Restriction map for
pF1KE2307
(Circular) (Six-base) MAPSORT of: pF1KE2307.seq Check: 934 from: 1 to: 4019
pF1KE2307 4019 bp
With 182 enzymes: SgfI *
November 16, 2010 13:28 ..
AccI GT'mk_AC
Cuts at: 534 1057 534
Size: 523 3496
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1844 2276 3023 3291 865
Size: 979 432 747 268 1593
Fragments arranged by size:
1593 979 747 432 268
AfeI AGC'GCT
Cuts at: 934 934
Size: 4019
AflIII A'CryG_T
Cuts at: 717 1371 2475 3452 717
Size: 654 1104 977 1284
Fragments arranged by size:
1284 1104 977 654
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 694 1324 2891 694
Size: 630 1567 1822
Fragments arranged by size:
1822 1567 630
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 388 1023 3372 3628 388
Size: 635 2349 256 779
Fragments arranged by size:
2349 779 635 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 1039 1039
Size: 4019
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 470 1035 1449 1697 1732 470
Size: 565 414 248 35 2757
Fragments arranged by size:
2757 565 414 248 35
BanII G_rGCy'C
Cuts at: 795 1033 2063 795
Size: 238 1030 2751
Fragments arranged by size:
2751 1030 238
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbvCI CC'TCA_GC
Cuts at: 834 834
Size: 4019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3985
BciVI GTATCCnnnnn_n'
Cuts at: 926 1917 2678 4019 926
Size: 991 761 1341 926
Fragments arranged by size:
1341 991 926 761
BclI T'GATC_A
Cuts at: 456 1539 456
Size: 1083 2936
BglII A'GATC_T
Cuts at: 550 1534 3571 550
Size: 984 2037 998
Fragments arranged by size:
2037 998 984
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 1644 1737 2793 1644
Size: 93 1056 2870
Fragments arranged by size:
2870 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 394 1231 1312 1647 3791 394
Size: 837 81 335 2144 622
Fragments arranged by size:
2144 837 622 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
Bpu10I CC'TnA_GC
Cuts at: 834 834
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 1195 2074 2566 2864 615
Size: 580 879 492 298 1770
Fragments arranged by size:
1770 879 580 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 597 627 597
Size: 30 3989
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 618 618
Size: 4019
BseYI C'CCAG_C
Cuts at: 788 1444 2202 2779 3275 788
Size: 656 758 577 496 1532
Fragments arranged by size:
1532 758 656 577 496
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1811 2001 2793 1033
Size: 778 190 792 2259
Fragments arranged by size:
2259 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 795 1033 1644 1737 1811 2001 2063 2793 795
Size: 238 611 93 74 190 62 730 2021
Fragments arranged by size:
2021 730 611 238 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 1283 1585 1966 1069
Size: 214 302 381 3122
Fragments arranged by size:
3122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 2017 2198 2407 2017
Size: 181 209 3629
Fragments arranged by size:
3629 209 181
BssSI C'ACGA_G
Cuts at: 149 2290 2648 149
Size: 2141 358 1520
Fragments arranged by size:
2141 1520 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 1396
Size: 4019
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstXI CCAn_nnnn'nTGG
Cuts at: 756 756
Size: 4019
BstYI r'GATC_y
Cuts at: 1 550 1044 1511 1534 1869 2115 3127 3213
Size: 549 494 467 23 335 246 1012 86
Cuts at: 3213 3225 3571 1
Size: 12 346 449
Fragments arranged by size:
1012 549 494 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 1262 1262
Size: 4019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 495 530 495
Size: 35 3984
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 278 758 1604 1778 2169 2196 3541 278
Size: 480 846 174 391 27 1345 756
Fragments arranged by size:
1345 846 756 480 391 174 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 507 707 2042 2252 507
Size: 200 1335 210 2274
Fragments arranged by size:
2274 1335 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1844 2276 3023 3291 865
Size: 979 432 747 268 1593
Fragments arranged by size:
1593 979 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 844 1158 844
Size: 314 3705
EcoRI G'AATT_C
Cuts at: 388 1023 388
Size: 635 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 966 2038 966
Size: 1072 2947
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 936 1453 1701 2723 3702 936
Size: 517 248 1022 979 1253
Fragments arranged by size:
1253 1022 979 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1861 1891 1893 1923 1861
Size: 30 2 30 3957
Fragments arranged by size:
3957 30 30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MfeI C'AATT_G
Cuts at: 656 812 656
Size: 156 3863
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 530 2690 2874 530
Size: 2160 184 1675
Fragments arranged by size:
2160 1675 184
MscI TGG'CCA
Cuts at: 280 760 1780 280
Size: 480 1020 2519
Fragments arranged by size:
2519 1020 480
MslI CAynn'nnrTG
Cuts at: 2008 2135 2008
Size: 127 3892
MspA1I CmG'CkG
Cuts at: 1130 1444 1804 2817 3062 1130
Size: 314 360 1013 245 2087
Fragments arranged by size:
2087 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NspI r_CATG'y
Cuts at: 721 1072 1572 2479 721
Size: 351 500 907 2261
Fragments arranged by size:
2261 907 500 351
PciI A'CATG_T
Cuts at: 717 2475 717
Size: 1758 2261
PfoI T'CCnGG_A
Cuts at: 483 483
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PpuMI rG'GwC_Cy
Cuts at: 844 844
Size: 4019
PshAI GACnn'nnGTC
Cuts at: 496 496
Size: 4019
PstI C_TGCA'G
Cuts at: 1066 1751 1066
Size: 685 3334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 1444 1804 1444
Size: 360 3659
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 707 2042 2252 707
Size: 1335 210 2474
Fragments arranged by size:
2474 1335 210
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 695 1062 1747 2740 2931 33
Size: 662 367 685 993 191 1121
Fragments arranged by size:
1121 993 685 662 367 191
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 630 1174 2089 2581 2843 630
Size: 544 915 492 262 1806
Fragments arranged by size:
1806 915 544 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
SspI AAT'ATT
Cuts at: 432 959 432
Size: 527 3492
StyI C'CwwG_G
Cuts at: 1153 1507 1153
Size: 354 3665
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 989 1602 1735 2194 2403 989
Size: 613 133 459 209 2605
Fragments arranged by size:
2605 613 459 209 133
TatI w'GTAC_w
Cuts at: 3534 3534
Size: 4019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 481 1514 481
Size: 1033 2986
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI