Restriction map for
pF1KE1989
(Circular) (Six-base) MAPSORT of: pF1KE1989.seq Check: 9806 from: 1 to: 4808
pF1KE1989 4808 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 911 1667 1846 911
Size: 756 179 3873
Fragments arranged by size:
3873 756 179
Acc65I G'GTAC_C
Cuts at: 1824 1824
Size: 4808
AclI AA'CG_TT
Cuts at: 4648 4648
Size: 4808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1101 2633 3065 3812 4080 1101
Size: 1532 432 747 268 1829
Fragments arranged by size:
1829 1532 747 432 268
AfeI AGC'GCT
Cuts at: 922 1033 1582 922
Size: 111 549 4148
Fragments arranged by size:
4148 549 111
AflIII A'CryG_T
Cuts at: 829 2160 3264 4241 829
Size: 1331 1104 977 1396
Fragments arranged by size:
1396 1331 1104 977
AgeI A'CCGG_T
Cuts at: 3196 3196
Size: 4808
AleI CACnn'nnGTG
Cuts at: 402 973 1060 402
Size: 571 87 4150
Fragments arranged by size:
4150 571 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 142 420 647 1078 1756 142
Size: 278 227 431 678 3194
Fragments arranged by size:
3194 678 431 278 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 410 442 410
Size: 32 4776
AlwNI CAG_nnn'CTG
Cuts at: 2113 3680 2113
Size: 1567 3241
ApaLI G'TGCA_C
Cuts at: 246 325 405 3578 246
Size: 79 80 3173 1476
Fragments arranged by size:
3173 1476 80 79
ApoI r'AATT_y
Cuts at: 1812 4161 4417 1812
Size: 2349 256 2203
Fragments arranged by size:
2349 2203 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4808
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
AvaI C'yCGr_G
Cuts at: 505 645 654 1828 505
Size: 140 9 1174 3485
Fragments arranged by size:
3485 1174 140 9
BamHI G'GATC_C
Cuts at: 848 1833 848
Size: 985 3823
BanI G'GyrC_C
Cuts at: 541 631 640 726 1069 1453 1824 2238 2486
Size: 90 9 86 343 384 371 414 248
Cuts at: 2486 2521 541
Size: 35 2828
Fragments arranged by size:
2828 414 384 371 343 248 90 86
35 9
BanII G_rGCy'C
Cuts at: 588 1822 2852 588
Size: 1234 1030 2544
Fragments arranged by size:
2544 1234 1030
BbeI G_GCGC'C
Cuts at: 545 2242 2490 545
Size: 1697 248 2863
Fragments arranged by size:
2863 1697 248
BbsI GAAGACnn'nnnn_
Cuts at: 1491 1491
Size: 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 237 271 90
Size: 34 113 34 4627
Fragments arranged by size:
4627 113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2706 3467 4808 2706
Size: 761 1341 2706
Fragments arranged by size:
2706 1341 761
BclI T'GATC_A
Cuts at: 1673 2328 1673
Size: 655 4153
BglI GCCn_nnn'nGGC
Cuts at: 172 659 1204 1237 1246 172
Size: 487 545 33 9 3734
Fragments arranged by size:
3734 545 487 33 9
BglII A'GATC_T
Cuts at: 551 2323 4360 551
Size: 1772 2037 999
Fragments arranged by size:
2037 1772 999
BlpI GC'TnA_GC
Cuts at: 1300 1920 1300
Size: 620 4188
Bme1580I G_kGCm'C
Cuts at: 250 329 409 643 1072 1523 1627 2433 2526
Size: 79 80 234 429 451 104 806 93
Cuts at: 2526 3582 250
Size: 1056 1476
Fragments arranged by size:
1476 1056 806 451 429 234 104 93
80 79
BmgBI CAC'GTC
Cuts at: 1391 1709 1391
Size: 318 4490
BmrI ACTGGGnnnn_n'
Cuts at: 171 1584 2020 2101 2436 4580 171
Size: 1413 436 81 335 2144 399
Fragments arranged by size:
2144 1413 436 399 335 81
BmtI G_CTAG'C
Cuts at: 1360 1360
Size: 4808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1053 1227 3175 1053
Size: 174 1948 2686
Fragments arranged by size:
2686 1948 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 991 1492 712
Size: 279 501 4028
Fragments arranged by size:
4028 501 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1984 2863 3355 3653 706
Size: 1278 879 492 298 1861
Fragments arranged by size:
1861 1278 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1786 4512 1786
Size: 2726 2082
BsaAI yAC'GTr
Cuts at: 902 2161 902
Size: 1259 3549
BsaBI GATnn'nnATC
Cuts at: 6 2322 4359 6
Size: 2316 2037 455
Fragments arranged by size:
2316 2037 455
BsaHI Gr'CG_yC
Cuts at: 542 1496 2239 2487 3189 4685 542
Size: 954 743 248 702 1496 665
Fragments arranged by size:
1496 954 743 702 665 248
BsaWI w'CCGG_w
Cuts at: 1997 2518 3196 3470 3617 4028 1997
Size: 521 678 274 147 411 2777
Fragments arranged by size:
2777 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1570 1600 1570
Size: 30 4778
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 838 838
Size: 4808
BseYI C'CCAG_C
Cuts at: 2233 2991 3568 4064 2233
Size: 758 577 496 2977
Fragments arranged by size:
2977 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1071 1084 1726 373
Size: 698 13 642 3455
Fragments arranged by size:
3455 698 642 13
BsiEI CG_ry'CG
Cuts at: 104 1250 2350 2396 3604 104
Size: 1146 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1146 1100 46
BsiHKAI G_wGCw'C
Cuts at: 250 329 361 388 409 527 588 698 1007
Size: 79 32 27 21 118 61 110 309
Cuts at: 1007 1511 1574 1822 2600 2790 3582 250
Size: 504 63 248 778 190 792 1476
Fragments arranged by size:
1476 792 778 504 309 248 190 118
110 79 63 61 32 27 21
BsmBI CGTCTCn'nnnn_
Cuts at: 820 820
Size: 4808
Bsp1286I G_dGCh'C
Cuts at: 250 329 361 388 409 527 588 643 698
Size: 79 32 27 21 118 61 55 55
Cuts at: 698 1007 1072 1511 1523 1574 1627 1822 2433
Size: 309 65 439 12 51 53 195 611
Cuts at: 2433 2526 2600 2790 2852 3582 250
Size: 93 74 190 62 730 1476
Fragments arranged by size:
1476 730 611 439 309 195 190 118
93 79 74 65 62 61 55 55
53 51 32 27 21 12
BspEI T'CCGG_A
Cuts at: 4028 4028
Size: 4808
BspHI T'CATG_A
Cuts at: 479 1100 1439 1685 3984 479
Size: 621 339 246 2299 1303
Fragments arranged by size:
2299 1303 621 339 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 795 1304 1858 2072 2374 2755 795
Size: 509 554 214 302 381 2848
Fragments arranged by size:
2848 554 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 440 939 1304 1365 1476 3100 3154 4638
Size: 390 499 365 61 111 1624 54 1484
Cuts at: 4638 4803 50
Size: 165 55
Fragments arranged by size:
1624 1484 499 390 365 165 111 61
55 54
BsrDI GCAATG_nn'
Cuts at: 2720 2720
Size: 4808
BsrFI r'CCGG_y
Cuts at: 171 1174 1245 1405 2806 2987 3196 171
Size: 1003 71 160 1401 181 209 1783
Fragments arranged by size:
1783 1401 1003 209 181 160 71
BssHII G'CGCG_C
Cuts at: 453 455 457 838 453
Size: 2 2 381 4423
Fragments arranged by size:
4423 381 2 2
BssSI C'ACGA_G
Cuts at: 1192 1584 1746 3079 3437 1192
Size: 392 162 1333 358 2563
Fragments arranged by size:
2563 1333 392 358 162
BstAPI GCAn_nnn'nTGC
Cuts at: 2185 2185
Size: 4808
BstBI TT'CG_AA
Cuts at: 3169 3169
Size: 4808
BstEII G'GTnAC_C
Cuts at: 1048 1048
Size: 4808
BstXI CCAn_nnnn'nTGG
Cuts at: 288 906 288
Size: 618 4190
BstYI r'GATC_y
Cuts at: 1 551 848 1721 1833 2300 2323 2658 2904
Size: 550 297 873 112 467 23 335 246
Cuts at: 2904 3916 4002 4014 4360 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 873 550 467 449 346 335 297
246 112 86 23 12
BtgI C'CryG_G
Cuts at: 183 213 537 971 1274 1799 183
Size: 30 324 434 303 525 3192
Fragments arranged by size:
3192 525 434 324 303 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 240 975 1521 2758 2939 3102 240
Size: 735 546 1237 181 163 1946
Fragments arranged by size:
1946 1237 735 546 181 163
BtsI GCAGTG_nn'
Cuts at: 361 705 1599 2051 361
Size: 344 894 452 3118
Fragments arranged by size:
3118 894 452 344
DraI TTT'AAA
Cuts at: 1807 1807
Size: 4808
DrdI GACnn_nn'nnGTC
Cuts at: 1663 2514 3372 1663
Size: 851 858 3099
Fragments arranged by size:
3099 858 851
EaeI y'GGCC_r
Cuts at: 561 1124 1229 1247 1271 1403 1613 2393 2567
Size: 563 105 18 24 132 210 780 174
Cuts at: 2567 2958 2985 4330 561
Size: 391 27 1345 1039
Fragments arranged by size:
1345 1039 780 563 391 210 174 132
105 27 24 18
EagI C'GGCC_G
Cuts at: 1247 2393 1247
Size: 1146 3662
EarI CTCTTCn'nnn_
Cuts at: 1102 1372 1426 2831 3041 1102
Size: 270 54 1405 210 2869
Fragments arranged by size:
2869 1405 270 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3016 3326 3472 4618 3016
Size: 310 146 1146 3206
Fragments arranged by size:
3206 1146 310 146
EcoICRI GAG'CTC
Cuts at: 586 1820 586
Size: 1234 3574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 991 1101 1492 2633 3065 3812 4080 712
Size: 279 110 391 1141 432 747 268 1440
Fragments arranged by size:
1440 1141 747 432 391 279 268 110
EcoO109I rG'GnC_Cy
Cuts at: 1947 1947
Size: 4808
EcoRI G'AATT_C
Cuts at: 1812 1812
Size: 4808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4808
FseI GG_CCGG'CC
Cuts at: 175 175
Size: 4808
FspI TGC'GCA
Cuts at: 1489 2589 1489
Size: 1100 3708
FspAI rTGC'GCAy
Cuts at: 1489 1489
Size: 4808
HaeII r_GCGC'y
Cuts at: 125 513 545 671 924 1035 1363 1584 2242
Size: 388 32 126 253 111 328 221 658
Cuts at: 2242 2490 3512 4491 125
Size: 248 1022 979 442
Fragments arranged by size:
1022 979 658 442 388 328 253 248
221 126 111 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2650 2680 2682 2712 2650
Size: 30 2 30 4746
Fragments arranged by size:
4746 30 30 2
HincII GTy'rAC
Cuts at: 1847 1847
Size: 4808
KasI G'GCGC_C
Cuts at: 541 2238 2486 541
Size: 1697 248 2863
Fragments arranged by size:
2863 1697 248
KpnI G_GTAC'C
Cuts at: 1828 1828
Size: 4808
MluI A'CGCG_T
Cuts at: 829 4241 829
Size: 3412 1396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3479 3663 3479
Size: 184 4624
MscI TGG'CCA
Cuts at: 1126 1273 1615 2569 1126
Size: 147 342 954 3365
Fragments arranged by size:
3365 954 342 147
MslI CAynn'nnrTG
Cuts at: 402 973 1060 1438 2797 2924 402
Size: 571 87 378 1359 127 2286
Fragments arranged by size:
2286 1359 571 378 127 87
MspA1I CmG'CkG
Cuts at: 147 243 342 1341 1919 2233 2593 3606 3851
Size: 96 99 999 578 314 360 1013 245
Cuts at: 3851 147
Size: 1104
Fragments arranged by size:
1104 1013 999 578 360 314 245 99
96
NaeI GCC'GGC
Cuts at: 173 1176 1247 1407 2989 173
Size: 1003 71 160 1582 1992
Fragments arranged by size:
1992 1582 1003 160 71
NarI GG'CG_CC
Cuts at: 542 2239 2487 542
Size: 1697 248 2863
Fragments arranged by size:
2863 1697 248
NcoI C'CATG_G
Cuts at: 971 1274 971
Size: 303 4505
NgoMIV G'CCGG_C
Cuts at: 171 1174 1245 1405 2987 171
Size: 1003 71 160 1582 1992
Fragments arranged by size:
1992 1582 1003 160 71
NheI G'CTAG_C
Cuts at: 1356 1356
Size: 4808
NspI r_CATG'y
Cuts at: 918 1650 1861 2361 3268 918
Size: 732 211 500 907 2458
Fragments arranged by size:
2458 907 732 500 211
PciI A'CATG_T
Cuts at: 3264 3264
Size: 4808
PflMI CCAn_nnn'nTGG
Cuts at: 1139 1139
Size: 4808
PfoI T'CCnGG_A
Cuts at: 206 1007 206
Size: 801 4007
PmeI GTTT'AAAC
Cuts at: 1807 1807
Size: 4808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 4776
PstI C_TGCA'G
Cuts at: 626 1855 2540 626
Size: 1229 685 2894
Fragments arranged by size:
2894 1229 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4808
PvuII CAG'CTG
Cuts at: 2233 2593 2233
Size: 360 4448
RsrII CG'GwC_CG
Cuts at: 3003 3003
Size: 4808
SacI G_AGCT'C
Cuts at: 588 1822 588
Size: 1234 3574
SalI G'TCGA_C
Cuts at: 1845 1845
Size: 4808
SapI GCTCTTCn'nnn_
Cuts at: 1372 1426 2831 3041 1372
Size: 54 1405 210 3139
Fragments arranged by size:
3139 1405 210 54
SbfI CC_TGCA'GG
Cuts at: 1855 1855
Size: 4808
ScaI AGT'ACT
Cuts at: 1219 4325 1219
Size: 3106 1702
SexAI A'CCwGG_T
Cuts at: 856 1159 856
Size: 303 4505
SfcI C'TryA_G
Cuts at: 33 622 1851 2536 3529 3720 33
Size: 589 1229 685 993 191 1121
Fragments arranged by size:
1229 1121 993 685 589 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1204 1237 1246 1204
Size: 33 9 4766
Fragments arranged by size:
4766 33 9
SfoI GGC'GCC
Cuts at: 543 2240 2488 543
Size: 1697 248 2863
Fragments arranged by size:
2863 1697 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
SmaI CCC'GGG
Cuts at: 647 656 1830 647
Size: 9 1174 3625
Fragments arranged by size:
3625 1174 9
SmlI C'TyrA_G
Cuts at: 721 1963 2878 3370 3632 721
Size: 1242 915 492 262 1897
Fragments arranged by size:
1897 1242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4808
SphI G_CATG'C
Cuts at: 1861 2361 1861
Size: 500 4308
SrfI GCCC'GGGC
Cuts at: 656 656
Size: 4808
StuI AGG'CCT
Cuts at: 317 1322 1561 317
Size: 1005 239 3564
Fragments arranged by size:
3564 1005 239
StyI C'CwwG_G
Cuts at: 971 1274 1942 2296 971
Size: 303 668 354 3483
Fragments arranged by size:
3483 668 354 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1477 1578 2391 2524 2983 3192 1477
Size: 101 813 133 459 209 3093
Fragments arranged by size:
3093 813 459 209 133 101
TatI w'GTAC_w
Cuts at: 1217 4323 1217
Size: 3106 1702
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1570 1602 4325 4357 1570
Size: 32 2723 32 2021
Fragments arranged by size:
2723 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 2605 2605
Size: 4808
XbaI T'CTAG_A
Cuts at: 1839 1839
Size: 4808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 978 1266 1272 2303 978
Size: 288 6 1031 3483
Fragments arranged by size:
3483 1031 288 6
XmaI C'CCGG_G
Cuts at: 645 654 1828 645
Size: 9 1174 3625
Fragments arranged by size:
3625 1174 9
XmnI GAAnn'nnTTC
Cuts at: 487 487
Size: 4808
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BaeI
BbvCI BfrBI Bpu10I BsiWI BsmI BsrGI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SgrAI
SnaBI SspI SwaI XhoI ZraI