Restriction map for
pF1KE2133
(Circular) (Six-base) MAPSORT of: pF1KE2133.seq Check: 257 from: 1 to: 6092
pF1KE2133 6092 bp
With 182 enzymes: SgfI *
February 28, 2012 13:55 ..
AccI GT'mk_AC
Cuts at: 3130 3130
Size: 6092
Acc65I G'GTAC_C
Cuts at: 3108 3108
Size: 6092
AclI AA'CG_TT
Cuts at: 5932 5932
Size: 6092
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3917 4349 5096 5364 2414
Size: 1503 432 747 268 3142
Fragments arranged by size:
3142 1503 747 432 268
AflIII A'CryG_T
Cuts at: 3444 4548 5525 3444
Size: 1104 977 4011
Fragments arranged by size:
4011 1104 977
AgeI A'CCGG_T
Cuts at: 4480 4480
Size: 6092
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1491 1523 1491
Size: 32 6060
AlwNI CAG_nnn'CTG
Cuts at: 3397 4964 3397
Size: 1567 4525
ApaLI G'TGCA_C
Cuts at: 2148 4862 2148
Size: 2714 3378
ApoI r'AATT_y
Cuts at: 1932 2233 2307 2379 2506 2515 3096 5445 5701
Size: 301 74 72 127 9 581 2349 256
Cuts at: 5701 1932
Size: 2323
Fragments arranged by size:
2349 2323 581 301 256 127 74 72
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 6092
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6092
AvaI C'yCGr_G
Cuts at: 295 982 2606 3112 295
Size: 687 1624 506 3275
Fragments arranged by size:
3275 1624 687 506
BamHI G'GATC_C
Cuts at: 3117 3117
Size: 6092
BanI G'GyrC_C
Cuts at: 3108 3522 3770 3805 3108
Size: 414 248 35 5395
Fragments arranged by size:
5395 414 248 35
BanII G_rGCy'C
Cuts at: 322 956 3106 4136 322
Size: 634 2150 1030 2278
Fragments arranged by size:
2278 2150 1030 634
BbeI G_GCGC'C
Cuts at: 3526 3774 3526
Size: 248 5844
BbsI GAAGACnn'nnnn_
Cuts at: 296 621 296
Size: 325 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1424 1458 90
Size: 34 1300 34 4724
Fragments arranged by size:
4724 1300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1078 1300 1468 3990 4751 6092 1078
Size: 222 168 2522 761 1341 1078
Fragments arranged by size:
2522 1341 1078 761 222 168
BclI T'GATC_A
Cuts at: 203 3612 203
Size: 3409 2683
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 6092
BglII A'GATC_T
Cuts at: 192 849 2466 3607 5644 192
Size: 657 1617 1141 2037 640
Fragments arranged by size:
2037 1617 1141 657 640
BlpI GC'TnA_GC
Cuts at: 3204 3204
Size: 6092
Bme1580I G_kGCm'C
Cuts at: 2152 3717 3810 4866 2152
Size: 1565 93 1056 3378
Fragments arranged by size:
3378 1565 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 826 1109 3304 3385 3720 5864 826
Size: 283 2195 81 335 2144 1054
Fragments arranged by size:
2195 2144 1054 335 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1622 2095 4459 1622
Size: 473 2364 3255
Fragments arranged by size:
3255 2364 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1685 542
Size: 1143 4949
Bpu10I CC'TnA_GC
Cuts at: 321 3071 321
Size: 2750 3342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1409 2077 2880 3268 4147 4639 4937 1409
Size: 668 803 388 879 492 298 2564
Fragments arranged by size:
2564 879 803 668 492 388 298
BsaI GGTCTCn'nnnn_
Cuts at: 303 2531 5796 303
Size: 2228 3265 599
Fragments arranged by size:
3265 2228 599
BsaAI yAC'GTr
Cuts at: 1253 3445 1253
Size: 2192 3900
BsaBI GATnn'nnATC
Cuts at: 6 854 3606 5643 6
Size: 848 2752 2037 455
Fragments arranged by size:
2752 2037 848 455
BsaHI Gr'CG_yC
Cuts at: 647 1456 3523 3771 4473 5969 647
Size: 809 2067 248 702 1496 770
Fragments arranged by size:
2067 1496 809 770 702 248
BsaWI w'CCGG_w
Cuts at: 1081 2784 3281 3802 4480 4754 4901 5312 1081
Size: 1703 497 521 678 274 147 411 1861
Fragments arranged by size:
1861 1703 678 521 497 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 491 854 1000 1753 1848 2452 491
Size: 363 146 753 95 604 4131
Fragments arranged by size:
4131 753 604 363 146 95
BseYI C'CCAG_C
Cuts at: 2105 2109 2139 2882 3517 4275 4852 5348 2105
Size: 4 30 743 635 758 577 496 2849
Fragments arranged by size:
2849 758 743 635 577 496 30 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1512 1512
Size: 6092
BsiEI CG_ry'CG
Cuts at: 104 686 3634 3680 4888 104
Size: 582 2948 46 1208 1308
Fragments arranged by size:
2948 1308 1208 582 46
BsiHKAI G_wGCw'C
Cuts at: 322 1433 2152 3106 3884 4074 4866 322
Size: 1111 719 954 778 190 792 1548
Fragments arranged by size:
1548 1111 954 792 778 719 190
BsmI GAATG_Cn'
Cuts at: 832 2780 832
Size: 1948 4144
BsmBI CGTCTCn'nnnn_
Cuts at: 1675 1675
Size: 6092
Bsp1286I G_dGCh'C
Cuts at: 322 956 1433 2152 3106 3717 3810 3884 4074
Size: 634 477 719 954 611 93 74 190
Cuts at: 4074 4136 4866 322
Size: 62 730 1548
Fragments arranged by size:
1548 954 730 719 634 611 477 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5312 5312
Size: 6092
BspHI T'CATG_A
Cuts at: 771 2615 5268 771
Size: 1844 2653 1595
Fragments arranged by size:
2653 1844 1595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2144 3142 3356 3658 4039 2144
Size: 998 214 302 381 4197
Fragments arranged by size:
4197 998 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4384 4438 5922 6087 50
Size: 4334 54 1484 165 55
Fragments arranged by size:
4334 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 170 874 2652 4004 170
Size: 704 1778 1352 2258
Fragments arranged by size:
2258 1778 1352 704
BsrFI r'CCGG_y
Cuts at: 111 1852 4090 4271 4480 111
Size: 1741 2238 181 209 1723
Fragments arranged by size:
2238 1741 1723 209 181
BssHII G'CGCG_C
Cuts at: 121 121
Size: 6092
BssSI C'ACGA_G
Cuts at: 2119 4363 4721 2119
Size: 2244 358 3490
Fragments arranged by size:
3490 2244 358
BstAPI GCAn_nnn'nTGC
Cuts at: 827 3469 827
Size: 2642 3450
BstBI TT'CG_AA
Cuts at: 4453 4453
Size: 6092
BstEII G'GTnAC_C
Cuts at: 1131 1848 1131
Size: 717 5375
BstXI CCAn_nnnn'nTGG
Cuts at: 2502 2502
Size: 6092
BstYI r'GATC_y
Cuts at: 1 192 849 2466 3117 3584 3607 3942 4188
Size: 191 657 1617 651 467 23 335 246
Cuts at: 4188 5200 5286 5298 5644 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1617 1012 657 651 467 449 346 335
246 191 86 23 12
BtgI C'CryG_G
Cuts at: 269 488 942 2568 269
Size: 219 454 1626 3793
Fragments arranged by size:
3793 1626 454 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 4042 4223 4386 2980
Size: 1062 181 163 4686
Fragments arranged by size:
4686 1062 181 163
BtsI GCAGTG_nn'
Cuts at: 376 3335 376
Size: 2959 3133
DraI TTT'AAA
Cuts at: 1227 1694 2826 3091 1227
Size: 467 1132 265 4228
Fragments arranged by size:
4228 1132 467 265
DraIII CAC_nnn'GTG
Cuts at: 2761 2761
Size: 6092
DrdI GACnn_nn'nnGTC
Cuts at: 652 3798 4656 652
Size: 3146 858 2088
Fragments arranged by size:
3146 2088 858
EaeI y'GGCC_r
Cuts at: 132 2543 2905 2985 3677 3851 4242 4269 5614
Size: 2411 362 80 692 174 391 27 1345
Cuts at: 5614 132
Size: 610
Fragments arranged by size:
2411 1345 692 610 391 362 174 80
27
EagI C'GGCC_G
Cuts at: 3677 3677
Size: 6092
EarI CTCTTCn'nnn_
Cuts at: 1107 1196 1529 1771 2429 4115 4325 1107
Size: 89 333 242 658 1686 210 2874
Fragments arranged by size:
2874 1686 658 333 242 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4300 4610 4756 5902 4300
Size: 310 146 1146 4490
Fragments arranged by size:
4490 1146 310 146
EcoICRI GAG'CTC
Cuts at: 320 3104 320
Size: 2784 3308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1685 2414 3917 4349 5096 5364 542
Size: 1143 729 1503 432 747 268 1270
Fragments arranged by size:
1503 1270 1143 747 729 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1705 3231 1705
Size: 1526 4566
EcoRI G'AATT_C
Cuts at: 2379 3096 2379
Size: 717 5375
EcoRV GAT'ATC
Cuts at: 2613 2613
Size: 6092
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 635 2488 4111 635
Size: 1853 1623 2616
Fragments arranged by size:
2616 1853 1623
FspI TGC'GCA
Cuts at: 3873 3873
Size: 6092
HaeII r_GCGC'y
Cuts at: 3526 3774 4796 5775 3526
Size: 248 1022 979 3843
Fragments arranged by size:
3843 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 938 970 1248 1280 1717 1749 2006 2038 2347
Size: 32 278 32 437 32 257 32 309
Cuts at: 2347 2379 2707 2739 3934 3964 3966 3996 938
Size: 32 328 32 1195 30 2 30 3034
Fragments arranged by size:
3034 1195 437 328 309 278 257 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3131 3131
Size: 6092
KasI G'GCGC_C
Cuts at: 3522 3770 3522
Size: 248 5844
KpnI G_GTAC'C
Cuts at: 3112 3112
Size: 6092
MfeI C'AATT_G
Cuts at: 1748 1748
Size: 6092
MluI A'CGCG_T
Cuts at: 5525 5525
Size: 6092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2651 4763 4947 2651
Size: 2112 184 3796
Fragments arranged by size:
3796 2112 184
MscI TGG'CCA
Cuts at: 3853 3853
Size: 6092
MslI CAynn'nnrTG
Cuts at: 2869 4081 4208 2869
Size: 1212 127 4753
Fragments arranged by size:
4753 1212 127
MspA1I CmG'CkG
Cuts at: 138 144 346 742 2109 2946 3203 3517 3877
Size: 6 202 396 1367 837 257 314 360
Cuts at: 3877 4890 5135 138
Size: 1013 245 1095
Fragments arranged by size:
1367 1095 1013 837 396 360 314 257
245 202 6
NaeI GCC'GGC
Cuts at: 113 4273 113
Size: 4160 1932
NarI GG'CG_CC
Cuts at: 3523 3771 3523
Size: 248 5844
NcoI C'CATG_G
Cuts at: 269 488 942 269
Size: 219 454 5419
Fragments arranged by size:
5419 454 219
NdeI CA'TA_TG
Cuts at: 2727 2727
Size: 6092
NgoMIV G'CCGG_C
Cuts at: 111 4271 111
Size: 4160 1932
NspI r_CATG'y
Cuts at: 2319 3145 3645 4552 2319
Size: 826 500 907 3859
Fragments arranged by size:
3859 907 826 500
PasI CC'CwG_GG
Cuts at: 400 400
Size: 6092
PciI A'CATG_T
Cuts at: 4548 4548
Size: 6092
PmeI GTTT'AAAC
Cuts at: 3091 3091
Size: 6092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 5231
Size: 32 6060
PsiI TTA'TAA
Cuts at: 1156 2869 1156
Size: 1713 4379
PspXI vC'TCGA_Gb
Cuts at: 982 982
Size: 6092
PstI C_TGCA'G
Cuts at: 3139 3824 3139
Size: 685 5407
PvuI CG_AT'CG
Cuts at: 104 686 104
Size: 582 5510
PvuII CAG'CTG
Cuts at: 138 742 2109 2946 3517 3877 138
Size: 604 1367 837 571 360 2353
Fragments arranged by size:
2353 1367 837 604 571 360
RsrII CG'GwC_CG
Cuts at: 4287 4287
Size: 6092
SacI G_AGCT'C
Cuts at: 322 3106 322
Size: 2784 3308
SalI G'TCGA_C
Cuts at: 3129 3129
Size: 6092
SapI GCTCTTCn'nnn_
Cuts at: 4115 4325 4115
Size: 210 5882
SbfI CC_TGCA'GG
Cuts at: 3139 3139
Size: 6092
ScaI AGT'ACT
Cuts at: 1605 1873 5609 1605
Size: 268 3736 2088
Fragments arranged by size:
3736 2088 268
SfcI C'TryA_G
Cuts at: 33 1834 2849 3135 3820 4813 5004 33
Size: 1801 1015 286 685 993 191 1121
Fragments arranged by size:
1801 1121 1015 993 685 286 191
SfoI GGC'GCC
Cuts at: 3524 3772 3524
Size: 248 5844
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6092
SmaI CCC'GGG
Cuts at: 3114 3114
Size: 6092
SmlI C'TyrA_G
Cuts at: 982 1424 2092 2606 2895 3247 4162 4654 4916
Size: 442 668 514 289 352 915 492 262
Cuts at: 4916 982
Size: 2158
Fragments arranged by size:
2158 915 668 514 492 442 352 289
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6092
SphI G_CATG'C
Cuts at: 2319 3145 3645 2319
Size: 826 500 4766
Fragments arranged by size:
4766 826 500
SspI AAT'ATT
Cuts at: 569 2127 2735 569
Size: 1558 608 3926
Fragments arranged by size:
3926 1558 608
StyI C'CwwG_G
Cuts at: 269 488 942 1665 3226 3580 269
Size: 219 454 723 1561 354 2781
Fragments arranged by size:
2781 1561 723 454 354 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 684 1581 2581 3675 3808 4267 4476 684
Size: 897 1000 1094 133 459 209 2300
Fragments arranged by size:
2300 1094 1000 897 459 209 133
TatI w'GTAC_w
Cuts at: 1603 1802 1871 5607 1603
Size: 199 69 3736 2088
Fragments arranged by size:
3736 2088 199 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 781 813 1577 1609 5609 5641 781
Size: 32 764 32 4000 32 1232
Fragments arranged by size:
4000 1232 764 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3889 3889
Size: 6092
XbaI T'CTAG_A
Cuts at: 3123 3123
Size: 6092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 813 1487 1493 3587 114
Size: 699 674 6 2094 2619
Fragments arranged by size:
2619 2094 699 674 6
XhoI C'TCGA_G
Cuts at: 982 2606 982
Size: 1624 4468
XmaI C'CCGG_G
Cuts at: 3112 3112
Size: 6092
XmnI GAAnn'nnTTC
Cuts at: 2234 2468 2234
Size: 234 5858
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsaXI
BsiWI BsrGI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI