Restriction map for
pF1KE2300
(Circular) (Six-base) MAPSORT of: pF1KE2300.seq Check: 6201 from: 1 to: 5684
pF1KE2300 5684 bp
With 182 enzymes: SgfI *
November 10, 2010 10:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1545 1545
Size: 5684
AccI GT'mk_AC
Cuts at: 2722 2722
Size: 5684
Acc65I G'GTAC_C
Cuts at: 2700 2700
Size: 5684
AclI AA'CG_TT
Cuts at: 5524 5524
Size: 5684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1628 2546 3509 3941 4688 4956 1195
Size: 433 918 963 432 747 268 1923
Fragments arranged by size:
1923 963 918 747 433 432 268
AfeI AGC'GCT
Cuts at: 398 398
Size: 5684
AflIII A'CryG_T
Cuts at: 3036 4140 5117 3036
Size: 1104 977 3603
Fragments arranged by size:
3603 1104 977
AgeI A'CCGG_T
Cuts at: 4072 4072
Size: 5684
AleI CACnn'nnGTG
Cuts at: 2615 2615
Size: 5684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1671 1671
Size: 5684
AlwNI CAG_nnn'CTG
Cuts at: 2989 4556 2989
Size: 1567 4117
ApaLI G'TGCA_C
Cuts at: 4454 4454
Size: 5684
ApoI r'AATT_y
Cuts at: 227 440 529 663 711 876 1083 1620 1676
Size: 213 89 134 48 165 207 537 56
Cuts at: 1676 2371 2483 2565 2688 5037 5293 227
Size: 695 112 82 123 2349 256 618
Fragments arranged by size:
2349 695 618 537 256 213 207 165
134 123 112 89 82 56 48
AseI AT'TA_AT
Cuts at: 20 2321 20
Size: 2301 3383
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
AvaI C'yCGr_G
Cuts at: 252 2704 252
Size: 2452 3232
BamHI G'GATC_C
Cuts at: 2709 2709
Size: 5684
BanI G'GyrC_C
Cuts at: 2700 3114 3362 3397 2700
Size: 414 248 35 4987
Fragments arranged by size:
4987 414 248 35
BanII G_rGCy'C
Cuts at: 2462 2698 3728 2462
Size: 236 1030 4418
Fragments arranged by size:
4418 1030 236
BbeI G_GCGC'C
Cuts at: 3118 3366 3118
Size: 248 5436
BbsI GAAGACnn'nnnn_
Cuts at: 203 973 1189 2195 203
Size: 770 216 1006 3692
Fragments arranged by size:
3692 1006 770 216
BciVI GTATCCnnnnn_n'
Cuts at: 3582 4343 5684 3582
Size: 761 1341 3582
Fragments arranged by size:
3582 1341 761
BclI T'GATC_A
Cuts at: 3204 3204
Size: 5684
BfrBI ATG'CAT
Cuts at: 1658 1658
Size: 5684
BglII A'GATC_T
Cuts at: 2394 3199 5236 2394
Size: 805 2037 2842
Fragments arranged by size:
2842 2037 805
BlpI GC'TnA_GC
Cuts at: 2796 2796
Size: 5684
Bme1580I G_kGCm'C
Cuts at: 3309 3402 4458 3309
Size: 93 1056 4535
Fragments arranged by size:
4535 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 925 2896 2977 3312 5456 925
Size: 1971 81 335 2144 1153
Fragments arranged by size:
2144 1971 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4051 4051
Size: 5684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 2193 1496
Size: 697 4987
Bpu10I CC'TnA_GC
Cuts at: 2456 2456
Size: 5684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2860 3739 4231 4529 2860
Size: 879 492 298 4015
Fragments arranged by size:
4015 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5388 5388
Size: 5684
BsaAI yAC'GTr
Cuts at: 493 3037 493
Size: 2544 3140
BsaBI GATnn'nnATC
Cuts at: 6 2357 3198 5235 6
Size: 2351 841 2037 455
Fragments arranged by size:
2351 2037 841 455
BsaHI Gr'CG_yC
Cuts at: 3115 3363 4065 5561 3115
Size: 248 702 1496 3238
Fragments arranged by size:
3238 1496 702 248
BsaWI w'CCGG_w
Cuts at: 754 872 1335 1811 2873 3394 4072 4346 4493
Size: 118 463 476 1062 521 678 274 147
Cuts at: 4493 4904 754
Size: 411 1534
Fragments arranged by size:
1534 1062 678 521 476 463 411 274
147 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 712
Size: 5684
BseYI C'CCAG_C
Cuts at: 3109 3867 4444 4940 3109
Size: 758 577 496 3853
Fragments arranged by size:
3853 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3226 3272 4480 104
Size: 3122 46 1208 1308
Fragments arranged by size:
3122 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2462 2698 3476 3666 4458 2462
Size: 236 778 190 792 3688
Fragments arranged by size:
3688 792 778 236 190
BsmI GAATG_Cn'
Cuts at: 1027 1660 2087 1027
Size: 633 427 4624
Fragments arranged by size:
4624 633 427
Bsp1286I G_dGCh'C
Cuts at: 2462 2698 3309 3402 3476 3666 3728 4458 2462
Size: 236 611 93 74 190 62 730 3688
Fragments arranged by size:
3688 730 611 236 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1335 4904 1335
Size: 3569 2115
BspHI T'CATG_A
Cuts at: 2361 4860 2361
Size: 2499 3185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1545 1578 2734 2948 3250 3631 1545
Size: 33 1156 214 302 381 3598
Fragments arranged by size:
3598 1156 381 302 214 33
BsrBI CCG'CTC
Cuts at: 50 3976 4030 5514 5679 50
Size: 3926 54 1484 165 55
Fragments arranged by size:
3926 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3596 3596
Size: 5684
BsrFI r'CCGG_y
Cuts at: 3682 3863 4072 3682
Size: 181 209 5294
Fragments arranged by size:
5294 209 181
BssSI C'ACGA_G
Cuts at: 3955 4313 3955
Size: 358 5326
BstAPI GCAn_nnn'nTGC
Cuts at: 3061 3061
Size: 5684
BstBI TT'CG_AA
Cuts at: 4045 4045
Size: 5684
BstEII G'GTnAC_C
Cuts at: 868 868
Size: 5684
BstXI CCAn_nnnn'nTGG
Cuts at: 299 2029 2557 299
Size: 1730 528 3426
Fragments arranged by size:
3426 1730 528
BstYI r'GATC_y
Cuts at: 1 1045 2394 2709 3176 3199 3534 3780 4792
Size: 1044 1349 315 467 23 335 246 1012
Cuts at: 4792 4878 4890 5236 1
Size: 86 12 346 449
Fragments arranged by size:
1349 1044 1012 467 449 346 335 315
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2265 2265
Size: 5684
BtgI C'CryG_G
Cuts at: 107 1969 107
Size: 1862 3822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3634 3815 3978 3634
Size: 181 163 5340
Fragments arranged by size:
5340 181 163
BtsI GCAGTG_nn'
Cuts at: 2927 2927
Size: 5684
DraI TTT'AAA
Cuts at: 189 359 668 1088 1691 2423 2683 189
Size: 170 309 420 603 732 260 3190
Fragments arranged by size:
3190 732 603 420 309 260 170
DrdI GACnn_nn'nnGTC
Cuts at: 3390 4248 3390
Size: 858 4826
EaeI y'GGCC_r
Cuts at: 417 509 1563 2510 3269 3443 3834 3861 5206
Size: 92 1054 947 759 174 391 27 1345
Cuts at: 5206 417
Size: 895
Fragments arranged by size:
1345 1054 947 895 759 391 174 92
27
EagI C'GGCC_G
Cuts at: 3269 3269
Size: 5684
EarI CTCTTCn'nnn_
Cuts at: 918 3707 3917 918
Size: 2789 210 2685
Fragments arranged by size:
2789 2685 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3892 4202 4348 5494 3892
Size: 310 146 1146 4082
Fragments arranged by size:
4082 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2460 2696 2460
Size: 236 5448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1496 1628 2193 2546 3509 3941 4688 4956
Size: 301 132 565 353 963 432 747 268
Cuts at: 4956 1195
Size: 1923
Fragments arranged by size:
1923 963 747 565 432 353 301 268
132
EcoO109I rG'GnC_Cy
Cuts at: 2430 2823 2430
Size: 393 5291
EcoRI G'AATT_C
Cuts at: 440 876 2565 2688 440
Size: 436 1689 123 3436
Fragments arranged by size:
3436 1689 436 123
EcoRV GAT'ATC
Cuts at: 2447 2447
Size: 5684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1545 3703 1545
Size: 2158 3526
FspI TGC'GCA
Cuts at: 3465 3465
Size: 5684
HaeII r_GCGC'y
Cuts at: 400 3118 3366 4388 5367 400
Size: 2718 248 1022 979 717
Fragments arranged by size:
2718 1022 979 717 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1467 2245 2277 2307 2339 3526 3556 3558
Size: 32 778 32 30 32 1187 30 2
Cuts at: 3558 3588 1435
Size: 30 3531
Fragments arranged by size:
3531 1187 778 32 32 32 30 30
30 2
HincII GTy'rAC
Cuts at: 264 936 1553 2502 2723 264
Size: 672 617 949 221 3225
Fragments arranged by size:
3225 949 672 617 221
HindIII A'AGCT_T
Cuts at: 216 216
Size: 5684
HpaI GTT'AAC
Cuts at: 264 936 2502 264
Size: 672 1566 3446
Fragments arranged by size:
3446 1566 672
KasI G'GCGC_C
Cuts at: 3114 3362 3114
Size: 248 5436
KpnI G_GTAC'C
Cuts at: 2704 2704
Size: 5684
MfeI C'AATT_G
Cuts at: 143 389 143
Size: 246 5438
MluI A'CGCG_T
Cuts at: 5117 5117
Size: 5684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1890 4355 4539 1890
Size: 2465 184 3035
Fragments arranged by size:
3035 2465 184
MscI TGG'CCA
Cuts at: 419 511 1565 2512 3445 419
Size: 92 1054 947 933 2658
Fragments arranged by size:
2658 1054 947 933 92
MslI CAynn'nnrTG
Cuts at: 1434 1903 1909 2027 2474 2615 3673 3800 1434
Size: 469 6 118 447 141 1058 127 3318
Fragments arranged by size:
3318 1058 469 447 141 127 118 6
MspA1I CmG'CkG
Cuts at: 687 2795 3109 3469 4482 4727 687
Size: 2108 314 360 1013 245 1644
Fragments arranged by size:
2108 1644 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3865 3865
Size: 5684
NarI GG'CG_CC
Cuts at: 3115 3363 3115
Size: 248 5436
NcoI C'CATG_G
Cuts at: 107 1969 107
Size: 1862 3822
NgoMIV G'CCGG_C
Cuts at: 3863 3863
Size: 5684
NsiI A_TGCA'T
Cuts at: 1660 1660
Size: 5684
NspI r_CATG'y
Cuts at: 846 2344 2737 3237 4144 846
Size: 1498 393 500 907 2386
Fragments arranged by size:
2386 1498 907 500 393
PciI A'CATG_T
Cuts at: 4140 4140
Size: 5684
PmeI GTTT'AAAC
Cuts at: 2683 2683
Size: 5684
PmlI CAC'GTG
Cuts at: 493 493
Size: 5684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 4823
Size: 32 5652
PpuMI rG'GwC_Cy
Cuts at: 2430 2430
Size: 5684
PstI C_TGCA'G
Cuts at: 2731 3416 2731
Size: 685 4999
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5684
PvuII CAG'CTG
Cuts at: 687 3109 3469 687
Size: 2422 360 2902
Fragments arranged by size:
2902 2422 360
RsrII CG'GwC_CG
Cuts at: 3879 3879
Size: 5684
SacI G_AGCT'C
Cuts at: 2462 2698 2462
Size: 236 5448
SalI G'TCGA_C
Cuts at: 2721 2721
Size: 5684
SanDI GG'GwC_CC
Cuts at: 2430 2430
Size: 5684
SapI GCTCTTCn'nnn_
Cuts at: 3707 3917 3707
Size: 210 5474
SbfI CC_TGCA'GG
Cuts at: 2731 2731
Size: 5684
ScaI AGT'ACT
Cuts at: 5201 5201
Size: 5684
SfcI C'TryA_G
Cuts at: 33 242 614 2613 2727 3412 4405 4596 33
Size: 209 372 1999 114 685 993 191 1121
Fragments arranged by size:
1999 1121 993 685 372 209 191 114
SfoI GGC'GCC
Cuts at: 3116 3364 3116
Size: 248 5436
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
SmaI CCC'GGG
Cuts at: 2706 2706
Size: 5684
SmlI C'TyrA_G
Cuts at: 2839 3754 4246 4508 2839
Size: 915 492 262 4015
Fragments arranged by size:
4015 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5684
SphI G_CATG'C
Cuts at: 2737 3237 2737
Size: 500 5184
SspI AAT'ATT
Cuts at: 1926 1926
Size: 5684
StyI C'CwwG_G
Cuts at: 107 1481 1969 2513 2818 3172 107
Size: 1374 488 544 305 354 2619
Fragments arranged by size:
2619 1374 544 488 354 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3267 3400 3859 4068 3267
Size: 133 459 209 4883
Fragments arranged by size:
4883 459 209 133
TatI w'GTAC_w
Cuts at: 1895 2279 5199 1895
Size: 384 2920 2380
Fragments arranged by size:
2920 2380 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5201 5233 5201
Size: 32 5652
Tth111I GACn'n_nGTC
Cuts at: 3481 3481
Size: 5684
XbaI T'CTAG_A
Cuts at: 1012 2715 1012
Size: 1703 3981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3179 3179
Size: 5684
XmaI C'CCGG_G
Cuts at: 2704 2704
Size: 5684
XmnI GAAnn'nnTTC
Cuts at: 398 1065 1680 398
Size: 667 615 4402
Fragments arranged by size:
4402 667 615
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI