Restriction map for
pF1KE2317
(Circular) (Six-base) MAPSORT of: pF1KE2317.seq Check: 6858 from: 1 to: 6266
pF1KE2317 6266 bp
With 182 enzymes: SgfI *
January 12, 2011 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 984 1002 984
Size: 18 6248
AatII G_ACGT'C
Cuts at: 2051 2051
Size: 6266
AccI GT'mk_AC
Cuts at: 3304 3304
Size: 6266
Acc65I G'GTAC_C
Cuts at: 3282 3282
Size: 6266
AclI AA'CG_TT
Cuts at: 6106 6106
Size: 6266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 4091 4523 5270 5538 2287
Size: 1804 432 747 268 3015
Fragments arranged by size:
3015 1804 747 432 268
AfeI AGC'GCT
Cuts at: 307 2101 307
Size: 1794 4472
AflIII A'CryG_T
Cuts at: 470 3618 4722 5699 470
Size: 3148 1104 977 1037
Fragments arranged by size:
3148 1104 1037 977
AgeI A'CCGG_T
Cuts at: 4654 4654
Size: 6266
AleI CACnn'nnGTG
Cuts at: 544 910 2137 2884 2904 544
Size: 366 1227 747 20 3906
Fragments arranged by size:
3906 1227 747 366 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2611 2611
Size: 6266
AlwNI CAG_nnn'CTG
Cuts at: 549 999 1764 1914 2022 3571 5138 549
Size: 450 765 150 108 1549 1567 1677
Fragments arranged by size:
1677 1567 1549 765 450 150 108
ApaI G_GGCC'C
Cuts at: 115 505 1494 2515 2761 115
Size: 390 989 1021 246 3620
Fragments arranged by size:
3620 1021 989 390 246
ApaLI G'TGCA_C
Cuts at: 5036 5036
Size: 6266
ApoI r'AATT_y
Cuts at: 2500 3270 5619 5875 2500
Size: 770 2349 256 2891
Fragments arranged by size:
2891 2349 770 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6266
AvaI C'yCGr_G
Cuts at: 249 332 1744 2179 3286 249
Size: 83 1412 435 1107 3229
Fragments arranged by size:
3229 1412 1107 435 83
AvrII C'CTAG_G
Cuts at: 2553 2553
Size: 6266
BamHI G'GATC_C
Cuts at: 2739 3291 2739
Size: 552 5714
BanI G'GyrC_C
Cuts at: 325 706 745 754 919 929 1018 1087 1126
Size: 381 39 9 165 10 89 69 39
Cuts at: 1126 1527 2142 3282 3696 3944 3979 325
Size: 401 615 1140 414 248 35 2612
Fragments arranged by size:
2612 1140 615 414 401 381 248 165
89 69 39 39 35 10 9
BanII G_rGCy'C
Cuts at: 115 341 505 1009 1281 1334 1494 2515 2761
Size: 226 164 504 272 53 160 1021 246
Cuts at: 2761 3174 3280 4310 115
Size: 413 106 1030 2071
Fragments arranged by size:
2071 1030 1021 504 413 272 246 226
164 160 106 53
BbeI G_GCGC'C
Cuts at: 749 1022 1091 3700 3948 749
Size: 273 69 2609 248 3067
Fragments arranged by size:
3067 2609 273 248 69
BbsI GAAGACnn'nnnn_
Cuts at: 572 1131 1713 572
Size: 559 582 5125
Fragments arranged by size:
5125 582 559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 243 277 2904 2938 243
Size: 34 2627 34 3571
Fragments arranged by size:
3571 2627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 207 2612 4164 4925 6266 207
Size: 2405 1552 761 1341 207
Fragments arranged by size:
2405 1552 1341 761 207
BclI T'GATC_A
Cuts at: 3131 3786 3131
Size: 655 5611
BglI GCCn_nnn'nGGC
Cuts at: 263 509 704 1202 1689 2122 263
Size: 246 195 498 487 433 4407
Fragments arranged by size:
4407 498 487 433 246 195
BglII A'GATC_T
Cuts at: 3781 5818 3781
Size: 2037 4229
BlpI GC'TnA_GC
Cuts at: 958 2023 2398 3378 958
Size: 1065 375 980 3846
Fragments arranged by size:
3846 1065 980 375
Bme1580I G_kGCm'C
Cuts at: 115 330 350 391 505 531 709 924 1494
Size: 215 20 41 114 26 178 215 570
Cuts at: 1494 1532 2515 2761 2835 2857 3891 3984 5040
Size: 38 983 246 74 22 1034 93 1056
Cuts at: 5040 115
Size: 1341
Fragments arranged by size:
1341 1056 1034 983 570 246 215 215
178 114 93 74 41 38 26 22
20
BmgBI CAC'GTC
Cuts at: 2327 2327
Size: 6266
BmrI ACTGGGnnnn_n'
Cuts at: 1193 3478 3559 3894 6038 1193
Size: 2285 81 335 2144 1421
Fragments arranged by size:
2285 2144 1421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 639 4633 639
Size: 3994 2272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 703 1021 1681 1786 1936 2017 2371 2581
Size: 94 318 660 105 150 81 354 210
Cuts at: 2581 2675 609
Size: 94 4200
Fragments arranged by size:
4200 660 354 318 210 150 105 94
94 81
Bpu10I CC'TnA_GC
Cuts at: 2065 2065
Size: 6266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2476 3442 4321 4813 5111 2476
Size: 966 879 492 298 3631
Fragments arranged by size:
3631 966 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 971 2194 2651 5970 971
Size: 1223 457 3319 1267
Fragments arranged by size:
3319 1267 1223 457
BsaAI yAC'GTr
Cuts at: 597 3023 3619 597
Size: 2426 596 3244
Fragments arranged by size:
3244 2426 596
BsaBI GATnn'nnATC
Cuts at: 6 427 3780 5817 6
Size: 421 3353 2037 455
Fragments arranged by size:
3353 2037 455 421
BsaHI Gr'CG_yC
Cuts at: 746 1019 1088 1883 2048 2114 3697 3945 4647
Size: 273 69 795 165 66 1583 248 702
Cuts at: 4647 6143 746
Size: 1496 869
Fragments arranged by size:
1583 1496 869 795 702 273 248 165
69 66
BsaWI w'CCGG_w
Cuts at: 365 2051 3455 3976 4654 4928 5075 5486 365
Size: 1686 1404 521 678 274 147 411 1145
Fragments arranged by size:
1686 1404 1145 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 473 503 473
Size: 30 6236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1002 1324 3073 1002
Size: 322 1749 4195
Fragments arranged by size:
4195 1749 322
BseYI C'CCAG_C
Cuts at: 121 702 1346 1371 1863 2217 2963 3123 3691
Size: 581 644 25 492 354 746 160 568
Cuts at: 3691 4449 5026 5522 121
Size: 758 577 496 865
Fragments arranged by size:
865 758 746 644 581 577 568 496
492 354 160 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1538 2272 2329 3128 874
Size: 664 734 57 799 4012
Fragments arranged by size:
4012 799 734 664 57
BsiEI CG_ry'CG
Cuts at: 104 182 275 1684 1854 3071 3808 3854 5062
Size: 78 93 1409 170 1217 737 46 1208
Cuts at: 5062 104
Size: 1308
Fragments arranged by size:
1409 1308 1217 1208 737 170 93 78
46
BsiHKAI G_wGCw'C
Cuts at: 341 1281 3280 4058 4248 5040 341
Size: 940 1999 778 190 792 1567
Fragments arranged by size:
1999 1567 940 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 201 679 2039 201
Size: 478 1360 4428
Fragments arranged by size:
4428 1360 478
Bsp1286I G_dGCh'C
Cuts at: 115 330 341 350 391 505 531 709 924
Size: 215 11 9 41 114 26 178 215
Cuts at: 924 1009 1281 1334 1494 1532 2515 2761 2835
Size: 85 272 53 160 38 983 246 74
Cuts at: 2835 2857 3174 3280 3891 3984 4058 4248 4310
Size: 22 317 106 611 93 74 190 62
Cuts at: 4310 5040 115
Size: 730 1341
Fragments arranged by size:
1341 983 730 611 317 272 246 215
215 190 178 160 114 106 93 85
74 74 62 53 41 38 26 22
11 9
BspEI T'CCGG_A
Cuts at: 2051 5486 2051
Size: 3435 2831
BspHI T'CATG_A
Cuts at: 1142 5442 1142
Size: 4300 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 861 984 989 1002 1341 3316 3530 3832
Size: 541 123 5 13 339 1975 214 302
Cuts at: 3832 4213 320
Size: 381 2373
Fragments arranged by size:
2373 1975 541 381 339 302 214 123
13 5
BsrBI CCG'CTC
Cuts at: 50 297 420 4558 4612 6096 6261 50
Size: 247 123 4138 54 1484 165 55
Fragments arranged by size:
4138 1484 247 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 612 1037 2589 2634 4178 612
Size: 425 1552 45 1544 2700
Fragments arranged by size:
2700 1552 1544 425 45
BsrFI r'CCGG_y
Cuts at: 181 1679 1702 2414 3066 4264 4445 4654 181
Size: 1498 23 712 652 1198 181 209 1793
Fragments arranged by size:
1793 1498 1198 712 652 209 181 23
BsrGI T'GTAC_A
Cuts at: 1304 1304
Size: 6266
BssHII G'CGCG_C
Cuts at: 128 128
Size: 6266
BssSI C'ACGA_G
Cuts at: 2119 2900 4537 4895 2119
Size: 781 1637 358 3490
Fragments arranged by size:
3490 1637 781 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3643 3643
Size: 6266
BstBI TT'CG_AA
Cuts at: 4627 4627
Size: 6266
BstEII G'GTnAC_C
Cuts at: 2280 2280
Size: 6266
BstXI CCAn_nnnn'nTGG
Cuts at: 1044 2629 3006 1044
Size: 1585 377 4304
Fragments arranged by size:
4304 1585 377
BstYI r'GATC_y
Cuts at: 1 2739 3291 3758 3781 4116 4362 5374 5460
Size: 2738 552 467 23 335 246 1012 86
Cuts at: 5460 5472 5818 1
Size: 12 346 449
Fragments arranged by size:
2738 1012 552 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 925 1359 1725 2606 2622 3101 107
Size: 818 434 366 881 16 479 3272
Fragments arranged by size:
3272 881 818 479 434 366 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4216 4397 4560 4216
Size: 181 163 5922
Fragments arranged by size:
5922 181 163
BtsI GCAGTG_nn'
Cuts at: 395 1713 3509 395
Size: 1318 1796 3152
Fragments arranged by size:
3152 1796 1318
DraI TTT'AAA
Cuts at: 3265 3265
Size: 6266
DraIII CAC_nnn'GTG
Cuts at: 1479 2841 1479
Size: 1362 4904
DrdI GACnn_nn'nnGTC
Cuts at: 3972 4830 3972
Size: 858 5408
EaeI y'GGCC_r
Cuts at: 179 450 491 1226 1388 1565 1681 3068 3098
Size: 271 41 735 162 177 116 1387 30
Cuts at: 3098 3851 4025 4416 4443 5788 179
Size: 753 174 391 27 1345 657
Fragments arranged by size:
1387 1345 753 735 657 391 271 177
174 162 116 41 30 27
EagI C'GGCC_G
Cuts at: 179 1681 3068 3851 179
Size: 1502 1387 783 2594
Fragments arranged by size:
2594 1502 1387 783
EarI CTCTTCn'nnn_
Cuts at: 265 3214 4289 4499 265
Size: 2949 1075 210 2032
Fragments arranged by size:
2949 2032 1075 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 189 989 1792 3226 4474 4784 4930 6076 189
Size: 800 803 1434 1248 310 146 1146 379
Fragments arranged by size:
1434 1248 1146 803 800 379 310 146
EcoICRI GAG'CTC
Cuts at: 339 1279 3278 339
Size: 940 1999 3327
Fragments arranged by size:
3327 1999 940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 703 1021 1681 1786 1936 2017 2287 2371
Size: 94 318 660 105 150 81 270 84
Cuts at: 2371 2581 2675 4091 4523 5270 5538 609
Size: 210 94 1416 432 747 268 1337
Fragments arranged by size:
1416 1337 747 660 432 318 270 268
210 150 105 94 94 84 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1900 1900
Size: 6266
EcoO109I rG'GnC_Cy
Cuts at: 112 501 502 828 1074 2183 2298 2543 2633
Size: 389 1 326 246 1109 115 245 90
Cuts at: 2633 2757 2758 3405 112
Size: 124 1 647 2973
Fragments arranged by size:
2973 1109 647 389 326 246 245 124
115 90 1 1
EcoRI G'AATT_C
Cuts at: 2500 3270 2500
Size: 770 5496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3174 4285 3174
Size: 1111 5155
FspI TGC'GCA
Cuts at: 4047 4047
Size: 6266
HaeII r_GCGC'y
Cuts at: 309 749 1022 1037 1091 2103 2128 3700 3948
Size: 440 273 15 54 1012 25 1572 248
Cuts at: 3948 4970 5949 309
Size: 1022 979 626
Fragments arranged by size:
1572 1022 1012 979 626 440 273 248
54 25 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 506 538 1836 1868 2349 2381 2648 2680 2692
Size: 32 1298 32 481 32 267 32 12
Cuts at: 2692 2724 3028 3060 4108 4138 4140 4170 506
Size: 32 304 32 1048 30 2 30 2602
Fragments arranged by size:
2602 1298 1048 481 304 267 32 32
32 32 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 3305 3305
Size: 6266
HindIII A'AGCT_T
Cuts at: 2424 2424
Size: 6266
KasI G'GCGC_C
Cuts at: 745 1018 1087 3696 3944 745
Size: 273 69 2609 248 3067
Fragments arranged by size:
3067 2609 273 248 69
KpnI G_GTAC'C
Cuts at: 3286 3286
Size: 6266
MluI A'CGCG_T
Cuts at: 5699 5699
Size: 6266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4937 5121 4937
Size: 184 6082
MscI TGG'CCA
Cuts at: 452 493 1228 1390 4027 452
Size: 41 735 162 2637 2691
Fragments arranged by size:
2691 2637 735 162 41
MslI CAynn'nnrTG
Cuts at: 407 433 544 910 1819 2074 2137 2278 2627
Size: 26 111 366 909 255 63 141 349
Cuts at: 2627 2884 2904 3004 4255 4382 407
Size: 257 20 100 1251 127 2291
Fragments arranged by size:
2291 1251 909 366 349 257 255 141
127 111 100 63 26 20
MspA1I CmG'CkG
Cuts at: 573 702 713 1540 1717 2313 2736 3377 3691
Size: 129 11 827 177 596 423 641 314
Cuts at: 3691 4051 5064 5309 573
Size: 360 1013 245 1530
Fragments arranged by size:
1530 1013 827 641 596 423 360 314
245 177 129 11
NaeI GCC'GGC
Cuts at: 183 1681 1704 3068 4447 183
Size: 1498 23 1364 1379 2002
Fragments arranged by size:
2002 1498 1379 1364 23
NarI GG'CG_CC
Cuts at: 746 1019 1088 3697 3945 746
Size: 273 69 2609 248 3067
Fragments arranged by size:
3067 2609 273 248 69
NcoI C'CATG_G
Cuts at: 107 1359 2622 107
Size: 1252 1263 3751
Fragments arranged by size:
3751 1263 1252
NdeI CA'TA_TG
Cuts at: 938 938
Size: 6266
NgoMIV G'CCGG_C
Cuts at: 181 1679 1702 3066 4445 181
Size: 1498 23 1364 1379 2002
Fragments arranged by size:
2002 1498 1379 1364 23
NspI r_CATG'y
Cuts at: 474 696 3319 3819 4726 474
Size: 222 2623 500 907 2014
Fragments arranged by size:
2623 2014 907 500 222
PasI CC'CwG_GG
Cuts at: 2695 2695
Size: 6266
PciI A'CATG_T
Cuts at: 470 4722 470
Size: 4252 2014
PflMI CCAn_nnn'nTGG
Cuts at: 1764 2247 1764
Size: 483 5783
PmeI GTTT'AAAC
Cuts at: 3265 3265
Size: 6266
PmlI CAC'GTG
Cuts at: 3023 3023
Size: 6266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1953 5405 5437 1921
Size: 32 3452 32 2750
Fragments arranged by size:
3452 2750 32 32
PpuMI rG'GwC_Cy
Cuts at: 828 1074 2298 2543 2633 828
Size: 246 1224 245 90 4461
Fragments arranged by size:
4461 1224 246 245 90
PshAI GACnn'nnGTC
Cuts at: 2548 2548
Size: 6266
PspOMI G'GGCC_C
Cuts at: 111 501 1490 2511 2757 111
Size: 390 989 1021 246 3620
Fragments arranged by size:
3620 1021 989 390 246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 721 753 1612 1644 721
Size: 32 859 32 5343
Fragments arranged by size:
5343 859 32 32
PstI C_TGCA'G
Cuts at: 510 2318 3313 3998 510
Size: 1808 995 685 2778
Fragments arranged by size:
2778 1808 995 685
PvuI CG_AT'CG
Cuts at: 104 1854 104
Size: 1750 4516
PvuII CAG'CTG
Cuts at: 702 1540 2313 2736 3691 4051 702
Size: 838 773 423 955 360 2917
Fragments arranged by size:
2917 955 838 773 423 360
RsrII CG'GwC_CG
Cuts at: 4461 4461
Size: 6266
SacI G_AGCT'C
Cuts at: 341 1281 3280 341
Size: 940 1999 3327
Fragments arranged by size:
3327 1999 940
SalI G'TCGA_C
Cuts at: 3303 3303
Size: 6266
SanDI GG'GwC_CC
Cuts at: 2543 2543
Size: 6266
SapI GCTCTTCn'nnn_
Cuts at: 3214 4289 4499 3214
Size: 1075 210 4981
Fragments arranged by size:
4981 1075 210
SbfI CC_TGCA'GG
Cuts at: 510 3313 510
Size: 2803 3463
ScaI AGT'ACT
Cuts at: 5783 5783
Size: 6266
SfcI C'TryA_G
Cuts at: 33 506 1629 2314 3309 3994 4987 5178 33
Size: 473 1123 685 995 685 993 191 1121
Fragments arranged by size:
1123 1121 995 993 685 685 473 191
SfoI GGC'GCC
Cuts at: 747 1020 1089 3698 3946 747
Size: 273 69 2609 248 3067
Fragments arranged by size:
3067 2609 273 248 69
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6266
SmaI CCC'GGG
Cuts at: 251 334 2181 3288 251
Size: 83 1847 1107 3229
Fragments arranged by size:
3229 1847 1107 83
SmlI C'TyrA_G
Cuts at: 2491 3421 4336 4828 5090 2491
Size: 930 915 492 262 3667
Fragments arranged by size:
3667 930 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6266
SphI G_CATG'C
Cuts at: 3319 3819 3319
Size: 500 5766
StyI C'CwwG_G
Cuts at: 107 840 1359 1523 2553 2622 3400 3754 107
Size: 733 519 164 1030 69 778 354 2619
Fragments arranged by size:
2619 1030 778 733 519 354 164 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 556 739 1055 2437 2960 3094 3265 3849
Size: 295 183 316 1382 523 134 171 584
Cuts at: 3849 3982 4441 4650 261
Size: 133 459 209 1877
Fragments arranged by size:
1877 1382 584 523 459 316 295 209
183 171 134 133
TatI w'GTAC_w
Cuts at: 737 1304 2036 5781 737
Size: 567 732 3745 1222
Fragments arranged by size:
3745 1222 732 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 473 505 2332 2364 5783 5815 473
Size: 32 1827 32 3419 32 924
Fragments arranged by size:
3419 1827 924 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2947 4063 2947
Size: 1116 5150
XbaI T'CTAG_A
Cuts at: 3297 3297
Size: 6266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3003 3761 3003
Size: 758 5508
XmaI C'CCGG_G
Cuts at: 249 332 2179 3286 249
Size: 83 1847 1107 3229
Fragments arranged by size:
3229 1847 1107 83
XmnI GAAnn'nnTTC
Cuts at: 2227 2227
Size: 6266
ZraI GAC'GTC
Cuts at: 2049 2049
Size: 6266
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI BaeI BbvCI BfrBI BmtI
BsiWI BsmI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PfoI PsiI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI