Restriction map for
pF1KE2076
(Circular) (Six-base) MAPSORT of: pF1KE2076.seq Check: 4286 from: 1 to: 5348
pF1KE2076 5348 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 1764 1764
Size: 5348
AccI GT'mk_AC
Cuts at: 2386 2386
Size: 5348
Acc65I G'GTAC_C
Cuts at: 2364 2364
Size: 5348
AclI AA'CG_TT
Cuts at: 5188 5188
Size: 5348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 547 1412 1601 1817 2150 2207 3173 3605
Size: 179 865 189 216 333 57 966 432
Cuts at: 3605 4352 4620 368
Size: 747 268 1096
Fragments arranged by size:
1096 966 865 747 432 333 268 216
189 179 57
AflIII A'CryG_T
Cuts at: 2700 3804 4781 2700
Size: 1104 977 3267
Fragments arranged by size:
3267 1104 977
AgeI A'CCGG_T
Cuts at: 3736 3736
Size: 5348
AlwNI CAG_nnn'CTG
Cuts at: 669 709 2653 4220 669
Size: 40 1944 1567 1797
Fragments arranged by size:
1944 1797 1567 40
ApaI G_GGCC'C
Cuts at: 149 149
Size: 5348
ApaLI G'TGCA_C
Cuts at: 1399 1879 4118 1399
Size: 480 2239 2629
Fragments arranged by size:
2629 2239 480
ApoI r'AATT_y
Cuts at: 1032 1184 2244 2352 4701 4957 1032
Size: 152 1060 108 2349 256 1423
Fragments arranged by size:
2349 1423 1060 256 152 108
AseI AT'TA_AT
Cuts at: 20 1015 20
Size: 995 4353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
AvaI C'yCGr_G
Cuts at: 120 2368 120
Size: 2248 3100
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 649 682 649
Size: 33 5315
BamHI G'GATC_C
Cuts at: 819 1194 2110 2373 819
Size: 375 916 263 3794
Fragments arranged by size:
3794 916 375 263
BanI G'GyrC_C
Cuts at: 172 281 2364 2778 3026 3061 172
Size: 109 2083 414 248 35 2459
Fragments arranged by size:
2459 2083 414 248 109 35
BanII G_rGCy'C
Cuts at: 149 683 1750 2362 3392 149
Size: 534 1067 612 1030 2105
Fragments arranged by size:
2105 1067 1030 612 534
BbeI G_GCGC'C
Cuts at: 2782 3030 2782
Size: 248 5100
BbsI GAAGACnn'nnnn_
Cuts at: 1099 1430 1462 1764 1099
Size: 331 32 302 4683
Fragments arranged by size:
4683 331 302 32
BbvCI CC'TCA_GC
Cuts at: 959 1673 959
Size: 714 4634
BciVI GTATCCnnnnn_n'
Cuts at: 1684 3246 4007 5348 1684
Size: 1562 761 1341 1684
Fragments arranged by size:
1684 1562 1341 761
BclI T'GATC_A
Cuts at: 624 2178 2868 624
Size: 1554 690 3104
Fragments arranged by size:
3104 1554 690
BglI GCCn_nnn'nGGC
Cuts at: 123 123
Size: 5348
BglII A'GATC_T
Cuts at: 411 537 2863 4900 411
Size: 126 2326 2037 859
Fragments arranged by size:
2326 2037 859 126
BlpI GC'TnA_GC
Cuts at: 2460 2460
Size: 5348
Bme1580I G_kGCm'C
Cuts at: 149 175 1403 1883 2973 3066 4122 149
Size: 26 1228 480 1090 93 1056 1375
Fragments arranged by size:
1375 1228 1090 1056 480 93 26
BmrI ACTGGGnnnn_n'
Cuts at: 2560 2641 2976 5120 2560
Size: 81 335 2144 2788
Fragments arranged by size:
2788 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 430 3715 430
Size: 3285 2063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1717 425
Size: 1292 4056
Bpu10I CC'TnA_GC
Cuts at: 959 1673 959
Size: 714 4634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1894 2192 2524 3403 3895 4193 1145
Size: 749 298 332 879 492 298 2300
Fragments arranged by size:
2300 879 749 492 332 298 298
BsaI GGTCTCn'nnnn_
Cuts at: 5052 5052
Size: 5348
BsaAI yAC'GTr
Cuts at: 2701 2701
Size: 5348
BsaBI GATnn'nnATC
Cuts at: 6 623 2862 4899 6
Size: 617 2239 2037 455
Fragments arranged by size:
2239 2037 617 455
BsaHI Gr'CG_yC
Cuts at: 1761 2779 3027 3729 5225 1761
Size: 1018 248 702 1496 1884
Fragments arranged by size:
1884 1496 1018 702 248
BsaWI w'CCGG_w
Cuts at: 2537 3058 3736 4010 4157 4568 2537
Size: 521 678 274 147 411 3317
Fragments arranged by size:
3317 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 962 992 962
Size: 30 5318
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 422 983 2195 422
Size: 561 1212 3575
Fragments arranged by size:
3575 1212 561
BseYI C'CCAG_C
Cuts at: 168 2773 3531 4108 4604 168
Size: 2605 758 577 496 912
Fragments arranged by size:
2605 912 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2147 1909
Size: 238 5110
BsiEI CG_ry'CG
Cuts at: 104 133 2890 2936 4144 104
Size: 29 2757 46 1208 1308
Fragments arranged by size:
2757 1308 1208 46 29
BsiHKAI G_wGCw'C
Cuts at: 683 1403 1883 1910 2362 3140 3330 4122 683
Size: 720 480 27 452 778 190 792 1909
Fragments arranged by size:
1909 792 778 720 480 452 190 27
BsmI GAATG_Cn'
Cuts at: 1565 1565
Size: 5348
Bsp1286I G_dGCh'C
Cuts at: 149 175 683 1403 1750 1883 1910 2362 2973
Size: 26 508 720 347 133 27 452 611
Cuts at: 2973 3066 3140 3330 3392 4122 149
Size: 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 720 611 508 452 347 190
133 93 74 62 27 26
BspEI T'CCGG_A
Cuts at: 4568 4568
Size: 5348
BspHI T'CATG_A
Cuts at: 369 2280 4524 369
Size: 1911 2244 1193
Fragments arranged by size:
2244 1911 1193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2398 2612 2914 3295 2398
Size: 214 302 381 4451
Fragments arranged by size:
4451 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 165 3640 3694 5178 5343 50
Size: 64 51 3475 54 1484 165 55
Fragments arranged by size:
3475 1484 165 64 55 54 51
BsrDI GCAATG_nn'
Cuts at: 395 1466 3260 395
Size: 1071 1794 2483
Fragments arranged by size:
2483 1794 1071
BsrFI r'CCGG_y
Cuts at: 175 3346 3527 3736 175
Size: 3171 181 209 1787
Fragments arranged by size:
3171 1787 209 181
BsrGI T'GTAC_A
Cuts at: 640 1338 2080 640
Size: 698 742 3908
Fragments arranged by size:
3908 742 698
BssSI C'ACGA_G
Cuts at: 3619 3977 3619
Size: 358 4990
BstAPI GCAn_nnn'nTGC
Cuts at: 780 1887 2725 780
Size: 1107 838 3403
Fragments arranged by size:
3403 1107 838
BstBI TT'CG_AA
Cuts at: 3709 3709
Size: 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 794 794
Size: 5348
BstYI r'GATC_y
Cuts at: 1 411 537 819 1194 2110 2373 2840 2863
Size: 410 126 282 375 916 263 467 23
Cuts at: 2863 3198 3444 4456 4542 4554 4900 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 916 467 449 410 375 346 335
282 263 246 126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1976 1976
Size: 5348
BtgI C'CryG_G
Cuts at: 107 734 920 107
Size: 627 186 4535
Fragments arranged by size:
4535 627 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3298 3479 3642 3298
Size: 181 163 5004
Fragments arranged by size:
5004 181 163
BtsI GCAGTG_nn'
Cuts at: 658 1403 2591 658
Size: 745 1188 3415
Fragments arranged by size:
3415 1188 745
DraI TTT'AAA
Cuts at: 2347 2347
Size: 5348
DraIII CAC_nnn'GTG
Cuts at: 1407 1887 1407
Size: 480 4868
DrdI GACnn_nn'nnGTC
Cuts at: 2020 3054 3912 2020
Size: 1034 858 3456
Fragments arranged by size:
3456 1034 858
EaeI y'GGCC_r
Cuts at: 130 2259 2933 3107 3498 3525 4870 130
Size: 2129 674 174 391 27 1345 608
Fragments arranged by size:
2129 1345 674 608 391 174 27
EagI C'GGCC_G
Cuts at: 130 2933 130
Size: 2803 2545
EarI CTCTTCn'nnn_
Cuts at: 386 839 1738 3371 3581 386
Size: 453 899 1633 210 2153
Fragments arranged by size:
2153 1633 899 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3556 3866 4012 5158 3556
Size: 310 146 1146 3746
Fragments arranged by size:
3746 1146 310 146
EcoICRI GAG'CTC
Cuts at: 681 2360 681
Size: 1679 3669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 425 547 1412 1601 1717 1817 2150 2207
Size: 57 122 865 189 116 100 333 57
Cuts at: 2207 3173 3605 4352 4620 368
Size: 966 432 747 268 1096
Fragments arranged by size:
1096 966 865 747 432 333 268 189
122 116 100 57 57
EcoO109I rG'GnC_Cy
Cuts at: 146 954 973 2487 146
Size: 808 19 1514 3007
Fragments arranged by size:
3007 1514 808 19
EcoRI G'AATT_C
Cuts at: 2352 2352
Size: 5348
EcoRV GAT'ATC
Cuts at: 1613 1613
Size: 5348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1186 3367 1186
Size: 2181 3167
FspI TGC'GCA
Cuts at: 3129 3129
Size: 5348
HaeII r_GCGC'y
Cuts at: 2782 3030 4052 5031 2782
Size: 248 1022 979 3099
Fragments arranged by size:
3099 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 1767 1799 1950 1982 3190 3220 3222
Size: 32 1257 32 151 32 1208 30 2
Cuts at: 3222 3252 478
Size: 30 2574
Fragments arranged by size:
2574 1257 1208 151 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 981 2336 2387 981
Size: 1355 51 3942
Fragments arranged by size:
3942 1355 51
HpaI GTT'AAC
Cuts at: 2336 2336
Size: 5348
KasI G'GCGC_C
Cuts at: 2778 3026 2778
Size: 248 5100
KpnI G_GTAC'C
Cuts at: 2368 2368
Size: 5348
MluI A'CGCG_T
Cuts at: 4781 4781
Size: 5348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4019 4203 4019
Size: 184 5164
MscI TGG'CCA
Cuts at: 2261 3109 2261
Size: 848 4500
MslI CAynn'nnrTG
Cuts at: 320 1861 2029 2148 3337 3464 320
Size: 1541 168 119 1189 127 2204
Fragments arranged by size:
2204 1541 1189 168 127 119
MspA1I CmG'CkG
Cuts at: 1374 2459 2773 3133 4146 4391 1374
Size: 1085 314 360 1013 245 2331
Fragments arranged by size:
2331 1085 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3529 3529
Size: 5348
NarI GG'CG_CC
Cuts at: 2779 3027 2779
Size: 248 5100
NcoI C'CATG_G
Cuts at: 107 734 920 107
Size: 627 186 4535
Fragments arranged by size:
4535 627 186
NgoMIV G'CCGG_C
Cuts at: 3527 3527
Size: 5348
NspI r_CATG'y
Cuts at: 842 1723 2401 2901 3808 842
Size: 881 678 500 907 2382
Fragments arranged by size:
2382 907 881 678 500
PasI CC'CwG_GG
Cuts at: 149 149
Size: 5348
PciI A'CATG_T
Cuts at: 3804 3804
Size: 5348
PmeI GTTT'AAAC
Cuts at: 2347 2347
Size: 5348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 206 238 4487 4519 206
Size: 32 4249 32 1035
Fragments arranged by size:
4249 1035 32 32
PpuMI rG'GwC_Cy
Cuts at: 954 973 954
Size: 19 5329
PspOMI G'GGCC_C
Cuts at: 145 145
Size: 5348
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2073 2105 2073
Size: 32 5316
PstI C_TGCA'G
Cuts at: 665 785 2395 3080 665
Size: 120 1610 685 2933
Fragments arranged by size:
2933 1610 685 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5348
PvuII CAG'CTG
Cuts at: 1374 2773 3133 1374
Size: 1399 360 3589
Fragments arranged by size:
3589 1399 360
RsrII CG'GwC_CG
Cuts at: 3543 3543
Size: 5348
SacI G_AGCT'C
Cuts at: 683 2362 683
Size: 1679 3669
SalI G'TCGA_C
Cuts at: 2385 2385
Size: 5348
SanDI GG'GwC_CC
Cuts at: 954 954
Size: 5348
SapI GCTCTTCn'nnn_
Cuts at: 1738 3371 3581 1738
Size: 1633 210 3505
Fragments arranged by size:
3505 1633 210
SbfI CC_TGCA'GG
Cuts at: 2395 2395
Size: 5348
ScaI AGT'ACT
Cuts at: 4865 4865
Size: 5348
SfcI C'TryA_G
Cuts at: 33 661 781 934 1117 1428 1738 1845 2391
Size: 628 120 153 183 311 310 107 546
Cuts at: 2391 3076 4069 4260 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 546 311 310 191
183 153 120 107
SfoI GGC'GCC
Cuts at: 2780 3028 2780
Size: 248 5100
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
SgrAI Cr'CCGG_yG
Cuts at: 175 175
Size: 5348
SmaI CCC'GGG
Cuts at: 122 2370 122
Size: 2248 3100
SmlI C'TyrA_G
Cuts at: 1160 1909 2207 2503 3418 3910 4172 1160
Size: 749 298 296 915 492 262 2336
Fragments arranged by size:
2336 915 749 492 298 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5348
SphI G_CATG'C
Cuts at: 1723 2401 2901 1723
Size: 678 500 4170
Fragments arranged by size:
4170 678 500
SspI AAT'ATT
Cuts at: 360 360
Size: 5348
StuI AGG'CCT
Cuts at: 334 765 334
Size: 431 4917
StyI C'CwwG_G
Cuts at: 107 734 920 2256 2482 2836 107
Size: 627 186 1336 226 354 2619
Fragments arranged by size:
2619 1336 627 354 226 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2931 3064 3523 3732 2931
Size: 133 459 209 4547
Fragments arranged by size:
4547 459 209 133
TatI w'GTAC_w
Cuts at: 640 1044 1338 2080 4863 640
Size: 404 294 742 2783 1125
Fragments arranged by size:
2783 1125 742 404 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 811 843 4865 4897 811
Size: 32 4022 32 1262
Fragments arranged by size:
4022 1262 32 32
Tth111I GACn'n_nGTC
Cuts at: 3145 3145
Size: 5348
XbaI T'CTAG_A
Cuts at: 1224 2118 2379 1224
Size: 894 261 4193
Fragments arranged by size:
4193 894 261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2843 2843
Size: 5348
XmaI C'CCGG_G
Cuts at: 120 2368 120
Size: 2248 3100
XmnI GAAnn'nnTTC
Cuts at: 1106 1106
Size: 5348
ZraI GAC'GTC
Cuts at: 1762 1762
Size: 5348
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I ClaI CspCI EcoNI FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI SacII SexAI SfiI
SnaBI SrfI SwaI XhoI