Restriction map for
pF1KE2142
(Circular) (Six-base) MAPSORT of: pF1KE2142.seq Check: 6474 from: 1 to: 5999
pF1KE2142 5999 bp
With 182 enzymes: SgfI *
May 26, 2015 10:14 ..
AccI GT'mk_AC
Cuts at: 3037 3037
Size: 5999
Acc65I G'GTAC_C
Cuts at: 1880 2617 3015 1880
Size: 737 398 4864
Fragments arranged by size:
4864 737 398
AclI AA'CG_TT
Cuts at: 5839 5839
Size: 5999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 3824 4256 5003 5271 1558
Size: 2266 432 747 268 2286
Fragments arranged by size:
2286 2266 747 432 268
AfeI AGC'GCT
Cuts at: 865 865
Size: 5999
AflIII A'CryG_T
Cuts at: 1670 2577 3351 4455 5432 1670
Size: 907 774 1104 977 2237
Fragments arranged by size:
2237 1104 977 907 774
AgeI A'CCGG_T
Cuts at: 4387 4387
Size: 5999
AhdI GACnn_n'nnGTC
Cuts at: 1803 1803
Size: 5999
AleI CACnn'nnGTG
Cuts at: 2434 2434
Size: 5999
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1507 1539 1507
Size: 32 5967
AlwNI CAG_nnn'CTG
Cuts at: 285 772 1260 1572 2665 3304 4871 285
Size: 487 488 312 1093 639 1567 1413
Fragments arranged by size:
1567 1413 1093 639 488 487 312
ApaI G_GGCC'C
Cuts at: 535 1904 2800 2924 535
Size: 1369 896 124 3610
Fragments arranged by size:
3610 1369 896 124
ApaLI G'TGCA_C
Cuts at: 124 1852 4769 124
Size: 1728 2917 1354
Fragments arranged by size:
2917 1728 1354
ApoI r'AATT_y
Cuts at: 3003 5352 5608 3003
Size: 2349 256 3394
Fragments arranged by size:
3394 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5999
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5999
AvaI C'yCGr_G
Cuts at: 644 685 692 722 859 1472 1867 1897 2089
Size: 41 7 30 137 613 395 30 192
Cuts at: 2089 2917 3019 644
Size: 828 102 3624
Fragments arranged by size:
3624 828 613 395 192 137 102 41
30 30 7
BamHI G'GATC_C
Cuts at: 3024 3024
Size: 5999
BanI G'GyrC_C
Cuts at: 209 363 522 589 1074 1211 1358 1504 1863
Size: 154 159 67 485 137 147 146 359
Cuts at: 1863 1880 2514 2617 2845 3015 3429 3677 3712
Size: 17 634 103 228 170 414 248 35
Cuts at: 3712 209
Size: 2496
Fragments arranged by size:
2496 634 485 414 359 248 228 170
159 154 147 146 137 103 67 35
17
BanII G_rGCy'C
Cuts at: 328 535 692 1535 1619 1699 1898 1904 2800
Size: 207 157 843 84 80 199 6 896
Cuts at: 2800 2924 3013 4043 328
Size: 124 89 1030 2284
Fragments arranged by size:
2284 1030 896 843 207 199 157 124
89 84 80 6
BbeI G_GCGC'C
Cuts at: 213 1078 1362 2849 3433 3681 213
Size: 865 284 1487 584 248 2531
Fragments arranged by size:
2531 1487 865 584 284 248
BbsI GAAGACnn'nnnn_
Cuts at: 1653 2432 1653
Size: 779 5220
BbvCI CC'TCA_GC
Cuts at: 655 1349 1462 1709 2972 655
Size: 694 113 247 1263 3682
Fragments arranged by size:
3682 1263 694 247 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1036 1070 1036
Size: 34 5965
BciVI GTATCCnnnnn_n'
Cuts at: 2800 3897 4658 5999 2800
Size: 1097 761 1341 2800
Fragments arranged by size:
2800 1341 1097 761
BclI T'GATC_A
Cuts at: 3519 3519
Size: 5999
BglI GCCn_nnn'nGGC
Cuts at: 346 761 1170 1240 1372 2750 2928 346
Size: 415 409 70 132 1378 178 3417
Fragments arranged by size:
3417 1378 415 409 178 132 70
BglII A'GATC_T
Cuts at: 3514 5551 3514
Size: 2037 3962
BlpI GC'TnA_GC
Cuts at: 2363 2966 3111 2363
Size: 603 145 5251
Fragments arranged by size:
5251 603 145
Bme1580I G_kGCm'C
Cuts at: 128 195 240 527 535 594 1377 1595 1856
Size: 67 45 287 8 59 783 218 261
Cuts at: 1856 1868 1904 2800 2924 3624 3717 4773 128
Size: 12 36 896 124 700 93 1056 1354
Fragments arranged by size:
1354 1056 896 783 700 287 261 218
124 93 67 59 45 36 12 8
BmgBI CAC'GTC
Cuts at: 1673 1673
Size: 5999
BmrI ACTGGGnnnn_n'
Cuts at: 622 3211 3292 3627 5771 622
Size: 2589 81 335 2144 850
Fragments arranged by size:
2589 2144 850 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 670 4366 670
Size: 3696 2303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 250 298 397 961 2167 2440 2526 217
Size: 33 48 99 564 1206 273 86 3690
Fragments arranged by size:
3690 1206 564 273 99 86 48 33
Bpu10I CC'TnA_GC
Cuts at: 655 827 935 1181 1349 1462 1709 2972 655
Size: 172 108 246 168 113 247 1263 3682
Fragments arranged by size:
3682 1263 247 246 172 168 113 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2767 3175 4054 4546 4844 2767
Size: 408 879 492 298 3922
Fragments arranged by size:
3922 879 492 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 463 2263 5703 376
Size: 87 1800 3440 672
Fragments arranged by size:
3440 1800 672 87
BsaAI yAC'GTr
Cuts at: 748 1851 2204 2580 3352 748
Size: 1103 353 376 772 3395
Fragments arranged by size:
3395 1103 772 376 353
BsaBI GATnn'nnATC
Cuts at: 6 1546 3513 5550 6
Size: 1540 1967 2037 455
Fragments arranged by size:
2037 1967 1540 455
BsaHI Gr'CG_yC
Cuts at: 210 1075 1359 1643 2572 2846 2951 3430 3678
Size: 865 284 284 929 274 105 479 248
Cuts at: 3678 4380 5876 210
Size: 702 1496 333
Fragments arranged by size:
1496 929 865 702 479 333 284 284
274 248 105
BsaWI w'CCGG_w
Cuts at: 2018 2822 3188 3709 4387 4661 4808 5219 2018
Size: 804 366 521 678 274 147 411 2798
Fragments arranged by size:
2798 804 678 521 411 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1612 1642 2794 2824 1612
Size: 30 1152 30 4787
Fragments arranged by size:
4787 1152 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 412 984 1993 2107 394
Size: 18 572 1009 114 4286
Fragments arranged by size:
4286 1009 572 114 18
BseYI C'CCAG_C
Cuts at: 239 703 729 1085 1925 2793 2896 2923 3424
Size: 464 26 356 840 868 103 27 501
Cuts at: 3424 4182 4759 5255 239
Size: 758 577 496 983
Fragments arranged by size:
983 868 840 758 577 501 496 464
356 103 27 26
BsiEI CG_ry'CG
Cuts at: 104 134 1244 2407 3541 3587 4795 104
Size: 30 1110 1163 1134 46 1208 1308
Fragments arranged by size:
1308 1208 1163 1134 1110 46 30
BsiHKAI G_wGCw'C
Cuts at: 128 1699 1856 2447 2694 3013 3791 3981 4773
Size: 1571 157 591 247 319 778 190 792
Cuts at: 4773 128
Size: 1354
Fragments arranged by size:
1571 1354 792 778 591 319 247 190
157
BsmBI CGTCTCn'nnnn_
Cuts at: 2107 2107
Size: 5999
Bsp1286I G_dGCh'C
Cuts at: 128 195 240 328 527 535 594 692 1377
Size: 67 45 88 199 8 59 98 685
Cuts at: 1377 1535 1595 1619 1699 1856 1868 1898 1904
Size: 158 60 24 80 157 12 30 6
Cuts at: 1904 2447 2694 2800 2924 3013 3624 3717 3791
Size: 543 247 106 124 89 611 93 74
Cuts at: 3791 3981 4043 4773 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 730 685 611 543 247 199 190
158 157 124 106 98 93 89 88
80 74 67 62 60 59 45 30
24 12 8 6
BspEI T'CCGG_A
Cuts at: 5219 5219
Size: 5999
BspHI T'CATG_A
Cuts at: 5175 5175
Size: 5999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1259 2048 3049 3263 3565 3946 1259
Size: 789 1001 214 302 381 3312
Fragments arranged by size:
3312 1001 789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 851 1050 2832 4291 4345 5829 5994
Size: 157 644 199 1782 1459 54 1484 165
Cuts at: 5994 50
Size: 55
Fragments arranged by size:
1782 1484 1459 644 199 165 157 55
54
BsrDI GCAATG_nn'
Cuts at: 126 2391 3911 126
Size: 2265 1520 2214
Fragments arranged by size:
2265 2214 1520
BsrFI r'CCGG_y
Cuts at: 599 1118 2158 2182 2241 2324 3997 4178 4387
Size: 519 1040 24 59 83 1673 181 209
Cuts at: 4387 599
Size: 2211
Fragments arranged by size:
2211 1673 1040 519 209 181 83 59
24
BssHII G'CGCG_C
Cuts at: 2286 2488 2647 2286
Size: 202 159 5638
Fragments arranged by size:
5638 202 159
BssSI C'ACGA_G
Cuts at: 1579 2533 4270 4628 1579
Size: 954 1737 358 2950
Fragments arranged by size:
2950 1737 954 358
BstAPI GCAn_nnn'nTGC
Cuts at: 285 589 609 1767 3376 285
Size: 304 20 1158 1609 2908
Fragments arranged by size:
2908 1609 1158 304 20
BstBI TT'CG_AA
Cuts at: 4360 4360
Size: 5999
BstEII G'GTnAC_C
Cuts at: 477 498 477
Size: 21 5978
BstXI CCAn_nnnn'nTGG
Cuts at: 144 247 296 144
Size: 103 49 5847
Fragments arranged by size:
5847 103 49
BstYI r'GATC_y
Cuts at: 1 3024 3491 3514 3849 4095 5107 5193 5205
Size: 3023 467 23 335 246 1012 86 12
Cuts at: 5205 5551 1
Size: 346 449
Fragments arranged by size:
3023 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1190 1684 2684 1190
Size: 494 1000 4505
Fragments arranged by size:
4505 1000 494
BtgI C'CryG_G
Cuts at: 107 527 1217 1342 1557 1636 2357 2432 2783
Size: 420 690 125 215 79 721 75 351
Cuts at: 2783 2813 2839 2933 107
Size: 30 26 94 3173
Fragments arranged by size:
3173 721 690 420 351 215 125 94
79 75 30 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2283 3949 4130 4293 2283
Size: 1666 181 163 3989
Fragments arranged by size:
3989 1666 181 163
BtsI GCAGTG_nn'
Cuts at: 279 666 790 1327 1375 1917 3242 279
Size: 387 124 537 48 542 1325 3036
Fragments arranged by size:
3036 1325 542 537 387 124 48
DraI TTT'AAA
Cuts at: 2998 2998
Size: 5999
DraIII CAC_nnn'GTG
Cuts at: 844 1218 1511 1584 844
Size: 374 293 73 5259
Fragments arranged by size:
5259 374 293 73
DrdI GACnn_nn'nnGTC
Cuts at: 1483 3705 4563 1483
Size: 2222 858 2919
Fragments arranged by size:
2919 2222 858
EaeI y'GGCC_r
Cuts at: 131 347 632 798 846 911 959 1199 1241
Size: 216 285 166 48 65 48 240 42
Cuts at: 1241 1395 3584 3758 4149 4176 5521 131
Size: 154 2189 174 391 27 1345 609
Fragments arranged by size:
2189 1345 609 391 285 240 216 174
166 154 65 48 48 42 27
EagI C'GGCC_G
Cuts at: 131 1241 3584 131
Size: 1110 2343 2546
Fragments arranged by size:
2546 2343 1110
EarI CTCTTCn'nnn_
Cuts at: 4022 4232 4022
Size: 210 5789
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1057 2387 4207 4517 4663 5809 1057
Size: 1330 1820 310 146 1146 1247
Fragments arranged by size:
1820 1330 1247 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1697 3011 1697
Size: 1314 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 250 298 397 961 1558 2167 2440 2526
Size: 33 48 99 564 597 609 273 86
Cuts at: 2526 3824 4256 5003 5271 217
Size: 1298 432 747 268 945
Fragments arranged by size:
1298 945 747 609 597 564 432 273
268 99 86 48 33
EcoO109I rG'GnC_Cy
Cuts at: 402 574 682 769 823 990 1004 1176 1901
Size: 172 108 87 54 167 14 172 725
Cuts at: 1901 2411 2680 2907 3138 402
Size: 510 269 227 231 3263
Fragments arranged by size:
3263 725 510 269 231 227 172 172
167 108 87 54 14
EcoRI G'AATT_C
Cuts at: 3003 3003
Size: 5999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2667 2898 4018 2667
Size: 231 1120 4648
Fragments arranged by size:
4648 1120 231
FspI TGC'GCA
Cuts at: 1381 2224 2308 2383 3780 1381
Size: 843 84 75 1397 3600
Fragments arranged by size:
3600 1397 843 84 75
HaeII r_GCGC'y
Cuts at: 213 867 1078 1134 1154 1362 1932 2165 2849
Size: 654 211 56 20 208 570 233 684
Cuts at: 2849 3433 3681 4703 5682 213
Size: 584 248 1022 979 530
Fragments arranged by size:
1022 979 684 654 584 570 530 248
233 211 208 56 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1789 1821 2794 2826 3841 3871 3873 3903 1789
Size: 32 973 32 1015 30 2 30 3885
Fragments arranged by size:
3885 1015 973 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3038 3038
Size: 5999
KasI G'GCGC_C
Cuts at: 209 1074 1358 2845 3429 3677 209
Size: 865 284 1487 584 248 2531
Fragments arranged by size:
2531 1487 865 584 284 248
KpnI G_GTAC'C
Cuts at: 1884 2621 3019 1884
Size: 737 398 4864
Fragments arranged by size:
4864 737 398
MluI A'CGCG_T
Cuts at: 5432 5432
Size: 5999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2617 4670 4854 2495
Size: 122 2053 184 3640
Fragments arranged by size:
3640 2053 184 122
MscI TGG'CCA
Cuts at: 349 634 800 1201 3760 349
Size: 285 166 401 2559 2588
Fragments arranged by size:
2588 2559 401 285 166
MslI CAynn'nnrTG
Cuts at: 2434 3988 4115 2434
Size: 1554 127 4318
Fragments arranged by size:
4318 1554 127
MspA1I CmG'CkG
Cuts at: 228 306 916 1317 1344 1952 2662 2935 3110
Size: 78 610 401 27 608 710 273 175
Cuts at: 3110 3424 3784 4797 5042 228
Size: 314 360 1013 245 1185
Fragments arranged by size:
1185 1013 710 610 608 401 360 314
273 245 175 78 27
NaeI GCC'GGC
Cuts at: 2160 2184 4180 2160
Size: 24 1996 3979
Fragments arranged by size:
3979 1996 24
NarI GG'CG_CC
Cuts at: 210 1075 1359 2846 3430 3678 210
Size: 865 284 1487 584 248 2531
Fragments arranged by size:
2531 1487 865 584 284 248
NcoI C'CATG_G
Cuts at: 107 1557 2357 2783 107
Size: 1450 800 426 3323
Fragments arranged by size:
3323 1450 800 426
NgoMIV G'CCGG_C
Cuts at: 2158 2182 4178 2158
Size: 24 1996 3979
Fragments arranged by size:
3979 1996 24
NotI GC'GGCC_GC
Cuts at: 1241 1241
Size: 5999
NspI r_CATG'y
Cuts at: 738 1765 3052 3552 4459 738
Size: 1027 1287 500 907 2278
Fragments arranged by size:
2278 1287 1027 907 500
PasI CC'CwG_GG
Cuts at: 1905 1905
Size: 5999
PciI A'CATG_T
Cuts at: 4455 4455
Size: 5999
PflMI CCAn_nnn'nTGG
Cuts at: 751 772 844 751
Size: 21 72 5906
Fragments arranged by size:
5906 72 21
PfoI T'CCnGG_A
Cuts at: 314 1424 1727 314
Size: 1110 303 4586
Fragments arranged by size:
4586 1110 303
PmeI GTTT'AAAC
Cuts at: 2998 2998
Size: 5999
PmlI CAC'GTG
Cuts at: 748 1851 2580 748
Size: 1103 729 4167
Fragments arranged by size:
4167 1103 729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 2001 5138 5170 1969
Size: 32 3137 32 2798
Fragments arranged by size:
3137 2798 32 32
PpuMI rG'GwC_Cy
Cuts at: 402 823 1176 402
Size: 421 353 5225
Fragments arranged by size:
5225 421 353
PshAI GACnn'nnGTC
Cuts at: 475 1657 475
Size: 1182 4817
PspOMI G'GGCC_C
Cuts at: 531 1900 2796 2920 531
Size: 1369 896 124 3610
Fragments arranged by size:
3610 1369 896 124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1828 1860 1828
Size: 32 5967
PstI C_TGCA'G
Cuts at: 1256 1568 1772 2171 2264 2504 2588 2859 3046
Size: 312 204 399 93 240 84 271 187
Cuts at: 3046 3731 1256
Size: 685 3524
Fragments arranged by size:
3524 685 399 312 271 240 204 187
93 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5999
PvuII CAG'CTG
Cuts at: 228 306 1952 2662 3424 3784 228
Size: 78 1646 710 762 360 2443
Fragments arranged by size:
2443 1646 762 710 360 78
RsrII CG'GwC_CG
Cuts at: 4194 4194
Size: 5999
SacI G_AGCT'C
Cuts at: 1699 3013 1699
Size: 1314 4685
SacII CC_GC'GG
Cuts at: 1345 2936 1345
Size: 1591 4408
SalI G'TCGA_C
Cuts at: 3036 3036
Size: 5999
SanDI GG'GwC_CC
Cuts at: 1176 1176
Size: 5999
SapI GCTCTTCn'nnn_
Cuts at: 4022 4232 4022
Size: 210 5789
SbfI CC_TGCA'GG
Cuts at: 2504 3046 2504
Size: 542 5457
ScaI AGT'ACT
Cuts at: 5516 5516
Size: 5999
SexAI A'CCwGG_T
Cuts at: 840 840
Size: 5999
SfcI C'TryA_G
Cuts at: 33 223 1252 1564 1768 1916 2167 2260 2500
Size: 190 1029 312 204 148 251 93 240
Cuts at: 2500 2584 2855 3042 3727 4720 4911 33
Size: 84 271 187 685 993 191 1121
Fragments arranged by size:
1121 1029 993 685 312 271 251 240
204 191 190 187 148 93 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 346 761 1240 2928 346
Size: 415 479 1688 3417
Fragments arranged by size:
3417 1688 479 415
SfoI GGC'GCC
Cuts at: 211 1076 1360 2847 3431 3679 211
Size: 865 284 1487 584 248 2531
Fragments arranged by size:
2531 1487 865 584 284 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5999
SgrAI Cr'CCGG_yG
Cuts at: 2158 2158
Size: 5999
SmaI CCC'GGG
Cuts at: 694 1474 1869 1899 2919 3021 694
Size: 780 395 30 1020 102 3672
Fragments arranged by size:
3672 1020 780 395 102 30
SmlI C'TyrA_G
Cuts at: 644 2746 3154 4069 4561 4823 644
Size: 2102 408 915 492 262 1820
Fragments arranged by size:
2102 1820 915 492 408 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5999
SphI G_CATG'C
Cuts at: 738 1765 3052 3552 738
Size: 1027 1287 500 3185
Fragments arranged by size:
3185 1287 1027 500
SrfI GCCC'GGGC
Cuts at: 1899 1899
Size: 5999
StuI AGG'CCT
Cuts at: 340 975 2866 340
Size: 635 1891 3473
Fragments arranged by size:
3473 1891 635
StyI C'CwwG_G
Cuts at: 107 473 759 1557 1992 2357 2642 2675 2783
Size: 366 286 798 435 365 285 33 108
Cuts at: 2783 3133 3487 107
Size: 350 354 2619
Fragments arranged by size:
2619 798 435 366 365 354 350 286
285 108 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 429 2287 3582 3715 4174 4383 429
Size: 1858 1295 133 459 209 2045
Fragments arranged by size:
2045 1858 1295 459 209 133
TatI w'GTAC_w
Cuts at: 5514 5514
Size: 5999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1633 1665 5516 5548 1633
Size: 32 3851 32 2084
Fragments arranged by size:
3851 2084 32 32
Tth111I GACn'n_nGTC
Cuts at: 3796 3796
Size: 5999
XbaI T'CTAG_A
Cuts at: 3030 3030
Size: 5999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2790 3494 2790
Size: 704 5295
XhoI C'TCGA_G
Cuts at: 644 644
Size: 5999
XmaI C'CCGG_G
Cuts at: 692 1472 1867 1897 2917 3019 692
Size: 780 395 30 1020 102 3672
Fragments arranged by size:
3672 1020 780 395 102 30
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI AvrII BaeI BfrBI
BmtI BsgI BsiWI BsmI BsrGI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PsiI PspXI SnaBI SspI
SwaI XmnI ZraI