2368 3053 2368
Size: 685 4636
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5321
PvuII CAG'CTG
Cuts at: 2746 3106 2746
Size: 360 4961
RsrII CG'GwC_CG
Cuts at: 3516 3516
Size: 5321
SacI G_AGCT'C
Cuts at: 2335 2335
Size: 5321
SalI G'TCGA_C
Cuts at: 2358 2358
Size: 5321
SapI GCTCTTCn'nnn_
Cuts at: 3344 3554 3344
Size: 210 5111
SbfI CC_TGCA'GG
Cuts at: 2368 2368
Size: 5321
ScaI AGT'ACT
Cuts at: 1336 4838 1336
Size: 3502 1819
SfcI C'TryA_G
Cuts at: 33 1061 1550 2364 3049 4042 4233 33
Size: 1028 489 814 685 993 191 1121
Fragments arranged by size:
1121 1028 993 814 685 489 191
SfoI GGC'GCC
Cuts at: 2753 3001 2753
Size: 248 5073
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
SgrAI Cr'CCGG_yG
Cuts at: 151 151
Size: 5321
SmaI CCC'GGG
Cuts at: 2343 2343
Size: 5321
SmlI C'TyrA_G
Cuts at: 2476 3391 3883 4145 2476
Size: 915 492 262 3652
Fragments arranged by size:
3652 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1045 67
Size: 978 4343
SphI G_CATG'C
Cuts at: 2374 2874 2374
Size: 500 4821
SspI AAT'ATT
Cuts at: 2085 2085
Size: 5321
StyI C'CwwG_G
Cuts at: 107 1152 1871 2136 2455 2809 107
Size: 1045 719 265 319 354 2619
Fragments arranged by size:
2619 1045 719 354 319 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 908 1258 2904 3037 3496 3705 908
Size: 350 1646 133 459 209 2524
Fragments arranged by size:
2524 1646 459 350 209 133
TatI w'GTAC_w
Cuts at: 1334 1581 1711 4836 1334
Size: 247 130 3125 1819
Fragments arranged by size:
3125 1819 247 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 669 701 755 787 4838 4870 299
Size: 32 338 32 54 32 4051 32 750
Fragments arranged by size:
4051 750 338 54 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1537 1894 3118 1537
Size: 357 1224 3740
Fragments arranged by size:
3740 1224 357
XbaI T'CTAG_A
Cuts at: 2352 2352
Size: 5321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 979 2816 979
Size: 1837 3484
XmaI C'CCGG_G
Cuts at: 2341 2341
Size: 5321
XmnI GAAnn'nnTTC
Cuts at: 2230 2230
Size: 5321
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NheI NotI NruI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI StuI SwaI
XhoI ZraI