Restriction map for
pF1KE2333
(Circular) (Six-base) MAPSORT of: pF1KE2333.seq Check: 2815 from: 1 to: 4538
pF1KE2333 4538 bp
With 182 enzymes: SgfI *
April 25, 2011 13:19 ..
AatII G_ACGT'C
Cuts at: 1156 1156
Size: 4538
AccI GT'mk_AC
Cuts at: 1576 1576
Size: 4538
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1263 2363 2795 3542 3810 1263
Size: 1100 432 747 268 1991
Fragments arranged by size:
1991 1100 747 432 268
AflIII A'CryG_T
Cuts at: 1352 1890 2994 3971 1352
Size: 538 1104 977 1919
Fragments arranged by size:
1919 1104 977 538
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AleI CACnn'nnGTG
Cuts at: 524 524
Size: 4538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1388 1388
Size: 4538
AlwNI CAG_nnn'CTG
Cuts at: 487 1843 3410 487
Size: 1356 1567 1615
Fragments arranged by size:
1615 1567 1356
ApaI G_GGCC'C
Cuts at: 125 125
Size: 4538
ApaLI G'TGCA_C
Cuts at: 792 3308 792
Size: 2516 2022
ApoI r'AATT_y
Cuts at: 1191 1542 3891 4147 1191
Size: 351 2349 256 1582
Fragments arranged by size:
2349 1582 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 1087 1262 1493 1558 1087
Size: 175 231 65 4067
Fragments arranged by size:
4067 231 175 65
BamHI G'GATC_C
Cuts at: 1024 1563 1024
Size: 539 3999
BanI G'GyrC_C
Cuts at: 510 1554 1968 2216 2251 510
Size: 1044 414 248 35 2797
Fragments arranged by size:
2797 1044 414 248 35
BanII G_rGCy'C
Cuts at: 125 681 1269 1552 2582 125
Size: 556 588 283 1030 2081
Fragments arranged by size:
2081 1030 588 556 283
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 384 418 384
Size: 34 4504
BciVI GTATCCnnnnn_n'
Cuts at: 625 1427 2436 3197 4538 625
Size: 802 1009 761 1341 625
Fragments arranged by size:
1341 1009 802 761 625
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglI GCCn_nnn'nGGC
Cuts at: 289 581 289
Size: 292 4246
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1650 1650
Size: 4538
Bme1580I G_kGCm'C
Cuts at: 125 440 796 2163 2256 3312 125
Size: 315 356 1367 93 1056 1351
Fragments arranged by size:
1367 1351 1056 356 315 93
BmrI ACTGGGnnnn_n'
Cuts at: 209 443 1230 1750 1831 2166 4310 209
Size: 234 787 520 81 335 2144 437
Fragments arranged by size:
2144 787 520 437 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1288 2905 1288
Size: 1617 2921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 563 623 813 371
Size: 192 60 190 4096
Fragments arranged by size:
4096 192 190 60
Bpu10I CC'TnA_GC
Cuts at: 247 816 247
Size: 569 3969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 1714 2593 3085 3383 551
Size: 1163 879 492 298 1706
Fragments arranged by size:
1706 1163 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 2052 4089 6
Size: 2046 2037 455
Fragments arranged by size:
2046 2037 455
BsaHI Gr'CG_yC
Cuts at: 242 1153 1969 2217 2919 4415 242
Size: 911 816 248 702 1496 365
Fragments arranged by size:
1496 911 816 702 365 248
BsaWI w'CCGG_w
Cuts at: 1727 2248 2926 3200 3347 3758 1727
Size: 521 678 274 147 411 2507
Fragments arranged by size:
2507 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1029 1032 1261 1029
Size: 3 229 4306
Fragments arranged by size:
4306 229 3
BseYI C'CCAG_C
Cuts at: 746 1206 1255 1963 2721 3298 3794 746
Size: 460 49 708 758 577 496 1490
Fragments arranged by size:
1490 758 708 577 496 460 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1394
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 681 796 1552 2330 2520 3312 681
Size: 115 756 778 190 792 1907
Fragments arranged by size:
1907 792 778 756 190 115
BsmBI CGTCTCn'nnnn_
Cuts at: 233 1144 1160 233
Size: 911 16 3611
Fragments arranged by size:
3611 911 16
Bsp1286I G_dGCh'C
Cuts at: 125 440 681 796 1269 1552 2163 2256 2330
Size: 315 241 115 473 283 611 93 74
Cuts at: 2330 2520 2582 3312 125
Size: 190 62 730 1351
Fragments arranged by size:
1351 730 611 473 315 283 241 190
115 93 74 62
BspEI T'CCGG_A
Cuts at: 3758 3758
Size: 4538
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 351 1588 1802 2104 2485 351
Size: 1237 214 302 381 2404
Fragments arranged by size:
2404 1237 381 302 214
BsrBI CCG'CTC
Cuts at: 50 185 1064 1124 2830 2884 4368 4533 50
Size: 135 879 60 1706 54 1484 165 55
Fragments arranged by size:
1706 1484 879 165 135 60 55 54
BsrDI GCAATG_nn'
Cuts at: 705 891 2450 705
Size: 186 1559 2793
Fragments arranged by size:
2793 1559 186
BsrFI r'CCGG_y
Cuts at: 1033 2536 2717 2926 1033
Size: 1503 181 209 2645
Fragments arranged by size:
2645 1503 209 181
BssSI C'ACGA_G
Cuts at: 439 2809 3167 439
Size: 2370 358 1810
Fragments arranged by size:
2370 1810 358
BstAPI GCAn_nnn'nTGC
Cuts at: 418 510 1915 418
Size: 92 1405 3041
Fragments arranged by size:
3041 1405 92
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstYI r'GATC_y
Cuts at: 1 1024 1563 2030 2053 2388 2634 3646 3732
Size: 1023 539 467 23 335 246 1012 86
Cuts at: 3732 3744 4090 1
Size: 12 346 449
Fragments arranged by size:
1023 1012 539 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 671 671
Size: 4538
BtgI C'CryG_G
Cuts at: 492 549 619 627 1053 1116 492
Size: 57 70 8 426 63 3914
Fragments arranged by size:
3914 426 70 63 57 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 165 876 882 918 930 942 954 966 978
Size: 711 6 36 12 12 12 12 12
Cuts at: 978 1014 1360 1421 1522 1781 165
Size: 36 346 61 101 259 2922
Fragments arranged by size:
2922 711 346 259 101 61 36 36
12 12 12 12 12 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 966 1001 1038 1073 966
Size: 35 37 35 4431
Fragments arranged by size:
4431 37 35 35
DraI TTT'AAA
Cuts at: 1197 1537 1197
Size: 340 4198
DrdI GACnn_nn'nnGTC
Cuts at: 2244 3102 2244
Size: 858 3680
EaeI y'GGCC_r
Cuts at: 321 516 2123 2297 2688 2715 4060 321
Size: 195 1607 174 391 27 1345 799
Fragments arranged by size:
1607 1345 799 391 195 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1082 2746 3056 3202 4348 1082
Size: 1664 310 146 1146 1272
Fragments arranged by size:
1664 1272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 679 1550 679
Size: 871 3667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 563 623 813 1263 2363 2795 3542 3810
Size: 192 60 190 450 1100 432 747 268
Cuts at: 3810 371
Size: 1099
Fragments arranged by size:
1100 1099 747 450 432 268 192 190
60
EcoO109I rG'GnC_Cy
Cuts at: 121 122 219 315 1245 1677 121
Size: 1 97 96 930 432 2982
Fragments arranged by size:
2982 930 432 97 96 1
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 592 2557 592
Size: 1965 2573
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 1972 2220 3242 4221 1972
Size: 248 1022 979 2289
Fragments arranged by size:
2289 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 714 746 1342 1374 2380 2410 2412 2442 714
Size: 32 596 32 1006 30 2 30 2810
Fragments arranged by size:
2810 1006 596 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1577 1577
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 1352 3971 1352
Size: 2619 1919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3393 3209
Size: 184 4354
MscI TGG'CCA
Cuts at: 323 518 2299 323
Size: 195 1781 2562
Fragments arranged by size:
2562 1781 195
MslI CAynn'nnrTG
Cuts at: 524 2527 2654 524
Size: 2003 127 2408
Fragments arranged by size:
2408 2003 127
MspA1I CmG'CkG
Cuts at: 295 494 851 1004 1118 1649 1963 2323 3336
Size: 199 357 153 114 531 314 360 1013
Cuts at: 3336 3581 295
Size: 245 1252
Fragments arranged by size:
1252 1013 531 360 357 314 245 199
153 114
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 549 549
Size: 4538
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 1414 1591 2091 2998 1414
Size: 177 500 907 2954
Fragments arranged by size:
2954 907 500 177
PasI CC'CwG_GG
Cuts at: 683 683
Size: 4538
PciI A'CATG_T
Cuts at: 2994 2994
Size: 4538
PflMI CCAn_nnn'nTGG
Cuts at: 373 423 718 811 373
Size: 50 295 93 4100
Fragments arranged by size:
4100 295 93 50
PfoI T'CCnGG_A
Cuts at: 600 600
Size: 4538
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PpuMI rG'GwC_Cy
Cuts at: 1245 1245
Size: 4538
PspOMI G'GGCC_C
Cuts at: 121 121
Size: 4538
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1384 1416 1384
Size: 32 4506
PstI C_TGCA'G
Cuts at: 182 1367 1517 1585 2270 182
Size: 1185 150 68 685 2450
Fragments arranged by size:
2450 1185 685 150 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 295 1963 2323 295
Size: 1668 360 2510
Fragments arranged by size:
2510 1668 360
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 681 1552 681
Size: 871 3667
SacII CC_GC'GG
Cuts at: 495 1119 495
Size: 624 3914
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 1441 4055 1441
Size: 2614 1924
SexAI A'CCwGG_T
Cuts at: 1241 1241
Size: 4538
SfcI C'TryA_G
Cuts at: 33 178 1363 1513 1581 2266 3259 3450 33
Size: 145 1185 150 68 685 993 191 1121
Fragments arranged by size:
1185 1121 993 685 191 150 145 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 581 581
Size: 4538
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1089 1495 1560 1089
Size: 406 65 4067
Fragments arranged by size:
4067 406 65
SmlI C'TyrA_G
Cuts at: 566 1693 2608 3100 3362 566
Size: 1127 915 492 262 1742
Fragments arranged by size:
1742 1127 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1414 1591 2091 1414
Size: 177 500 3861
Fragments arranged by size:
3861 500 177
SspI AAT'ATT
Cuts at: 210 210
Size: 4538
StyI C'CwwG_G
Cuts at: 222 318 324 549 576 1672 2026 222
Size: 96 6 225 27 1096 354 2734
Fragments arranged by size:
2734 1096 354 225 96 27 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 2121 2254 2713 2922 1509
Size: 612 133 459 209 3125
Fragments arranged by size:
3125 612 459 209 133
TatI w'GTAC_w
Cuts at: 1439 4053 1439
Size: 2614 1924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4506
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 556 598 754 2033 556
Size: 42 156 1279 3061
Fragments arranged by size:
3061 1279 156 42
XmaI C'CCGG_G
Cuts at: 1087 1493 1558 1087
Size: 406 65 4067
Fragments arranged by size:
4067 406 65
ZraI GAC'GTC
Cuts at: 1154 1154
Size: 4538
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
SanDI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI