Restriction map for
pF1KE1913
(Circular) (Six-base) MAPSORT of: pF1KE1913.seq Check: 2091 from: 1 to: 4463
pF1KE1913 4463 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 254 1501 254
Size: 1247 3216
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 591 2288 2720 3467 3735 345
Size: 246 1697 432 747 268 1073
Fragments arranged by size:
1697 1073 747 432 268 246
AflIII A'CryG_T
Cuts at: 1815 2919 3896 1815
Size: 1104 977 2382
Fragments arranged by size:
2382 1104 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 4463
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 1098 1236 1768 3335 1098
Size: 138 532 1567 2226
Fragments arranged by size:
2226 1567 532 138
ApaI G_GGCC'C
Cuts at: 957 1453 957
Size: 496 3967
ApaLI G'TGCA_C
Cuts at: 121 3233 121
Size: 3112 1351
ApoI r'AATT_y
Cuts at: 1467 3816 4072 1467
Size: 2349 256 1858
Fragments arranged by size:
2349 1858 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 290 834 932 967 1483 290
Size: 544 98 35 516 3270
Fragments arranged by size:
3270 544 516 98 35
BamHI G'GATC_C
Cuts at: 1139 1488 1139
Size: 349 4114
BanI G'GyrC_C
Cuts at: 879 1479 1893 2141 2176 879
Size: 600 414 248 35 3166
Fragments arranged by size:
3166 600 414 248 35
BanII G_rGCy'C
Cuts at: 541 653 957 974 1453 1477 2507 541
Size: 112 304 17 479 24 1030 2497
Fragments arranged by size:
2497 1030 479 304 112 24 17
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BbsI GAAGACnn'nnnn_
Cuts at: 1427 1427
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 1110 1144 135
Size: 34 941 34 3454
Fragments arranged by size:
3454 941 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 593 2361 3122 4463 593
Size: 1768 761 1341 593
Fragments arranged by size:
1768 1341 761 593
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 143 500 143
Size: 357 4106
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 125 553 620 884 957 968 1453 2088 2181
Size: 428 67 264 73 11 485 635 93
Cuts at: 2181 3237 125
Size: 1056 1351
Fragments arranged by size:
1351 1056 635 485 428 264 93 73
67 11
BmrI ACTGGGnnnn_n'
Cuts at: 417 1675 1756 2091 4235 417
Size: 1258 81 335 2144 645
Fragments arranged by size:
2144 1258 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 4463
Bpu10I CC'TnA_GC
Cuts at: 339 339
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1174 1639 2518 3010 3308 736
Size: 438 465 879 492 298 1891
Fragments arranged by size:
1891 879 492 465 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 694 4167 694
Size: 3473 990
BsaAI yAC'GTr
Cuts at: 216 285 1816 216
Size: 69 1531 2863
Fragments arranged by size:
2863 1531 69
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 1388 1894 2142 2844 4340 1388
Size: 506 248 702 1496 1511
Fragments arranged by size:
1511 1496 702 506 248
BsaWI w'CCGG_w
Cuts at: 1652 2173 2851 3125 3272 3683 1652
Size: 521 678 274 147 411 2432
Fragments arranged by size:
2432 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 1444 1447 592
Size: 852 3 3608
Fragments arranged by size:
3608 852 3
BseYI C'CCAG_C
Cuts at: 202 522 977 1888 2646 3223 3719 202
Size: 320 455 911 758 577 496 946
Fragments arranged by size:
946 911 758 577 496 455 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1276 880
Size: 396 4067
BsiEI CG_ry'CG
Cuts at: 104 591 1032 1397 2005 2051 3259 104
Size: 487 441 365 608 46 1208 1308
Fragments arranged by size:
1308 1208 608 487 441 365 46
BsiHKAI G_wGCw'C
Cuts at: 125 974 1477 2255 2445 3237 125
Size: 849 503 778 190 792 1351
Fragments arranged by size:
1351 849 792 778 503 190
Bsp1286I G_dGCh'C
Cuts at: 125 541 553 620 653 884 957 968 974
Size: 416 12 67 33 231 73 11 6
Cuts at: 974 1453 1477 2088 2181 2255 2445 2507 3237
Size: 479 24 611 93 74 190 62 730
Cuts at: 3237 125
Size: 1351
Fragments arranged by size:
1351 730 611 479 416 231 190 93
74 73 67 62 33 24 12 11
6
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 596 3639 596
Size: 3043 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 863 1513 1727 2029 2410 863
Size: 650 214 302 381 2916
Fragments arranged by size:
2916 650 381 302 214
BsrBI CCG'CTC
Cuts at: 50 243 338 809 2755 2809 4293 4458 50
Size: 193 95 471 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 471 193 165 95 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 133 397 2461 2642 2851 133
Size: 264 2064 181 209 1745
Fragments arranged by size:
2064 1745 264 209 181
BssSI C'ACGA_G
Cuts at: 2734 3092 2734
Size: 358 4105
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 4463
BstYI r'GATC_y
Cuts at: 1 1139 1488 1955 1978 2313 2559 3571 3657
Size: 1138 349 467 23 335 246 1012 86
Cuts at: 3657 3669 4015 1
Size: 12 346 449
Fragments arranged by size:
1138 1012 467 449 349 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 323 1026 1182 1391 323
Size: 703 156 209 3395
Fragments arranged by size:
3395 703 209 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 1706 1706
Size: 4463
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 135 758 797 866 1007 1029 1046 1100 1160
Size: 623 39 69 141 22 17 54 60
Cuts at: 1160 1223 1265 1394 2048 2222 2613 2640 3985
Size: 63 42 129 654 174 391 27 1345
Cuts at: 3985 135
Size: 613
Fragments arranged by size:
1345 654 623 613 391 174 141 129
69 63 60 54 42 39 27 22
17
EagI C'GGCC_G
Cuts at: 1029 1394 2048 1029
Size: 365 654 3444
Fragments arranged by size:
3444 654 365
EarI CTCTTCn'nnn_
Cuts at: 276 364 1397 2486 2696 276
Size: 88 1033 1089 210 2043
Fragments arranged by size:
2043 1089 1033 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 451 2671 2981 3127 4273 451
Size: 2220 310 146 1146 641
Fragments arranged by size:
2220 1146 641 310 146
EcoICRI GAG'CTC
Cuts at: 972 1475 972
Size: 503 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 345 591 2288 2720 3467 3735 259
Size: 86 246 1697 432 747 268 987
Fragments arranged by size:
1697 987 747 432 268 246 86
EcoO109I rG'GnC_Cy
Cuts at: 344 777 954 1449 1450 1602 344
Size: 433 177 495 1 152 3205
Fragments arranged by size:
3205 495 433 177 152 1
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FseI GG_CCGG'CC
Cuts at: 137 137
Size: 4463
FspI TGC'GCA
Cuts at: 859 2244 859
Size: 1385 3078
HaeII r_GCGC'y
Cuts at: 727 1897 2145 3167 4146 727
Size: 1170 248 1022 979 1044
Fragments arranged by size:
1170 1044 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 1420 1452 2305 2335 2337 2367 1348
Size: 32 40 32 853 30 2 30 3444
Fragments arranged by size:
3444 853 40 32 32 30 30 2
HincII GTy'rAC
Cuts at: 873 1502 873
Size: 629 3834
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1418 3134 3318 1418
Size: 1716 184 2563
Fragments arranged by size:
2563 1716 184
MscI TGG'CCA
Cuts at: 760 1048 1102 2224 760
Size: 288 54 1122 2999
Fragments arranged by size:
2999 1122 288 54
MslI CAynn'nnrTG
Cuts at: 301 643 1072 1267 2452 2579 301
Size: 342 429 195 1185 127 2185
Fragments arranged by size:
2185 1185 429 342 195 127
MspA1I CmG'CkG
Cuts at: 206 237 510 681 1184 1574 1888 2248 3261
Size: 31 273 171 503 390 314 360 1013
Cuts at: 3261 3506 206
Size: 245 1163
Fragments arranged by size:
1163 1013 503 390 360 314 273 245
171 31
NaeI GCC'GGC
Cuts at: 135 399 2644 135
Size: 264 2245 1954
Fragments arranged by size:
2245 1954 264
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NcoI C'CATG_G
Cuts at: 323 323
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 133 397 2642 133
Size: 264 2245 1954
Fragments arranged by size:
2245 1954 264
NspI r_CATG'y
Cuts at: 1071 1516 2016 2923 1071
Size: 445 500 907 2611
Fragments arranged by size:
2611 907 500 445
PasI CC'CwG_GG
Cuts at: 1446 1446
Size: 4463
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PflMI CCAn_nnn'nTGG
Cuts at: 167 522 681 684 167
Size: 355 159 3 3946
Fragments arranged by size:
3946 355 159 3
PfoI T'CCnGG_A
Cuts at: 455 1229 455
Size: 774 3689
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1312 1344 3602 3634 1312
Size: 32 2258 32 2141
Fragments arranged by size:
2258 2141 32 32
PpuMI rG'GwC_Cy
Cuts at: 777 777
Size: 4463
PspOMI G'GGCC_C
Cuts at: 953 1449 953
Size: 496 3967
PspXI vC'TCGA_Gb
Cuts at: 290 290
Size: 4463
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1108 1140 1108
Size: 32 4431
PstI C_TGCA'G
Cuts at: 500 1510 2195 500
Size: 1010 685 2768
Fragments arranged by size:
2768 1010 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 237 510 681 1888 2248 237
Size: 273 171 1207 360 2452
Fragments arranged by size:
2452 1207 360 273 171
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 974 1477 974
Size: 503 3960
SacII CC_GC'GG
Cuts at: 1185 1185
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 500 1510 500
Size: 1010 3453
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SexAI A'CCwGG_T
Cuts at: 788 1358 788
Size: 570 3893
SfcI C'TryA_G
Cuts at: 33 496 1506 2191 3184 3375 33
Size: 463 1010 685 993 191 1121
Fragments arranged by size:
1121 1010 993 685 463 191
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 836 934 1485 836
Size: 98 551 3814
Fragments arranged by size:
3814 551 98
SmlI C'TyrA_G
Cuts at: 290 751 1189 1618 2533 3025 3287 290
Size: 461 438 429 915 492 262 1466
Fragments arranged by size:
1466 915 492 461 438 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StuI AGG'CCT
Cuts at: 268 366 1285 268
Size: 98 919 3446
Fragments arranged by size:
3446 919 98
StyI C'CwwG_G
Cuts at: 323 413 626 1454 1597 1951 323
Size: 90 213 828 143 354 2835
Fragments arranged by size:
2835 828 354 213 143 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 639 991 2046 2179 2638 2847 441
Size: 198 352 1055 133 459 209 2057
Fragments arranged by size:
2057 1055 459 352 209 198 133
TatI w'GTAC_w
Cuts at: 3978 3978
Size: 4463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 462 494 3980 4012 462
Size: 32 3486 32 913
Fragments arranged by size:
3486 913 32 32
Tth111I GACn'n_nGTC
Cuts at: 331 2260 331
Size: 1929 2534
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 1002 1080 1958 678
Size: 324 78 878 3183
Fragments arranged by size:
3183 878 324 78
XhoI C'TCGA_G
Cuts at: 290 290
Size: 4463
XmaI C'CCGG_G
Cuts at: 834 932 1483 834
Size: 98 551 3814
Fragments arranged by size:
3814 551 98
XmnI GAAnn'nnTTC
Cuts at: 604 604
Size: 4463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI