Restriction map for
pF1KE1808
(Circular) (Six-base) MAPSORT of: pF1KE1808.seq Check: 5292 from: 1 to: 4088
pF1KE1808 4088 bp
With 182 enzymes: SgfI *
July 8, 2015 10:32 ..
AccI GT'mk_AC
Cuts at: 311 874 1126 311
Size: 563 252 3273
Fragments arranged by size:
3273 563 252
Acc65I G'GTAC_C
Cuts at: 524 1104 524
Size: 580 3508
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
AflIII A'CryG_T
Cuts at: 758 1440 2544 3521 758
Size: 682 1104 977 1325
Fragments arranged by size:
1325 1104 977 682
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AlwNI CAG_nnn'CTG
Cuts at: 1393 2960 1393
Size: 1567 2521
ApaLI G'TGCA_C
Cuts at: 211 2858 211
Size: 2647 1441
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 1108 1108
Size: 4088
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 497 524 1037 1104 1518 1766 1801 497
Size: 27 513 67 414 248 35 2784
Fragments arranged by size:
2784 513 414 248 67 35 27
BanII G_rGCy'C
Cuts at: 582 1102 2132 582
Size: 520 1030 2538
Fragments arranged by size:
2538 1030 520
BbeI G_GCGC'C
Cuts at: 1041 1522 1770 1041
Size: 481 248 3359
Fragments arranged by size:
3359 481 248
BbsI GAAGACnn'nnnn_
Cuts at: 693 693
Size: 4088
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 1200 1200
Size: 4088
Bme1580I G_kGCm'C
Cuts at: 215 570 1713 1806 2862 215
Size: 355 1143 93 1056 1441
Fragments arranged by size:
1441 1143 1056 355 93
BmrI ACTGGGnnnn_n'
Cuts at: 225 232 1300 1381 1716 3860 225
Size: 7 1068 81 335 2144 453
Fragments arranged by size:
2144 1068 453 335 81 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 660 662 2455 660
Size: 2 1793 2293
Fragments arranged by size:
2293 1793 2
Bpu10I CC'TnA_GC
Cuts at: 688 688
Size: 4088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2635 2933 1264
Size: 879 492 298 2419
Fragments arranged by size:
2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3792 3792
Size: 4088
BsaAI yAC'GTr
Cuts at: 761 1441 761
Size: 680 3408
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 1038 1519 1767 2469 3965 1038
Size: 481 248 702 1496 1161
Fragments arranged by size:
1496 1161 702 481 248
BsaWI w'CCGG_w
Cuts at: 284 749 1277 1798 2476 2750 2897 3308 284
Size: 465 528 521 678 274 147 411 1064
Fragments arranged by size:
1064 678 528 521 465 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 256
Size: 4088
BseYI C'CCAG_C
Cuts at: 1513 2271 2848 3344 1513
Size: 758 577 496 2257
Fragments arranged by size:
2257 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2884 104
Size: 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 215 1102 1880 2070 2862 215
Size: 887 778 190 792 1441
Fragments arranged by size:
1441 887 792 778 190
BsmI GAATG_Cn'
Cuts at: 192 192
Size: 4088
Bsp1286I G_dGCh'C
Cuts at: 215 570 582 1102 1713 1806 1880 2070 2132
Size: 355 12 520 611 93 74 190 62
Cuts at: 2132 2862 215
Size: 730 1441
Fragments arranged by size:
1441 730 611 520 355 190 93 74
62 12
BspEI T'CCGG_A
Cuts at: 284 749 3308 284
Size: 465 2559 1064
Fragments arranged by size:
2559 1064 465
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 860 1138 1352 1654 2035 860
Size: 278 214 302 381 2913
Fragments arranged by size:
2913 381 302 278 214
BsrBI CCG'CTC
Cuts at: 50 2380 2434 3918 4083 50
Size: 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 2086 2267 2476 2086
Size: 181 209 3698
Fragments arranged by size:
3698 209 181
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1465
Size: 4088
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstEII G'GTnAC_C
Cuts at: 594 801 594
Size: 207 3881
BstXI CCAn_nnnn'nTGG
Cuts at: 489 489
Size: 4088
BstYI r'GATC_y
Cuts at: 1 746 1113 1580 1603 1938 2184 3196 3282
Size: 745 367 467 23 335 246 1012 86
Cuts at: 3282 3294 3640 1
Size: 12 346 449
Fragments arranged by size:
1012 745 467 449 367 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 602 107
Size: 495 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 520 2038 2219 2382 520
Size: 1518 181 163 2226
Fragments arranged by size:
2226 1518 181 163
BtsI GCAGTG_nn'
Cuts at: 343 1331 343
Size: 988 3100
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 405 479 1673 1847 2238 2265 3610 405
Size: 74 1194 174 391 27 1345 883
Fragments arranged by size:
1345 1194 883 391 174 74 27
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 355 629 913 967 2111 2321 355
Size: 274 284 54 1144 210 2122
Fragments arranged by size:
2122 1144 284 274 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 2606 2752 3898 2296
Size: 310 146 1146 2486
Fragments arranged by size:
2486 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 3092 3360 1913
Size: 432 747 268 2641
Fragments arranged by size:
2641 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1227 1227
Size: 4088
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1005 2107 1005
Size: 1102 2986
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 932 1041 1522 1770 2792 3771 932
Size: 109 481 248 1022 979 1249
Fragments arranged by size:
1249 1022 979 481 248 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 282 314 653 685 856 888 1930 1960 1962
Size: 32 339 32 171 32 1042 30 2
Cuts at: 1962 1992 282
Size: 30 2378
Fragments arranged by size:
2378 1042 339 171 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 875 1127 875
Size: 252 3836
HindIII A'AGCT_T
Cuts at: 781 781
Size: 4088
KasI G'GCGC_C
Cuts at: 1037 1518 1766 1037
Size: 481 248 3359
Fragments arranged by size:
3359 481 248
KpnI G_GTAC'C
Cuts at: 528 1108 528
Size: 580 3508
MfeI C'AATT_G
Cuts at: 419 1050 419
Size: 631 3457
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2759 2943 2759
Size: 184 3904
MscI TGG'CCA
Cuts at: 407 1849 407
Size: 1442 2646
MslI CAynn'nnrTG
Cuts at: 487 718 2077 2204 487
Size: 231 1359 127 2371
Fragments arranged by size:
2371 1359 231 127
MspA1I CmG'CkG
Cuts at: 1199 1513 1873 2886 3131 1199
Size: 314 360 1013 245 2156
Fragments arranged by size:
2156 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1038 1519 1767 1038
Size: 481 248 3359
Fragments arranged by size:
3359 481 248
NcoI C'CATG_G
Cuts at: 107 602 107
Size: 495 3593
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NspI r_CATG'y
Cuts at: 1141 1641 2548 1141
Size: 500 907 2681
Fragments arranged by size:
2681 907 500
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PmlI CAC'GTG
Cuts at: 761 761
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PshAI GACnn'nnGTC
Cuts at: 1078 1078
Size: 4088
PstI C_TGCA'G
Cuts at: 1135 1820 1135
Size: 685 3403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 1513 1873 1513
Size: 360 3728
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 873 1125 873
Size: 252 3836
SapI GCTCTTCn'nnn_
Cuts at: 355 913 2111 2321 355
Size: 558 1198 210 2122
Fragments arranged by size:
2122 1198 558 210
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 1131 1816 2809 3000 33
Size: 1098 685 993 191 1121
Fragments arranged by size:
1121 1098 993 685 191
SfoI GGC'GCC
Cuts at: 1039 1520 1768 1039
Size: 481 248 3359
Fragments arranged by size:
3359 481 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 1243 2158 2650 2912 1243
Size: 915 492 262 2419
Fragments arranged by size:
2419 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StyI C'CwwG_G
Cuts at: 107 602 1222 1576 107
Size: 495 620 354 2619
Fragments arranged by size:
2619 620 495 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 1671 1804 2263 2472 334
Size: 1337 133 459 209 1950
Fragments arranged by size:
1950 1337 459 209 133
TatI w'GTAC_w
Cuts at: 857 3603 857
Size: 2746 1342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 883 915 3605 3637 883
Size: 32 2690 32 1334
Fragments arranged by size:
2690 1334 32 32
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 732 1583 732
Size: 851 3237
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
XmnI GAAnn'nnTTC
Cuts at: 976 976
Size: 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PmlI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI