Restriction map for
pF1KE2267
(Circular) (Six-base) MAPSORT of: pF1KE2267.seq Check: 4241 from: 1 to: 5957
pF1KE2267 5957 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1817 2995 1817
Size: 1178 4779
Acc65I G'GTAC_C
Cuts at: 2973 2973
Size: 5957
AclI AA'CG_TT
Cuts at: 5797 5797
Size: 5957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1603 2114 3782 4214 4961 5229 637
Size: 966 511 1668 432 747 268 1365
Fragments arranged by size:
1668 1365 966 747 511 432 268
AflIII A'CryG_T
Cuts at: 834 836 2324 2869 3309 4413 5390 834
Size: 2 1488 545 440 1104 977 1401
Fragments arranged by size:
1488 1401 1104 977 545 440 2
AgeI A'CCGG_T
Cuts at: 4345 4345
Size: 5957
AleI CACnn'nnGTG
Cuts at: 2858 2858
Size: 5957
AlwNI CAG_nnn'CTG
Cuts at: 2101 2863 3262 4829 2101
Size: 762 399 1567 3229
Fragments arranged by size:
3229 1567 762 399
ApaI G_GGCC'C
Cuts at: 1296 1779 1296
Size: 483 5474
ApaLI G'TGCA_C
Cuts at: 4727 4727
Size: 5957
ApoI r'AATT_y
Cuts at: 1543 1876 2769 2961 5310 5566 1543
Size: 333 893 192 2349 256 1934
Fragments arranged by size:
2349 1934 893 333 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 5957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
AvaI C'yCGr_G
Cuts at: 240 619 1383 1657 2977 240
Size: 379 764 274 1320 3220
Fragments arranged by size:
3220 1320 764 379 274
BamHI G'GATC_C
Cuts at: 223 1836 2982 223
Size: 1613 1146 3198
Fragments arranged by size:
3198 1613 1146
BanI G'GyrC_C
Cuts at: 2443 2542 2973 3387 3635 3670 2443
Size: 99 431 414 248 35 4730
Fragments arranged by size:
4730 431 414 248 99 35
BanII G_rGCy'C
Cuts at: 241 1296 1779 2744 2971 4001 241
Size: 1055 483 965 227 1030 2197
Fragments arranged by size:
2197 1055 1030 965 483 227
BbeI G_GCGC'C
Cuts at: 3391 3639 3391
Size: 248 5709
BbsI GAAGACnn'nnnn_
Cuts at: 2015 2319 2924 2015
Size: 304 605 5048
Fragments arranged by size:
5048 605 304
BbvCI CC'TCA_GC
Cuts at: 2662 2662
Size: 5957
BciVI GTATCCnnnnn_n'
Cuts at: 3855 4616 5957 3855
Size: 761 1341 3855
Fragments arranged by size:
3855 1341 761
BclI T'GATC_A
Cuts at: 3477 3477
Size: 5957
BfrBI ATG'CAT
Cuts at: 1687 1687
Size: 5957
BglII A'GATC_T
Cuts at: 2357 3472 5509 2357
Size: 1115 2037 2805
Fragments arranged by size:
2805 2037 1115
BlpI GC'TnA_GC
Cuts at: 3069 3069
Size: 5957
Bme1580I G_kGCm'C
Cuts at: 1296 1779 3582 3675 4731 1296
Size: 483 1803 93 1056 2522
Fragments arranged by size:
2522 1803 1056 483 93
BmrI ACTGGGnnnn_n'
Cuts at: 1188 3169 3250 3585 5729 1188
Size: 1981 81 335 2144 1416
Fragments arranged by size:
2144 1981 1416 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1057 4324 1057
Size: 3267 2690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 1654 1944 1333
Size: 321 290 5346
Fragments arranged by size:
5346 321 290
Bpu10I CC'TnA_GC
Cuts at: 2662 2662
Size: 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 3133 4012 4504 4802 1010
Size: 2123 879 492 298 2165
Fragments arranged by size:
2165 2123 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2118 5661 2118
Size: 3543 2414
BsaAI yAC'GTr
Cuts at: 837 3310 837
Size: 2473 3484
BsaBI GATnn'nnATC
Cuts at: 6 3471 5508 6
Size: 3465 2037 455
Fragments arranged by size:
3465 2037 455
BsaHI Gr'CG_yC
Cuts at: 811 2316 3388 3636 4338 5834 811
Size: 1505 1072 248 702 1496 934
Fragments arranged by size:
1505 1496 1072 934 702 248
BsaWI w'CCGG_w
Cuts at: 3146 3667 4345 4619 4766 5177 3146
Size: 521 678 274 147 411 3926
Fragments arranged by size:
3926 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1174 1204 1174
Size: 30 5927
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 2712 301
Size: 2411 3546
BseYI C'CCAG_C
Cuts at: 413 3382 4140 4717 5213 413
Size: 2969 758 577 496 1157
Fragments arranged by size:
2969 1157 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 766 2722 186
Size: 580 1956 3421
Fragments arranged by size:
3421 1956 580
BsiEI CG_ry'CG
Cuts at: 104 181 1173 3499 3545 4753 104
Size: 77 992 2326 46 1208 1308
Fragments arranged by size:
2326 1308 1208 992 77 46
BsiHKAI G_wGCw'C
Cuts at: 1073 1088 2075 2971 3749 3939 4731 1073
Size: 15 987 896 778 190 792 2299
Fragments arranged by size:
2299 987 896 792 778 190 15
BsmI GAATG_Cn'
Cuts at: 796 2717 796
Size: 1921 4036
BsmBI CGTCTCn'nnnn_
Cuts at: 637 637
Size: 5957
Bsp1286I G_dGCh'C
Cuts at: 241 1073 1088 1296 1779 2075 2744 2971 3582
Size: 832 15 208 483 296 669 227 611
Cuts at: 3582 3675 3749 3939 4001 4731 241
Size: 93 74 190 62 730 1467
Fragments arranged by size:
1467 832 730 669 611 483 296 227
208 190 93 74 62 15
BspEI T'CCGG_A
Cuts at: 5177 5177
Size: 5957
BspHI T'CATG_A
Cuts at: 5133 5133
Size: 5957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 3007 3221 3523 3904 158
Size: 2849 214 302 381 2211
Fragments arranged by size:
2849 2211 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4249 4303 5787 5952 50
Size: 4199 54 1484 165 55
Fragments arranged by size:
4199 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1052 2127 3869 1052
Size: 1075 1742 3140
Fragments arranged by size:
3140 1742 1075
BsrFI r'CCGG_y
Cuts at: 3955 4136 4345 3955
Size: 181 209 5567
Fragments arranged by size:
5567 209 181
BssSI C'ACGA_G
Cuts at: 1720 2068 4228 4586 1720
Size: 348 2160 358 3091
Fragments arranged by size:
3091 2160 358 348
BstAPI GCAn_nnn'nTGC
Cuts at: 3334 3334
Size: 5957
BstBI TT'CG_AA
Cuts at: 4318 4318
Size: 5957
BstEII G'GTnAC_C
Cuts at: 688 688
Size: 5957
BstYI r'GATC_y
Cuts at: 1 223 1272 1836 2125 2357 2982 3449 3472
Size: 222 1049 564 289 232 625 467 23
Cuts at: 3472 3807 4053 5065 5151 5163 5509 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1049 1012 625 564 467 449 346 335
289 246 232 222 86 23 12
BtgI C'CryG_G
Cuts at: 132 181 1210 132
Size: 49 1029 4879
Fragments arranged by size:
4879 1029 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3907 4088 4251 3907
Size: 181 163 5613
Fragments arranged by size:
5613 181 163
BtsI GCAGTG_nn'
Cuts at: 169 1088 1117 2630 3200 169
Size: 919 29 1513 570 2926
Fragments arranged by size:
2926 1513 919 570 29
DraI TTT'AAA
Cuts at: 1527 1941 2513 2956 1527
Size: 414 572 443 4528
Fragments arranged by size:
4528 572 443 414
DraIII CAC_nnn'GTG
Cuts at: 1077 1077
Size: 5957
DrdI GACnn_nn'nnGTC
Cuts at: 3663 4521 3663
Size: 858 5099
EaeI y'GGCC_r
Cuts at: 178 1166 2471 2582 3542 3716 4107 4134 5479
Size: 988 1305 111 960 174 391 27 1345
Cuts at: 5479 178
Size: 656
Fragments arranged by size:
1345 1305 988 960 656 391 174 111
27
EagI C'GGCC_G
Cuts at: 178 3542 178
Size: 3364 2593
EarI CTCTTCn'nnn_
Cuts at: 1383 1669 1991 3980 4190 1383
Size: 286 322 1989 210 3150
Fragments arranged by size:
3150 1989 322 286 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 910 1096 4165 4475 4621 5767 910
Size: 186 3069 310 146 1146 1100
Fragments arranged by size:
3069 1146 1100 310 186 146
EcoICRI GAG'CTC
Cuts at: 2969 2969
Size: 5957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1333 1603 1654 1944 2114 3782 4214 4961
Size: 696 270 51 290 170 1668 432 747
Cuts at: 4961 5229 637
Size: 268 1365
Fragments arranged by size:
1668 1365 747 696 432 290 270 268
170 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1300 1300
Size: 5957
EcoO109I rG'GnC_Cy
Cuts at: 170 1292 1293 1775 2571 2814 3096 170
Size: 1122 1 482 796 243 282 3031
Fragments arranged by size:
3031 1122 796 482 282 243 1
EcoRI G'AATT_C
Cuts at: 1543 2961 1543
Size: 1418 4539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 5957
FspI TGC'GCA
Cuts at: 3738 3738
Size: 5957
HaeII r_GCGC'y
Cuts at: 3391 3639 4661 5640 3391
Size: 248 1022 979 3708
Fragments arranged by size:
3708 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 394 426 1899 1931 2816 2848 3799 3829 3831
Size: 32 1473 32 885 32 951 30 2
Cuts at: 3831 3861 394
Size: 30 2490
Fragments arranged by size:
2490 1473 951 885 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1623 2996 1623
Size: 1373 4584
KasI G'GCGC_C
Cuts at: 3387 3635 3387
Size: 248 5709
KpnI G_GTAC'C
Cuts at: 2977 2977
Size: 5957
MluI A'CGCG_T
Cuts at: 5390 5390
Size: 5957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1157 1568 4628 4812 1022
Size: 135 411 3060 184 2167
Fragments arranged by size:
3060 2167 411 184 135
MscI TGG'CCA
Cuts at: 3718 3718
Size: 5957
MslI CAynn'nnrTG
Cuts at: 2329 2342 2590 2698 2858 2874 3946 4073 2329
Size: 13 248 108 160 16 1072 127 4213
Fragments arranged by size:
4213 1072 248 160 127 108 16 13
MspA1I CmG'CkG
Cuts at: 134 183 1178 3068 3382 3742 4755 5000 134
Size: 49 995 1890 314 360 1013 245 1091
Fragments arranged by size:
1890 1091 1013 995 360 314 245 49
NaeI GCC'GGC
Cuts at: 4138 4138
Size: 5957
NarI GG'CG_CC
Cuts at: 3388 3636 3388
Size: 248 5709
NcoI C'CATG_G
Cuts at: 1210 1210
Size: 5957
NgoMIV G'CCGG_C
Cuts at: 4136 4136
Size: 5957
NotI GC'GGCC_GC
Cuts at: 178 178
Size: 5957
NsiI A_TGCA'T
Cuts at: 1689 1689
Size: 5957
NspI r_CATG'y
Cuts at: 149 1107 2873 3010 3510 4417 149
Size: 958 1766 137 500 907 1689
Fragments arranged by size:
1766 1689 958 907 500 137
PciI A'CATG_T
Cuts at: 2869 4413 2869
Size: 1544 4413
PfoI T'CCnGG_A
Cuts at: 828 1257 1268 1832 828
Size: 429 11 564 4953
Fragments arranged by size:
4953 564 429 11
PmeI GTTT'AAAC
Cuts at: 2956 2956
Size: 5957
PmlI CAC'GTG
Cuts at: 837 837
Size: 5957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 5096
Size: 32 5925
PpuMI rG'GwC_Cy
Cuts at: 170 2571 2814 170
Size: 2401 243 3313
Fragments arranged by size:
3313 2401 243
PsiI TTA'TAA
Cuts at: 1967 1967
Size: 5957
PspOMI G'GGCC_C
Cuts at: 1292 1775 1292
Size: 483 5474
PstI C_TGCA'G
Cuts at: 1112 1442 2063 2433 3004 3689 1112
Size: 330 621 370 571 685 3380
Fragments arranged by size:
3380 685 621 571 370 330
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5957
PvuII CAG'CTG
Cuts at: 1178 3382 3742 1178
Size: 2204 360 3393
Fragments arranged by size:
3393 2204 360
RsrII CG'GwC_CG
Cuts at: 4152 4152
Size: 5957
SacI G_AGCT'C
Cuts at: 2971 2971
Size: 5957
SacII CC_GC'GG
Cuts at: 135 184 135
Size: 49 5908
SalI G'TCGA_C
Cuts at: 2994 2994
Size: 5957
SapI GCTCTTCn'nnn_
Cuts at: 1669 3980 4190 1669
Size: 2311 210 3436
Fragments arranged by size:
3436 2311 210
SbfI CC_TGCA'GG
Cuts at: 3004 3004
Size: 5957
ScaI AGT'ACT
Cuts at: 292 5474 292
Size: 5182 775
SfcI C'TryA_G
Cuts at: 33 1108 1438 2059 2402 2429 3000 3685 4678
Size: 1075 330 621 343 27 571 685 993
Cuts at: 4678 4869 33
Size: 191 1121
Fragments arranged by size:
1121 1075 993 685 621 571 343 330
191 27
SfoI GGC'GCC
Cuts at: 3389 3637 3389
Size: 248 5709
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
SmaI CCC'GGG
Cuts at: 242 621 1385 2979 242
Size: 379 764 1594 3220
Fragments arranged by size:
3220 1594 764 379
SmlI C'TyrA_G
Cuts at: 1025 3112 4027 4519 4781 1025
Size: 2087 915 492 262 2201
Fragments arranged by size:
2201 2087 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5957
SphI G_CATG'C
Cuts at: 149 3010 3510 149
Size: 2861 500 2596
Fragments arranged by size:
2861 2596 500
SrfI GCCC'GGGC
Cuts at: 242 242
Size: 5957
SspI AAT'ATT
Cuts at: 1739 2565 1739
Size: 826 5131
StyI C'CwwG_G
Cuts at: 1210 1642 2820 3091 3445 1210
Size: 432 1178 271 354 3722
Fragments arranged by size:
3722 1178 432 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 2032 3540 3673 4132 4341 676
Size: 1356 1508 133 459 209 2292
Fragments arranged by size:
2292 1508 1356 459 209 133
TatI w'GTAC_w
Cuts at: 290 1392 5472 290
Size: 1102 4080 775
Fragments arranged by size:
4080 1102 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 1363 1395 2610 2642 5474 5506 652
Size: 32 679 32 1215 32 2832 32 1103
Fragments arranged by size:
2832 1215 1103 679 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3754 3754
Size: 5957
XbaI T'CTAG_A
Cuts at: 2988 2988
Size: 5957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3452 3452
Size: 5957
XmaI C'CCGG_G
Cuts at: 240 619 1383 2977 240
Size: 379 764 1594 3220
Fragments arranged by size:
3220 1594 764 379
XmnI GAAnn'nnTTC
Cuts at: 1715 1715
Size: 5957
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI PacI
PasI PflMI PshAI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI StuI SwaI XhoI ZraI