Restriction map for
pF1KE2259
(Circular) (Six-base) MAPSORT of: pF1KE2259.seq Check: 7202 from: 1 to: 4619
pF1KE2259 4619 bp
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AccI GT'mk_AC
Cuts at: 266 1657 266
Size: 1391 3228
Acc65I G'GTAC_C
Cuts at: 975 1635 975
Size: 660 3959
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 2444 2876 3623 3891 625
Size: 1819 432 747 268 1353
Fragments arranged by size:
1819 1353 747 432 268
AfeI AGC'GCT
Cuts at: 469 469
Size: 4619
AflIII A'CryG_T
Cuts at: 161 494 1971 3075 4052 161
Size: 333 1477 1104 977 728
Fragments arranged by size:
1477 1104 977 728 333
AgeI A'CCGG_T
Cuts at: 3007 3007
Size: 4619
AleI CACnn'nnGTG
Cuts at: 493 493
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 528 1924 3491 528
Size: 1396 1567 1656
Fragments arranged by size:
1656 1567 1396
ApaLI G'TGCA_C
Cuts at: 1291 3389 1291
Size: 2098 2521
ApoI r'AATT_y
Cuts at: 504 1623 3972 4228 504
Size: 1119 2349 256 895
Fragments arranged by size:
2349 1119 895 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1150 1387 1639 1150
Size: 237 252 4130
Fragments arranged by size:
4130 252 237
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 975 1230 1635 2049 2297 2332 975
Size: 255 405 414 248 35 3262
Fragments arranged by size:
3262 414 405 255 248 35
BanII G_rGCy'C
Cuts at: 755 1633 2663 755
Size: 878 1030 2711
Fragments arranged by size:
2711 1030 878
BbeI G_GCGC'C
Cuts at: 1234 2053 2301 1234
Size: 819 248 3552
Fragments arranged by size:
3552 819 248
BbsI GAAGACnn'nnnn_
Cuts at: 406 921 1530 406
Size: 515 609 3495
Fragments arranged by size:
3495 609 515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4585
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 1010 2139 1010
Size: 1129 3490
BglI GCCn_nnn'nGGC
Cuts at: 1286 1286
Size: 4619
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 1731 1731
Size: 4619
Bme1580I G_kGCm'C
Cuts at: 1295 2244 2337 3393 1295
Size: 949 93 1056 2521
Fragments arranged by size:
2521 1056 949 93
BmgBI CAC'GTC
Cuts at: 300 300
Size: 4619
BmrI ACTGGGnnnn_n'
Cuts at: 984 1831 1912 2247 4391 984
Size: 847 81 335 2144 1212
Fragments arranged by size:
2144 1212 847 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1438 518
Size: 920 3699
Bpu10I CC'TnA_GC
Cuts at: 277 277
Size: 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2674 3166 3464 1795
Size: 879 492 298 2950
Fragments arranged by size:
2950 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 162 1972 162
Size: 1810 2809
BsaBI GATnn'nnATC
Cuts at: 6 2133 4170 6
Size: 2127 2037 455
Fragments arranged by size:
2127 2037 455
BsaHI Gr'CG_yC
Cuts at: 128 476 1231 2050 2298 3000 4496 128
Size: 348 755 819 248 702 1496 251
Fragments arranged by size:
1496 819 755 702 348 251 248
BsaWI w'CCGG_w
Cuts at: 1808 2329 3007 3281 3428 3839 1808
Size: 521 678 274 147 411 2588
Fragments arranged by size:
2588 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1582 1612 1582
Size: 30 4589
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 735 1435 735
Size: 700 3919
BseYI C'CCAG_C
Cuts at: 2044 2802 3379 3875 2044
Size: 758 577 496 2788
Fragments arranged by size:
2788 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 610 1354 1536 142
Size: 468 744 182 3225
Fragments arranged by size:
3225 744 468 182
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 836 1295 1364 1633 2411 2601 3393 836
Size: 459 69 269 778 190 792 2062
Fragments arranged by size:
2062 792 778 459 269 190 69
Bsp1286I G_dGCh'C
Cuts at: 755 836 1295 1364 1633 2244 2337 2411 2601
Size: 81 459 69 269 611 93 74 190
Cuts at: 2601 2663 3393 755
Size: 62 730 1981
Fragments arranged by size:
1981 730 611 459 269 190 93 81
74 69 62
BspEI T'CCGG_A
Cuts at: 3839 3839
Size: 4619
BspHI T'CATG_A
Cuts at: 3795 3795
Size: 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 824 1265 1669 1883 2185 2566 536
Size: 288 441 404 214 302 381 2589
Fragments arranged by size:
2589 441 404 381 302 288 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 516 1433 2531 516
Size: 917 1098 2604
Fragments arranged by size:
2604 1098 917
BsrFI r'CCGG_y
Cuts at: 2617 2798 3007 2617
Size: 181 209 4229
Fragments arranged by size:
4229 209 181
BsrGI T'GTAC_A
Cuts at: 1262 1262
Size: 4619
BssSI C'ACGA_G
Cuts at: 940 2890 3248 940
Size: 1950 358 2311
Fragments arranged by size:
2311 1950 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1340 1368 1996 1340
Size: 28 628 3963
Fragments arranged by size:
3963 628 28
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 1416 1587 1416
Size: 171 4448
BstYI r'GATC_y
Cuts at: 1 642 1412 1644 2111 2134 2469 2715 3727
Size: 641 770 232 467 23 335 246 1012
Cuts at: 3727 3813 3825 4171 1
Size: 86 12 346 449
Fragments arranged by size:
1012 770 641 467 449 346 335 246
232 86 23 12
BtgI C'CryG_G
Cuts at: 236 1226 1275 236
Size: 990 49 3580
Fragments arranged by size:
3580 990 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 594 1862 594
Size: 1268 3351
ClaI AT'CG_AT
Cuts at: 563 563
Size: 4619
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 143 239 420 1205 1329 1406 2204 2378 2769
Size: 96 181 785 124 77 798 174 391
Cuts at: 2769 2796 4141 143
Size: 27 1345 621
Fragments arranged by size:
1345 798 785 621 391 181 174 124
96 77 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 1138 2642 2852 1138
Size: 1504 210 2905
Fragments arranged by size:
2905 1504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 831 2827 3137 3283 4429 831
Size: 1996 310 146 1146 1021
Fragments arranged by size:
1996 1146 1021 310 146
EcoICRI GAG'CTC
Cuts at: 1631 1631
Size: 4619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 625 1438 2444 2876 3623 3891 518
Size: 107 813 1006 432 747 268 1246
Fragments arranged by size:
1246 1006 813 747 432 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 228 1420 228
Size: 1192 3427
EcoO109I rG'GnC_Cy
Cuts at: 614 696 1395 1758 614
Size: 82 699 363 3475
Fragments arranged by size:
3475 699 363 82
EcoRI G'AATT_C
Cuts at: 504 1623 504
Size: 1119 3500
EcoRV GAT'ATC
Cuts at: 666 666
Size: 4619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 142 471 1197 1226 1234 2053 2301 3323 4302
Size: 329 726 29 8 819 248 1022 979
Cuts at: 4302 142
Size: 459
Fragments arranged by size:
1022 979 819 726 459 329 248 29
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1560 1582 1592 1614 2461 2491 2493 2523 1560
Size: 22 10 22 847 30 2 30 3656
Fragments arranged by size:
3656 847 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 1658 1658
Size: 4619
HindIII A'AGCT_T
Cuts at: 190 190
Size: 4619
KasI G'GCGC_C
Cuts at: 1230 2049 2297 1230
Size: 819 248 3552
Fragments arranged by size:
3552 819 248
KpnI G_GTAC'C
Cuts at: 979 1639 979
Size: 660 3959
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3290 3474 3290
Size: 184 4435
MscI TGG'CCA
Cuts at: 241 422 1207 1408 2380 241
Size: 181 785 201 972 2480
Fragments arranged by size:
2480 972 785 201 181
MslI CAynn'nnrTG
Cuts at: 298 493 1567 2608 2735 298
Size: 195 1074 1041 127 2182
Fragments arranged by size:
2182 1074 1041 195 127
MspA1I CmG'CkG
Cuts at: 1191 1277 1451 1730 2044 2404 3417 3662 1191
Size: 86 174 279 314 360 1013 245 2148
Fragments arranged by size:
2148 1013 360 314 279 245 174 86
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 1231 2050 2298 1231
Size: 819 248 3552
Fragments arranged by size:
3552 819 248
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NspI r_CATG'y
Cuts at: 1672 2172 3079 1672
Size: 500 907 3212
Fragments arranged by size:
3212 907 500
PasI CC'CwG_GG
Cuts at: 897 897
Size: 4619
PciI A'CATG_T
Cuts at: 3075 3075
Size: 4619
PflMI CCAn_nnn'nTGG
Cuts at: 335 335
Size: 4619
PfoI T'CCnGG_A
Cuts at: 1415 1415
Size: 4619
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PmlI CAC'GTG
Cuts at: 162 162
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1474 1506 3758 3790 1474
Size: 32 2252 32 2303
Fragments arranged by size:
2303 2252 32 32
PpuMI rG'GwC_Cy
Cuts at: 614 696 1395 614
Size: 82 699 3838
Fragments arranged by size:
3838 699 82
PshAI GACnn'nnGTC
Cuts at: 1102 1102
Size: 4619
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1246 1278 1246
Size: 32 4587
PstI C_TGCA'G
Cuts at: 290 650 876 1055 1201 1480 1666 2351 290
Size: 360 226 179 146 279 186 685 2558
Fragments arranged by size:
2558 685 360 279 226 186 179 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 2044 2404 2044
Size: 360 4259
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1633 1633
Size: 4619
SacII CC_GC'GG
Cuts at: 1278 1278
Size: 4619
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SanDI GG'GwC_CC
Cuts at: 1395 1395
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 994 4136 994
Size: 3142 1477
SfcI C'TryA_G
Cuts at: 33 286 646 872 1051 1197 1476 1662 2347
Size: 253 360 226 179 146 279 186 685
Cuts at: 2347 3340 3531 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 360 279 253 226 191
186 179 146
SfoI GGC'GCC
Cuts at: 1232 2051 2299 1232
Size: 819 248 3552
Fragments arranged by size:
3552 819 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1152 1641 1152
Size: 489 4130
SmlI C'TyrA_G
Cuts at: 1774 2689 3181 3443 1774
Size: 915 492 262 2950
Fragments arranged by size:
2950 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
StyI C'CwwG_G
Cuts at: 224 638 1753 2107 224
Size: 414 1115 354 2736
Fragments arranged by size:
2736 1115 414 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 2202 2335 2794 3003 1524
Size: 678 133 459 209 3140
Fragments arranged by size:
3140 678 459 209 133
TatI w'GTAC_w
Cuts at: 992 1262 4134 992
Size: 270 2872 1477
Fragments arranged by size:
2872 1477 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1501 1533 4136 4168 1501
Size: 32 2603 32 1952
Fragments arranged by size:
2603 1952 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 2114 1014
Size: 1100 3519
XmaI C'CCGG_G
Cuts at: 1150 1639 1150
Size: 489 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PsiI PspOMI PspXI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI