Restriction map for
pF1KE2112
(Circular) (Six-base) MAPSORT of: pF1KE2112.seq Check: 7619 from: 1 to: 5876
pF1KE2112 5876 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AccI GT'mk_AC
Cuts at: 2702 2914 2702
Size: 212 5664
Acc65I G'GTAC_C
Cuts at: 2892 2892
Size: 5876
AclI AA'CG_TT
Cuts at: 5716 5716
Size: 5876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 933 1590 1786 1961 2234 2512 3701 4133 4880
Size: 657 196 175 273 278 1189 432 747
Cuts at: 4880 5148 933
Size: 268 1661
Fragments arranged by size:
1661 1189 747 657 432 278 273 268
196 175
AfeI AGC'GCT
Cuts at: 451 451
Size: 5876
AflIII A'CryG_T
Cuts at: 2644 3228 4332 5309 2644
Size: 584 1104 977 3211
Fragments arranged by size:
3211 1104 977 584
AgeI A'CCGG_T
Cuts at: 4264 4264
Size: 5876
AlwNI CAG_nnn'CTG
Cuts at: 750 1585 3181 4748 750
Size: 835 1596 1567 1878
Fragments arranged by size:
1878 1596 1567 835
ApaI G_GGCC'C
Cuts at: 400 400
Size: 5876
ApaLI G'TGCA_C
Cuts at: 853 4646 853
Size: 3793 2083
ApoI r'AATT_y
Cuts at: 1457 2248 2880 5229 5485 1457
Size: 791 632 2349 256 1848
Fragments arranged by size:
2349 1848 791 632 256
AseI AT'TA_AT
Cuts at: 20 2336 20
Size: 2316 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5876
AvaI C'yCGr_G
Cuts at: 963 2896 963
Size: 1933 3943
AvrII C'CTAG_G
Cuts at: 1708 1708
Size: 5876
BamHI G'GATC_C
Cuts at: 2901 2901
Size: 5876
BanI G'GyrC_C
Cuts at: 159 261 380 424 874 2892 3306 3554 3589
Size: 102 119 44 450 2018 414 248 35
Cuts at: 3589 159
Size: 2446
Fragments arranged by size:
2446 2018 450 414 248 119 102 44
35
BanII G_rGCy'C
Cuts at: 400 736 1049 1694 2890 3920 400
Size: 336 313 645 1196 1030 2356
Fragments arranged by size:
2356 1196 1030 645 336 313
BbeI G_GCGC'C
Cuts at: 163 265 384 3310 3558 163
Size: 102 119 2926 248 2481
Fragments arranged by size:
2926 2481 248 119 102
BbsI GAAGACnn'nnnn_
Cuts at: 182 2792 182
Size: 2610 3266
BciVI GTATCCnnnnn_n'
Cuts at: 2260 3774 4535 5876 2260
Size: 1514 761 1341 2260
Fragments arranged by size:
2260 1514 1341 761
BclI T'GATC_A
Cuts at: 2241 3396 2241
Size: 1155 4721
BglI GCCn_nnn'nGGC
Cuts at: 221 997 1069 221
Size: 776 72 5028
Fragments arranged by size:
5028 776 72
BglII A'GATC_T
Cuts at: 1194 1601 2027 3391 5428 1194
Size: 407 426 1364 2037 1642
Fragments arranged by size:
2037 1642 1364 426 407
BlpI GC'TnA_GC
Cuts at: 1048 1969 2988 1048
Size: 921 1019 3936
Fragments arranged by size:
3936 1019 921
Bme1580I G_kGCm'C
Cuts at: 400 857 3501 3594 4650 400
Size: 457 2644 93 1056 1626
Fragments arranged by size:
2644 1626 1056 457 93
BmrI ACTGGGnnnn_n'
Cuts at: 478 1617 3088 3169 3504 5648 478
Size: 1139 1471 81 335 2144 706
Fragments arranged by size:
2144 1471 1139 706 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 1356 4243 273
Size: 1083 2887 1906
Fragments arranged by size:
2887 1906 1083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 268 523 607 1237 2745 246
Size: 22 255 84 630 1508 3377
Fragments arranged by size:
3377 1508 630 255 84 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 2580 3052 3931 4423 4721 592
Size: 1988 472 879 492 298 1747
Fragments arranged by size:
1988 1747 879 492 472 298
BsaI GGTCTCn'nnnn_
Cuts at: 5580 5580
Size: 5876
BsaAI yAC'GTr
Cuts at: 669 3229 669
Size: 2560 3316
BsaBI GATnn'nnATC
Cuts at: 6 3390 5427 6
Size: 3384 2037 455
Fragments arranged by size:
3384 2037 455
BsaHI Gr'CG_yC
Cuts at: 160 233 262 381 461 638 3307 3555 4257
Size: 73 29 119 80 177 2669 248 702
Cuts at: 4257 5753 160
Size: 1496 283
Fragments arranged by size:
2669 1496 702 283 248 177 119 80
73 29
BsaWI w'CCGG_w
Cuts at: 3065 3586 4264 4538 4685 5096 3065
Size: 521 678 274 147 411 3845
Fragments arranged by size:
3845 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2421 2451 2421
Size: 30 5846
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 184 457 460 520 181
Size: 3 273 3 60 5537
Fragments arranged by size:
5537 273 60 3 3
BseYI C'CCAG_C
Cuts at: 1449 2851 3301 4059 4636 5132 1449
Size: 1402 450 758 577 496 2193
Fragments arranged by size:
2193 1402 758 577 496 450
BsiEI CG_ry'CG
Cuts at: 104 892 3418 3464 4672 104
Size: 788 2526 46 1208 1308
Fragments arranged by size:
2526 1308 1208 788 46
BsiHKAI G_wGCw'C
Cuts at: 584 857 1049 1694 1970 2234 2890 3668 3858
Size: 273 192 645 276 264 656 778 190
Cuts at: 3858 4650 584
Size: 792 1810
Fragments arranged by size:
1810 792 778 656 645 276 273 264
192 190
BsmI GAATG_Cn'
Cuts at: 2627 2627
Size: 5876
BsmBI CGTCTCn'nnnn_
Cuts at: 342 727 342
Size: 385 5491
Bsp1286I G_dGCh'C
Cuts at: 400 584 736 857 1049 1694 1970 2234 2890
Size: 184 152 121 192 645 276 264 656
Cuts at: 2890 3501 3594 3668 3858 3920 4650 400
Size: 611 93 74 190 62 730 1626
Fragments arranged by size:
1626 730 656 645 611 276 264 192
190 184 152 121 93 74 62
BspEI T'CCGG_A
Cuts at: 5096 5096
Size: 5876
BspHI T'CATG_A
Cuts at: 5052 5052
Size: 5876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2926 3140 3442 3823 2926
Size: 214 302 381 4979
Fragments arranged by size:
4979 381 302 214
BsrBI CCG'CTC
Cuts at: 50 888 4168 4222 5706 5871 50
Size: 838 3280 54 1484 165 55
Fragments arranged by size:
3280 1484 838 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1126 1766 3788 1126
Size: 640 2022 3214
Fragments arranged by size:
3214 2022 640
BsrFI r'CCGG_y
Cuts at: 377 3874 4055 4264 377
Size: 3497 181 209 1989
Fragments arranged by size:
3497 1989 209 181
BssHII G'CGCG_C
Cuts at: 625 625
Size: 5876
BssSI C'ACGA_G
Cuts at: 4147 4505 4147
Size: 358 5518
BstAPI GCAn_nnn'nTGC
Cuts at: 750 861 3253 750
Size: 111 2392 3373
Fragments arranged by size:
3373 2392 111
BstBI TT'CG_AA
Cuts at: 1550 4237 1550
Size: 2687 3189
BstEII G'GTnAC_C
Cuts at: 147 591 147
Size: 444 5432
BstXI CCAn_nnnn'nTGG
Cuts at: 822 822
Size: 5876
BstYI r'GATC_y
Cuts at: 1 1149 1194 1601 2027 2901 3368 3391 3726
Size: 1148 45 407 426 874 467 23 335
Cuts at: 3726 3972 4984 5070 5082 5428 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1148 1012 874 467 449 426 407 346
335 246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1348 1348
Size: 5876
BtgI C'CryG_G
Cuts at: 309 326 420 309
Size: 17 94 5765
Fragments arranged by size:
5765 94 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3826 4007 4170 3826
Size: 181 163 5532
Fragments arranged by size:
5532 181 163
BtsI GCAGTG_nn'
Cuts at: 831 3119 831
Size: 2288 3588
DraI TTT'AAA
Cuts at: 2875 2875
Size: 5876
DrdI GACnn_nn'nnGTC
Cuts at: 1837 3582 4440 1837
Size: 1745 858 3273
Fragments arranged by size:
3273 1745 858
EaeI y'GGCC_r
Cuts at: 710 889 989 1061 1070 1415 3461 3635 4026
Size: 179 100 72 9 345 2046 174 391
Cuts at: 4026 4053 5398 710
Size: 27 1345 1188
Fragments arranged by size:
2046 1345 1188 391 345 179 174 100
72 27 9
EagI C'GGCC_G
Cuts at: 889 3461 889
Size: 2572 3304
EarI CTCTTCn'nnn_
Cuts at: 1163 2584 3899 4109 1163
Size: 1421 1315 210 2930
Fragments arranged by size:
2930 1421 1315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4084 4394 4540 5686 4084
Size: 310 146 1146 4274
Fragments arranged by size:
4274 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1047 1692 2888 1047
Size: 645 1196 4035
Fragments arranged by size:
4035 1196 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 268 523 607 933 1237 1590 1786 1961
Size: 22 255 84 326 304 353 196 175
Cuts at: 1961 2234 2512 2745 3701 4133 4880 5148 246
Size: 273 278 233 956 432 747 268 974
Fragments arranged by size:
974 956 747 432 353 326 304 278
273 268 255 233 196 175 84 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1542 1542
Size: 5876
EcoO109I rG'GnC_Cy
Cuts at: 396 1023 1705 3015 396
Size: 627 682 1310 3257
Fragments arranged by size:
3257 1310 682 627
EcoRI G'AATT_C
Cuts at: 2880 2880
Size: 5876
EcoRV GAT'ATC
Cuts at: 2757 2757
Size: 5876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3895 3895
Size: 5876
FspI TGC'GCA
Cuts at: 493 673 3657 493
Size: 180 2984 2712
Fragments arranged by size:
2984 2712 180
FspAI rTGC'GCAy
Cuts at: 673 673
Size: 5876
HaeII r_GCGC'y
Cuts at: 163 213 265 384 453 3310 3558 4580 5559
Size: 50 52 119 69 2857 248 1022 979
Cuts at: 5559 163
Size: 480
Fragments arranged by size:
2857 1022 979 480 248 119 69 52
50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 3718 3748 3750 3780 1186
Size: 32 2500 30 2 30 3282
Fragments arranged by size:
3282 2500 32 30 30 2
HincII GTy'rAC
Cuts at: 237 528 2499 2915 237
Size: 291 1971 416 3198
Fragments arranged by size:
3198 1971 416 291
HindIII A'AGCT_T
Cuts at: 1235 1235
Size: 5876
HpaI GTT'AAC
Cuts at: 2499 2499
Size: 5876
KasI G'GCGC_C
Cuts at: 159 261 380 3306 3554 159
Size: 102 119 2926 248 2481
Fragments arranged by size:
2926 2481 248 119 102
KpnI G_GTAC'C
Cuts at: 2896 2896
Size: 5876
MluI A'CGCG_T
Cuts at: 5309 5309
Size: 5876
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4547 4731 4547
Size: 184 5692
MscI TGG'CCA
Cuts at: 991 1063 1072 1417 3637 991
Size: 72 9 345 2220 3230
Fragments arranged by size:
3230 2220 345 72 9
MslI CAynn'nnrTG
Cuts at: 358 484 3865 3992 358
Size: 126 3381 127 2242
Fragments arranged by size:
3381 2242 127 126
MspA1I CmG'CkG
Cuts at: 273 422 564 2987 3301 3661 4674 4919 273
Size: 149 142 2423 314 360 1013 245 1230
Fragments arranged by size:
2423 1230 1013 360 314 245 149 142
NaeI GCC'GGC
Cuts at: 379 4057 379
Size: 3678 2198
NarI GG'CG_CC
Cuts at: 160 262 381 3307 3555 160
Size: 102 119 2926 248 2481
Fragments arranged by size:
2926 2481 248 119 102
NcoI C'CATG_G
Cuts at: 326 326
Size: 5876
NgoMIV G'CCGG_C
Cuts at: 377 4055 377
Size: 3678 2198
NotI GC'GGCC_GC
Cuts at: 889 889
Size: 5876
NspI r_CATG'y
Cuts at: 861 1495 2112 2322 2859 2929 3429 4336 861
Size: 634 617 210 537 70 500 907 2401
Fragments arranged by size:
2401 907 634 617 537 500 210 70
PasI CC'CwG_GG
Cuts at: 1398 1398
Size: 5876
PciI A'CATG_T
Cuts at: 4332 4332
Size: 5876
PmeI GTTT'AAAC
Cuts at: 2875 2875
Size: 5876
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1072 1104 5015 5047 1072
Size: 32 3911 32 1901
Fragments arranged by size:
3911 1901 32 32
PpuMI rG'GwC_Cy
Cuts at: 1023 1705 1023
Size: 682 5194
PsiI TTA'TAA
Cuts at: 2674 2674
Size: 5876
PspOMI G'GGCC_C
Cuts at: 396 396
Size: 5876
PspXI vC'TCGA_Gb
Cuts at: 963 963
Size: 5876
PstI C_TGCA'G
Cuts at: 563 755 2027 2923 3608 563
Size: 192 1272 896 685 2831
Fragments arranged by size:
2831 1272 896 685 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5876
PvuII CAG'CTG
Cuts at: 564 3301 3661 564
Size: 2737 360 2779
Fragments arranged by size:
2779 2737 360
RsrII CG'GwC_CG
Cuts at: 4071 4071
Size: 5876
SacI G_AGCT'C
Cuts at: 1049 1694 2890 1049
Size: 645 1196 4035
Fragments arranged by size:
4035 1196 645
SacII CC_GC'GG
Cuts at: 423 423
Size: 5876
SalI G'TCGA_C
Cuts at: 2913 2913
Size: 5876
SapI GCTCTTCn'nnn_
Cuts at: 3899 4109 3899
Size: 210 5666
SbfI CC_TGCA'GG
Cuts at: 2923 2923
Size: 5876
ScaI AGT'ACT
Cuts at: 5393 5393
Size: 5876
SfcI C'TryA_G
Cuts at: 33 559 751 2023 2165 2919 3604 4597 4788
Size: 526 192 1272 142 754 685 993 191
Cuts at: 4788 33
Size: 1121
Fragments arranged by size:
1272 1121 993 754 685 526 192 191
142
SfiI GGCCn_nnn'nGGCC
Cuts at: 1069 1069
Size: 5876
SfoI GGC'GCC
Cuts at: 161 263 382 3308 3556 161
Size: 102 119 2926 248 2481
Fragments arranged by size:
2926 2481 248 119 102
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5876
SmaI CCC'GGG
Cuts at: 2898 2898
Size: 5876
SmlI C'TyrA_G
Cuts at: 607 963 2595 3031 3946 4438 4700 607
Size: 356 1632 436 915 492 262 1783
Fragments arranged by size:
1783 1632 915 492 436 356 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5876
SphI G_CATG'C
Cuts at: 2859 2929 3429 2859
Size: 70 500 5306
Fragments arranged by size:
5306 500 70
SspI AAT'ATT
Cuts at: 2201 2262 2201
Size: 61 5815
StuI AGG'CCT
Cuts at: 1346 1946 1346
Size: 600 5276
StyI C'CwwG_G
Cuts at: 116 326 1708 2133 3010 3364 116
Size: 210 1382 425 877 354 2628
Fragments arranged by size:
2628 1382 877 425 354 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 1129 1636 3459 3592 4051 4260 610
Size: 519 507 1823 133 459 209 2226
Fragments arranged by size:
2226 1823 519 507 459 209 133
TatI w'GTAC_w
Cuts at: 5391 5391
Size: 5876
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5393 5425 5393
Size: 32 5844
Tth111I GACn'n_nGTC
Cuts at: 3673 3673
Size: 5876
XbaI T'CTAG_A
Cuts at: 2907 2907
Size: 5876
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3371 3371
Size: 5876
XhoI C'TCGA_G
Cuts at: 963 963
Size: 5876
XmaI C'CCGG_G
Cuts at: 2896 2896
Size: 5876
XmnI GAAnn'nnTTC
Cuts at: 1087 1181 1087
Size: 94 5782
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PmeI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsrGI BstZ17I ClaI CspCI DraIII FseI MfeI
NdeI NheI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsrI SanDI SexAI SgrAI SnaBI SrfI SwaI
ZraI