Restriction map for
pF1KE1965
(Circular) (Six-base) MAPSORT of: pF1KE1965.seq Check: 3105 from: 1 to: 4700
pF1KE1965 4700 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1034 1652 2525 2957 3704 3972 1034
Size: 618 873 432 747 268 1762
Fragments arranged by size:
1762 873 747 618 432 268
AflIII A'CryG_T
Cuts at: 2052 3156 4133 2052
Size: 1104 977 2619
Fragments arranged by size:
2619 1104 977
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AhdI GACnn_n'nnGTC
Cuts at: 339 339
Size: 4700
AleI CACnn'nnGTG
Cuts at: 325 325
Size: 4700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 755 787 755
Size: 32 4668
AlwNI CAG_nnn'CTG
Cuts at: 780 2005 3572 780
Size: 1225 1567 1908
Fragments arranged by size:
1908 1567 1225
ApaLI G'TGCA_C
Cuts at: 3470 3470
Size: 4700
ApoI r'AATT_y
Cuts at: 505 1063 1704 4053 4309 505
Size: 558 641 2349 256 896
Fragments arranged by size:
2349 896 641 558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 985 1720 985
Size: 735 3965
BamHI G'GATC_C
Cuts at: 1725 1725
Size: 4700
BanI G'GyrC_C
Cuts at: 1316 1716 2130 2378 2413 1316
Size: 400 414 248 35 3603
Fragments arranged by size:
3603 414 400 248 35
BanII G_rGCy'C
Cuts at: 1714 2744 1714
Size: 1030 3670
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 469 503 90
Size: 34 345 34 4287
Fragments arranged by size:
4287 345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BfrBI ATG'CAT
Cuts at: 645 1279 645
Size: 634 4066
BglI GCCn_nnn'nGGC
Cuts at: 1582 1582
Size: 4700
BglII A'GATC_T
Cuts at: 1437 2215 4252 1437
Size: 778 2037 1885
Fragments arranged by size:
2037 1885 778
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 492 2325 2418 3474 492
Size: 1833 93 1056 1718
Fragments arranged by size:
1833 1718 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1172 1912 1993 2328 4472 1172
Size: 740 81 335 2144 1400
Fragments arranged by size:
2144 1400 740 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 711
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1876 2755 3247 3545 1876
Size: 879 492 298 3031
Fragments arranged by size:
3031 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4404 4404
Size: 4700
BsaAI yAC'GTr
Cuts at: 2053 2053
Size: 4700
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 2131 2379 3081 4577 2131
Size: 248 702 1496 2254
Fragments arranged by size:
2254 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1889 2410 3088 3362 3509 3920 1889
Size: 521 678 274 147 411 2669
Fragments arranged by size:
2669 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 228 258 228
Size: 30 4670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 174 906 961 1639 174
Size: 732 55 678 3235
Fragments arranged by size:
3235 732 678 55
BseYI C'CCAG_C
Cuts at: 900 2125 2883 3460 3956 900
Size: 1225 758 577 496 1644
Fragments arranged by size:
1644 1225 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 426
Size: 4700
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 382 1714 2492 2682 3474 382
Size: 1332 778 190 792 1608
Fragments arranged by size:
1608 1332 792 778 190
BsmI GAATG_Cn'
Cuts at: 214 214
Size: 4700
BsmBI CGTCTCn'nnnn_
Cuts at: 444 444
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 382 492 1714 2325 2418 2492 2682 2744 3474
Size: 110 1222 611 93 74 190 62 730
Cuts at: 3474 382
Size: 1608
Fragments arranged by size:
1608 1222 730 611 190 110 93 74
62
BspEI T'CCGG_A
Cuts at: 3920 3920
Size: 4700
BspHI T'CATG_A
Cuts at: 3876 3876
Size: 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 379 1750 1964 2266 2647 379
Size: 1371 214 302 381 2432
Fragments arranged by size:
2432 1371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 227 2992 3046 4530 4695 50
Size: 177 2765 54 1484 165 55
Fragments arranged by size:
2765 1484 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 981 2612 981
Size: 1631 3069
BsrFI r'CCGG_y
Cuts at: 2698 2879 3088 2698
Size: 181 209 4310
Fragments arranged by size:
4310 209 181
BsrGI T'GTAC_A
Cuts at: 1403 1403
Size: 4700
BssSI C'ACGA_G
Cuts at: 2971 3329 2971
Size: 358 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 2077 2077
Size: 4700
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 114 382 1440 114
Size: 268 1058 3374
Fragments arranged by size:
3374 1058 268
BstYI r'GATC_y
Cuts at: 1 679 1243 1437 1526 1725 2192 2215 2550
Size: 678 564 194 89 199 467 23 335
Cuts at: 2550 2796 3808 3894 3906 4252 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 678 564 467 449 346 335 246
199 194 89 86 23 12
BtgI C'CryG_G
Cuts at: 220 1100 220
Size: 880 3820
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 2650 2831 2994 1567
Size: 1083 181 163 3273
Fragments arranged by size:
3273 1083 181 163
BtsI GCAGTG_nn'
Cuts at: 667 1309 1571 1943 667
Size: 642 262 372 3424
Fragments arranged by size:
3424 642 372 262
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 634 2406 3264 634
Size: 1772 858 2070
Fragments arranged by size:
2070 1772 858
EaeI y'GGCC_r
Cuts at: 159 246 576 2285 2459 2850 2877 4222 159
Size: 87 330 1709 174 391 27 1345 637
Fragments arranged by size:
1709 1345 637 391 330 174 87 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 751 1053 1331 2723 2933 751
Size: 302 278 1392 210 2518
Fragments arranged by size:
2518 1392 302 278 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1712 1712
Size: 4700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 1034 1652 2525 2957 3704 3972 711
Size: 323 618 873 432 747 268 1439
Fragments arranged by size:
1439 873 747 618 432 323 268
EcoO109I rG'GnC_Cy
Cuts at: 368 569 991 1839 368
Size: 201 422 848 3229
Fragments arranged by size:
3229 848 422 201
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 2719 441
Size: 2278 2422
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 2134 2382 3404 4383 2134
Size: 248 1022 979 2451
Fragments arranged by size:
2451 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 325 357 671 703 1465 1497 2542
Size: 32 129 32 314 32 762 32 1045
Cuts at: 2542 2572 2574 2604 164
Size: 30 2 30 2260
Fragments arranged by size:
2260 1045 762 314 129 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1287 1739 1287
Size: 452 4248
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1504 3371 3555 1504
Size: 1867 184 2649
Fragments arranged by size:
2649 1867 184
MscI TGG'CCA
Cuts at: 161 248 578 2461 161
Size: 87 330 1883 2400
Fragments arranged by size:
2400 1883 330 87
MslI CAynn'nnrTG
Cuts at: 325 650 1213 2689 2816 325
Size: 325 563 1476 127 2209
Fragments arranged by size:
2209 1476 563 325 127
MspA1I CmG'CkG
Cuts at: 157 1054 1811 2125 2485 3498 3743 157
Size: 897 757 314 360 1013 245 1114
Fragments arranged by size:
1114 1013 897 757 360 314 245
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 4700
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NcoI C'CATG_G
Cuts at: 1100 1100
Size: 4700
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 4700
NsiI A_TGCA'T
Cuts at: 647 1281 647
Size: 634 4066
NspI r_CATG'y
Cuts at: 1194 1753 2253 3160 1194
Size: 559 500 907 2734
Fragments arranged by size:
2734 907 559 500
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 1162 1162
Size: 4700
PfoI T'CCnGG_A
Cuts at: 1008 1008
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 368 569 991 368
Size: 201 422 4077
Fragments arranged by size:
4077 422 201
PshAI GACnn'nnGTC
Cuts at: 236 236
Size: 4700
PsiI TTA'TAA
Cuts at: 362 362
Size: 4700
PstI C_TGCA'G
Cuts at: 1747 2432 1747
Size: 685 4015
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 1054 2125 2485 1054
Size: 1071 360 3269
Fragments arranged by size:
3269 1071 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1714 1714
Size: 4700
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 933 1452 1743 2428 3421 3612 33
Size: 900 519 291 685 993 191 1121
Fragments arranged by size:
1121 993 900 685 519 291 191
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1722 1722
Size: 4700
SmlI C'TyrA_G
Cuts at: 1855 2770 3262 3524 1855
Size: 915 492 262 3031
Fragments arranged by size:
3031 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1194 1753 2253 1194
Size: 559 500 3641
Fragments arranged by size:
3641 559 500
SspI AAT'ATT
Cuts at: 621 621
Size: 4700
StyI C'CwwG_G
Cuts at: 733 1036 1100 1542 1834 2188 733
Size: 303 64 442 292 354 3245
Fragments arranged by size:
3245 442 354 303 292 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 615 1371 2283 2416 2875 3084 615
Size: 756 912 133 459 209 2231
Fragments arranged by size:
2231 912 756 459 209 133
TatI w'GTAC_w
Cuts at: 125 857 950 1403 4215 125
Size: 732 93 453 2812 610
Fragments arranged by size:
2812 732 610 453 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 4217 4249 228
Size: 32 3957 32 679
Fragments arranged by size:
3957 679 32 32
Tth111I GACn'n_nGTC
Cuts at: 553 2497 553
Size: 1944 2756
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 154 586 815 1090 2195 154
Size: 432 229 275 1105 2659
Fragments arranged by size:
2659 1105 432 275 229
XmaI C'CCGG_G
Cuts at: 1720 1720
Size: 4700
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BmgBI BmtI Bpu10I BsiWI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI