Restriction map for
pF1KE1842
(Circular) (Six-base) MAPSORT of: pF1KE1842.seq Check: 1778 from: 1 to: 4187
pF1KE1842 4187 bp
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 170 252 731 773 170
Size: 82 479 42 3584
Fragments arranged by size:
3584 479 82 42
AatII G_ACGT'C
Cuts at: 289 289
Size: 4187
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2444 3191 3459 2012
Size: 432 747 268 2740
Fragments arranged by size:
2740 747 432 268
AflIII A'CryG_T
Cuts at: 1539 2643 3620 1539
Size: 1104 977 2106
Fragments arranged by size:
2106 1104 977
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AleI CACnn'nnGTG
Cuts at: 799 799
Size: 4187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 178 178
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 1492 3059 1492
Size: 1567 2620
ApaI G_GGCC'C
Cuts at: 223 397 451 488 853 223
Size: 174 54 37 365 3557
Fragments arranged by size:
3557 365 174 54 37
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AscI GG'CGCG_CC
Cuts at: 437 437
Size: 4187
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 209 432 469 1207 209
Size: 223 37 738 3189
Fragments arranged by size:
3189 738 223 37
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 457 561 832 877 1203 1617 1865 1900 457
Size: 104 271 45 326 414 248 35 2744
Fragments arranged by size:
2744 414 326 271 248 104 45 35
BanII G_rGCy'C
Cuts at: 172 223 397 451 488 542 599 853 1153
Size: 51 174 54 37 54 57 254 300
Cuts at: 1153 1201 2231 172
Size: 48 1030 2128
Fragments arranged by size:
2128 1030 300 254 174 57 54 54
51 48 37
BbeI G_GCGC'C
Cuts at: 461 565 881 1621 1869 461
Size: 104 316 740 248 2779
Fragments arranged by size:
2779 740 316 248 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 969 1003 969
Size: 34 4153
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 584 1707 584
Size: 1123 3064
BglI GCCn_nnn'nGGC
Cuts at: 385 401 455 848 385
Size: 16 54 393 3724
Fragments arranged by size:
3724 393 54 16
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1099 1299 1099
Size: 200 3987
Bme1580I G_kGCm'C
Cuts at: 223 397 451 488 806 853 1812 1905 2961
Size: 174 54 37 318 47 959 93 1056
Cuts at: 2961 223
Size: 1449
Fragments arranged by size:
1449 1056 959 318 174 93 54 47
37
BmrI ACTGGGnnnn_n'
Cuts at: 1399 1480 1815 3959 1399
Size: 81 335 2144 1627
Fragments arranged by size:
2144 1627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1072 556
Size: 516 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1363 2242 2734 3032 850
Size: 513 879 492 298 2005
Fragments arranged by size:
2005 879 513 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3891 3891
Size: 4187
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 286 458 562 878 1618 1866 2568 4064 286
Size: 172 104 316 740 248 702 1496 409
Fragments arranged by size:
1496 740 702 409 316 248 172 104
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 529
Size: 30 4157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 619
Size: 4187
BseYI C'CCAG_C
Cuts at: 184 261 805 873 1612 2370 2947 3443 184
Size: 77 544 68 739 758 577 496 928
Fragments arranged by size:
928 758 739 577 544 496 77 68
BsiEI CG_ry'CG
Cuts at: 104 119 320 389 512 577 1729 1775 2983
Size: 15 201 69 123 65 1152 46 1208
Cuts at: 2983 104
Size: 1308
Fragments arranged by size:
1308 1208 1152 201 123 69 65 46
15
BsiHKAI G_wGCw'C
Cuts at: 281 1153 1201 1979 2169 2961 281
Size: 872 48 778 190 792 1507
Fragments arranged by size:
1507 872 792 778 190 48
BsmBI CGTCTCn'nnnn_
Cuts at: 277 1027 277
Size: 750 3437
Bsp1286I G_dGCh'C
Cuts at: 172 223 281 397 451 488 542 599 806
Size: 51 58 116 54 37 54 57 207
Cuts at: 806 853 1153 1201 1812 1905 1979 2169 2231
Size: 47 300 48 611 93 74 190 62
Cuts at: 2231 2961 172
Size: 730 1398
Fragments arranged by size:
1398 730 611 300 207 190 116 93
74 62 58 57 54 54 51 48
47 37
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 252 731 773 1237 1451 1753 2134 170
Size: 82 479 42 464 214 302 381 2223
Fragments arranged by size:
2223 479 464 381 302 214 82 42
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 362 514 564 634 838 2185 2366 2575 362
Size: 152 50 70 204 1347 181 209 1974
Fragments arranged by size:
1974 1347 209 204 181 152 70 50
BssHII G'CGCG_C
Cuts at: 437 860 437
Size: 423 3764
BssSI C'ACGA_G
Cuts at: 280 2458 2816 280
Size: 2178 358 1651
Fragments arranged by size:
2178 1651 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1047 1564 1047
Size: 517 3670
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstEII G'GTnAC_C
Cuts at: 775 775
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 624 624
Size: 4187
BstYI r'GATC_y
Cuts at: 1 1212 1679 1702 2037 2283 3295 3381 3393
Size: 1211 467 23 335 246 1012 86 12
Cuts at: 3393 3739 1
Size: 346 449
Fragments arranged by size:
1211 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 139 389 398 443 1166 107
Size: 32 250 9 45 723 3128
Fragments arranged by size:
3128 723 250 45 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 972 2137 2318 2481 972
Size: 1165 181 163 2678
Fragments arranged by size:
2678 1165 181 163
BtsI GCAGTG_nn'
Cuts at: 741 1430 741
Size: 689 3498
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 116 317 341 386 509 574 632 710 747
Size: 201 24 45 123 65 58 78 37
Cuts at: 747 1772 1946 2337 2364 3709 116
Size: 1025 174 391 27 1345 594
Fragments arranged by size:
1345 1025 594 391 201 174 123 78
65 58 45 37 27 24
EagI C'GGCC_G
Cuts at: 116 317 386 509 574 1772 116
Size: 201 69 123 65 1198 2531
Fragments arranged by size:
2531 1198 201 123 69 65
EarI CTCTTCn'nnn_
Cuts at: 646 2210 2420 646
Size: 1564 210 2413
Fragments arranged by size:
2413 1564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 1123 2395 2705 2851 3997 148
Size: 975 1272 310 146 1146 338
Fragments arranged by size:
1272 1146 975 338 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1199 1151
Size: 48 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1072 2012 2444 3191 3459 556
Size: 516 940 432 747 268 1284
Fragments arranged by size:
1284 940 747 516 432 268
EcoO109I rG'GnC_Cy
Cuts at: 220 394 448 485 850 1326 220
Size: 174 54 37 365 476 3081
Fragments arranged by size:
3081 476 365 174 54 37
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FseI GG_CCGG'CC
Cuts at: 638 638
Size: 4187
FspI TGC'GCA
Cuts at: 997 1081 1968 997
Size: 84 887 3216
Fragments arranged by size:
3216 887 84
HaeII r_GCGC'y
Cuts at: 194 305 461 565 571 631 881 1621 1869
Size: 111 156 104 6 60 250 740 248
Cuts at: 1869 2891 3870 194
Size: 1022 979 511
Fragments arranged by size:
1022 979 740 511 250 248 156 111
104 60 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2029 2059 2061 2091 2029
Size: 30 2 30 4125
Fragments arranged by size:
4125 30 30 2
HincII GTy'rAC
Cuts at: 1226 1226
Size: 4187
KasI G'GCGC_C
Cuts at: 457 561 877 1617 1865 457
Size: 104 316 740 248 2779
Fragments arranged by size:
2779 740 316 248 104
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3042 2858
Size: 184 4003
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 799 991 2176 2303 799
Size: 192 1185 127 2683
Fragments arranged by size:
2683 1185 192 127
MspA1I CmG'CkG
Cuts at: 141 261 445 579 624 914 1098 1140 1298
Size: 120 184 134 45 290 184 42 158
Cuts at: 1298 1612 1972 2985 3230 141
Size: 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 360 314 290 245 184 184
158 134 120 45 42
NaeI GCC'GGC
Cuts at: 364 516 566 636 840 2368 364
Size: 152 50 70 204 1528 2183
Fragments arranged by size:
2183 1528 204 152 70 50
NarI GG'CG_CC
Cuts at: 458 562 878 1618 1866 458
Size: 104 316 740 248 2779
Fragments arranged by size:
2779 740 316 248 104
NcoI C'CATG_G
Cuts at: 107 1166 107
Size: 1059 3128
NgoMIV G'CCGG_C
Cuts at: 362 514 564 634 838 2366 362
Size: 152 50 70 204 1528 2183
Fragments arranged by size:
2183 1528 204 152 70 50
NotI GC'GGCC_GC
Cuts at: 509 574 509
Size: 65 4122
NspI r_CATG'y
Cuts at: 1240 1740 2647 1240
Size: 500 907 2780
Fragments arranged by size:
2780 907 500
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PspOMI G'GGCC_C
Cuts at: 219 393 447 484 849 219
Size: 174 54 37 365 3557
Fragments arranged by size:
3557 365 174 54 37
PstI C_TGCA'G
Cuts at: 167 728 770 1234 1919 167
Size: 561 42 464 685 2435
Fragments arranged by size:
2435 685 561 464 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 624 1098 1140 1612 1972 624
Size: 474 42 472 360 2839
Fragments arranged by size:
2839 474 472 360 42
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1153 1201 1153
Size: 48 4139
SacII CC_GC'GG
Cuts at: 142 446 142
Size: 304 3883
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 163 724 766 1230 1915 2908 3099 33
Size: 130 561 42 464 685 993 191 1121
Fragments arranged by size:
1121 993 685 561 464 191 130 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 385 848 385
Size: 463 3724
SfoI GGC'GCC
Cuts at: 459 563 879 1619 1867 459
Size: 104 316 740 248 2779
Fragments arranged by size:
2779 740 316 248 104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SgrAI Cr'CCGG_yG
Cuts at: 564 564
Size: 4187
SmaI CCC'GGG
Cuts at: 211 434 471 1209 211
Size: 223 37 738 3189
Fragments arranged by size:
3189 738 223 37
SmlI C'TyrA_G
Cuts at: 865 1342 2257 2749 3011 865
Size: 477 915 492 262 2041
Fragments arranged by size:
2041 915 492 477 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StyI C'CwwG_G
Cuts at: 107 380 1154 1166 1321 1675 107
Size: 273 774 12 155 354 2619
Fragments arranged by size:
2619 774 354 273 155 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 271 303 3704 3736 271
Size: 32 3401 32 722
Fragments arranged by size:
3401 722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 936 1682 387
Size: 549 746 2892
Fragments arranged by size:
2892 746 549
XmaI C'CCGG_G
Cuts at: 209 432 469 1207 209
Size: 223 37 738 3189
Fragments arranged by size:
3189 738 223 37
ZraI GAC'GTC
Cuts at: 287 287
Size: 4187
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AvrII BaeI BbsI BbvCI
BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI