Restriction map for
pF1KE2336
(Circular) (Six-base) MAPSORT of: pF1KE2336.seq Check: 7543 from: 1 to: 7088
pF1KE2336 7088 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 3980 4126 3980
Size: 146 6942
Acc65I G'GTAC_C
Cuts at: 4104 4104
Size: 7088
AclI AA'CG_TT
Cuts at: 6928 6928
Size: 7088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2205 3131 3301 4913 5345 6092 6360 2204
Size: 1 926 170 1612 432 747 268 2932
Fragments arranged by size:
2932 1612 926 747 432 268 170 1
AflIII A'CryG_T
Cuts at: 168 4440 5544 6521 168
Size: 4272 1104 977 735
Fragments arranged by size:
4272 1104 977 735
AgeI A'CCGG_T
Cuts at: 5476 5476
Size: 7088
AleI CACnn'nnGTG
Cuts at: 1123 1123
Size: 7088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 791 791
Size: 7088
AlwNI CAG_nnn'CTG
Cuts at: 784 1917 2883 4393 5960 784
Size: 1133 966 1510 1567 1912
Fragments arranged by size:
1912 1567 1510 1133 966
ApaLI G'TGCA_C
Cuts at: 5858 5858
Size: 7088
ApoI r'AATT_y
Cuts at: 395 1065 1847 2405 2567 3398 3772 4092 6441
Size: 670 782 558 162 831 374 320 2349
Cuts at: 6441 6697 395
Size: 256 786
Fragments arranged by size:
2349 831 786 782 670 558 374 320
256 162
AseI AT'TA_AT
Cuts at: 20 458 20
Size: 438 6650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7088
AvaI C'yCGr_G
Cuts at: 1483 1563 4108 1483
Size: 80 2545 4463
Fragments arranged by size:
4463 2545 80
BamHI G'GATC_C
Cuts at: 3513 3628 3729 4113 3513
Size: 115 101 384 6488
Fragments arranged by size:
6488 384 115 101
BanI G'GyrC_C
Cuts at: 4104 4518 4766 4801 4104
Size: 414 248 35 6391
Fragments arranged by size:
6391 414 248 35
BanII G_rGCy'C
Cuts at: 2840 4102 5132 2840
Size: 1262 1030 4796
Fragments arranged by size:
4796 1262 1030
BbeI G_GCGC'C
Cuts at: 4522 4770 4522
Size: 248 6840
BbsI GAAGACnn'nnnn_
Cuts at: 263 676 2069 2238 3864 263
Size: 413 1393 169 1626 3487
Fragments arranged by size:
3487 1626 1393 413 169
BbvCI CC'TCA_GC
Cuts at: 1218 1218
Size: 7088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1080 1114 90
Size: 34 956 34 6064
Fragments arranged by size:
6064 956 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3600 4986 5747 7088 3600
Size: 1386 761 1341 3600
Fragments arranged by size:
3600 1386 1341 761
BclI T'GATC_A
Cuts at: 723 1142 4608 723
Size: 419 3466 3203
Fragments arranged by size:
3466 3203 419
BglII A'GATC_T
Cuts at: 2385 2452 3413 3588 4603 6640 2385
Size: 67 961 175 1015 2037 2833
Fragments arranged by size:
2833 2037 1015 961 175 67
BlpI GC'TnA_GC
Cuts at: 4200 4200
Size: 7088
Bme1580I G_kGCm'C
Cuts at: 4713 4806 5862 4713
Size: 93 1056 5939
Fragments arranged by size:
5939 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2149 2328 3943 4300 4381 4716 6860 2149
Size: 179 1615 357 81 335 2144 2377
Fragments arranged by size:
2377 2144 1615 357 335 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5455 5455
Size: 7088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2149 2815 2015
Size: 134 666 6288
Fragments arranged by size:
6288 666 134
Bpu10I CC'TnA_GC
Cuts at: 1218 2279 1218
Size: 1061 6027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5143 5635 5933 4264
Size: 879 492 298 5419
Fragments arranged by size:
5419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1372 2093 2673 6792 1372
Size: 721 580 4119 1668
Fragments arranged by size:
4119 1668 721 580
BsaAI yAC'GTr
Cuts at: 1125 4441 1125
Size: 3316 3772
BsaBI GATnn'nnATC
Cuts at: 6 1676 2552 4602 6639 6
Size: 1670 876 2050 2037 455
Fragments arranged by size:
2050 2037 1670 876 455
BsaHI Gr'CG_yC
Cuts at: 4519 4767 5469 6965 4519
Size: 248 702 1496 4642
Fragments arranged by size:
4642 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2532 3337 4277 4798 5476 5750 5897 6308 2532
Size: 805 940 521 678 274 147 411 3312
Fragments arranged by size:
3312 940 805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2354 2384 2354
Size: 30 7058
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 906 1120 1590 2185 2375 295
Size: 611 214 470 595 190 5008
Fragments arranged by size:
5008 611 595 470 214 190
BseYI C'CCAG_C
Cuts at: 592 823 4513 5271 5848 6344 592
Size: 231 3690 758 577 496 1336
Fragments arranged by size:
3690 1336 758 577 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 1817 1963 3768 1363
Size: 454 146 1805 4683
Fragments arranged by size:
4683 1805 454 146
BsiEI CG_ry'CG
Cuts at: 104 4630 4676 5884 104
Size: 4526 46 1208 1308
Fragments arranged by size:
4526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1130 1484 1644 2127 2235 3530 4102 4880 5070
Size: 354 160 483 108 1295 572 778 190
Cuts at: 5070 5862 1130
Size: 792 2356
Fragments arranged by size:
2356 1295 792 778 572 483 354 190
160 108
BsiWI C'GTAC_G
Cuts at: 509 509
Size: 7088
BsmI GAATG_Cn'
Cuts at: 3555 3555
Size: 7088
Bsp1286I G_dGCh'C
Cuts at: 1130 1484 1644 2127 2235 2840 3530 4102 4713
Size: 354 160 483 108 605 690 572 611
Cuts at: 4713 4806 4880 5070 5132 5862 1130
Size: 93 74 190 62 730 2356
Fragments arranged by size:
2356 730 690 611 605 572 483 354
190 160 108 93 74 62
BspEI T'CCGG_A
Cuts at: 6308 6308
Size: 7088
BspHI T'CATG_A
Cuts at: 6264 6264
Size: 7088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3030 4138 4352 4654 5035 3030
Size: 1108 214 302 381 5083
Fragments arranged by size:
5083 1108 381 302 214
BsrBI CCG'CTC
Cuts at: 50 263 5380 5434 6918 7083 50
Size: 213 5117 54 1484 165 55
Fragments arranged by size:
5117 1484 213 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2644 3797 5000 2644
Size: 1153 1203 4732
Fragments arranged by size:
4732 1203 1153
BsrFI r'CCGG_y
Cuts at: 5086 5267 5476 5086
Size: 181 209 6698
Fragments arranged by size:
6698 209 181
BsrGI T'GTAC_A
Cuts at: 3254 3254
Size: 7088
BssSI C'ACGA_G
Cuts at: 5359 5717 5359
Size: 358 6730
BstAPI GCAn_nnn'nTGC
Cuts at: 784 1804 4465 784
Size: 1020 2661 3407
Fragments arranged by size:
3407 2661 1020
BstBI TT'CG_AA
Cuts at: 1829 5449 1829
Size: 3620 3468
BstEII G'GTnAC_C
Cuts at: 1200 1200
Size: 7088
BstXI CCAn_nnnn'nTGG
Cuts at: 444 3209 444
Size: 2765 4323
BstYI r'GATC_y
Cuts at: 1 2385 2452 3413 3513 3588 3628 3729 4113
Size: 2384 67 961 100 75 40 101 384
Cuts at: 4113 4580 4603 4938 5184 6196 6282 6294 6640
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6640 1
Size: 449
Fragments arranged by size:
2384 1012 961 467 449 384 346 335
246 101 100 86 75 67 40 23
12
BstZ17I GTA'TAC
Cuts at: 3981 3981
Size: 7088
Bsu36I CC'TnA_GG
Cuts at: 1054 3470 1054
Size: 2416 4672
BtgI C'CryG_G
Cuts at: 482 521 662 1445 482
Size: 39 141 783 6125
Fragments arranged by size:
6125 783 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5038 5219 5382 5038
Size: 181 163 6744
Fragments arranged by size:
6744 181 163
BtsI GCAGTG_nn'
Cuts at: 763 785 1143 1175 1757 2270 3780 4331 763
Size: 22 358 32 582 513 1510 551 3520
Fragments arranged by size:
3520 1510 582 551 513 358 32 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2287 2322 2287
Size: 35 7053
DraI TTT'AAA
Cuts at: 201 2000 2573 4087 201
Size: 1799 573 1514 3202
Fragments arranged by size:
3202 1799 1514 573
DrdI GACnn_nn'nnGTC
Cuts at: 4794 5652 4794
Size: 858 6230
EaeI y'GGCC_r
Cuts at: 207 659 2681 4673 4847 5238 5265 6610 207
Size: 452 2022 1992 174 391 27 1345 685
Fragments arranged by size:
2022 1992 1345 685 452 391 174 27
EagI C'GGCC_G
Cuts at: 4673 4673
Size: 7088
EarI CTCTTCn'nnn_
Cuts at: 1213 2490 2861 5111 5321 1213
Size: 1277 371 2250 210 2980
Fragments arranged by size:
2980 2250 1277 371 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5296 5606 5752 6898 5296
Size: 310 146 1146 5486
Fragments arranged by size:
5486 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4100 4100
Size: 7088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2149 2204 2205 2815 3131 3301 4913 5345
Size: 134 55 1 610 316 170 1612 432
Cuts at: 5345 6092 6360 2015
Size: 747 268 2743
Fragments arranged by size:
2743 1612 747 610 432 316 268 170
134 55 1
EcoNI CCTnn'n_nnAGG
Cuts at: 277 1053 277
Size: 776 6312
EcoO109I rG'GnC_Cy
Cuts at: 3005 4227 3005
Size: 1222 5866
EcoRI G'AATT_C
Cuts at: 4092 4092
Size: 7088
EcoRV GAT'ATC
Cuts at: 1292 1292
Size: 7088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2235 5107 2235
Size: 2872 4216
FspI TGC'GCA
Cuts at: 4869 4869
Size: 7088
HaeII r_GCGC'y
Cuts at: 2781 4522 4770 5792 6771 2781
Size: 1741 248 1022 979 3098
Fragments arranged by size:
3098 1741 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 678 710 1219 1251 1276 1308 1357 1371 1389
Size: 32 509 32 25 32 49 14 18
Cuts at: 1389 1403 1680 1712 1975 2007 3215 3247 4930
Size: 14 277 32 263 32 1208 32 1683
Cuts at: 4930 4960 4962 4992 678
Size: 30 2 30 2774
Fragments arranged by size:
2774 1683 1208 509 277 263 49 32
32 32 32 32 32 30 30 25
18 14 14 2
HincII GTy'rAC
Cuts at: 2481 4127 2481
Size: 1646 5442
HpaI GTT'AAC
Cuts at: 2481 2481
Size: 7088
KasI G'GCGC_C
Cuts at: 4518 4766 4518
Size: 248 6840
KpnI G_GTAC'C
Cuts at: 4108 4108
Size: 7088
MluI A'CGCG_T
Cuts at: 168 6521 168
Size: 6353 735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1646 3624 5759 5943 1646
Size: 1978 2135 184 2791
Fragments arranged by size:
2791 2135 1978 184
MscI TGG'CCA
Cuts at: 209 661 4849 209
Size: 452 4188 2448
Fragments arranged by size:
4188 2448 452
MslI CAynn'nnrTG
Cuts at: 661 1123 2603 3073 3079 3533 5077 5204 661
Size: 462 1480 470 6 454 1544 127 2545
Fragments arranged by size:
2545 1544 1480 470 462 454 127 6
MspA1I CmG'CkG
Cuts at: 144 1311 1662 2619 2945 3952 4199 4513 4873
Size: 1167 351 957 326 1007 247 314 360
Cuts at: 4873 5886 6131 144
Size: 1013 245 1101
Fragments arranged by size:
1167 1101 1013 1007 957 360 351 326
314 247 245
NaeI GCC'GGC
Cuts at: 5269 5269
Size: 7088
NarI GG'CG_CC
Cuts at: 4519 4767 4519
Size: 248 6840
NcoI C'CATG_G
Cuts at: 482 662 482
Size: 180 6908
NdeI CA'TA_TG
Cuts at: 623 623
Size: 7088
NgoMIV G'CCGG_C
Cuts at: 5267 5267
Size: 7088
NspI r_CATG'y
Cuts at: 1342 4141 4641 5548 1342
Size: 2799 500 907 2882
Fragments arranged by size:
2882 2799 907 500
PasI CC'CwG_GG
Cuts at: 1553 1553
Size: 7088
PciI A'CATG_T
Cuts at: 5544 5544
Size: 7088
PflMI CCAn_nnn'nTGG
Cuts at: 4006 4006
Size: 7088
PfoI T'CCnGG_A
Cuts at: 1778 2037 3764 1778
Size: 259 1727 5102
Fragments arranged by size:
5102 1727 259
PmeI GTTT'AAAC
Cuts at: 4087 4087
Size: 7088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 148 180 2905 2937 6227 6259 148
Size: 32 2725 32 3290 32 977
Fragments arranged by size:
3290 2725 977 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3005 3005
Size: 7088
PsiI TTA'TAA
Cuts at: 946 946
Size: 7088
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 493 525 2417 2449 3238 3270 493
Size: 32 1892 32 789 32 4311
Fragments arranged by size:
4311 1892 789 32 32 32
PstI C_TGCA'G
Cuts at: 149 770 2277 4135 4820 149
Size: 621 1507 1858 685 2417
Fragments arranged by size:
2417 1858 1507 685 621
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7088
PvuII CAG'CTG
Cuts at: 1311 1662 4513 4873 1311
Size: 351 2851 360 3526
Fragments arranged by size:
3526 2851 360 351
RsrII CG'GwC_CG
Cuts at: 5283 5283
Size: 7088
SacI G_AGCT'C
Cuts at: 4102 4102
Size: 7088
SalI G'TCGA_C
Cuts at: 4125 4125
Size: 7088
SanDI GG'GwC_CC
Cuts at: 3005 3005
Size: 7088
SapI GCTCTTCn'nnn_
Cuts at: 5111 5321 5111
Size: 210 6878
SbfI CC_TGCA'GG
Cuts at: 4135 4135
Size: 7088
ScaI AGT'ACT
Cuts at: 6605 6605
Size: 7088
SfcI C'TryA_G
Cuts at: 33 145 766 2273 2423 4131 4816 5809 6000
Size: 112 621 1507 150 1708 685 993 191
Cuts at: 6000 33
Size: 1121
Fragments arranged by size:
1708 1507 1121 993 685 621 191 150
112
SfoI GGC'GCC
Cuts at: 4520 4768 4520
Size: 248 6840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7088
SmaI CCC'GGG
Cuts at: 4110 4110
Size: 7088
SmlI C'TyrA_G
Cuts at: 4243 5158 5650 5912 4243
Size: 915 492 262 5419
Fragments arranged by size:
5419 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7088
SphI G_CATG'C
Cuts at: 4141 4641 4141
Size: 500 6588
SspI AAT'ATT
Cuts at: 1584 1584
Size: 7088
StuI AGG'CCT
Cuts at: 1397 2206 1397
Size: 809 6279
StyI C'CwwG_G
Cuts at: 273 482 662 2692 2708 2718 3178 4222 4576
Size: 209 180 2030 16 10 460 1044 354
Cuts at: 4576 273
Size: 2785
Fragments arranged by size:
2785 2030 1044 460 354 209 180 16
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1545 4671 4804 5263 5472 1545
Size: 3126 133 459 209 3161
Fragments arranged by size:
3161 3126 459 209 133
TatI w'GTAC_w
Cuts at: 3254 6603 3254
Size: 3349 3739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6605 6637 6605
Size: 32 7056
Tth111I GACn'n_nGTC
Cuts at: 2937 4885 2937
Size: 1948 5140
XbaI T'CTAG_A
Cuts at: 2101 4119 2101
Size: 2018 5070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 3206 4583 489
Size: 2717 1377 2994
Fragments arranged by size:
2994 2717 1377
XmaI C'CCGG_G
Cuts at: 4108 4108
Size: 7088
XmnI GAAnn'nnTTC
Cuts at: 877 2218 2409 3351 3590 877
Size: 1341 191 942 239 4375
Fragments arranged by size:
4375 1341 942 239 191
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsmBI BssHII
ClaI DraIII FseI FspAI HindIII MfeI NheI NotI
NruI NsiI PacI PmlI PshAI PspOMI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI