Restriction map for
pF1KE2202
(Circular) (Six-base) MAPSORT of: pF1KE2202.seq Check: 5769 from: 1 to: 5942
pF1KE2202 5942 bp
With 182 enzymes: SgfI *
September 21, 2016 11:20 ..
AccI GT'mk_AC
Cuts at: 162 2553 2681 2980 162
Size: 2391 128 299 3124
Fragments arranged by size:
3124 2391 299 128
Acc65I G'GTAC_C
Cuts at: 2958 2958
Size: 5942
AclI AA'CG_TT
Cuts at: 5782 5782
Size: 5942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1658 1724 1919 2126 2849 3767 4199 4946
Size: 240 66 195 207 723 918 432 747
Cuts at: 4946 5214 1418
Size: 268 2146
Fragments arranged by size:
2146 918 747 723 432 268 240 207
195 66
AfeI AGC'GCT
Cuts at: 1603 1603
Size: 5942
AflIII A'CryG_T
Cuts at: 2092 2335 3294 4398 5375 2092
Size: 243 959 1104 977 2659
Fragments arranged by size:
2659 1104 977 959 243
AgeI A'CCGG_T
Cuts at: 4330 4330
Size: 5942
AlwNI CAG_nnn'CTG
Cuts at: 3247 4814 3247
Size: 1567 4375
ApaLI G'TGCA_C
Cuts at: 4712 4712
Size: 5942
ApoI r'AATT_y
Cuts at: 267 464 559 669 866 1012 1378 1465 1476
Size: 197 95 110 197 146 366 87 11
Cuts at: 1476 1673 1931 2946 5295 5551 267
Size: 197 258 1015 2349 256 658
Fragments arranged by size:
2349 1015 658 366 258 256 197 197
197 146 110 95 87 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 5942
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5942
AvaI C'yCGr_G
Cuts at: 2962 2962
Size: 5942
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 596 629 596
Size: 33 5909
BamHI G'GATC_C
Cuts at: 2967 2967
Size: 5942
BanI G'GyrC_C
Cuts at: 2958 3372 3620 3655 2958
Size: 414 248 35 5245
Fragments arranged by size:
5245 414 248 35
BanII G_rGCy'C
Cuts at: 2956 3986 2956
Size: 1030 4912
BbeI G_GCGC'C
Cuts at: 3376 3624 3376
Size: 248 5694
BbsI GAAGACnn'nnnn_
Cuts at: 1858 1858
Size: 5942
BciVI GTATCCnnnnn_n'
Cuts at: 1245 2688 3840 4601 5942 1245
Size: 1443 1152 761 1341 1245
Fragments arranged by size:
1443 1341 1245 1152 761
BclI T'GATC_A
Cuts at: 534 3462 534
Size: 2928 3014
BfrBI ATG'CAT
Cuts at: 1764 1764
Size: 5942
BglII A'GATC_T
Cuts at: 1051 1294 2011 3457 5494 1051
Size: 243 717 1446 2037 1499
Fragments arranged by size:
2037 1499 1446 717 243
BlpI GC'TnA_GC
Cuts at: 3054 3054
Size: 5942
Bme1580I G_kGCm'C
Cuts at: 177 642 3567 3660 4716 177
Size: 465 2925 93 1056 1403
Fragments arranged by size:
2925 1403 1056 465 93
BmrI ACTGGGnnnn_n'
Cuts at: 1207 2723 2852 3154 3235 3570 5714 1207
Size: 1516 129 302 81 335 2144 1435
Fragments arranged by size:
2144 1516 1435 335 302 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4309 4309
Size: 5942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2147 2411 1928
Size: 219 264 5459
Fragments arranged by size:
5459 264 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 3997 4489 4787 3118
Size: 879 492 298 4273
Fragments arranged by size:
4273 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1088 5646 1088
Size: 4558 1384
BsaAI yAC'GTr
Cuts at: 178 3295 178
Size: 3117 2825
BsaBI GATnn'nnATC
Cuts at: 6 2645 3456 5493 6
Size: 2639 811 2037 455
Fragments arranged by size:
2639 2037 811 455
BsaHI Gr'CG_yC
Cuts at: 1683 3373 3621 4323 5819 1683
Size: 1690 248 702 1496 1806
Fragments arranged by size:
1806 1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 118 2894 3131 3652 4330 4604 4751 5162 118
Size: 2776 237 521 678 274 147 411 898
Fragments arranged by size:
2776 898 678 521 411 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1901 1931 2390 2420 1901
Size: 30 459 30 5423
Fragments arranged by size:
5423 459 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1729 2378 1729
Size: 649 5293
BseYI C'CCAG_C
Cuts at: 3367 4125 4702 5198 3367
Size: 758 577 496 4111
Fragments arranged by size:
4111 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 2036 1106
Size: 930 5012
BsiEI CG_ry'CG
Cuts at: 104 3484 3530 4738 104
Size: 3380 46 1208 1308
Fragments arranged by size:
3380 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 2956 3734 3924 4716 1120
Size: 1836 778 190 792 2346
Fragments arranged by size:
2346 1836 792 778 190
BsmI GAATG_Cn'
Cuts at: 1329 1329
Size: 5942
Bsp1286I G_dGCh'C
Cuts at: 177 642 1120 2956 3567 3660 3734 3924 3986
Size: 465 478 1836 611 93 74 190 62
Cuts at: 3986 4716 177
Size: 730 1403
Fragments arranged by size:
1836 1403 730 611 478 465 190 93
74 62
BspEI T'CCGG_A
Cuts at: 2894 5162 2894
Size: 2268 3674
BspHI T'CATG_A
Cuts at: 1923 5118 1923
Size: 3195 2747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2992 3206 3508 3889 2992
Size: 214 302 381 5045
Fragments arranged by size:
5045 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4234 4288 5772 5937 50
Size: 4184 54 1484 165 55
Fragments arranged by size:
4184 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3854 3854
Size: 5942
BsrFI r'CCGG_y
Cuts at: 3940 4121 4330 3940
Size: 181 209 5552
Fragments arranged by size:
5552 209 181
BssSI C'ACGA_G
Cuts at: 4213 4571 4213
Size: 358 5584
BstAPI GCAn_nnn'nTGC
Cuts at: 338 3319 338
Size: 2981 2961
BstBI TT'CG_AA
Cuts at: 4303 4303
Size: 5942
BstEII G'GTnAC_C
Cuts at: 2797 2797
Size: 5942
BstXI CCAn_nnnn'nTGG
Cuts at: 1694 1694
Size: 5942
BstYI r'GATC_y
Cuts at: 1 1051 1294 2011 2501 2565 2622 2967 3434
Size: 1050 243 717 490 64 57 345 467
Cuts at: 3434 3457 3792 4038 5050 5136 5148 5494 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1050 1012 717 490 467 449 346 345
335 246 243 86 64 57 23 12
BstZ17I GTA'TAC
Cuts at: 2554 2682 2554
Size: 128 5814
BtgI C'CryG_G
Cuts at: 107 107
Size: 5942
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3892 4073 4236 3892
Size: 181 163 5598
Fragments arranged by size:
5598 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 3185 1118
Size: 2067 3875
DraI TTT'AAA
Cuts at: 1892 2941 1892
Size: 1049 4893
DrdI GACnn_nn'nnGTC
Cuts at: 3648 4506 3648
Size: 858 5084
EaeI y'GGCC_r
Cuts at: 2763 3527 3701 4092 4119 5464 2763
Size: 764 174 391 27 1345 3241
Fragments arranged by size:
3241 1345 764 391 174 27
EagI C'GGCC_G
Cuts at: 3527 3527
Size: 5942
EarI CTCTTCn'nnn_
Cuts at: 782 1490 1499 3965 4175 782
Size: 708 9 2466 210 2549
Fragments arranged by size:
2549 2466 708 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4150 4460 4606 5752 4150
Size: 310 146 1146 4340
Fragments arranged by size:
4340 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2954 2954
Size: 5942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1658 1724 1919 1928 2126 2147 2411 2849
Size: 240 66 195 9 198 21 264 438
Cuts at: 2849 3767 4199 4946 5214 1418
Size: 918 432 747 268 2146
Fragments arranged by size:
2146 918 747 438 432 268 264 240
198 195 66 21 9
EcoO109I rG'GnC_Cy
Cuts at: 2592 3081 2592
Size: 489 5453
EcoRI G'AATT_C
Cuts at: 2946 2946
Size: 5942
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1280 1423 1620 2180 2550 3961 1280
Size: 143 197 560 370 1411 3261
Fragments arranged by size:
3261 1411 560 370 197 143
FspI TGC'GCA
Cuts at: 3723 3723
Size: 5942
HaeII r_GCGC'y
Cuts at: 1605 3376 3624 4646 5625 1605
Size: 1771 248 1022 979 1922
Fragments arranged by size:
1922 1771 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 2422 2454 3784 3814 3816 3846 784
Size: 32 1606 32 1330 30 2 30 2880
Fragments arranged by size:
2880 1606 1330 32 32 30 30 2
HincII GTy'rAC
Cuts at: 163 2478 2790 2981 163
Size: 2315 312 191 3124
Fragments arranged by size:
3124 2315 312 191
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 5942
KasI G'GCGC_C
Cuts at: 3372 3620 3372
Size: 248 5694
KpnI G_GTAC'C
Cuts at: 2962 2962
Size: 5942
MluI A'CGCG_T
Cuts at: 5375 5375
Size: 5942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 2327 4613 4797 1010
Size: 1317 2286 184 2155
Fragments arranged by size:
2286 2155 1317 184
MscI TGG'CCA
Cuts at: 2765 3703 2765
Size: 938 5004
MslI CAynn'nnrTG
Cuts at: 542 3931 4058 542
Size: 3389 127 2426
Fragments arranged by size:
3389 2426 127
MspA1I CmG'CkG
Cuts at: 1396 3053 3367 3727 4740 4985 1396
Size: 1657 314 360 1013 245 2353
Fragments arranged by size:
2353 1657 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4123 4123
Size: 5942
NarI GG'CG_CC
Cuts at: 3373 3621 3373
Size: 248 5694
NcoI C'CATG_G
Cuts at: 107 107
Size: 5942
NgoMIV G'CCGG_C
Cuts at: 4121 4121
Size: 5942
NsiI A_TGCA'T
Cuts at: 1766 1766
Size: 5942
NspI r_CATG'y
Cuts at: 955 2096 2761 2995 3495 4402 955
Size: 1141 665 234 500 907 2495
Fragments arranged by size:
2495 1141 907 665 500 234
PciI A'CATG_T
Cuts at: 2092 4398 2092
Size: 2306 3636
PmeI GTTT'AAAC
Cuts at: 2941 2941
Size: 5942
PmlI CAC'GTG
Cuts at: 178 178
Size: 5942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5081 5113 5081
Size: 32 5910
PpuMI rG'GwC_Cy
Cuts at: 2592 2592
Size: 5942
PsiI TTA'TAA
Cuts at: 1018 2879 1018
Size: 1861 4081
PstI C_TGCA'G
Cuts at: 2989 3674 2989
Size: 685 5257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5942
PvuII CAG'CTG
Cuts at: 1396 3367 3727 1396
Size: 1971 360 3611
Fragments arranged by size:
3611 1971 360
RsrII CG'GwC_CG
Cuts at: 4137 4137
Size: 5942
SacI G_AGCT'C
Cuts at: 2956 2956
Size: 5942
SalI G'TCGA_C
Cuts at: 161 2979 161
Size: 2818 3124
SapI GCTCTTCn'nnn_
Cuts at: 1490 3965 4175 1490
Size: 2475 210 3257
Fragments arranged by size:
3257 2475 210
SbfI CC_TGCA'GG
Cuts at: 2989 2989
Size: 5942
ScaI AGT'ACT
Cuts at: 1247 2356 2420 2841 5459 1247
Size: 1109 64 421 2618 1730
Fragments arranged by size:
2618 1730 1109 421 64
SfcI C'TryA_G
Cuts at: 33 144 397 2985 3670 4663 4854 33
Size: 111 253 2588 685 993 191 1121
Fragments arranged by size:
2588 1121 993 685 253 191 111
SfoI GGC'GCC
Cuts at: 3374 3622 3374
Size: 248 5694
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5942
SmaI CCC'GGG
Cuts at: 2964 2964
Size: 5942
SmlI C'TyrA_G
Cuts at: 3097 4012 4504 4766 3097
Size: 915 492 262 4273
Fragments arranged by size:
4273 915 492 262
SpeI A'CTAG_T
Cuts at: 67 2289 67
Size: 2222 3720
SphI G_CATG'C
Cuts at: 955 2995 3495 955
Size: 2040 500 3402
Fragments arranged by size:
3402 2040 500
SspI AAT'ATT
Cuts at: 1818 2466 1818
Size: 648 5294
StyI C'CwwG_G
Cuts at: 107 3076 3430 107
Size: 2969 354 2619
Fragments arranged by size:
2969 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1226 1689 3525 3658 4117 4326 1226
Size: 463 1836 133 459 209 2842
Fragments arranged by size:
2842 1836 463 459 209 133
TatI w'GTAC_w
Cuts at: 262 694 1245 1878 2354 2418 2809 2839 5457
Size: 432 551 633 476 64 391 30 2618
Cuts at: 5457 262
Size: 747
Fragments arranged by size:
2618 747 633 551 476 432 391 64
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2390 2422 5459 5491 2390
Size: 32 3037 32 2841
Fragments arranged by size:
3037 2841 32 32
Tth111I GACn'n_nGTC
Cuts at: 3739 3739
Size: 5942
XbaI T'CTAG_A
Cuts at: 1339 2008 2973 1339
Size: 669 965 4308
Fragments arranged by size:
4308 965 669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 3437 1694
Size: 1743 4199
XmaI C'CCGG_G
Cuts at: 2962 2962
Size: 5942
XmnI GAAnn'nnTTC
Cuts at: 1674 2909 1674
Size: 1235 4707
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI