Restriction map for
pF1KE2110
(Circular) (Six-base) MAPSORT of: pF1KE2110.seq Check: 5173 from: 1 to: 5831
pF1KE2110 5831 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 2869 2869
Size: 5831
Acc65I G'GTAC_C
Cuts at: 2847 2847
Size: 5831
AclI AA'CG_TT
Cuts at: 5671 5671
Size: 5831
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 1086 2570 3656 4088 4835 5103 564
Size: 522 1484 1086 432 747 268 1292
Fragments arranged by size:
1484 1292 1086 747 522 432 268
AflIII A'CryG_T
Cuts at: 3183 4287 5264 3183
Size: 1104 977 3750
Fragments arranged by size:
3750 1104 977
AgeI A'CCGG_T
Cuts at: 4219 4219
Size: 5831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2286 2286
Size: 5831
AlwNI CAG_nnn'CTG
Cuts at: 664 1909 3136 4703 664
Size: 1245 1227 1567 1792
Fragments arranged by size:
1792 1567 1245 1227
ApaLI G'TGCA_C
Cuts at: 1308 4601 1308
Size: 3293 2538
ApoI r'AATT_y
Cuts at: 468 537 670 826 955 2367 2788 2835 5184
Size: 69 133 156 129 1412 421 47 2349
Cuts at: 5184 5440 468
Size: 256 859
Fragments arranged by size:
2349 1412 859 421 256 156 133 129
69 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5831
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
AvaI C'yCGr_G
Cuts at: 165 2398 2566 2851 165
Size: 2233 168 285 3145
Fragments arranged by size:
3145 2233 285 168
AvrII C'CTAG_G
Cuts at: 547 2011 547
Size: 1464 4367
BamHI G'GATC_C
Cuts at: 2856 2856
Size: 5831
BanI G'GyrC_C
Cuts at: 1531 1593 2328 2847 3261 3509 3544 1531
Size: 62 735 519 414 248 35 3818
Fragments arranged by size:
3818 735 519 414 248 62 35
BanII G_rGCy'C
Cuts at: 133 166 191 1234 1292 1458 1997 2845 3875
Size: 33 25 1043 58 166 539 848 1030
Cuts at: 3875 133
Size: 2089
Fragments arranged by size:
2089 1043 1030 848 539 166 58 33
25
BbeI G_GCGC'C
Cuts at: 2332 3265 3513 2332
Size: 933 248 4650
Fragments arranged by size:
4650 933 248
BbvCI CC'TCA_GC
Cuts at: 235 2672 235
Size: 2437 3394
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2235 3729 4490 5831 2031
Size: 204 1494 761 1341 2031
Fragments arranged by size:
2031 1494 1341 761 204
BclI T'GATC_A
Cuts at: 3351 3351
Size: 5831
BglI GCCn_nnn'nGGC
Cuts at: 2232 2461 2232
Size: 229 5602
BglII A'GATC_T
Cuts at: 1728 3346 5383 1728
Size: 1618 2037 2176
Fragments arranged by size:
2176 2037 1618
BlpI GC'TnA_GC
Cuts at: 991 2943 991
Size: 1952 3879
Bme1580I G_kGCm'C
Cuts at: 1312 3456 3549 4605 1312
Size: 2144 93 1056 2538
Fragments arranged by size:
2538 2144 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3043 3124 3459 5603 3043
Size: 81 335 2144 3271
Fragments arranged by size:
3271 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1016 1248 4198 1016
Size: 232 2950 2649
Fragments arranged by size:
2950 2649 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 934 1036 1733 1812 2664 784
Size: 150 102 697 79 852 3951
Fragments arranged by size:
3951 852 697 150 102 79
Bpu10I CC'TnA_GC
Cuts at: 235 871 2672 235
Size: 636 1801 3394
Fragments arranged by size:
3394 1801 636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1623 3007 3886 4378 4676 1623
Size: 1384 879 492 298 2778
Fragments arranged by size:
2778 1384 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5535 5535
Size: 5831
BsaAI yAC'GTr
Cuts at: 2297 3184 2297
Size: 887 4944
BsaBI GATnn'nnATC
Cuts at: 6 2528 3345 5382 6
Size: 2522 817 2037 455
Fragments arranged by size:
2522 2037 817 455
BsaHI Gr'CG_yC
Cuts at: 200 1794 2329 3262 3510 4212 5708 200
Size: 1594 535 933 248 702 1496 323
Fragments arranged by size:
1594 1496 933 702 535 323 248
BsaWI w'CCGG_w
Cuts at: 3020 3541 4219 4493 4640 5051 3020
Size: 521 678 274 147 411 3800
Fragments arranged by size:
3800 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1810 1840 1810
Size: 30 5801
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 2416 2490 547
Size: 1869 74 3888
Fragments arranged by size:
3888 1869 74
BseYI C'CCAG_C
Cuts at: 3256 4014 4591 5087 3256
Size: 758 577 496 4000
Fragments arranged by size:
4000 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1549 2456 3373 3419 4627 104
Size: 1445 907 917 46 1208 1308
Fragments arranged by size:
1445 1308 1208 917 907 46
BsiHKAI G_wGCw'C
Cuts at: 133 1312 1458 2845 3623 3813 4605 133
Size: 1179 146 1387 778 190 792 1359
Fragments arranged by size:
1387 1359 1179 792 778 190 146
Bsp1286I G_dGCh'C
Cuts at: 133 166 191 1234 1292 1312 1458 1997 2845
Size: 33 25 1043 58 20 146 539 848
Cuts at: 2845 3456 3549 3623 3813 3875 4605 133
Size: 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 1043 848 730 611 539 190 146
93 74 62 58 33 25 20
BspEI T'CCGG_A
Cuts at: 5051 5051
Size: 5831
BspHI T'CATG_A
Cuts at: 191 1511 2699 5007 191
Size: 1320 1188 2308 1015
Fragments arranged by size:
2308 1320 1188 1015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 808 2881 3095 3397 3778 374
Size: 434 2073 214 302 381 2427
Fragments arranged by size:
2427 2073 434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4123 4177 5661 5826 50
Size: 4073 54 1484 165 55
Fragments arranged by size:
4073 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 810 1702 1975 2185 2312 2744 3743 810
Size: 892 273 210 127 432 999 2898
Fragments arranged by size:
2898 999 892 432 273 210 127
BsrFI r'CCGG_y
Cuts at: 112 1754 1955 2451 3829 4010 4219 112
Size: 1642 201 496 1378 181 209 1724
Fragments arranged by size:
1724 1642 1378 496 209 201 181
BsrGI T'GTAC_A
Cuts at: 1807 1807
Size: 5831
BssSI C'ACGA_G
Cuts at: 2258 4102 4460 2258
Size: 1844 358 3629
Fragments arranged by size:
3629 1844 358
BstAPI GCAn_nnn'nTGC
Cuts at: 664 2383 3208 664
Size: 1719 825 3287
Fragments arranged by size:
3287 1719 825
BstBI TT'CG_AA
Cuts at: 4192 4192
Size: 5831
BstYI r'GATC_y
Cuts at: 1 1401 1728 2856 3323 3346 3681 3927 4939
Size: 1400 327 1128 467 23 335 246 1012
Cuts at: 4939 5025 5037 5383 1
Size: 86 12 346 449
Fragments arranged by size:
1400 1128 1012 467 449 346 335 327
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 770 1565 2590 107
Size: 663 795 1025 3348
Fragments arranged by size:
3348 1025 795 663
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3781 3962 4125 3781
Size: 181 163 5487
Fragments arranged by size:
5487 181 163
BtsI GCAGTG_nn'
Cuts at: 3074 3074
Size: 5831
DraI TTT'AAA
Cuts at: 2830 2830
Size: 5831
DrdI GACnn_nn'nnGTC
Cuts at: 3537 4395 3537
Size: 858 4973
EaeI y'GGCC_r
Cuts at: 110 1546 1756 1885 2372 2453 2768 3416 3590
Size: 1436 210 129 487 81 315 648 174
Cuts at: 3590 3981 4008 5353 110
Size: 391 27 1345 588
Fragments arranged by size:
1436 1345 648 588 487 391 315 210
174 129 81 27
EagI C'GGCC_G
Cuts at: 1546 2453 3416 1546
Size: 907 963 3961
Fragments arranged by size:
3961 963 907
EarI CTCTTCn'nnn_
Cuts at: 121 508 709 1084 1513 1935 2153 2186 2223
Size: 387 201 375 429 422 218 33 37
Cuts at: 2223 3854 4064 121
Size: 1631 210 1888
Fragments arranged by size:
1888 1631 429 422 387 375 218 210
201 37 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4039 4349 4495 5641 4039
Size: 310 146 1146 4229
Fragments arranged by size:
4229 1146 310 146
EcoICRI GAG'CTC
Cuts at: 131 1456 2843 131
Size: 1325 1387 3119
Fragments arranged by size:
3119 1387 1325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 784 934 1036 1086 1733 1812 2570 2664
Size: 220 150 102 50 647 79 758 94
Cuts at: 2664 3656 4088 4835 5103 564
Size: 992 432 747 268 1292
Fragments arranged by size:
1292 992 758 747 647 432 268 220
150 102 94 79 50
EcoNI CCTnn'n_nnAGG
Cuts at: 505 2220 505
Size: 1715 4116
EcoO109I rG'GnC_Cy
Cuts at: 2970 2970
Size: 5831
EcoRI G'AATT_C
Cuts at: 537 2835 537
Size: 2298 3533
EcoRV GAT'ATC
Cuts at: 2484 2484
Size: 5831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 1600 3850 219
Size: 1381 2250 2200
Fragments arranged by size:
2250 2200 1381
FspI TGC'GCA
Cuts at: 3612 3612
Size: 5831
HaeII r_GCGC'y
Cuts at: 337 2332 3265 3513 4535 5514 337
Size: 1995 933 248 1022 979 654
Fragments arranged by size:
1995 1022 979 933 654 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1047 1079 1810 1842 3673 3703 3705 3735 1047
Size: 32 731 32 1831 30 2 30 3143
Fragments arranged by size:
3143 1831 731 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2870 2870
Size: 5831
HindIII A'AGCT_T
Cuts at: 683 1577 2735 683
Size: 894 1158 3779
Fragments arranged by size:
3779 1158 894
KasI G'GCGC_C
Cuts at: 2328 3261 3509 2328
Size: 933 248 4650
Fragments arranged by size:
4650 933 248
KpnI G_GTAC'C
Cuts at: 2851 2851
Size: 5831
MluI A'CGCG_T
Cuts at: 5264 5264
Size: 5831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1266 1749 4502 4686 1266
Size: 483 2753 184 2411
Fragments arranged by size:
2753 2411 483 184
MscI TGG'CCA
Cuts at: 2770 3592 2770
Size: 822 5009
MslI CAynn'nnrTG
Cuts at: 2050 2262 2764 3820 3947 2050
Size: 212 502 1056 127 3934
Fragments arranged by size:
3934 1056 502 212 127
MspA1I CmG'CkG
Cuts at: 152 2592 2624 2942 3256 3616 4629 4874 152
Size: 2440 32 318 314 360 1013 245 1109
Fragments arranged by size:
2440 1109 1013 360 318 314 245 32
NaeI GCC'GGC
Cuts at: 114 2453 4012 114
Size: 2339 1559 1933
Fragments arranged by size:
2339 1933 1559
NarI GG'CG_CC
Cuts at: 2329 3262 3510 2329
Size: 933 248 4650
Fragments arranged by size:
4650 933 248
NcoI C'CATG_G
Cuts at: 107 770 1565 107
Size: 663 795 4373
Fragments arranged by size:
4373 795 663
NgoMIV G'CCGG_C
Cuts at: 112 2451 4010 112
Size: 2339 1559 1933
Fragments arranged by size:
2339 1933 1559
NspI r_CATG'y
Cuts at: 591 2267 2884 3384 4291 591
Size: 1676 617 500 907 2131
Fragments arranged by size:
2131 1676 907 617 500
PciI A'CATG_T
Cuts at: 4287 4287
Size: 5831
PflMI CCAn_nnn'nTGG
Cuts at: 591 591
Size: 5831
PfoI T'CCnGG_A
Cuts at: 2783 2783
Size: 5831
PmeI GTTT'AAAC
Cuts at: 2830 2830
Size: 5831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1616 1648 4970 5002 1616
Size: 32 3322 32 2445
Fragments arranged by size:
3322 2445 32 32
PshAI GACnn'nnGTC
Cuts at: 703 703
Size: 5831
PstI C_TGCA'G
Cuts at: 2878 3563 2878
Size: 685 5146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5831
PvuII CAG'CTG
Cuts at: 2624 3256 3616 2624
Size: 632 360 4839
Fragments arranged by size:
4839 632 360
RsrII CG'GwC_CG
Cuts at: 4026 4026
Size: 5831
SacI G_AGCT'C
Cuts at: 133 1458 2845 133
Size: 1325 1387 3119
Fragments arranged by size:
3119 1387 1325
SacII CC_GC'GG
Cuts at: 2593 2593
Size: 5831
SalI G'TCGA_C
Cuts at: 2868 2868
Size: 5831
SapI GCTCTTCn'nnn_
Cuts at: 121 709 3854 4064 121
Size: 588 3145 210 1888
Fragments arranged by size:
3145 1888 588 210
SbfI CC_TGCA'GG
Cuts at: 2878 2878
Size: 5831
ScaI AGT'ACT
Cuts at: 5348 5348
Size: 5831
SfcI C'TryA_G
Cuts at: 33 2874 3559 4552 4743 33
Size: 2841 685 993 191 1121
Fragments arranged by size:
2841 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2232 2461 2232
Size: 229 5602
SfoI GGC'GCC
Cuts at: 2330 3263 3511 2330
Size: 933 248 4650
Fragments arranged by size:
4650 933 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
SmaI CCC'GGG
Cuts at: 2853 2853
Size: 5831
SmlI C'TyrA_G
Cuts at: 1638 2986 3901 4393 4655 1638
Size: 1348 915 492 262 2814
Fragments arranged by size:
2814 1348 915 492 262
SnaBI TAC'GTA
Cuts at: 2297 2297
Size: 5831
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5831
SphI G_CATG'C
Cuts at: 2884 3384 2884
Size: 500 5331
SspI AAT'ATT
Cuts at: 2316 2316
Size: 5831
StuI AGG'CCT
Cuts at: 2226 2574 2226
Size: 348 5483
StyI C'CwwG_G
Cuts at: 107 547 709 770 1535 1565 1863 2011 2965
Size: 440 162 61 765 30 298 148 954
Cuts at: 2965 3319 107
Size: 354 2619
Fragments arranged by size:
2619 954 765 440 354 298 162 148
61 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3414 3547 4006 4215 3414
Size: 133 459 209 5030
Fragments arranged by size:
5030 459 209 133
TatI w'GTAC_w
Cuts at: 997 1807 2204 5346 997
Size: 810 397 3142 1482
Fragments arranged by size:
3142 1482 810 397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5348 5380 5348
Size: 32 5799
Tth111I GACn'n_nGTC
Cuts at: 3628 3628
Size: 5831
XbaI T'CTAG_A
Cuts at: 2862 2862
Size: 5831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2130 2389 3326 2130
Size: 259 937 4635
Fragments arranged by size:
4635 937 259
XmaI C'CCGG_G
Cuts at: 2851 2851
Size: 5831
XmnI GAAnn'nnTTC
Cuts at: 1662 1662
Size: 5831
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SrfI
SwaI XhoI ZraI