Restriction map for
pF1KE1851
(Circular) (Six-base) MAPSORT of: pF1KE1851.seq Check: 3051 from: 1 to: 4223
pF1KE1851 4223 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 977 1261 977
Size: 284 3939
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2480 3227 3495 2048
Size: 432 747 268 2776
Fragments arranged by size:
2776 747 432 268
AfeI AGC'GCT
Cuts at: 1138 1138
Size: 4223
AflIII A'CryG_T
Cuts at: 562 1575 2679 3656 562
Size: 1013 1104 977 1129
Fragments arranged by size:
1129 1104 1013 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 214
Size: 32 4191
AlwNI CAG_nnn'CTG
Cuts at: 1528 3095 1528
Size: 1567 2656
ApaI G_GGCC'C
Cuts at: 437 595 437
Size: 158 4065
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 1227 3576 3832 1227
Size: 2349 256 1618
Fragments arranged by size:
2349 1618 256
AscI GG'CGCG_CC
Cuts at: 1044 1044
Size: 4223
AseI AT'TA_AT
Cuts at: 20 20
Size: 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 277 586 1243 277
Size: 309 657 3257
Fragments arranged by size:
3257 657 309
BamHI G'GATC_C
Cuts at: 338 1248 338
Size: 910 3313
BanI G'GyrC_C
Cuts at: 862 1239 1653 1901 1936 862
Size: 377 414 248 35 3149
Fragments arranged by size:
3149 414 377 248 35
BanII G_rGCy'C
Cuts at: 116 284 437 595 1237 2267 116
Size: 168 153 158 642 1030 2072
Fragments arranged by size:
2072 1030 642 168 158 153
BbeI G_GCGC'C
Cuts at: 866 1657 1905 866
Size: 791 248 3184
Fragments arranged by size:
3184 791 248
BbsI GAAGACnn'nnnn_
Cuts at: 944 944
Size: 4223
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BglI GCCn_nnn'nGGC
Cuts at: 590 647 590
Size: 57 4166
BglII A'GATC_T
Cuts at: 1738 3775 1738
Size: 2037 2186
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 437 595 1027 1848 1941 2997 437
Size: 158 432 821 93 1056 1663
Fragments arranged by size:
1663 1056 821 432 158 93
BmgBI CAC'GTC
Cuts at: 1170 1170
Size: 4223
BmrI ACTGGGnnnn_n'
Cuts at: 278 615 1435 1516 1851 3995 278
Size: 337 820 81 335 2144 506
Fragments arranged by size:
2144 820 506 337 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 949 413
Size: 536 3687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2278 2770 3068 1399
Size: 879 492 298 2554
Fragments arranged by size:
2554 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 563 800 1007 1576 563
Size: 237 207 569 3210
Fragments arranged by size:
3210 569 237 207
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 315 863 1017 1654 1902 2604 4100 315
Size: 548 154 637 248 702 1496 438
Fragments arranged by size:
1496 702 637 548 438 248 154
BsaWI w'CCGG_w
Cuts at: 398 1412 1933 2611 2885 3032 3443 398
Size: 1014 521 678 274 147 411 1178
Fragments arranged by size:
1178 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 748 778 214
Size: 30 504 30 3659
Fragments arranged by size:
3659 504 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 222 222
Size: 4223
BseYI C'CCAG_C
Cuts at: 134 1648 2406 2983 3479 134
Size: 1514 758 577 496 878
Fragments arranged by size:
1514 878 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 543 987 1131 1170 1189 328
Size: 215 444 144 39 19 3362
Fragments arranged by size:
3362 444 215 144 39 19
BsiEI CG_ry'CG
Cuts at: 104 639 651 734 852 1765 1811 3019 104
Size: 535 12 83 118 913 46 1208 1308
Fragments arranged by size:
1308 1208 913 535 118 83 46 12
BsiHKAI G_wGCw'C
Cuts at: 1237 2015 2205 2997 1237
Size: 778 190 792 2463
Fragments arranged by size:
2463 792 778 190
BsiWI C'GTAC_G
Cuts at: 727 727
Size: 4223
Bsp1286I G_dGCh'C
Cuts at: 116 284 437 595 1027 1237 1848 1941 2015
Size: 168 153 158 432 210 611 93 74
Cuts at: 2015 2205 2267 2997 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 730 611 432 210 190 168 158
153 93 74 62
BspEI T'CCGG_A
Cuts at: 398 3443 398
Size: 3045 1178
BspHI T'CATG_A
Cuts at: 698 3399 698
Size: 2701 1522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 695 1273 1487 1789 2170 251
Size: 444 578 214 302 381 2304
Fragments arranged by size:
2304 578 444 381 302 214
BsrBI CCG'CTC
Cuts at: 50 169 2515 2569 4053 4218 50
Size: 119 2346 54 1484 165 55
Fragments arranged by size:
2346 1484 165 119 55 54
BsrDI GCAATG_nn'
Cuts at: 2135 2135
Size: 4223
BsrFI r'CCGG_y
Cuts at: 572 2221 2402 2611 572
Size: 1649 181 209 2184
Fragments arranged by size:
2184 1649 209 181
BssHII G'CGCG_C
Cuts at: 526 825 1044 526
Size: 299 219 3705
Fragments arranged by size:
3705 299 219
BssSI C'ACGA_G
Cuts at: 1207 2494 2852 1207
Size: 1287 358 2578
Fragments arranged by size:
2578 1287 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1600 1600
Size: 4223
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 510 510
Size: 4223
BstYI r'GATC_y
Cuts at: 1 338 1248 1715 1738 2073 2319 3331 3417
Size: 337 910 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 910 467 449 346 337 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 768 830 888 107
Size: 661 62 58 3442
Fragments arranged by size:
3442 661 62 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 851 2173 2354 2517 851
Size: 1322 181 163 2557
Fragments arranged by size:
2557 1322 181 163
BtsI GCAGTG_nn'
Cuts at: 204 422 1466 204
Size: 218 1044 2961
Fragments arranged by size:
2961 1044 218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 180 215 180
Size: 35 4188
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DraIII CAC_nnn'GTG
Cuts at: 351 351
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 164 201 636 648 1808 1982 2373 2400 3745
Size: 37 435 12 1160 174 391 27 1345
Cuts at: 3745 164
Size: 642
Fragments arranged by size:
1345 1160 642 435 391 174 37 27
12
EagI C'GGCC_G
Cuts at: 636 648 1808 636
Size: 12 1160 3051
Fragments arranged by size:
3051 1160 12
EarI CTCTTCn'nnn_
Cuts at: 448 649 2246 2456 448
Size: 201 1597 210 2215
Fragments arranged by size:
2215 1597 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 492 928 2431 2741 2887 4033 492
Size: 436 1503 310 146 1146 682
Fragments arranged by size:
1503 1146 682 436 310 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 949 2048 2480 3227 3495 413
Size: 536 1099 432 747 268 1141
Fragments arranged by size:
1141 1099 747 536 432 268
EcoO109I rG'GnC_Cy
Cuts at: 433 434 591 592 684 1362 433
Size: 1 157 1 92 678 3294
Fragments arranged by size:
3294 678 157 92 1 1
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 4223
FspI TGC'GCA
Cuts at: 796 2004 796
Size: 1208 3015
FspAI rTGC'GCAy
Cuts at: 796 796
Size: 4223
HaeII r_GCGC'y
Cuts at: 124 163 866 1140 1657 1905 2927 3906 124
Size: 39 703 274 517 248 1022 979 441
Fragments arranged by size:
1022 979 703 517 441 274 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 850 882 2065 2095 2097 2127 850
Size: 32 1183 30 2 30 2946
Fragments arranged by size:
2946 1183 32 30 30 2
HincII GTy'rAC
Cuts at: 459 1262 459
Size: 803 3420
KasI G'GCGC_C
Cuts at: 862 1653 1901 862
Size: 791 248 3184
Fragments arranged by size:
3184 791 248
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2894 3078 2894
Size: 184 4039
MscI TGG'CCA
Cuts at: 1984 1984
Size: 4223
MslI CAynn'nnrTG
Cuts at: 2212 2339 2212
Size: 127 4096
MspA1I CmG'CkG
Cuts at: 294 312 410 890 1013 1175 1334 1648 2008
Size: 18 98 480 123 162 159 314 360
Cuts at: 2008 3021 3266 294
Size: 1013 245 1251
Fragments arranged by size:
1251 1013 480 360 314 245 162 159
123 98 18
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 863 1654 1902 863
Size: 791 248 3184
Fragments arranged by size:
3184 791 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NspI r_CATG'y
Cuts at: 1276 1776 2683 1276
Size: 500 907 2816
Fragments arranged by size:
2816 907 500
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PflMI CCAn_nnn'nTGG
Cuts at: 522 522
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PmlI CAC'GTG
Cuts at: 563 800 563
Size: 237 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 3362 3394 214
Size: 32 3116 32 1043
Fragments arranged by size:
3116 1043 32 32
PpuMI rG'GwC_Cy
Cuts at: 684 684
Size: 4223
PspOMI G'GGCC_C
Cuts at: 433 591 433
Size: 158 4065
PspXI vC'TCGA_Gb
Cuts at: 277 277
Size: 4223
PstI C_TGCA'G
Cuts at: 293 368 619 1270 1955 293
Size: 75 251 651 685 2561
Fragments arranged by size:
2561 685 651 251 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 294 1175 1648 2008 294
Size: 881 473 360 2509
Fragments arranged by size:
2509 881 473 360
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SacII CC_GC'GG
Cuts at: 891 891
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 289 364 615 1266 1951 2944 3135 33
Size: 256 75 251 651 685 993 191 1121
Fragments arranged by size:
1121 993 685 651 256 251 191 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 590 590
Size: 4223
SfoI GGC'GCC
Cuts at: 864 1655 1903 864
Size: 791 248 3184
Fragments arranged by size:
3184 791 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 588 1245 588
Size: 657 3566
SmlI C'TyrA_G
Cuts at: 277 1378 2293 2785 3047 277
Size: 1101 915 492 262 1453
Fragments arranged by size:
1453 1101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
StyI C'CwwG_G
Cuts at: 107 1357 1711 107
Size: 1250 354 2619
Fragments arranged by size:
2619 1250 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 1806 1939 2398 2607 529
Size: 1277 133 459 209 2145
Fragments arranged by size:
2145 1277 459 209 133
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 748 760 780 792 3740 3772 748
Size: 12 20 12 2948 32 1199
Fragments arranged by size:
2948 1199 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 973 2020 973
Size: 1047 3176
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XhoI C'TCGA_G
Cuts at: 277 277
Size: 4223
XmaI C'CCGG_G
Cuts at: 586 1243 586
Size: 657 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsmI BsmBI BsrGI
BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI