Restriction map for
pF1KE2129
(Circular) (Six-base) MAPSORT of: pF1KE2129.seq Check: 624 from: 1 to: 5906
pF1KE2129 5906 bp
With 182 enzymes: SgfI *
March 14, 2016 17:24 ..
AccI GT'mk_AC
Cuts at: 2944 2944
Size: 5906
Acc65I G'GTAC_C
Cuts at: 2922 2922
Size: 5906
AclI AA'CG_TT
Cuts at: 482 5746 482
Size: 5264 642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1145 1913 2186 2461 2779 3731 4163 4910
Size: 456 768 273 275 318 952 432 747
Cuts at: 4910 5178 689
Size: 268 1417
Fragments arranged by size:
1417 952 768 747 456 432 318 275
273 268
AflIII A'CryG_T
Cuts at: 365 3258 4362 5339 365
Size: 2893 1104 977 932
Fragments arranged by size:
2893 1104 977 932
AgeI A'CCGG_T
Cuts at: 4294 4294
Size: 5906
AhdI GACnn_n'nnGTC
Cuts at: 1477 1477
Size: 5906
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 129 161 129
Size: 32 5874
AlwNI CAG_nnn'CTG
Cuts at: 3211 4778 3211
Size: 1567 4339
ApaI G_GGCC'C
Cuts at: 134 1660 134
Size: 1526 4380
ApaLI G'TGCA_C
Cuts at: 4676 4676
Size: 5906
ApoI r'AATT_y
Cuts at: 1234 2200 2335 2540 2910 5259 5515 1234
Size: 966 135 205 370 2349 256 1625
Fragments arranged by size:
2349 1625 966 370 256 205 135
AseI AT'TA_AT
Cuts at: 20 929 20
Size: 909 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5906
AvaI C'yCGr_G
Cuts at: 152 584 2926 152
Size: 432 2342 3132
Fragments arranged by size:
3132 2342 432
BamHI G'GATC_C
Cuts at: 2931 2931
Size: 5906
BanI G'GyrC_C
Cuts at: 2406 2922 3336 3584 3619 2406
Size: 516 414 248 35 4693
Fragments arranged by size:
4693 516 414 248 35
BanII G_rGCy'C
Cuts at: 121 134 1660 2626 2920 3950 121
Size: 13 1526 966 294 1030 2077
Fragments arranged by size:
2077 1526 1030 966 294 13
BbeI G_GCGC'C
Cuts at: 3340 3588 3340
Size: 248 5658
BbsI GAAGACnn'nnnn_
Cuts at: 233 2564 2834 233
Size: 2331 270 3305
Fragments arranged by size:
3305 2331 270
BciVI GTATCCnnnnn_n'
Cuts at: 3804 4565 5906 3804
Size: 761 1341 3804
Fragments arranged by size:
3804 1341 761
BclI T'GATC_A
Cuts at: 1176 2285 3426 1176
Size: 1109 1141 3656
Fragments arranged by size:
3656 1141 1109
BglII A'GATC_T
Cuts at: 919 3421 5458 919
Size: 2502 2037 1367
Fragments arranged by size:
2502 2037 1367
BlpI GC'TnA_GC
Cuts at: 3018 3018
Size: 5906
Bme1580I G_kGCm'C
Cuts at: 134 1660 2600 3531 3624 4680 134
Size: 1526 940 931 93 1056 1360
Fragments arranged by size:
1526 1360 1056 940 931 93
BmrI ACTGGGnnnn_n'
Cuts at: 436 3118 3199 3534 5678 436
Size: 2682 81 335 2144 664
Fragments arranged by size:
2682 2144 664 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4273 4273
Size: 5906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 900 1150 694
Size: 206 250 5450
Fragments arranged by size:
5450 250 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 2766 3082 3961 4453 4751 550
Size: 2216 316 879 492 298 1705
Fragments arranged by size:
2216 1705 879 492 316 298
BsaI GGTCTCn'nnnn_
Cuts at: 5610 5610
Size: 5906
BsaAI yAC'GTr
Cuts at: 3259 3259
Size: 5906
BsaBI GATnn'nnATC
Cuts at: 6 2636 3420 5457 6
Size: 2630 784 2037 455
Fragments arranged by size:
2630 2037 784 455
BsaHI Gr'CG_yC
Cuts at: 3337 3585 4287 5783 3337
Size: 248 702 1496 3460
Fragments arranged by size:
3460 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3095 3616 4294 4568 4715 5126 3095
Size: 521 678 274 147 411 3875
Fragments arranged by size:
3875 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 129 159 1435 1465 1817 1847 129
Size: 30 1276 30 352 30 4188
Fragments arranged by size:
4188 1276 352 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 2386 415
Size: 1971 3935
BseYI C'CCAG_C
Cuts at: 219 3331 4089 4666 5162 219
Size: 3112 758 577 496 963
Fragments arranged by size:
3112 963 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1134 1134
Size: 5906
BsiEI CG_ry'CG
Cuts at: 104 1185 3448 3494 4702 104
Size: 1081 2263 46 1208 1308
Fragments arranged by size:
2263 1308 1208 1081 46
BsiHKAI G_wGCw'C
Cuts at: 2626 2920 3698 3888 4680 2626
Size: 294 778 190 792 3852
Fragments arranged by size:
3852 792 778 294 190
BsmI GAATG_Cn'
Cuts at: 472 516 1491 472
Size: 44 975 4887
Fragments arranged by size:
4887 975 44
Bsp1286I G_dGCh'C
Cuts at: 121 134 1660 2600 2626 2920 3531 3624 3698
Size: 13 1526 940 26 294 611 93 74
Cuts at: 3698 3888 3950 4680 121
Size: 190 62 730 1347
Fragments arranged by size:
1526 1347 940 730 611 294 190 93
74 62 26 13
BspEI T'CCGG_A
Cuts at: 5126 5126
Size: 5906
BspHI T'CATG_A
Cuts at: 852 989 1427 1481 2553 5082 852
Size: 137 438 54 1072 2529 1676
Fragments arranged by size:
2529 1676 1072 438 137 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2956 3170 3472 3853 2956
Size: 214 302 381 5009
Fragments arranged by size:
5009 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4198 4252 5736 5901 50
Size: 4148 54 1484 165 55
Fragments arranged by size:
4148 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 879 1017 1730 3818 879
Size: 138 713 2088 2967
Fragments arranged by size:
2967 2088 713 138
BsrFI r'CCGG_y
Cuts at: 305 3904 4085 4294 305
Size: 3599 181 209 1917
Fragments arranged by size:
3599 1917 209 181
BssSI C'ACGA_G
Cuts at: 4177 4535 4177
Size: 358 5548
BstAPI GCAn_nnn'nTGC
Cuts at: 878 3283 878
Size: 2405 3501
BstBI TT'CG_AA
Cuts at: 4267 4267
Size: 5906
BstXI CCAn_nnnn'nTGG
Cuts at: 1467 1467
Size: 5906
BstYI r'GATC_y
Cuts at: 1 283 919 981 2931 3398 3421 3756 4002
Size: 282 636 62 1950 467 23 335 246
Cuts at: 4002 5014 5100 5112 5458 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1950 1012 636 467 449 346 335 282
246 86 62 23 12
BtgI C'CryG_G
Cuts at: 2870 2870
Size: 5906
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3856 4037 4200 3856
Size: 181 163 5562
Fragments arranged by size:
5562 181 163
BtsI GCAGTG_nn'
Cuts at: 533 789 1084 3149 533
Size: 256 295 2065 3290
Fragments arranged by size:
3290 2065 295 256
DraI TTT'AAA
Cuts at: 1205 2905 1205
Size: 1700 4206
DrdI GACnn_nn'nnGTC
Cuts at: 3612 4470 3612
Size: 858 5048
EaeI y'GGCC_r
Cuts at: 530 1028 1850 3491 3665 4056 4083 5428 530
Size: 498 822 1641 174 391 27 1345 1008
Fragments arranged by size:
1641 1345 1008 822 498 391 174 27
EagI C'GGCC_G
Cuts at: 3491 3491
Size: 5906
EarI CTCTTCn'nnn_
Cuts at: 2117 3929 4139 2117
Size: 1812 210 3884
Fragments arranged by size:
3884 1812 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4114 4424 4570 5716 4114
Size: 310 146 1146 4304
Fragments arranged by size:
4304 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2624 2918 2624
Size: 294 5612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 694 900 1145 1150 1913 2186 2461 2779
Size: 5 206 245 5 763 273 275 318
Cuts at: 2779 3731 4163 4910 5178 689
Size: 952 432 747 268 1417
Fragments arranged by size:
1417 952 763 747 432 318 275 273
268 245 206 5 5
EcoNI CCTnn'n_nnAGG
Cuts at: 826 826
Size: 5906
EcoO109I rG'GnC_Cy
Cuts at: 131 1656 1657 1866 3045 131
Size: 1525 1 209 1179 2992
Fragments arranged by size:
2992 1525 1179 209 1
EcoRI G'AATT_C
Cuts at: 2910 2910
Size: 5906
EcoRV GAT'ATC
Cuts at: 1982 1982
Size: 5906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 974 3925 974
Size: 2951 2955
FspI TGC'GCA
Cuts at: 3687 3687
Size: 5906
HaeII r_GCGC'y
Cuts at: 161 3340 3588 4610 5589 161
Size: 3179 248 1022 979 478
Fragments arranged by size:
3179 1022 979 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1161 1193 1463 1495 2851 2883 3748 3778 3780
Size: 32 270 32 1356 32 865 30 2
Cuts at: 3780 3810 1161
Size: 30 3257
Fragments arranged by size:
3257 1356 865 270 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 486 2945 486
Size: 2459 3447
HindIII A'AGCT_T
Cuts at: 1962 2443 1962
Size: 481 5425
HpaI GTT'AAC
Cuts at: 486 486
Size: 5906
KasI G'GCGC_C
Cuts at: 3336 3584 3336
Size: 248 5658
KpnI G_GTAC'C
Cuts at: 2926 2926
Size: 5906
MfeI C'AATT_G
Cuts at: 1907 1907
Size: 5906
MluI A'CGCG_T
Cuts at: 5339 5339
Size: 5906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1077 4577 4761 1077
Size: 3500 184 2222
Fragments arranged by size:
3500 2222 184
MscI TGG'CCA
Cuts at: 532 1030 1852 3667 532
Size: 498 822 1815 2771
Fragments arranged by size:
2771 1815 822 498
MslI CAynn'nnrTG
Cuts at: 442 2035 3895 4022 442
Size: 1593 1860 127 2326
Fragments arranged by size:
2326 1860 1593 127
MspA1I CmG'CkG
Cuts at: 172 249 424 1492 1554 3017 3331 3691 4704
Size: 77 175 1068 62 1463 314 360 1013
Cuts at: 4704 4949 172
Size: 245 1129
Fragments arranged by size:
1463 1129 1068 1013 360 314 245 175
77 62
NaeI GCC'GGC
Cuts at: 4087 4087
Size: 5906
NarI GG'CG_CC
Cuts at: 3337 3585 3337
Size: 248 5658
NcoI C'CATG_G
Cuts at: 2870 2870
Size: 5906
NgoMIV G'CCGG_C
Cuts at: 4085 4085
Size: 5906
NspI r_CATG'y
Cuts at: 369 1692 1759 2959 3459 4366 369
Size: 1323 67 1200 500 907 1909
Fragments arranged by size:
1909 1323 1200 907 500 67
PciI A'CATG_T
Cuts at: 365 4362 365
Size: 3997 1909
PflMI CCAn_nnn'nTGG
Cuts at: 2354 2354
Size: 5906
PfoI T'CCnGG_A
Cuts at: 2300 2492 2300
Size: 192 5714
PmeI GTTT'AAAC
Cuts at: 2905 2905
Size: 5906
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 129 161 548 580 1030 1062 5045 5077 129
Size: 32 387 32 450 32 3983 32 958
Fragments arranged by size:
3983 958 450 387 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1866 1866
Size: 5906
PspOMI G'GGCC_C
Cuts at: 130 1656 130
Size: 1526 4380
PstI C_TGCA'G
Cuts at: 1979 2714 2953 3638 1979
Size: 735 239 685 4247
Fragments arranged by size:
4247 735 685 239
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5906
PvuII CAG'CTG
Cuts at: 424 1492 1554 3331 3691 424
Size: 1068 62 1777 360 2639
Fragments arranged by size:
2639 1777 1068 360 62
RsrII CG'GwC_CG
Cuts at: 4101 4101
Size: 5906
SacI G_AGCT'C
Cuts at: 2626 2920 2626
Size: 294 5612
SalI G'TCGA_C
Cuts at: 2943 2943
Size: 5906
SanDI GG'GwC_CC
Cuts at: 1866 1866
Size: 5906
SapI GCTCTTCn'nnn_
Cuts at: 3929 4139 3929
Size: 210 5696
SbfI CC_TGCA'GG
Cuts at: 2953 2953
Size: 5906
ScaI AGT'ACT
Cuts at: 5423 5423
Size: 5906
SfcI C'TryA_G
Cuts at: 33 395 517 1913 1975 2710 2949 3634 4627
Size: 362 122 1396 62 735 239 685 993
Cuts at: 4627 4818 33
Size: 191 1121
Fragments arranged by size:
1396 1121 993 735 685 362 239 191
122 62
SfoI GGC'GCC
Cuts at: 3338 3586 3338
Size: 248 5658
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5906
SgrAI Cr'CCGG_yG
Cuts at: 305 305
Size: 5906
SmaI CCC'GGG
Cuts at: 154 2928 154
Size: 2774 3132
SmlI C'TyrA_G
Cuts at: 565 584 2781 3061 3976 4468 4730 565
Size: 19 2197 280 915 492 262 1741
Fragments arranged by size:
2197 1741 915 492 280 262 19
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5906
SphI G_CATG'C
Cuts at: 1759 2959 3459 1759
Size: 1200 500 4206
Fragments arranged by size:
4206 1200 500
SspI AAT'ATT
Cuts at: 1035 1669 1035
Size: 634 5272
StuI AGG'CCT
Cuts at: 944 944
Size: 5906
StyI C'CwwG_G
Cuts at: 1661 2870 3040 3394 1661
Size: 1209 170 354 4173
Fragments arranged by size:
4173 1209 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3489 3622 4081 4290 3489
Size: 133 459 209 5105
Fragments arranged by size:
5105 459 209 133
TatI w'GTAC_w
Cuts at: 2706 5421 2706
Size: 2715 3191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1435 1467 1817 1849 5423 5455 1435
Size: 32 350 32 3574 32 1886
Fragments arranged by size:
3574 1886 350 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3703 3703
Size: 5906
XbaI T'CTAG_A
Cuts at: 2937 2937
Size: 5906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 3401 540
Size: 2861 3045
XhoI C'TCGA_G
Cuts at: 584 584
Size: 5906
XmaI C'CCGG_G
Cuts at: 152 2926 152
Size: 2774 3132
XmnI GAAnn'nnTTC
Cuts at: 560 644 1045 2633 560
Size: 84 401 1588 3833
Fragments arranged by size:
3833 1588 401 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SnaBI SrfI SwaI ZraI