Restriction map for
pF1KE1860
(Circular) (Six-base) MAPSORT of: pF1KE1860.seq Check: 5329 from: 1 to: 4253
pF1KE1860 4253 bp
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 273 603 273
Size: 330 3923
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 2078 2510 3257 3525 189
Size: 1889 432 747 268 917
Fragments arranged by size:
1889 917 747 432 268
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1072 1072
Size: 4253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 1224 1256 490
Size: 32 702 32 3487
Fragments arranged by size:
3487 702 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1227 1558 3125 1227
Size: 331 1567 2355
Fragments arranged by size:
2355 1567 331
ApaI G_GGCC'C
Cuts at: 461 754 777 851 461
Size: 293 23 74 3863
Fragments arranged by size:
3863 293 74 23
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 1273 1273
Size: 4253
BamHI G'GATC_C
Cuts at: 1278 1278
Size: 4253
BanI G'GyrC_C
Cuts at: 1269 1683 1931 1966 1269
Size: 414 248 35 3556
Fragments arranged by size:
3556 414 248 35
BanII G_rGCy'C
Cuts at: 461 754 777 851 1267 2297 461
Size: 293 23 74 416 1030 2417
Fragments arranged by size:
2417 1030 416 293 74 23
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 1067 1067
Size: 4253
BbvCI CC'TCA_GC
Cuts at: 1228 1228
Size: 4253
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglI GCCn_nnn'nGGC
Cuts at: 424 439 919 424
Size: 15 480 3758
Fragments arranged by size:
3758 480 15
BglII A'GATC_T
Cuts at: 218 1768 3805 218
Size: 1550 2037 666
Fragments arranged by size:
2037 1550 666
BlpI GC'TnA_GC
Cuts at: 1365 1365
Size: 4253
Bme1580I G_kGCm'C
Cuts at: 435 461 754 777 851 1878 1971 3027 435
Size: 26 293 23 74 1027 93 1056 1661
Fragments arranged by size:
1661 1056 1027 293 93 74 26 23
BmrI ACTGGGnnnn_n'
Cuts at: 402 1465 1546 1881 4025 402
Size: 1063 81 335 2144 630
Fragments arranged by size:
2144 1063 630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 826 855 2620 826
Size: 29 1765 2459
Fragments arranged by size:
2459 1765 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 821 1104 629
Size: 192 283 3778
Fragments arranged by size:
3778 283 192
Bpu10I CC'TnA_GC
Cuts at: 1228 1228
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 2800 3098 1429
Size: 879 492 298 2584
Fragments arranged by size:
2584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 579 1023 1090 3957 579
Size: 444 67 2867 875
Fragments arranged by size:
2867 875 444 67
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 1684 1932 2634 4130 1684
Size: 248 702 1496 1807
Fragments arranged by size:
1807 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 719 749 490
Size: 30 199 30 3994
Fragments arranged by size:
3994 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 632 847 1000 529
Size: 103 215 153 3782
Fragments arranged by size:
3782 215 153 103
BseYI C'CCAG_C
Cuts at: 471 607 687 769 1114 1678 2436 3013 3509
Size: 136 80 82 345 564 758 577 496
Cuts at: 3509 471
Size: 1215
Fragments arranged by size:
1215 758 577 564 496 345 136 82
80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1092 710
Size: 382 3871
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3049 104
Size: 1691 46 1208 1308
Fragments arranged by size:
1691 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 560 1267 2045 2235 3027 560
Size: 707 778 190 792 1786
Fragments arranged by size:
1786 792 778 707 190
BsmBI CGTCTCn'nnnn_
Cuts at: 109 109
Size: 4253
Bsp1286I G_dGCh'C
Cuts at: 435 461 560 754 777 851 1267 1878 1971
Size: 26 99 194 23 74 416 611 93
Cuts at: 1971 2045 2235 2297 3027 435
Size: 74 190 62 730 1661
Fragments arranged by size:
1661 730 611 416 194 190 99 93
74 74 62 26 23
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 273 603 1303 1517 1819 2200 273
Size: 330 700 214 302 381 2326
Fragments arranged by size:
2326 700 381 330 302 214
BsrBI CCG'CTC
Cuts at: 50 2545 2599 4083 4248 50
Size: 2495 54 1484 165 55
Fragments arranged by size:
2495 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2165 2165
Size: 4253
BsrFI r'CCGG_y
Cuts at: 418 2251 2432 2641 418
Size: 1833 181 209 2030
Fragments arranged by size:
2030 1833 209 181
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 796 1630 796
Size: 834 3419
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstEII G'GTnAC_C
Cuts at: 1083 1083
Size: 4253
BstYI r'GATC_y
Cuts at: 1 218 1278 1745 1768 2103 2349 3361 3447
Size: 217 1060 467 23 335 246 1012 86
Cuts at: 3447 3459 3805 1
Size: 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 246 217
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 840 840
Size: 4253
BtgI C'CryG_G
Cuts at: 233 233
Size: 4253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 186 2203 2384 2547 186
Size: 2017 181 163 1892
Fragments arranged by size:
2017 1892 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1496
Size: 4253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 927 962 927
Size: 35 4218
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 236 425 785 1838 2012 2403 2430 3775 236
Size: 189 360 1053 174 391 27 1345 714
Fragments arranged by size:
1345 1053 714 391 360 189 174 27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 896 1160 2276 2486 896
Size: 264 1116 210 2663
Fragments arranged by size:
2663 1116 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 629 821 1104 2078 2510 3257 3525 189
Size: 440 192 283 974 432 747 268 917
Fragments arranged by size:
974 917 747 440 432 283 268 192
EcoNI CCTnn'n_nnAGG
Cuts at: 644 1059 644
Size: 415 3838
EcoO109I rG'GnC_Cy
Cuts at: 193 375 458 631 649 684 750 751 773
Size: 182 83 173 18 35 66 1 22
Cuts at: 773 774 847 1392 193
Size: 1 73 545 3054
Fragments arranged by size:
3054 545 182 173 83 73 66 35
22 18 1 1
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 570 1687 1935 2957 3936 570
Size: 1117 248 1022 979 887
Fragments arranged by size:
1117 1022 979 887 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2095 2125 2127 2157 2095
Size: 30 2 30 4191
Fragments arranged by size:
4191 30 30 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 533 2924 3108 425
Size: 108 2391 184 1570
Fragments arranged by size:
2391 1570 184 108
MscI TGG'CCA
Cuts at: 427 2014 427
Size: 1587 2666
MslI CAynn'nnrTG
Cuts at: 145 2242 2369 145
Size: 2097 127 2029
Fragments arranged by size:
2097 2029 127
MspA1I CmG'CkG
Cuts at: 607 691 1364 1678 2038 3051 3296 607
Size: 84 673 314 360 1013 245 1564
Fragments arranged by size:
1564 1013 673 360 314 245 84
NaeI GCC'GGC
Cuts at: 420 2434 420
Size: 2014 2239
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NgoMIV G'CCGG_C
Cuts at: 418 2432 418
Size: 2014 2239
NspI r_CATG'y
Cuts at: 1306 1806 2713 1306
Size: 500 907 2846
Fragments arranged by size:
2846 907 500
PasI CC'CwG_GG
Cuts at: 1034 1034
Size: 4253
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 285 285
Size: 4253
PfoI T'CCnGG_A
Cuts at: 1220 1220
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 490 522 3392 3424 490
Size: 32 2870 32 1319
Fragments arranged by size:
2870 1319 32 32
PpuMI rG'GwC_Cy
Cuts at: 193 631 649 684 193
Size: 438 18 35 3762
Fragments arranged by size:
3762 438 35 18
PspOMI G'GGCC_C
Cuts at: 457 750 773 847 457
Size: 293 23 74 3863
Fragments arranged by size:
3863 293 74 23
PstI C_TGCA'G
Cuts at: 696 1300 1985 696
Size: 604 685 2964
Fragments arranged by size:
2964 685 604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 691 1678 2038 691
Size: 987 360 2906
Fragments arranged by size:
2906 987 360
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SanDI GG'GwC_CC
Cuts at: 193 684 193
Size: 491 3762
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SexAI A'CCwGG_T
Cuts at: 484 484
Size: 4253
SfcI C'TryA_G
Cuts at: 33 692 1138 1296 1981 2974 3165 33
Size: 659 446 158 685 993 191 1121
Fragments arranged by size:
1121 993 685 659 446 191 158
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 1408 2323 2815 3077 1408
Size: 915 492 262 2584
Fragments arranged by size:
2584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StuI AGG'CCT
Cuts at: 202 782 838 202
Size: 580 56 3617
Fragments arranged by size:
3617 580 56
StyI C'CwwG_G
Cuts at: 962 1060 1387 1741 962
Size: 98 327 354 3474
Fragments arranged by size:
3474 354 327 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 1836 1969 2428 2637 480
Size: 1356 133 459 209 2096
Fragments arranged by size:
2096 1356 459 209 133
TatI w'GTAC_w
Cuts at: 3768 3768
Size: 4253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 3770 3802 719
Size: 32 3019 32 1170
Fragments arranged by size:
3019 1170 32 32
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1748 1748
Size: 4253
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI