Restriction map for
pF1KE2304
(Circular) (Six-base) MAPSORT of: pF1KE2304.seq Check: 4312 from: 1 to: 4013
pF1KE2304 4013 bp
With 182 enzymes: SgfI *
November 10, 2010 10:12 ..
AccI GT'mk_AC
Cuts at: 524 926 1051 524
Size: 402 125 3486
Fragments arranged by size:
3486 402 125
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2270 3017 3285 1838
Size: 432 747 268 2566
Fragments arranged by size:
2566 747 432 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 141 141
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 223 696 1318 2885 223
Size: 473 622 1567 1351
Fragments arranged by size:
1567 1351 622 473
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 880 895 961 1017 3366 3622 880
Size: 15 66 56 2349 256 1271
Fragments arranged by size:
2349 1271 256 66 56 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 1033 1033
Size: 4013
AvrII C'CTAG_G
Cuts at: 138 138
Size: 4013
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 1027 2057 1027
Size: 1030 2983
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 4013
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 1638 1731 2787 1638
Size: 93 1056 2864
Fragments arranged by size:
2864 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3717 3717
Size: 4013
BsaAI yAC'GTr
Cuts at: 1366 1366
Size: 4013
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 4013
BseYI C'CCAG_C
Cuts at: 1438 2196 2773 3269 1438
Size: 758 577 496 2182
Fragments arranged by size:
2182 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
BsmI GAATG_Cn'
Cuts at: 834 834
Size: 4013
Bsp1286I G_dGCh'C
Cuts at: 1027 1638 1731 1805 1995 2057 2787 1027
Size: 611 93 74 190 62 730 2253
Fragments arranged by size:
2253 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 975 3189 975
Size: 2214 1799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 1063 1277 1579 1960 491
Size: 572 214 302 381 2544
Fragments arranged by size:
2544 572 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 834 1925 834
Size: 1091 2922
BsrFI r'CCGG_y
Cuts at: 2011 2192 2401 2011
Size: 181 209 3623
Fragments arranged by size:
3623 209 181
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 1038 1505 1528 1863 2109 3121 3207 3219
Size: 1037 467 23 335 246 1012 86 12
Cuts at: 3219 3565 1
Size: 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 476 723 476
Size: 247 3766
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 125 1256 125
Size: 1131 2882
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 227 537 1598 1772 2163 2190 3535 227
Size: 310 1061 174 391 27 1345 705
Fragments arranged by size:
1345 1061 705 391 310 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1838 2270 3017 3285 491
Size: 1347 432 747 268 1219
Fragments arranged by size:
1347 1219 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 693 1152 693
Size: 459 3554
EcoRI G'AATT_C
Cuts at: 880 1017 880
Size: 137 3876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 584 616 619 651 837 869 976 1008 1855
Size: 32 3 32 186 32 107 32 847
Cuts at: 1855 1885 1887 1917 584
Size: 30 2 30 2680
Fragments arranged by size:
2680 847 186 107 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 2684 2868 323
Size: 2361 184 1468
Fragments arranged by size:
2361 1468 184
MscI TGG'CCA
Cuts at: 229 1774 229
Size: 1545 2468
MslI CAynn'nnrTG
Cuts at: 320 443 839 890 905 2002 2129 320
Size: 123 396 51 15 1097 127 2204
Fragments arranged by size:
2204 1097 396 127 123 51 15
MspA1I CmG'CkG
Cuts at: 753 1124 1438 1798 2811 3056 753
Size: 371 314 360 1013 245 1710
Fragments arranged by size:
1710 1013 371 360 314 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NdeI CA'TA_TG
Cuts at: 531 531
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 693 693
Size: 4013
PshAI GACnn'nnGTC
Cuts at: 567 567
Size: 4013
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 753 1438 1798 753
Size: 685 360 2968
Fragments arranged by size:
2968 685 360
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 617 1056 1741 2734 2925 33
Size: 584 439 685 993 191 1121
Fragments arranged by size:
1121 993 685 584 439 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 138 1147 1501 138
Size: 1009 354 2650
Fragments arranged by size:
2650 1009 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 1729 2188 2397 1596
Size: 133 459 209 3212
Fragments arranged by size:
3212 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 456 488 775 807 3530 3562 456
Size: 32 287 32 2723 32 907
Fragments arranged by size:
2723 907 287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI