Restriction map for
pF1KE2340
(Circular) (Six-base) MAPSORT of: pF1KE2340.seq Check: 3343 from: 1 to: 3623
pF1KE2340 3623 bp
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AatII G_ACGT'C
Cuts at: 404 404
Size: 3623
AccI GT'mk_AC
Cuts at: 529 661 529
Size: 132 3491
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 578 1448 1880 2627 2895 165
Size: 413 870 432 747 268 893
Fragments arranged by size:
893 870 747 432 413 268
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 450 450
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 928 2495 928
Size: 1567 2056
ApaLI G'TGCA_C
Cuts at: 2393 2393
Size: 3623
ApoI r'AATT_y
Cuts at: 627 2976 3232 627
Size: 2349 256 1018
Fragments arranged by size:
2349 1018 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 199 643 199
Size: 444 3179
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 639 1053 1301 1336 639
Size: 414 248 35 2926
Fragments arranged by size:
2926 414 248 35
BanII G_rGCy'C
Cuts at: 194 637 1667 194
Size: 443 1030 2150
Fragments arranged by size:
2150 1030 443
BbeI G_GCGC'C
Cuts at: 1057 1305 1057
Size: 248 3375
BbsI GAAGACnn'nnnn_
Cuts at: 566 566
Size: 3623
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglI GCCn_nnn'nGGC
Cuts at: 353 505 353
Size: 152 3471
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 735 735
Size: 3623
Bme1580I G_kGCm'C
Cuts at: 1248 1341 2397 1248
Size: 93 1056 2474
Fragments arranged by size:
2474 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 255 835 916 1251 3395 255
Size: 580 81 335 2144 483
Fragments arranged by size:
2144 580 483 335 81
BmtI G_CTAG'C
Cuts at: 457 457
Size: 3623
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 3623
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 401 1054 1302 2004 3500 401
Size: 653 248 702 1496 524
Fragments arranged by size:
1496 702 653 524 248
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2285 2432 2843 812
Size: 521 678 274 147 411 1592
Fragments arranged by size:
1592 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 147 177 147
Size: 30 3593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 403 265
Size: 138 3485
BseYI C'CCAG_C
Cuts at: 495 1048 1806 2383 2879 495
Size: 553 758 577 496 1239
Fragments arranged by size:
1239 758 577 553 496
BsiEI CG_ry'CG
Cuts at: 104 171 1165 1211 2419 104
Size: 67 994 46 1208 1308
Fragments arranged by size:
1308 1208 994 67 46
BsiHKAI G_wGCw'C
Cuts at: 464 637 1415 1605 2397 464
Size: 173 778 190 792 1690
Fragments arranged by size:
1690 792 778 190 173
Bsp1286I G_dGCh'C
Cuts at: 194 464 637 1248 1341 1415 1605 1667 2397
Size: 270 173 611 93 74 190 62 730
Cuts at: 2397 194
Size: 1420
Fragments arranged by size:
1420 730 611 270 190 173 93 74
62
BspEI T'CCGG_A
Cuts at: 2843 2843
Size: 3623
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 673 887 1189 1570 673
Size: 214 302 381 2726
Fragments arranged by size:
2726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1915 1969 3453 3618 50
Size: 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 1535
Size: 3623
BsrFI r'CCGG_y
Cuts at: 118 170 482 1621 1802 2011 118
Size: 52 312 1139 181 209 1730
Fragments arranged by size:
1730 1139 312 209 181 52
BssSI C'ACGA_G
Cuts at: 1894 2252 1894
Size: 358 3265
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3623
BstYI r'GATC_y
Cuts at: 1 444 648 1115 1138 1473 1719 2731 2817
Size: 443 204 467 23 335 246 1012 86
Cuts at: 2817 2829 3175 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 443 346 335 246 204
86 23 12
BstZ17I GTA'TAC
Cuts at: 530 530
Size: 3623
BtgI C'CryG_G
Cuts at: 543 543
Size: 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 155 464 866 155
Size: 309 402 2912
Fragments arranged by size:
2912 402 309
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 116 168 1208 1382 1773 1800 3145 116
Size: 52 1040 174 391 27 1345 594
Fragments arranged by size:
1345 1040 594 391 174 52 27
EagI C'GGCC_G
Cuts at: 168 1208 168
Size: 1040 2583
EarI CTCTTCn'nnn_
Cuts at: 522 1646 1856 522
Size: 1124 210 2289
Fragments arranged by size:
2289 1124 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 1831 2141 2287 3433 199
Size: 1632 310 146 1146 389
Fragments arranged by size:
1632 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 635 635
Size: 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 578 1448 1880 2627 2895 165
Size: 413 870 432 747 268 893
Fragments arranged by size:
893 870 747 432 413 268
EcoNI CCTnn'n_nnAGG
Cuts at: 385 385
Size: 3623
EcoO109I rG'GnC_Cy
Cuts at: 574 762 574
Size: 188 3435
EcoRI G'AATT_C
Cuts at: 627 627
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 409 1642 409
Size: 1233 2390
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 1057 1305 2327 3306 1057
Size: 248 1022 979 1374
Fragments arranged by size:
1374 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1465 1495 1497 1527 1465
Size: 30 2 30 3561
Fragments arranged by size:
3561 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
KasI G'GCGC_C
Cuts at: 1053 1301 1053
Size: 248 3375
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 1384 1384
Size: 3623
MslI CAynn'nnrTG
Cuts at: 112 1612 1739 112
Size: 1500 127 1996
Fragments arranged by size:
1996 1500 127
MspA1I CmG'CkG
Cuts at: 434 734 1048 1408 2421 2666 434
Size: 300 314 360 1013 245 1391
Fragments arranged by size:
1391 1013 360 314 300 245
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 1054 1302 1054
Size: 248 3375
NcoI C'CATG_G
Cuts at: 543 543
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NheI G'CTAG_C
Cuts at: 453 453
Size: 3623
NspI r_CATG'y
Cuts at: 676 1176 2083 676
Size: 500 907 2216
Fragments arranged by size:
2216 907 500
PasI CC'CwG_GG
Cuts at: 365 365
Size: 3623
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PflMI CCAn_nnn'nTGG
Cuts at: 570 570
Size: 3623
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 3591
PspXI vC'TCGA_Gb
Cuts at: 199 199
Size: 3623
PstI C_TGCA'G
Cuts at: 670 1355 670
Size: 685 2938
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 1048 1408 1048
Size: 360 3263
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 637 637
Size: 3623
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 522 1646 1856 522
Size: 1124 210 2289
Fragments arranged by size:
2289 1124 210
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SexAI A'CCwGG_T
Cuts at: 413 413
Size: 3623
SfcI C'TryA_G
Cuts at: 33 666 1351 2344 2535 33
Size: 633 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 191
SfoI GGC'GCC
Cuts at: 1055 1303 1055
Size: 248 3375
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 199 778 1693 2185 2447 199
Size: 579 915 492 262 1375
Fragments arranged by size:
1375 915 579 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 284 351 543 757 1111 284
Size: 67 192 214 354 2796
Fragments arranged by size:
2796 354 214 192 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 3138 3138
Size: 3623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 3140 3172 147
Size: 32 2961 32 598
Fragments arranged by size:
2961 598 32 32
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1118 1118
Size: 3623
XhoI C'TCGA_G
Cuts at: 199 199
Size: 3623
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
XmnI GAAnn'nnTTC
Cuts at: 317 317
Size: 3623
ZraI GAC'GTC
Cuts at: 402 402
Size: 3623
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BpmI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI